Objective:To explore the mechanism and related active components of Yishen Daluo decoction(YSDLD)in treating multiple sclerosis(MS).Methods:Targets of YSDLD were collected through the TCMSP,Chemistry,and TCMID databas...Objective:To explore the mechanism and related active components of Yishen Daluo decoction(YSDLD)in treating multiple sclerosis(MS).Methods:Targets of YSDLD were collected through the TCMSP,Chemistry,and TCMID databases.The MS targets were collected through OMIM,DrugBank,Gencards,TTD,and Pharmgkb databases.We built“componentetarget”network diagrams and proteineprotein interaction(PPI)diagrams and performed topological analysis.The targets were subjected to GO and KEGG enrichment analysis.Molecular docking verification was conducted on selected targets and molecules.Finally,in vitro experiments were con-ducted.BV2 cells were induced by lipopolysaccharide for model establishment.CCK8 experiment was conducted to explore the effect of YSDLD and RT-qPCR technology was used to explore the expression of key targets.Results:There were 184 active components in YSDLD and 898 targets of its action.There were 940 MS targets,and 215 targets were shared by YSDLD and MS.According to the“componentetarget”diagram,the top five key components included quercetin,kaempferol,beta-sitosterol,stigmasterol,and nar-ingenin.IL-6,IL-1 b,TNF-α,AKT1,and VEGFA were the important targets identified by PPI network to-pology analysis.A total of 564 functions were identified by GO enrichment analysis(P<0.01),mainly involving inflammatory response,hypoxia response,plasma membrane,neuronal cell body,protein phosphatase binding,and cytokine activity.KEGG enrichment analysis enriched 98 pathways(P<.01).YSDLD at the concentration of 20 m g/mL had no effect on BV2 cells.RT-qPCR indicated that YSDLD at the concentrations of 15 m g/mL and 20 m g/mL alleviated LPS-induced inflammatory injury and lowered the content of inflammatory factors(P<0.05).Conclusion:In this paper,the network pharmacology and in vitro experiments were used to explore the potential mechanism of YSDLD in treating MS.The research provides a good basis for the development of YSDLD and drugs for MS in future.展开更多
Daluo Mountains lie at front of the arcuate tectonic belt at the northeastern margin of the Tibetan Plateau,and are the landform boundary zone between the active Tibetan Plateau and the stable North China Craton.Study...Daluo Mountains lie at front of the arcuate tectonic belt at the northeastern margin of the Tibetan Plateau,and are the landform boundary zone between the active Tibetan Plateau and the stable North China Craton.Studying of the late Cenozoic uplift evolution of Daluo Mountains is important for understanding the expansion mechanism of the northeastern margin of the Tibetan Plateau and its influence on the western North China Craton.In this study,the late Cenozoic uplift of Daluo Mountains is constructed from the development of the late Cenozoic alluvial fan around Daluo Mountains.The entire sedimentary sequence and framework of the fan was revealed by the newly obtained drilling core data.The cosmogenic nuclide,optically stimulated luminescence,and detrital zircon U-Pb dating results provide new evidences for discussion about the initial timing of the late Cenozoic uplift of Daluo Mountains and the key stages of uplift during the Pleistocene.The late Cenozoic alluvial fan at front of Daluo Mountains overlies a set of fluvial-facies strata;therefore,development of the alluvial-fan marks the start of late Cenozoic uplift of Daluo Mountains.The timing of this event can be constrained to~4.64 Ma.Two extensive gravel layers(dated to ca.0.76–0.6 Ma and~0.05 Ma)developed during the Pleistocene,indicating two episodes of considerable uplift.This study provides a new time scale for the uplift and expansion of the arcuate tectonic belt at the northeastern margin of the Tibetan Plateau.展开更多
Construction planning of eco-cemeteries in Daluo Mountain,Wenzhou City was taken for example to explore application of ecological theories and principles in the planning of cemetery,which improves the cemetery plannin...Construction planning of eco-cemeteries in Daluo Mountain,Wenzhou City was taken for example to explore application of ecological theories and principles in the planning of cemetery,which improves the cemetery planning to the level of ecological construction.We arranged the construction of eco-cemeteries in Daluo Mountain in a unified layout through exploring approaches suitable for the present development stage to comply with actual conditions and facilitate the future management,and also prepare ideological guidance and material foundation for the construction of the cemetery.展开更多
Kuo,Yeh-Tzu founded Taiwan’s Sung Shan Tsu Huei Temple in 1970.She organized more than 200 worshipers as a group named“Taiwan Tsu Huei Temple Queen Mother of the West Delegation to China to Worship at the Ancestral ...Kuo,Yeh-Tzu founded Taiwan’s Sung Shan Tsu Huei Temple in 1970.She organized more than 200 worshipers as a group named“Taiwan Tsu Huei Temple Queen Mother of the West Delegation to China to Worship at the Ancestral Temples”in 1990.At that time,the temple building of the Queen Mother Palace in Huishan of Gansu Province was in disrepair,and Temple Master Kuo,Yeh-Tzu made a vow to rebuild it.Rebuilding the ancestral temple began in 1992 and was completed in 1994.It was the first case of a Taiwan temple financing the rebuilding of a far-away Queen Mother Palace with its own donations.In addition,Sung Shan Tsu Huei Temple celebrated its 45th anniversary and hosted Yiwei Yuanheng Lizhen Daluo Tiandi Qingjiao(Momentous and Fortuitous Heaven and Earth Prayer Ceremony)in 2015.This is the most important and the grandest blessing ceremony of Taoism,a rare event for Taoism locally and abroad during this century.Those sacred rituals were replete with unprecedented grand wishes to propagate the belief in Queen Mother of the West.Stopping at nothing,Queen Mother’s love never ceases.展开更多
目的:研究益肾达络饮对复发-缓解型EAE小鼠发病率、发病时间、复发率、神经功能评分及病理特征的影响。方法:用HE染色和Luxol fast blue染色观察EAE小鼠的病理特征。结果:中药组EAE小鼠的复发率(14.27%)低于模型组(62.5%);免疫后第21天...目的:研究益肾达络饮对复发-缓解型EAE小鼠发病率、发病时间、复发率、神经功能评分及病理特征的影响。方法:用HE染色和Luxol fast blue染色观察EAE小鼠的病理特征。结果:中药组EAE小鼠的复发率(14.27%)低于模型组(62.5%);免疫后第21天神经功能评分,中药组(1.86±0.40)低于模型组(3.13±0.69);免疫后第61天神经功能评分,中药组(0.86±0.55)低于模型组(2.75±0.73),差异有统计学意义(P<0.05)。结论:益肾达络饮能降低EAE小鼠的复发率、神经功能评分,减轻炎性细胞浸润和髓鞘脱失,但其作用机制需要进一步研究。展开更多
基金This work was supported by the Innovation Team and Talents Cultivation Program of National Administration of Traditional Chi-nese Medicine(ZYYCXTD-C-202006).
文摘Objective:To explore the mechanism and related active components of Yishen Daluo decoction(YSDLD)in treating multiple sclerosis(MS).Methods:Targets of YSDLD were collected through the TCMSP,Chemistry,and TCMID databases.The MS targets were collected through OMIM,DrugBank,Gencards,TTD,and Pharmgkb databases.We built“componentetarget”network diagrams and proteineprotein interaction(PPI)diagrams and performed topological analysis.The targets were subjected to GO and KEGG enrichment analysis.Molecular docking verification was conducted on selected targets and molecules.Finally,in vitro experiments were con-ducted.BV2 cells were induced by lipopolysaccharide for model establishment.CCK8 experiment was conducted to explore the effect of YSDLD and RT-qPCR technology was used to explore the expression of key targets.Results:There were 184 active components in YSDLD and 898 targets of its action.There were 940 MS targets,and 215 targets were shared by YSDLD and MS.According to the“componentetarget”diagram,the top five key components included quercetin,kaempferol,beta-sitosterol,stigmasterol,and nar-ingenin.IL-6,IL-1 b,TNF-α,AKT1,and VEGFA were the important targets identified by PPI network to-pology analysis.A total of 564 functions were identified by GO enrichment analysis(P<0.01),mainly involving inflammatory response,hypoxia response,plasma membrane,neuronal cell body,protein phosphatase binding,and cytokine activity.KEGG enrichment analysis enriched 98 pathways(P<.01).YSDLD at the concentration of 20 m g/mL had no effect on BV2 cells.RT-qPCR indicated that YSDLD at the concentrations of 15 m g/mL and 20 m g/mL alleviated LPS-induced inflammatory injury and lowered the content of inflammatory factors(P<0.05).Conclusion:In this paper,the network pharmacology and in vitro experiments were used to explore the potential mechanism of YSDLD in treating MS.The research provides a good basis for the development of YSDLD and drugs for MS in future.
基金supported by the National Natural Science Foundation of China(41972119)the Foundation of Chinese Academy of Geological Sciences(DZLXJK202104)the Geological Investigation Project of the China Geological Survey(DD20160060,DD20190018).
文摘Daluo Mountains lie at front of the arcuate tectonic belt at the northeastern margin of the Tibetan Plateau,and are the landform boundary zone between the active Tibetan Plateau and the stable North China Craton.Studying of the late Cenozoic uplift evolution of Daluo Mountains is important for understanding the expansion mechanism of the northeastern margin of the Tibetan Plateau and its influence on the western North China Craton.In this study,the late Cenozoic uplift of Daluo Mountains is constructed from the development of the late Cenozoic alluvial fan around Daluo Mountains.The entire sedimentary sequence and framework of the fan was revealed by the newly obtained drilling core data.The cosmogenic nuclide,optically stimulated luminescence,and detrital zircon U-Pb dating results provide new evidences for discussion about the initial timing of the late Cenozoic uplift of Daluo Mountains and the key stages of uplift during the Pleistocene.The late Cenozoic alluvial fan at front of Daluo Mountains overlies a set of fluvial-facies strata;therefore,development of the alluvial-fan marks the start of late Cenozoic uplift of Daluo Mountains.The timing of this event can be constrained to~4.64 Ma.Two extensive gravel layers(dated to ca.0.76–0.6 Ma and~0.05 Ma)developed during the Pleistocene,indicating two episodes of considerable uplift.This study provides a new time scale for the uplift and expansion of the arcuate tectonic belt at the northeastern margin of the Tibetan Plateau.
文摘Construction planning of eco-cemeteries in Daluo Mountain,Wenzhou City was taken for example to explore application of ecological theories and principles in the planning of cemetery,which improves the cemetery planning to the level of ecological construction.We arranged the construction of eco-cemeteries in Daluo Mountain in a unified layout through exploring approaches suitable for the present development stage to comply with actual conditions and facilitate the future management,and also prepare ideological guidance and material foundation for the construction of the cemetery.
文摘Kuo,Yeh-Tzu founded Taiwan’s Sung Shan Tsu Huei Temple in 1970.She organized more than 200 worshipers as a group named“Taiwan Tsu Huei Temple Queen Mother of the West Delegation to China to Worship at the Ancestral Temples”in 1990.At that time,the temple building of the Queen Mother Palace in Huishan of Gansu Province was in disrepair,and Temple Master Kuo,Yeh-Tzu made a vow to rebuild it.Rebuilding the ancestral temple began in 1992 and was completed in 1994.It was the first case of a Taiwan temple financing the rebuilding of a far-away Queen Mother Palace with its own donations.In addition,Sung Shan Tsu Huei Temple celebrated its 45th anniversary and hosted Yiwei Yuanheng Lizhen Daluo Tiandi Qingjiao(Momentous and Fortuitous Heaven and Earth Prayer Ceremony)in 2015.This is the most important and the grandest blessing ceremony of Taoism,a rare event for Taoism locally and abroad during this century.Those sacred rituals were replete with unprecedented grand wishes to propagate the belief in Queen Mother of the West.Stopping at nothing,Queen Mother’s love never ceases.
文摘目的:研究益肾达络饮对复发-缓解型EAE小鼠发病率、发病时间、复发率、神经功能评分及病理特征的影响。方法:用HE染色和Luxol fast blue染色观察EAE小鼠的病理特征。结果:中药组EAE小鼠的复发率(14.27%)低于模型组(62.5%);免疫后第21天神经功能评分,中药组(1.86±0.40)低于模型组(3.13±0.69);免疫后第61天神经功能评分,中药组(0.86±0.55)低于模型组(2.75±0.73),差异有统计学意义(P<0.05)。结论:益肾达络饮能降低EAE小鼠的复发率、神经功能评分,减轻炎性细胞浸润和髓鞘脱失,但其作用机制需要进一步研究。
