Soybean is a photoperiod-sensitive short-day crop whose reproductive period and yield are markedly affected by day-length changes.Seed weight is one of the key traits determining the soybean yield;how-ever,the promine...Soybean is a photoperiod-sensitive short-day crop whose reproductive period and yield are markedly affected by day-length changes.Seed weight is one of the key traits determining the soybean yield;how-ever,the prominent genes that control the final seed weight of soybean and the mechanisms underlying the photoperiod's effect on this trait remain poorly understood.In this study,we identify SwW19 as a major locus controlling soybean seed weight by QTL mapping and determine Dt1,an orthologous gene of Arabidopsis TFL1 that is known to govern the soybean growth habit,as the causal gene of the SW19 locus.We showed that Dt1 is highly expressed in developing seeds and regulates photoperiod-dependent seed weight in soybean.Further analyses revealed that the Dt1 protein physically interacts with the sucrose transporter GmSWEET10a to negatively regulate the import of sucrose from seed coat to the embryo,thus modulating seed weight under long days.However,Dt1 does not function in seed development under short days due to its very low expression.Importantly,we discovered a novel natural allelic variant of Dt1(H4 haplotype)that decouples its pleiotropic effects on seed size and growth habit;i.e.,this variant remains functional in seed development but fails to regulate the stem growth habit of soybean.Collectively,our findings provide new insights into how soybean seed development responds to photoperiod at different latitudes,offering an ideal genetic component for improving soybean's yield by manipulating its seed weightandgrowth habit.展开更多
Flowering time and stem growth habit determine inflorescence architecture in soybean, which in turn influences seed yield. Dt1, a homolog of Arabidopsis TERMINAL FLOWER 1(TFL1), is a major controller of stem growth ha...Flowering time and stem growth habit determine inflorescence architecture in soybean, which in turn influences seed yield. Dt1, a homolog of Arabidopsis TERMINAL FLOWER 1(TFL1), is a major controller of stem growth habit, but its underlying molecular mechanisms remain unclear.Here, we demonstrate that Dt1 affects node number and plant height, as well as flowering time,in soybean under long-day conditions. The b ZIP transcription factor FDc1 physically interacts with Dt1, and the FDc1-Dt1 complex directly represses the expression of APETALA1(AP1). We propose that FT5 a inhibits Dt1 activity via a competitive interaction with FDc1 and directly upregulates AP1. Moreover, AP1 represses Dt1 expression by directly binding to the Dt1 promoter, suggesting that AP1 and Dt1 form a suppressive regulatory feedback loop to determine the fate of the shoot apical meristem. These findings provide novel insights into the roles of Dt1 and FT5 a in controlling the stem growth habit and flowering time in soybean, which determine the adaptability and grain yield of this important crop.展开更多
A new method for preparing effective inhibition film on copper has been developed. Phenylthiourea (PT) was first absorbed to copper surface to form a monolayer. 1-Dodecanethiol (DT) was then assembled on the surface...A new method for preparing effective inhibition film on copper has been developed. Phenylthiourea (PT) was first absorbed to copper surface to form a monolayer. 1-Dodecanethiol (DT) was then assembled on the surface for modification. Finally, AC voltage was loaded on copper covered the mixed film to improve it further. After these processes, an effective inhibition film was gained because of its high charge transfer resistance and low corrosion current density shown in electrochemical impedance spectra and polarization. The inhibition efficiency was more than 97%.展开更多
An L（d0,d2,...,dt）-labeling of a graph G is a function f from its vertex set V（G） to the set {0,1,..., k} for some positive integer k such that If（x） - f（y）l ≥di, if the distance between vertices x and y in G...An L（d0,d2,...,dt）-labeling of a graph G is a function f from its vertex set V（G） to the set {0,1,..., k} for some positive integer k such that If（x） - f（y）l ≥di, if the distance between vertices x and y in G is equal to i for i = 1,2,...,t. The L（d1,d2,...,dt）-number λ（G;d1,d2,... ,dt） of G is the smallest integer number k such that G has an L（d1,d2,...,dr）- labeling with max{f （x）｜x ∈ V（G）} = k. In this paper, we obtain the exact values for λ（Cn; 2, 2, 1） and λ（Cn; 3, 2, 1）, and present lower and upper bounds for λ（Cn; 2,..., 2, 1,..., 1）展开更多
基金supported by the National Natural Science Foundation of China(grant no.32090064 to F.K.)the National Key R&D Program of China(2021YFF1001203 to X.H.)+3 种基金the“Strategic Priority Research Program"of the Chinese Academy of Sciences(grant no.XDA24010105 to X.H.)the National Natural Science Foundation of China(grant nos.32230078 and 31871643 to X.H.)the Major Program of Guangdong Basic and Applied Research(grant no.2019B030302006 to F.K.)the Guangzhou Municipal Science and Technology Project(grant no.202201010641toY.H.).
文摘Soybean is a photoperiod-sensitive short-day crop whose reproductive period and yield are markedly affected by day-length changes.Seed weight is one of the key traits determining the soybean yield;how-ever,the prominent genes that control the final seed weight of soybean and the mechanisms underlying the photoperiod's effect on this trait remain poorly understood.In this study,we identify SwW19 as a major locus controlling soybean seed weight by QTL mapping and determine Dt1,an orthologous gene of Arabidopsis TFL1 that is known to govern the soybean growth habit,as the causal gene of the SW19 locus.We showed that Dt1 is highly expressed in developing seeds and regulates photoperiod-dependent seed weight in soybean.Further analyses revealed that the Dt1 protein physically interacts with the sucrose transporter GmSWEET10a to negatively regulate the import of sucrose from seed coat to the embryo,thus modulating seed weight under long days.However,Dt1 does not function in seed development under short days due to its very low expression.Importantly,we discovered a novel natural allelic variant of Dt1(H4 haplotype)that decouples its pleiotropic effects on seed size and growth habit;i.e.,this variant remains functional in seed development but fails to regulate the stem growth habit of soybean.Collectively,our findings provide new insights into how soybean seed development responds to photoperiod at different latitudes,offering an ideal genetic component for improving soybean's yield by manipulating its seed weightandgrowth habit.
基金funded by the Major Program of Guangdong Basic and Applied Research(2019B030302006)supported by the National Natural Science Foundation of China(31930083,31901567,31901499,31801384)the China Postdoctoral Science Foundation(2019M662843,2019M652839,2019M662842)。
文摘Flowering time and stem growth habit determine inflorescence architecture in soybean, which in turn influences seed yield. Dt1, a homolog of Arabidopsis TERMINAL FLOWER 1(TFL1), is a major controller of stem growth habit, but its underlying molecular mechanisms remain unclear.Here, we demonstrate that Dt1 affects node number and plant height, as well as flowering time,in soybean under long-day conditions. The b ZIP transcription factor FDc1 physically interacts with Dt1, and the FDc1-Dt1 complex directly represses the expression of APETALA1(AP1). We propose that FT5 a inhibits Dt1 activity via a competitive interaction with FDc1 and directly upregulates AP1. Moreover, AP1 represses Dt1 expression by directly binding to the Dt1 promoter, suggesting that AP1 and Dt1 form a suppressive regulatory feedback loop to determine the fate of the shoot apical meristem. These findings provide novel insights into the roles of Dt1 and FT5 a in controlling the stem growth habit and flowering time in soybean, which determine the adaptability and grain yield of this important crop.
基金Subsidized with the Special Funds for the Major State Basic Research Projects G19990650 and the Chinese National Science Fund (No. 20173033).
文摘A new method for preparing effective inhibition film on copper has been developed. Phenylthiourea (PT) was first absorbed to copper surface to form a monolayer. 1-Dodecanethiol (DT) was then assembled on the surface for modification. Finally, AC voltage was loaded on copper covered the mixed film to improve it further. After these processes, an effective inhibition film was gained because of its high charge transfer resistance and low corrosion current density shown in electrochemical impedance spectra and polarization. The inhibition efficiency was more than 97%.
基金the National Natural Science Foundation of China (No.10531070)the National Basic Research Program of China 973 Program (No.2006AA11Z209)the Natural Science Foundation of Shanghai City (No.06ZR14049)
文摘An L（d0,d2,...,dt）-labeling of a graph G is a function f from its vertex set V（G） to the set {0,1,..., k} for some positive integer k such that If（x） - f（y）l ≥di, if the distance between vertices x and y in G is equal to i for i = 1,2,...,t. The L（d1,d2,...,dt）-number λ（G;d1,d2,... ,dt） of G is the smallest integer number k such that G has an L（d1,d2,...,dr）- labeling with max{f （x）｜x ∈ V（G）} = k. In this paper, we obtain the exact values for λ（Cn; 2, 2, 1） and λ（Cn; 3, 2, 1）, and present lower and upper bounds for λ（Cn; 2,..., 2, 1,..., 1）
