Background: Gamma-glutamyltransferase is recognised as a biomarker to assess the harms associated with alcohol misuse. The objective ways to measure GGT in areas lacking central lab facilities are desirable. This stud...Background: Gamma-glutamyltransferase is recognised as a biomarker to assess the harms associated with alcohol misuse. The objective ways to measure GGT in areas lacking central lab facilities are desirable. This study aims to measure GGT from dried serum spots and its storage from dried serum spots. Method: The study was approved by the institutional ethical committee. One hundred and eighty (180) patients were included in the study. Their blood samples were collected. The serum samples were spotted onto filter paper (Whatman 903) dried and stored at 4°C. The GGT levels were measured on the day of collection and at various time periods to assess the effect of storage. All the analysis was performed on SPSS version 21. Result: The GGT levels measured from fresh serum GGT levels mean (SD) 286.5 (539.4) correlated well with their respective dried serum levels 287.18 (538.2) (P = 0.80). The mean recovery of GGT from dried serum was observed to be 103.3%. A sub-sample (n = 12) was stored at 4°C. The dried serum spots were found to be stable at the end of four weeks using repeated measure analysis of variance (ANOVA) (P = 0.39). Conclusion: This method has the potential to be used for epidemiological or field based studies to assess harms associated with alcohol use.展开更多
Inorganic nitrogen(N)loss through sediment N mineralization is important for eutrophication surrounding riparian zone.Sediment physicochemical properties have been changed at water-level elevation in riparian zone of ...Inorganic nitrogen(N)loss through sediment N mineralization is important for eutrophication surrounding riparian zone.Sediment physicochemical properties have been changed at water-level elevation in riparian zone of the Three Gorges Reservoir(TGR)due to differences in hydrological stress and human activity intensity.However,spatial distribution and driving factor of net N mineralization rate(Nmin)and its temperature sensitivity(Q10)based on the changes in sediment physicochemical properties are still unclear at waterlevel elevation in the riparian zone.A total of 132 sediment samples in the riparian zone were collected including 11 transections and 12 water-level elevations on basin scale of the TGR during drying period,to conduct a 28-day incubation at 15℃,22℃,29℃and 36℃.Nmin,total N(TN)and substrate quality(SQ)increased with water-level elevation,while Q10 showed an opposite trend(P<0.001).Results of the structural equation model showed that water-level elevation had direct positive effects on TN and SQ(P<0.01).In addition,TN was the major factor that had a direct positive effect on Nmin,and SQ was the crucial factor that had a direct negative effect on Q10(P<0.001).In conclusion,increases in TN and SQ were major driving factors of Nmin and its Q10 at water-level elevation,respectively,in riparian zone of the TGR during drying period.展开更多
文摘Background: Gamma-glutamyltransferase is recognised as a biomarker to assess the harms associated with alcohol misuse. The objective ways to measure GGT in areas lacking central lab facilities are desirable. This study aims to measure GGT from dried serum spots and its storage from dried serum spots. Method: The study was approved by the institutional ethical committee. One hundred and eighty (180) patients were included in the study. Their blood samples were collected. The serum samples were spotted onto filter paper (Whatman 903) dried and stored at 4°C. The GGT levels were measured on the day of collection and at various time periods to assess the effect of storage. All the analysis was performed on SPSS version 21. Result: The GGT levels measured from fresh serum GGT levels mean (SD) 286.5 (539.4) correlated well with their respective dried serum levels 287.18 (538.2) (P = 0.80). The mean recovery of GGT from dried serum was observed to be 103.3%. A sub-sample (n = 12) was stored at 4°C. The dried serum spots were found to be stable at the end of four weeks using repeated measure analysis of variance (ANOVA) (P = 0.39). Conclusion: This method has the potential to be used for epidemiological or field based studies to assess harms associated with alcohol use.
基金supported by the Program of Chongqing Science and Technology Commission(cstc2020jcyj-msxmX0095)the Science and Technology Research Program of Chongqing Municipal Education Commission(KJZDK202001203,KJZD-K202003501)the Innovative Research Group of Universities in Chongqing(CXQT P19037).
文摘Inorganic nitrogen(N)loss through sediment N mineralization is important for eutrophication surrounding riparian zone.Sediment physicochemical properties have been changed at water-level elevation in riparian zone of the Three Gorges Reservoir(TGR)due to differences in hydrological stress and human activity intensity.However,spatial distribution and driving factor of net N mineralization rate(Nmin)and its temperature sensitivity(Q10)based on the changes in sediment physicochemical properties are still unclear at waterlevel elevation in the riparian zone.A total of 132 sediment samples in the riparian zone were collected including 11 transections and 12 water-level elevations on basin scale of the TGR during drying period,to conduct a 28-day incubation at 15℃,22℃,29℃and 36℃.Nmin,total N(TN)and substrate quality(SQ)increased with water-level elevation,while Q10 showed an opposite trend(P<0.001).Results of the structural equation model showed that water-level elevation had direct positive effects on TN and SQ(P<0.01).In addition,TN was the major factor that had a direct positive effect on Nmin,and SQ was the crucial factor that had a direct negative effect on Q10(P<0.001).In conclusion,increases in TN and SQ were major driving factors of Nmin and its Q10 at water-level elevation,respectively,in riparian zone of the TGR during drying period.
