Objective:To determine the therapeutic effect of Danggui Shaoyao San with Huangqi on rats with nephrotic syndrome(NS)was discussed,and the scientific connotation of TCM treatment principle was elaborated,so as to prov...Objective:To determine the therapeutic effect of Danggui Shaoyao San with Huangqi on rats with nephrotic syndrome(NS)was discussed,and the scientific connotation of TCM treatment principle was elaborated,so as to provide experimental basis for its clinical application.Methods:The NS rat model was established by iv administration of adriamycin;The rats were divided into model group,Danggui Shaoyao San group,Danggui Shaoyao San with Huangqi group,Huangqi group and positive drug group,with 10 rats in control group;The best dosage ratio of Danggui Shaoyao San and Huangqi was screened by Excel;24 h urine protein was measured by BCA method;The electrolyte level was detected by automatic biochemical analyzer;In serum Urea nitrogen(BUN),Creatinine(SCr),Total cholesterol(TC),Triglyceride(TG),Total protein(TP),Albumin(ALB),Immunoglobulin M(Ig-M)and Immunoglobulin G(Ig-G)were detected by the kit;hematoxylin-eosin staining was used to observe the renal morphology;The Total water content(TBW),Extracellular water content(ECF)and Intracellular water content(ICF)were measured by experimental animal body composition analyzer.Results:Compared with the model group,the urine protein in the modified Astragalus group of Danggui Shaoyao Powder was significantly decreased(P<0.01);Urine electrolytes were significantly decreased and serum electrolytes were significantly increased(P<0.01);Serum BUN,SCR,TC and TG were significantly decreased(P<0.01),while TP,ALB,TBW and ICF were significantly increased(P<0.01);The levels of Ig-M and Ig-G were significantly increased(P<0.01),TBW and ICF were significantly increased,and ECF was significantly decreased(P<0.01);Histopathology showed that the pathological changes of renal tissue were obviously alleviated;The improvement of each index was better than that of Danggui Shaoyao San group and Huangqi group.Conclusion:Danggui Shaoyao San with Huangqi group can significantly improve the edema,proteinuria,blood lipid and immune function of NS rats,and the effect is better than that of Danggui Shaoyao San group and Huangqi group.It has the best therapeutic effect on NS,which indicates that Huangqi plays a key synergistic effect on Danggui Shaoyao San,which verifies the scientific connotation of"Yiqi Huoxue Lishui"in the treatment of NS.展开更多
目的研究当归芍药散(Danggui Shaoyao San,DSS)对糖尿病肾病小鼠肠道菌群的作用。方法选取30只8周龄雄性db/db小鼠,6只8周龄雄性db/m小鼠。检测空腹血糖以及尿白蛋白/肌酐比值(urinary albumin to creatinine ratio,UACR),将符合要求的d...目的研究当归芍药散(Danggui Shaoyao San,DSS)对糖尿病肾病小鼠肠道菌群的作用。方法选取30只8周龄雄性db/db小鼠,6只8周龄雄性db/m小鼠。检测空腹血糖以及尿白蛋白/肌酐比值(urinary albumin to creatinine ratio,UACR),将符合要求的db/db小鼠随机分成5组:模型(MOD)组(0.1 mL/10 g蒸馏水)、达格列净(DAPA)组(1.3 mg/kg达格列净片)及DSS低剂量(DSS-L)组(8.39 g/kg)、中剂量(DSS-M)组(16.77 g/kg)、高剂量(DSS-H)组(33.54 g/kg),每组6只;6只db/m小鼠为空白对照(CON)组(0.1 m L/10 g蒸馏水)。测定小鼠空腹血糖、胰岛素耐量;ELISA法测定血尿素氮和血肌酐;全自动生化分析仪检测UACR;对小鼠的肾脏组织和小肠组织进行HE染色;取小鼠结肠粪便进行16S r RNA测序。结果与CON组比较,MOD组糖耐量下降,空腹血糖、血尿素氮、血肌酐、UACR升高(P<0.01);与MOD组比较,DSS组的糖耐量升高(P<0.01),空腹血糖、血尿素氮、血肌酐、UACR降低(P<0.01),肾脏和小肠的病理损伤程度降低。肠道菌群多样性研究结果显示:相比CON组,MOD组肠道菌群多样性降低;相比MOD组,DSS-M组与CON组的菌群差异性较小。在菌群的物种组成方面,DSS-M组小鼠嗜黏蛋白阿克曼氏菌(Akkermansia muciniphila)的相对丰度明显增加(P<0.01)。结论DSS能显著增加糖尿病肾病db/db小鼠肠道中的嗜黏蛋白阿克曼氏菌的丰度,可能是其改善糖尿病肾病的机制之一。展开更多
目的 探讨当归芍药散(DSS)含药血清及其有效成分阿魏酸(FA)、芍药苷(PF)对肾间质纤维化(RIF)大鼠的作用及机制。方法 制备当归芍药散药液,以低剂量(0.05 mL DSS药液/10 g体质量)、中剂量(0.1 mL DSS药液/10 g体质量)、高剂量(0.2 mL DS...目的 探讨当归芍药散(DSS)含药血清及其有效成分阿魏酸(FA)、芍药苷(PF)对肾间质纤维化(RIF)大鼠的作用及机制。方法 制备当归芍药散药液,以低剂量(0.05 mL DSS药液/10 g体质量)、中剂量(0.1 mL DSS药液/10 g体质量)、高剂量(0.2 mL DSS药液/10 g体质量)每日同一时间灌胃雄性Sprague Dawley大鼠,持续7 d,腹主动脉取血获得相应低、中、高剂量DSS血清(DSSS)。将大鼠肾小管上皮细胞(NRK-52E)分成对照组、模型组(TGF-β处理)、siRNA转染组(TGF-β+siRNA处理)、FA组(TGF-β+FA处理)、PF组(TGF-β+PF处理)及DSSS低、中、高剂量组(TGF-β+DSSS-L、TGF-β+DSSS-M、TGF-β+DSSS-H分别处理)。筛选并确定DSSS、FA、PF、TGF-β对NRK-52E细胞的干预浓度,对以上各组NRK-52E细胞进行干预。采用Western blotting法检测各组NLRP3、半胱氨酸蛋白酶1(CASP-1)、Gasdermin D(GSDMD)、白细胞介素-18(IL-18)、纤连蛋白(FN)、E型钙黏蛋白(E-Cadherin)、N型钙黏蛋白(N-Cadherin)的蛋白表达变化。结果 相较于模型组,DSSS、FA、PF治疗组细胞焦亡相关蛋白NLRP3、CASP-1、GSDMD、IL-18表达均降低,差异均有统计学意义(P均<0.05)。DSSS中、高剂量及FA、PF治疗组相较于模型组纤维化相关黏附蛋白FN、E-Cadherin、N-Cadherin表达降低,差异均有统计学意义(P均<0.05)。结论 当归芍药散含药血清及有效成分FA、PF可抑制TGF-β诱导的NRK-52E细胞纤维化模型中NLRP3介导的焦亡蛋白激活,并能调节黏附蛋白表达和上皮-间质转化进展,缓解纤维化。展开更多
Background： The pain caused by orthodontic treatment has been considered as tough problems in orthodontic practice. There is substantial literature on pain which has exactly effected on learning and memory; orthodont...Background： The pain caused by orthodontic treatment has been considered as tough problems in orthodontic practice. There is substantial literature on pain which has exactly effected on learning and memory; orthodontic tooth movement affected the emotional status has been showed positive outcomes. Danggui-Shaoyao-San （DSS） is a Traditional Chinese Medicine prescription that has been used for pain treatment and analgesic effect for orthodontic pain via inhibiting the activations of neuron and glia. We raised the hypothesis that DSS could restore the impaired abilities of spatial learning and memory via regulating neuron or glia expression in the hippocampus. Methods： A total of 36 rats were randornly divided into three groups： （ 1 ） Sham group （n = 12）, rats underwent all the operation procedure except for the placement of orthodontic forces and received saline treatment： （2） experimental tooth movement （ETM） group （n - 12）, rats received saline treatment and ETM： （3） DSS ＋ ETM （DETM） group （n = 12）, rats received DSS treatment and ETM. All DETM group animals were administered with DSS at a dose of 150 mg/kg. Monis water maze test was evaluated： immunofluorescent histochemistry was used to identity astrocytes activation, and immunofluorescent dendritic spine analysis was used to identify the dendritic spines morphological characteristics expression levels in hippocampus. Results： Maze training sessions during the 5 successive days revealed that ETM significantly deficits in progressive learning in rats, DSS that was given from day 5 prior to ETM enhanced progressive learning. The ETM group rats took longer to cross target quadrant during the probe trial and got less times to cross-platform than DETM group. The spine density in hippocampus in ETM group was significantly decreased cornpared to the sham group. In addition, thin and mature spine density were decreased too. However, the DSS administration could reverse the dendritic shrinkage and increase the spine density compared to the ETM group. Astrocytes activation showed the opposite trend in hippocampus dentate gyrus （DG）. Conclusions： Treatment with DSS could restore the impaired abilities on ETM-induced decrease of learning and memory behavior. The decreased spines density in the hippocampus and astrocytes activation in DG ofhippocampus in the ETM group rats may be related with the decline of the ability of learning and rnernory. The ability to change the synaptic plasticity in hippocarnpus after DSS administration may be correlated with the alleviation of impairment of learn and memory atter ETM treatment.展开更多
Background The pain caused by orthodontic treatment has been considered as tough problems in orthodontic practice. Danggui-shaoyao-san （DSS） is a traditional Chinese medicine （TCM） prescription which has long been...Background The pain caused by orthodontic treatment has been considered as tough problems in orthodontic practice. Danggui-shaoyao-san （DSS） is a traditional Chinese medicine （TCM） prescription which has long been used for pain treatment and possesses antioxidative, cognitive enhancing and antidepressant effects. We raise the hypothesis that DSS exerts analgesic effect for orthodontic pain via inhibiting the activations of neuron and microglia. Methods DSS was given twice a day from day 5 prior to experimental tooth movement （ETM）. Directed face grooming and vacuous chewing movements （VCM） were evaluated. Immunofiuorescent histochemistry and Western blot analysis were used to quantify the Iba-1 （microglia activation） and Fos （neuronal activation） expression levels in the trigeminal spinal nucleus caudalis （Vc）. Results ETM significantly increased directed face grooming and VCM which reached the peak at post-operative day （POD） 1 and gradually decreased to the baseline at POD 7. However, a drastic peak increase of Fos expression in Vc was observed at 4 hours and gradually decreased to baseline at POD 7; while the increased Iba-1 level reached the peak at POD 1 and gradually decreased to baseline at POD 7. Furthermore, pre-treatment with DSS significantly attenuated the ETM induced directed face grooming and VCM as well as the Fos and Iba-1 levels at POD 1. Conclusion Treatment with DSS had significant analgesic effects on ETM-induced pain, which was accompanied with inhibition of both neuronal and microglial activation.展开更多
基金National Natural Science Foundation Project(No.81573720,81974536)。
文摘Objective:To determine the therapeutic effect of Danggui Shaoyao San with Huangqi on rats with nephrotic syndrome(NS)was discussed,and the scientific connotation of TCM treatment principle was elaborated,so as to provide experimental basis for its clinical application.Methods:The NS rat model was established by iv administration of adriamycin;The rats were divided into model group,Danggui Shaoyao San group,Danggui Shaoyao San with Huangqi group,Huangqi group and positive drug group,with 10 rats in control group;The best dosage ratio of Danggui Shaoyao San and Huangqi was screened by Excel;24 h urine protein was measured by BCA method;The electrolyte level was detected by automatic biochemical analyzer;In serum Urea nitrogen(BUN),Creatinine(SCr),Total cholesterol(TC),Triglyceride(TG),Total protein(TP),Albumin(ALB),Immunoglobulin M(Ig-M)and Immunoglobulin G(Ig-G)were detected by the kit;hematoxylin-eosin staining was used to observe the renal morphology;The Total water content(TBW),Extracellular water content(ECF)and Intracellular water content(ICF)were measured by experimental animal body composition analyzer.Results:Compared with the model group,the urine protein in the modified Astragalus group of Danggui Shaoyao Powder was significantly decreased(P<0.01);Urine electrolytes were significantly decreased and serum electrolytes were significantly increased(P<0.01);Serum BUN,SCR,TC and TG were significantly decreased(P<0.01),while TP,ALB,TBW and ICF were significantly increased(P<0.01);The levels of Ig-M and Ig-G were significantly increased(P<0.01),TBW and ICF were significantly increased,and ECF was significantly decreased(P<0.01);Histopathology showed that the pathological changes of renal tissue were obviously alleviated;The improvement of each index was better than that of Danggui Shaoyao San group and Huangqi group.Conclusion:Danggui Shaoyao San with Huangqi group can significantly improve the edema,proteinuria,blood lipid and immune function of NS rats,and the effect is better than that of Danggui Shaoyao San group and Huangqi group.It has the best therapeutic effect on NS,which indicates that Huangqi plays a key synergistic effect on Danggui Shaoyao San,which verifies the scientific connotation of"Yiqi Huoxue Lishui"in the treatment of NS.
文摘目的研究当归芍药散(Danggui Shaoyao San,DSS)对糖尿病肾病小鼠肠道菌群的作用。方法选取30只8周龄雄性db/db小鼠,6只8周龄雄性db/m小鼠。检测空腹血糖以及尿白蛋白/肌酐比值(urinary albumin to creatinine ratio,UACR),将符合要求的db/db小鼠随机分成5组:模型(MOD)组(0.1 mL/10 g蒸馏水)、达格列净(DAPA)组(1.3 mg/kg达格列净片)及DSS低剂量(DSS-L)组(8.39 g/kg)、中剂量(DSS-M)组(16.77 g/kg)、高剂量(DSS-H)组(33.54 g/kg),每组6只;6只db/m小鼠为空白对照(CON)组(0.1 m L/10 g蒸馏水)。测定小鼠空腹血糖、胰岛素耐量;ELISA法测定血尿素氮和血肌酐;全自动生化分析仪检测UACR;对小鼠的肾脏组织和小肠组织进行HE染色;取小鼠结肠粪便进行16S r RNA测序。结果与CON组比较,MOD组糖耐量下降,空腹血糖、血尿素氮、血肌酐、UACR升高(P<0.01);与MOD组比较,DSS组的糖耐量升高(P<0.01),空腹血糖、血尿素氮、血肌酐、UACR降低(P<0.01),肾脏和小肠的病理损伤程度降低。肠道菌群多样性研究结果显示:相比CON组,MOD组肠道菌群多样性降低;相比MOD组,DSS-M组与CON组的菌群差异性较小。在菌群的物种组成方面,DSS-M组小鼠嗜黏蛋白阿克曼氏菌(Akkermansia muciniphila)的相对丰度明显增加(P<0.01)。结论DSS能显著增加糖尿病肾病db/db小鼠肠道中的嗜黏蛋白阿克曼氏菌的丰度,可能是其改善糖尿病肾病的机制之一。
文摘Background： The pain caused by orthodontic treatment has been considered as tough problems in orthodontic practice. There is substantial literature on pain which has exactly effected on learning and memory; orthodontic tooth movement affected the emotional status has been showed positive outcomes. Danggui-Shaoyao-San （DSS） is a Traditional Chinese Medicine prescription that has been used for pain treatment and analgesic effect for orthodontic pain via inhibiting the activations of neuron and glia. We raised the hypothesis that DSS could restore the impaired abilities of spatial learning and memory via regulating neuron or glia expression in the hippocampus. Methods： A total of 36 rats were randornly divided into three groups： （ 1 ） Sham group （n = 12）, rats underwent all the operation procedure except for the placement of orthodontic forces and received saline treatment： （2） experimental tooth movement （ETM） group （n - 12）, rats received saline treatment and ETM： （3） DSS ＋ ETM （DETM） group （n = 12）, rats received DSS treatment and ETM. All DETM group animals were administered with DSS at a dose of 150 mg/kg. Monis water maze test was evaluated： immunofluorescent histochemistry was used to identity astrocytes activation, and immunofluorescent dendritic spine analysis was used to identify the dendritic spines morphological characteristics expression levels in hippocampus. Results： Maze training sessions during the 5 successive days revealed that ETM significantly deficits in progressive learning in rats, DSS that was given from day 5 prior to ETM enhanced progressive learning. The ETM group rats took longer to cross target quadrant during the probe trial and got less times to cross-platform than DETM group. The spine density in hippocampus in ETM group was significantly decreased cornpared to the sham group. In addition, thin and mature spine density were decreased too. However, the DSS administration could reverse the dendritic shrinkage and increase the spine density compared to the ETM group. Astrocytes activation showed the opposite trend in hippocampus dentate gyrus （DG）. Conclusions： Treatment with DSS could restore the impaired abilities on ETM-induced decrease of learning and memory behavior. The decreased spines density in the hippocampus and astrocytes activation in DG ofhippocampus in the ETM group rats may be related with the decline of the ability of learning and rnernory. The ability to change the synaptic plasticity in hippocarnpus after DSS administration may be correlated with the alleviation of impairment of learn and memory atter ETM treatment.
基金This work was supported by grants from the National Natural Science Foundation of China （No. 30772450, No. 81170929 and No. 30873322）, the Open Project Program of State Key Laboratory of Natural Medicines, China Pharmaceutical University （No. SKLNMKF201214）. Conflict of interest： none.
文摘Background The pain caused by orthodontic treatment has been considered as tough problems in orthodontic practice. Danggui-shaoyao-san （DSS） is a traditional Chinese medicine （TCM） prescription which has long been used for pain treatment and possesses antioxidative, cognitive enhancing and antidepressant effects. We raise the hypothesis that DSS exerts analgesic effect for orthodontic pain via inhibiting the activations of neuron and microglia. Methods DSS was given twice a day from day 5 prior to experimental tooth movement （ETM）. Directed face grooming and vacuous chewing movements （VCM） were evaluated. Immunofiuorescent histochemistry and Western blot analysis were used to quantify the Iba-1 （microglia activation） and Fos （neuronal activation） expression levels in the trigeminal spinal nucleus caudalis （Vc）. Results ETM significantly increased directed face grooming and VCM which reached the peak at post-operative day （POD） 1 and gradually decreased to the baseline at POD 7. However, a drastic peak increase of Fos expression in Vc was observed at 4 hours and gradually decreased to baseline at POD 7; while the increased Iba-1 level reached the peak at POD 1 and gradually decreased to baseline at POD 7. Furthermore, pre-treatment with DSS significantly attenuated the ETM induced directed face grooming and VCM as well as the Fos and Iba-1 levels at POD 1. Conclusion Treatment with DSS had significant analgesic effects on ETM-induced pain, which was accompanied with inhibition of both neuronal and microglial activation.
