Bacterial adherence to the acquired dental pellicle, important in dental caries (caries), is mediated by receptor-adhesins such as salivary agglutinin binding to Streptococcus mutans antigen I/II (I/II). Ten selected ...Bacterial adherence to the acquired dental pellicle, important in dental caries (caries), is mediated by receptor-adhesins such as salivary agglutinin binding to Streptococcus mutans antigen I/II (I/II). Ten selected I/II epitopes were chosen to determine their reactivity to human salivary IgA. Previous studies suggested that a specific HLA biomarker group (HLA-DRB1*04) may have differential influence of immune responses to I/II. However, it was not known whether secretory IgA (SIgA) responses to the selected epitopes from HLA-DRB1*04 positive subjects were different compared to controls, or across other caries-related factors such as total IgA (TIgA). Thirty-two total subjects were matched according to HLA type, gender, ethnicity and age. HLA genotyping, oral bacterial, immunoglobulin and antibody analyses were performed. A large observed difference emerged with regard to the natural immune reservoir of TIgA in HLA-DRB1*04 positive subjects, specifically, a 27.6% reduction compared to controls. In contrast to all other epitopes studied, HLA-DRB1*04 positive subjects also exhibited reduced reactivity to I/II epitope 834-853. HLA-DRB1*04 positive subjects exhibited lower specific SIgA activity/TIgA to 834-853 and also a lower specific reactivity to 834-853/whole cell S. mutans UA159. Furthermore, HLA-DRB1*04 positive subjects exhibited lower responses to I/II in its entirety. The large observed difference in TIgA and the 834-853 reactivity pattern across multiple measures suggest potentially important connections pertaining to the link between HLA-DRB1*04 and caries.展开更多
目的从分子遗传学角度探讨乙型肝炎病毒（hepatitis B virus，HBv）感染结局与GLA—DRBl＊04等位基因的相关性。方法采用聚合酶链反应一序列特异性引物（polymerase chain reaction-sequence specific primers，PCR-SSP）技术检测HLA-D...目的从分子遗传学角度探讨乙型肝炎病毒（hepatitis B virus，HBv）感染结局与GLA—DRBl＊04等位基因的相关性。方法采用聚合酶链反应一序列特异性引物（polymerase chain reaction-sequence specific primers，PCR-SSP）技术检测HLA-DRBl＊04等位基因，并比较106例无症状HBV携带者（HBV携带组），93例慢性乙型肝炎患者、77例乙肝肝硬化者、102例HBV感染后自然恢复者（对照组）HIA-DRBl＊04等位基因频率及HBV不同复制状态下HLA-DRBl＊04等位基因频率。结果HBV携带组、慢性乙肝组、乙肝肝硬化组HLA-DRBl＊04等位基因频率高于对照组（分别为25．94％、26．34％、27．92％和14．22％，P〈0．01）；HLA—DRBl＊0401基因频率高于对照组（分别为20．91％、24．49％、22．09％和8．62％，P均〈0．05）；HLA-DRBl＊0405基因频率较对照组低（3．64％、2．04％、3．49％和15．52％，P〈0．01、0．01、0．05）。各病例组之间HLA—DRBl＊04等位基因频率差异无统计学意义（P〉0．05）。HBV不同复制状态HLA-DRBl＊04等位基因频率差异无统计学意义（P〉O．05）。结论HLA-DRBl＊04是决定HBV感染结局的因素之一，但不影响HBV在体内复制。展开更多
目的多种抗癫痫药物(AEDs)所致皮肤不良反应(cutaneous adverse drug reactions,cADRs)发现与HLA等位基因密切相关。但研究主要集中在对传统AEDs的研究上,而对新型AEDs,特别是对于左乙拉西坦(Levetiracetam,LEV)所致cADRs与HLA等位基因...目的多种抗癫痫药物(AEDs)所致皮肤不良反应(cutaneous adverse drug reactions,cADRs)发现与HLA等位基因密切相关。但研究主要集中在对传统AEDs的研究上,而对新型AEDs,特别是对于左乙拉西坦(Levetiracetam,LEV)所致cADRs与HLA等位基因相关性的研究较少。因此,研究探索LEV所致cADRs的临床特点,以及其与HLA等位基因之间可能的相关性。方法纳入2011年9月-2014年12月期间于四川大学华西医院门诊及住院部连续登记的LEV所致cADRs患者共9例,详细收集患者的流行病学信息及临床资料。同时,按1∶4的比例收集服用LEV未出现过敏反应的患者组成对照组。每例受试者均进行HLA-A,-B和-DRB1位点的高分辨率分析,并对每种基因在病例组和对照组中的携带率进行比较。结果 9例服用LEV出现cADRs的患者组成病例组,36例耐受LEV的患者组成对照组。全部LEV所致的cADRs均为轻度皮疹,且出现在用药的30 d内。从使用LEV到出现皮疹的平均天数为(15.67±5.41)d(范围:6~27 d)。病例组中,有2例患者(2/9,22.2%)携带HLA-DRB1*04:05等位基因,对照组中没有患者携带该基因,HLADRB1*04:05等位基因的携带率在病例组和对照组的差异有统计学意义[P=0.036,OR=13.875,95%CI(1.273,151.230)]。结论虽然LEV是较为安全、cADRs发生频率较低的AEDs,但使用LEV治疗癫痫时仍应密切监测cADRs的发生,特别是在起始用药的4周内。研究提示HLA-DRB1*04:05等位基因可能是LEV所致cADRs的危险因素之一。由于研究样本量较小,仍需大样本研究进一步证实。展开更多
文摘Bacterial adherence to the acquired dental pellicle, important in dental caries (caries), is mediated by receptor-adhesins such as salivary agglutinin binding to Streptococcus mutans antigen I/II (I/II). Ten selected I/II epitopes were chosen to determine their reactivity to human salivary IgA. Previous studies suggested that a specific HLA biomarker group (HLA-DRB1*04) may have differential influence of immune responses to I/II. However, it was not known whether secretory IgA (SIgA) responses to the selected epitopes from HLA-DRB1*04 positive subjects were different compared to controls, or across other caries-related factors such as total IgA (TIgA). Thirty-two total subjects were matched according to HLA type, gender, ethnicity and age. HLA genotyping, oral bacterial, immunoglobulin and antibody analyses were performed. A large observed difference emerged with regard to the natural immune reservoir of TIgA in HLA-DRB1*04 positive subjects, specifically, a 27.6% reduction compared to controls. In contrast to all other epitopes studied, HLA-DRB1*04 positive subjects also exhibited reduced reactivity to I/II epitope 834-853. HLA-DRB1*04 positive subjects exhibited lower specific SIgA activity/TIgA to 834-853 and also a lower specific reactivity to 834-853/whole cell S. mutans UA159. Furthermore, HLA-DRB1*04 positive subjects exhibited lower responses to I/II in its entirety. The large observed difference in TIgA and the 834-853 reactivity pattern across multiple measures suggest potentially important connections pertaining to the link between HLA-DRB1*04 and caries.
文摘目的从分子遗传学角度探讨乙型肝炎病毒（hepatitis B virus，HBv）感染结局与GLA—DRBl＊04等位基因的相关性。方法采用聚合酶链反应一序列特异性引物（polymerase chain reaction-sequence specific primers，PCR-SSP）技术检测HLA-DRBl＊04等位基因，并比较106例无症状HBV携带者（HBV携带组），93例慢性乙型肝炎患者、77例乙肝肝硬化者、102例HBV感染后自然恢复者（对照组）HIA-DRBl＊04等位基因频率及HBV不同复制状态下HLA-DRBl＊04等位基因频率。结果HBV携带组、慢性乙肝组、乙肝肝硬化组HLA-DRBl＊04等位基因频率高于对照组（分别为25．94％、26．34％、27．92％和14．22％，P〈0．01）；HLA—DRBl＊0401基因频率高于对照组（分别为20．91％、24．49％、22．09％和8．62％，P均〈0．05）；HLA-DRBl＊0405基因频率较对照组低（3．64％、2．04％、3．49％和15．52％，P〈0．01、0．01、0．05）。各病例组之间HLA—DRBl＊04等位基因频率差异无统计学意义（P〉0．05）。HBV不同复制状态HLA-DRBl＊04等位基因频率差异无统计学意义（P〉O．05）。结论HLA-DRBl＊04是决定HBV感染结局的因素之一，但不影响HBV在体内复制。
