Analysis of the Authenticity of Stone Medicinal Plants by DNA Barcoding

The genus Pyrrosia belongs to the family Polypodiaceae and are medium-sized epiphytic ferns,where the dried leaves of Pyrrosia lingua,Pyrrosia sheareri,Pyrrosia lanceolata,and Pyrrosia calvata are commonly used in medicinal practice.In this study,the authenticity of the collected medicinal plant samples of Shiwei was identified with the help of DNA barcoding technology using the internal transcribed spacer 2(ITS2)as the identifying sequence.The experimental samples were analyzed using the basic local alignment search tool(BLAST)and the authenticity of the samples was further verified with the results of similarity comparison.The results proved that the sequences of the experimentally collected samples of Pyrrosia lingua,Pyrrosia sheareri,Pyrrosia lanceolata,and Pyrrosia calvata had a similarity of more than 97%when compared with the corresponding sequences that were uploaded on the Internet.

DNA Technology-assisted Signal Amplification Strategies in Electrochemiluminescence Bioanalysis

Sensitive and accurate detection of biological analytes,such as proteins,genes,small molecules,ions,cells,etc.,has been a significant project in life science.Signal amplification is one of the most effective approaches to improve the sensitivity of bioanalysis.Taking advantage of specific base pairing,programmable operation,and predictable assembly,DNA is flexible and suitable to perform the signal amplification procedure.In recent years,signal amplification strategies by means of DNA technology have been widely integrated into the construction of electrochemiluminescence(ECL)biosensors,achieving desirable analytical performance in clinical diagnosis,biomedical research,and drug development.To the best of our knowledge,these DNA signal amplification technologies mainly include classical polymerase chain reaction,and various amplification approaches conducted under mild conditions,such as rolling circle amplification(RCA)or hyperbranched RCA,cleaving enzyme-assisted amplification,DNAzyme-involved amplification,toehold-mediated DNA strand displacement amplification without enzyme participation,and so on.This review overviews the recent advancements of DNA signal amplification strategies for bioanalysis in the ECL realm,sketching the creative trajectory from strategies design to ultrasensitive ECL platform construction and resulting applications.

RAPID DETECTION OF ONCOGENE AMPLIFICATION IN PARAFFIN SECTIONS OF BREAST CARCINOMAS USING QUANTITATIVE DIFFERENTIAL PCR AND FLUORESCENT DNA TECHNOLOGY

Gene amplification is a common mechanism of oncogene activation and contributes to tumor progression. Analysis of such genetic alterations are relevant to the understanding of tumor genetics and could provide prognostic information for the individual patient. Standard analytical approaches using Southern blot and slot blot require a large amount of good

Application of Molecular Biology Technology in Identification of Modern Mongolian Medicine

The application of molecular biology technology in the identification and quality control of Mongolian medicine is increasing gradually,and it provides a new method for identifying fake and inferior products and confused products of Mongolian medicine.In this paper,the application and prospect of molecular biology technology(such as DNA barcoding and PCR molecular identification technique)in the identification of crude Mongolian medicine were reviewed.

Application of DNA Molecular Identification Method to Distinguish Ejiao and its Adulterants

Objective:To identify Ejiao and its adulterants at the DNA level by using DNA molecula marker.Ejiao(Asini Corii Colla)is a commonly used medicinal material.However,its adulteration is a serious concern.Due to the morphological characteristics of Asini Corii Colla and its adulterants,traditional identification techniques often complex and professional,which is not conducive to the circulation management and safety of the medicinal materials.To improve the distinction between Asini Corii Colla and its adulterants accurately,this study identified and its adulterant samples based on the CytB sequence.Sequence characteristics,Basic Local Alignment Search Tool(BLAST)application,genetic distance,construction of phylogenetic tree showed the CytB sequence to accurately identify Asini Corii Colla from its adulterants.Furthermore,in this study,we designed a specific primer,based on the CytB sequence,and established a PCR detection system for rapid,sensitive,and specific identification of Asini Corii Colla.Compared to DNA barcoding technology,this method has shorter detection time,stronger specificity,and higher sensitivity,which lays the foundation for the rapid identification of Asini Corii Colla.

Different in-vitro and Clinical Studies to Evaluate the Improvement of the Eyes Cream Containing the Natural Algal Complex

A natural algal complex is prepared from the brown alga Undaria pinnatifida rich in fucoidan and the red calcareous algal Corallina officinalis. The effect of the algal complex was demonstrated by transcriptomic analysis on normal human fibroblasts through the DNA chip technology from AFFYMETRIX, combined with the following in vitro Elisa test and clinical studies. Clinical studies have been performed with a basic cream containing complex versus placebo on 2 groups of 30 Caucasian women for a period of 28 days. In the present study, the natural algal complex works on the crow’s feet, eye bags, and dark cycle through multiple ways of action, including enhancing natural immune responses, regulating the inflammatory and immunity process, and promoting the extracellular matrix synthesis. As the natural algal complex has excellent improvement on the eye circumference, we have applied it to mageline firming anti-wrinkle eye cream for further research.

Gut microbiome in allogeneic hematopoietic stem cell transplantation and specific changes associated with acute graft vs host disease

Allogeneic hematopoietic stem cell transplantation(aHSCT)is a standard validated therapy for patients suffering from malignant and nonmalignant hematological diseases.However,aHSCT procedures are limited by potentially life-threatening complications,and one of the most serious complications is acute graft-versus-host disease(GVHD).During the last decades,DNA sequencing technologies were used to investigate relationship between composition or function of the gut microbiome and disease states.Even if it remains unclear whether these microbiome alterations are causative or secondary to the presence of the disease,they may be useful for diagnosis,prevention and therapy in aHSCT recipients.Here,we summarized the most recent findings of the association between human gut microbiome changes and acute GVHD in patients receiving aHSCT.

Corrigendum to Nanopore-based Fourth- generation DNA Sequencing Technology＇ [GPB 144 （2015）- GPB 13]1 （4-16）]

The authors regret that there was an incorrect citation in the Table 1 of the article ＂Nanopore-based Fourth-generation DNA Sequencing Technology＂, which was published in the journal Genomics, Proteomics ＆ Bioinformatics, Issue 1, 2015. In the table, the image in the 4th row originally appeared in the article ＂Nanopore-based sequence-specific detection of duplex DNA for geno- mic profiling＂ published by Nano Letters in 2010. Therefore, Ref. [8], which was associated with the image in the current Table l, should be replaced by the reference： Singer A, Wanunu M, Morrison W, Kuhn H, Frank-Kamenetskii M, Meller A. Nanopore- based sequence specific detection of duplex DNA for genomic profiling. Nano Lett 2010; 10：738-42.＂ The copy right permission of this article has been obtained. The authors would like to apologize for any inconvenience caused.

Corrigendum to ‘Nanopore-based Fourth-generation DNA Sequencing Technology' [GPB 144(2015)–GPB 13/1(4–16)]

The authors regret that there were typos in the online version of Figure 4 published in Issue 1,2015.In the figure legend boxes of panels F and G,‘‘Ni’’should be corrected to‘‘N’’for the labeling of the green curves.The figure in the printed version has been corrected.The correct Figure 4 is shown below.The authors would like to apologize for any inconvenience caused.

Expression of TIMP-3 Gene by Construction of a Eukaryotic Cell Expression Vector and Its Role in Reduction of Metastasis in a Human Breast Cancer Cell Line

The present study is aimed at studying the gene for TIMP-3,a mammalian tissue inhibitor,by constructing a recombinant eukaryotic cell vector for gene therapy in human breast cancer.We obtained the TIMP-3 gene from the human placent by RT-PCR.TIMP-3 gene was subcloned into pcDNA3.1 vetor from pMD18T vector by means of gene cloning to construct pcDNA3.1 recombinant vector.Human breast cancer cell line MDA-MB-453 was transfected with pcDNA3.1-TIMP3 recombinant vector using lipofectamine reagent.Then the expression of TIMP-3 and the effect on the metastasis of MDA-MB-453 were examined.The correct construction of pcDNA-TIMP3 was identified by means of restriction enzyme analysis,PCR amplication and nucleotide sequencing.Western blotting showed that the transfected cells were able to express TIMP-3, indicating that our construction of the pcDNA-TIMP3 eukaryotic expression vector was constructed successfully.Our experiments further indicated that the potential of metastasis was significantly reduced for the transfected cell line MDA-MB-453.Cellular & Molecular Immunology.2004;1(4):308-310.