Diversity in bacterial communities was investigated along a petroleum hydrocarbon content gradient(0-0.4043 g/g)in surface(5-10 cm)and subsurface(35-40 cm)petroleum-contaminated soil samples from the Dagang Oilfield,C...Diversity in bacterial communities was investigated along a petroleum hydrocarbon content gradient(0-0.4043 g/g)in surface(5-10 cm)and subsurface(35-40 cm)petroleum-contaminated soil samples from the Dagang Oilfield,China.Using 16S rRNA Illumina high-throughput sequencing technology and several statistical methods,the bacterial diversity of the soil was studied.Subsequently,the environmental parameters were measured to analyze its relationship with the community variation.Nonmetric multidimensional scaling and analysis of similarities indicated a significant difference in the structure of the bacterial community between the nonpetroleum-contaminated surface and subsurface soils,but no differences were observed in different depths of petroleum-contaminated soil.Meanwhile,many significant correlations were obtained between diversity in soil bacterial community and physicochemical properties.Total petroleum hydrocarbon,total organic carbon,and total nitrogen were the three important factors that had the greatest impacts on the bacterial community distribution in the long-term petroleum-contaminated soils.Our research has provided references for the bacterial community distribution along a petroleum gradient in both surface and subsurface petroleum-contaminated soils of oilfield areas.展开更多
Dagang Oilfield is one of the 100 extra-large enterprises in China,with production and processing of petroleum and natural gas as its prior1-ty.After 30 years of exploitation and construction,it has become a backbone ...Dagang Oilfield is one of the 100 extra-large enterprises in China,with production and processing of petroleum and natural gas as its prior1-ty.After 30 years of exploitation and construction,it has become a backbone oilfield in East China.At present,it has 63000 workers and staff,fixed as-sets of 5 billion yuan,and annual output of crude oil 4 million tons and natural gas 360 million cubic me-ters.展开更多
基金supported by the Major Research Plan of Tianjin (No.16YFXTSF00460)the National Natural Science Foundation of China (No.21878220)
文摘Diversity in bacterial communities was investigated along a petroleum hydrocarbon content gradient(0-0.4043 g/g)in surface(5-10 cm)and subsurface(35-40 cm)petroleum-contaminated soil samples from the Dagang Oilfield,China.Using 16S rRNA Illumina high-throughput sequencing technology and several statistical methods,the bacterial diversity of the soil was studied.Subsequently,the environmental parameters were measured to analyze its relationship with the community variation.Nonmetric multidimensional scaling and analysis of similarities indicated a significant difference in the structure of the bacterial community between the nonpetroleum-contaminated surface and subsurface soils,but no differences were observed in different depths of petroleum-contaminated soil.Meanwhile,many significant correlations were obtained between diversity in soil bacterial community and physicochemical properties.Total petroleum hydrocarbon,total organic carbon,and total nitrogen were the three important factors that had the greatest impacts on the bacterial community distribution in the long-term petroleum-contaminated soils.Our research has provided references for the bacterial community distribution along a petroleum gradient in both surface and subsurface petroleum-contaminated soils of oilfield areas.
文摘Dagang Oilfield is one of the 100 extra-large enterprises in China,with production and processing of petroleum and natural gas as its prior1-ty.After 30 years of exploitation and construction,it has become a backbone oilfield in East China.At present,it has 63000 workers and staff,fixed as-sets of 5 billion yuan,and annual output of crude oil 4 million tons and natural gas 360 million cubic me-ters.
