乙型肝炎病毒(HBV)感染与肝细胞癌(HCC)发生关系密切,但其确切机理尚不清楚,近年来 HBV 致癌研究重点在有关 X 基因及其编码的 X 蛋白(HBVX)和 P 基因编码 DNA 聚合酶(DNA Polymerase,DNAP)上,并发现 X 基因和 P 基因的翻译产物是HBV ...乙型肝炎病毒(HBV)感染与肝细胞癌(HCC)发生关系密切,但其确切机理尚不清楚,近年来 HBV 致癌研究重点在有关 X 基因及其编码的 X 蛋白(HBVX)和 P 基因编码 DNA 聚合酶(DNA Polymerase,DNAP)上,并发现 X 基因和 P 基因的翻译产物是HBV 致癌过程中的重要因素。因此本文应用HBVX 和 DNAP 多克隆抗体对60例 HCC,展开更多
An increasing number of monopartite begomoviruses are being identified that a satellite molecule (DNAβ) is required to induce typical symptoms in host plants. DNAβ encodes a single gene (termed βC1) encoded in the ...An increasing number of monopartite begomoviruses are being identified that a satellite molecule (DNAβ) is required to induce typical symptoms in host plants. DNAβ encodes a single gene (termed βC1) encoded in the complementary-sense. We have produced transgenic Nicotiana benthamiana and N. tabacum plants expressing theβC1 gene of a DNAβ associated with Tomato yellow leaf curl China virus (TYLCCNV), under the control of the Cauliflower mosaic virus 35S promoter. Transgenic plants expressing βC1 showed severe developmental abnormalities in both species. Microscopic analysis of sections of both transgenic and non-transgenic N. tabacum leaves showed abnormal outgrowths of transgenic N. tabacum to be due to disorganized cell division (hyperplasia) of spongy and palisade parenchyma. Immuno-gold labeling of sections with a polyclonal antibody against the βC1 protein showed that the βC1 protein accumulated in the nuclei of cells. The possible biological function of the βC1 protein was discussed.展开更多
A circular DNA molecule, designated as DNAp, was identified in tobacco plants infected with Tobacco leaf curl virus (TLCV) isolates Y5 and Y8 by PCR using primers based on the conserved region of the two reported DNAp...A circular DNA molecule, designated as DNAp, was identified in tobacco plants infected with Tobacco leaf curl virus (TLCV) isolates Y5 and Y8 by PCR using primers based on the conserved region of the two reported DNAp sequences of whitefly-transmitted geminiviruses (WTGs). The complete nucleotide sequences of DNAβ of Y5 and Y8 (TLCV DNAβ) were determined. Y5 DNAβ comprises 1333 nucleotides encoding 8 predicted ORFs with 4 ORFs in virion-sense DNA and 4 ORFs in complementary-sense DNA; Y8 DNAP consists of 1338 nucleotides encoding 7 predicted ORFs with 4 ORFs in virion-sense DNA and 3 ORFs in complementary-sense DNA. TLCV DNAβhas little sequence homology to DNA-A of TLCV, except that it shares conserved TAATATTAC loop sequence with TLCV DNA-A. Sequence comparison showed that Y5 DNAβ shared 85% sequence homology with Y8 DNAβ, and both Y5 DNAβ and Y8 DNAβ had relatively low sequence identity (51%-65%) with the reported DNAβ molecules associated with Agera-tum yellow vein virus and Cotton leaf展开更多
THE duck hepatitis B virus as well as the human hepatitis B virus (HBV) belong to the hepadnaviruses. The viral DNA acts as a template for the synthesis, by DNA polymerase (DNAp). This enzyme is a DNA-dependent DNAp. ...THE duck hepatitis B virus as well as the human hepatitis B virus (HBV) belong to the hepadnaviruses. The viral DNA acts as a template for the synthesis, by DNA polymerase (DNAp). This enzyme is a DNA-dependent DNAp. It is a target enzyme of anti-hepatitis drugs too. Based on Shui Qin (SQ) anti-hepatitis B virus we used DHBV DNAp as a model in展开更多
文摘乙型肝炎病毒(HBV)感染与肝细胞癌(HCC)发生关系密切,但其确切机理尚不清楚,近年来 HBV 致癌研究重点在有关 X 基因及其编码的 X 蛋白(HBVX)和 P 基因编码 DNA 聚合酶(DNA Polymerase,DNAP)上,并发现 X 基因和 P 基因的翻译产物是HBV 致癌过程中的重要因素。因此本文应用HBVX 和 DNAP 多克隆抗体对60例 HCC,
文摘An increasing number of monopartite begomoviruses are being identified that a satellite molecule (DNAβ) is required to induce typical symptoms in host plants. DNAβ encodes a single gene (termed βC1) encoded in the complementary-sense. We have produced transgenic Nicotiana benthamiana and N. tabacum plants expressing theβC1 gene of a DNAβ associated with Tomato yellow leaf curl China virus (TYLCCNV), under the control of the Cauliflower mosaic virus 35S promoter. Transgenic plants expressing βC1 showed severe developmental abnormalities in both species. Microscopic analysis of sections of both transgenic and non-transgenic N. tabacum leaves showed abnormal outgrowths of transgenic N. tabacum to be due to disorganized cell division (hyperplasia) of spongy and palisade parenchyma. Immuno-gold labeling of sections with a polyclonal antibody against the βC1 protein showed that the βC1 protein accumulated in the nuclei of cells. The possible biological function of the βC1 protein was discussed.
基金This work was partially supported by the National Outstanding Youth Foundation (Grant No.30125032) the Teaching and Research Award Program for Outstanding Young Teachers in Higher Education Institutions of MOE also by the National Natural Science F
文摘A circular DNA molecule, designated as DNAp, was identified in tobacco plants infected with Tobacco leaf curl virus (TLCV) isolates Y5 and Y8 by PCR using primers based on the conserved region of the two reported DNAp sequences of whitefly-transmitted geminiviruses (WTGs). The complete nucleotide sequences of DNAβ of Y5 and Y8 (TLCV DNAβ) were determined. Y5 DNAβ comprises 1333 nucleotides encoding 8 predicted ORFs with 4 ORFs in virion-sense DNA and 4 ORFs in complementary-sense DNA; Y8 DNAP consists of 1338 nucleotides encoding 7 predicted ORFs with 4 ORFs in virion-sense DNA and 3 ORFs in complementary-sense DNA. TLCV DNAβhas little sequence homology to DNA-A of TLCV, except that it shares conserved TAATATTAC loop sequence with TLCV DNA-A. Sequence comparison showed that Y5 DNAβ shared 85% sequence homology with Y8 DNAβ, and both Y5 DNAβ and Y8 DNAβ had relatively low sequence identity (51%-65%) with the reported DNAβ molecules associated with Agera-tum yellow vein virus and Cotton leaf
文摘THE duck hepatitis B virus as well as the human hepatitis B virus (HBV) belong to the hepadnaviruses. The viral DNA acts as a template for the synthesis, by DNA polymerase (DNAp). This enzyme is a DNA-dependent DNAp. It is a target enzyme of anti-hepatitis drugs too. Based on Shui Qin (SQ) anti-hepatitis B virus we used DHBV DNAp as a model in
