矮脆突变体dwf1(dwarf and fragile 1)来源于EMS诱变处理的籼型恢复系缙恢10号,主要表现为根、茎、叶、叶鞘、子粒等器官特别脆,同时植株变矮、叶片披垂。株高、穗长、结实率、节间长以及千粒重有不同程度降低,细胞壁中纤维素和木质素...矮脆突变体dwf1(dwarf and fragile 1)来源于EMS诱变处理的籼型恢复系缙恢10号,主要表现为根、茎、叶、叶鞘、子粒等器官特别脆,同时植株变矮、叶片披垂。株高、穗长、结实率、节间长以及千粒重有不同程度降低,细胞壁中纤维素和木质素含量下降、半纤维素含量增加,机械强度下降。茎秆表面锯齿状突起尖锐,薄壁细胞较野生型小、细胞大小不一致、排列紊乱,细胞形状不规则、长度稍有变短。该突变性状受一对隐性核基因控制,位于第9染色体上标记Ind6与Ind4之间,dwf1相对于野生型在LOC_Os09g25490第7外显子上有一个碱基的错义突变,导致氨基酸由半胱氨酸突变为精氨酸,该突变发生在基因的高度保守区域内。dwf1对深入研究水稻变矮变脆机制具有重要意义。展开更多
Brassinosteroids（BRs）, a class of steroidal phytohormones are essential for many biological processes in plant. However, little is known about their roles in fruit development. Tomato is a highly valuable vegetable ...Brassinosteroids（BRs）, a class of steroidal phytohormones are essential for many biological processes in plant. However, little is known about their roles in fruit development. Tomato is a highly valuable vegetable and has been adopted as the model species for studying fruit growth, development, and ripening. To understand the role of endogenous BRs in the development of tomato fruit, the expression patterns of three homologues of DWF4 gene were investigated and the transgenic tomato plants were generated in which the Gh DWF4 gene from upland cotton（Gossypium hirsutum L.） was ectopically expressed. The contents of main quality components were analyzed in fruits of transgenic tomato line and non-transgenic line（control plant, CP） when the fruit was mature. Sl CYP90B3 that possesses high homology with Gh DWF4 preferentially expressed in mature fruit. Significantly higher contents of soluble sugar, soluble proteins, and vitamin C were obtained in fruit of transgenic tomato lines compared with those in the CP. Furthermore, overexpressing Gh DWF4 promoted fruit growth and ripening. The weight per fruit was increased by about 23% in transgenic lines. In addition, overexpressing Gh DWF4 promoted the germination of transgenic tomato seeds and hypocotyl elongation of seedlings. These results indicated that overexpressing Gh DWF4 gene in tomato could increase the contents of many nutrients in fruit and accelerate fruit ripening. It is suggested that increased endogenous BRs in fruit affect the growth and development of tomato fruit and therefore improved the nutrient quality of tomato.展开更多
作为世界领先的设计和数字内部创建资源提供商欧特克近日宣布与微软公司合作，通过运用XPS（XML Paper Specification）技术，实现DWF技术与Windows Vista平台的完美结合。今后，用户只要将DWF文件以XPS格式发行，就可直接在Windows Vi...作为世界领先的设计和数字内部创建资源提供商欧特克近日宣布与微软公司合作，通过运用XPS（XML Paper Specification）技术，实现DWF技术与Windows Vista平台的完美结合。今后，用户只要将DWF文件以XPS格式发行，就可直接在Windows Vista中使用XPS阅读器，自动打开、浏览并管理DWF文件的详细数据，而无需额外下载插件或其他特定的看图软件。欧特克与微软此次的战略合作，将为从设计到执行的各个团队，提供更加方便的方式共享设计信息，实现高效率的协同作业，从而彻底地提升工作效率与生产力。展开更多
文摘矮脆突变体dwf1(dwarf and fragile 1)来源于EMS诱变处理的籼型恢复系缙恢10号,主要表现为根、茎、叶、叶鞘、子粒等器官特别脆,同时植株变矮、叶片披垂。株高、穗长、结实率、节间长以及千粒重有不同程度降低,细胞壁中纤维素和木质素含量下降、半纤维素含量增加,机械强度下降。茎秆表面锯齿状突起尖锐,薄壁细胞较野生型小、细胞大小不一致、排列紊乱,细胞形状不规则、长度稍有变短。该突变性状受一对隐性核基因控制,位于第9染色体上标记Ind6与Ind4之间,dwf1相对于野生型在LOC_Os09g25490第7外显子上有一个碱基的错义突变,导致氨基酸由半胱氨酸突变为精氨酸,该突变发生在基因的高度保守区域内。dwf1对深入研究水稻变矮变脆机制具有重要意义。
基金supported by the Natural Science Foundation of Chongqing, China (CSTC, 2011BB1007)the Genetically Modified Organisms Breeding Major Projects of China (2009ZX08009-118B)
文摘Brassinosteroids（BRs）, a class of steroidal phytohormones are essential for many biological processes in plant. However, little is known about their roles in fruit development. Tomato is a highly valuable vegetable and has been adopted as the model species for studying fruit growth, development, and ripening. To understand the role of endogenous BRs in the development of tomato fruit, the expression patterns of three homologues of DWF4 gene were investigated and the transgenic tomato plants were generated in which the Gh DWF4 gene from upland cotton（Gossypium hirsutum L.） was ectopically expressed. The contents of main quality components were analyzed in fruits of transgenic tomato line and non-transgenic line（control plant, CP） when the fruit was mature. Sl CYP90B3 that possesses high homology with Gh DWF4 preferentially expressed in mature fruit. Significantly higher contents of soluble sugar, soluble proteins, and vitamin C were obtained in fruit of transgenic tomato lines compared with those in the CP. Furthermore, overexpressing Gh DWF4 promoted fruit growth and ripening. The weight per fruit was increased by about 23% in transgenic lines. In addition, overexpressing Gh DWF4 promoted the germination of transgenic tomato seeds and hypocotyl elongation of seedlings. These results indicated that overexpressing Gh DWF4 gene in tomato could increase the contents of many nutrients in fruit and accelerate fruit ripening. It is suggested that increased endogenous BRs in fruit affect the growth and development of tomato fruit and therefore improved the nutrient quality of tomato.
文摘作为世界领先的设计和数字内部创建资源提供商欧特克近日宣布与微软公司合作，通过运用XPS（XML Paper Specification）技术，实现DWF技术与Windows Vista平台的完美结合。今后，用户只要将DWF文件以XPS格式发行，就可直接在Windows Vista中使用XPS阅读器，自动打开、浏览并管理DWF文件的详细数据，而无需额外下载插件或其他特定的看图软件。欧特克与微软此次的战略合作，将为从设计到执行的各个团队，提供更加方便的方式共享设计信息，实现高效率的协同作业，从而彻底地提升工作效率与生产力。
