A new daphnane diterpene was isolated from the root barks of Daphne tangutica Maxim. Its structure was elucidated as 1, 2α-dihydro-20-palimoyldaphnctoxin by the spectroscopic evidence including 2D-NMR.
Background: Daphne Mucronata extract has a decreasing effect on the size of breast adenocarcinoma in rats. So in this study, the effect of Daphne Mucronata purified diterpene were investigated on co culture of human m...Background: Daphne Mucronata extract has a decreasing effect on the size of breast adenocarcinoma in rats. So in this study, the effect of Daphne Mucronata purified diterpene were investigated on co culture of human monocytes and two human leukemia cell lines (K562, CCRF-CEM). Materials and Methods: Each cell line mono-layer culture, in log phase growth, was treated with 10 to 160 μL of the extract (1 g/ml leave powder) and purified compound (0.94 nM). For a comparative study, Taxol (5 to 40 μM) was used in the presence and absence of LPS. Human monocytes were isolated by adhesion method. TNF-α in cultured media were measured by sensitive biotin-streptoavidin ELISA method. Results: Fifty percent of growth inhibition was shown by 160 μL (1:100 dilution, 0.5 g of the powdered leaves/ml) of the extract and 0.94 nM of the purified component, and there was more inhibition in K562 cells (P < 0.05). Four fold increases in growth inhibition was shown in co culture of isolated human monocytes and leukemia cell lines. There was a direct relationship between monocytes TNF-α secretion and growth inhibition degree. Conclusion: Daphne Mucronata extract and its purified diterpene through increasing monocytes TNF-α releasing, potentially inhibit Leukemia cell line.展开更多
Objective: To investigate the analgesic and anti-inflammatory property and possible involvement of opioid receptors of ethyl acetate extract from aerial parts of Daphne mucronata(D. mucronata) in mice by formalin test...Objective: To investigate the analgesic and anti-inflammatory property and possible involvement of opioid receptors of ethyl acetate extract from aerial parts of Daphne mucronata(D. mucronata) in mice by formalin test.Methods: Single doses of 2.5, 5.0 and 10.0 mg/kg of body weight of ethyl acetate extract of D. mucronata were intraperitoneally administered to the mice 30 min before analgesic test. The anti-nociceptive effect of preparations was evaluated based on the formalin in mice.Results: The results indicated that the extract(2.5, 5.0 and 10.0 mg/kg) increased the pain threshold of mice and induced analgesia in both phases of formalin test. Like morphine sulfate(5.0 mg/kg, i.p.), the extract also showed more effective analgesic effect on the late phase of formalin test. Pre-treatment of animals with naloxone(5.0 mg/kg i.p.)did not inhibit the effects of the extract.Conclusions: Our findings suggest that D. mucronata contains potential analgesic and anti-inflammatory compounds which support its traditional use. Moreover, it seems that the analgesic and anti-inflammatory effects of the extract is mediated by non-opioid mechanisms. Further pharmacological studies are required to determine whether the analgesic mechanisms are actually responsible for such properties.展开更多
Prochloraz diluents( 450 ×,475 ×,500 ×) were added with different concentrations of nano-titanium dioxide( Ti O2)( 0. 8%,1. 0%) respectively to prepare complex fresh-keeping agents,which were applied in...Prochloraz diluents( 450 ×,475 ×,500 ×) were added with different concentrations of nano-titanium dioxide( Ti O2)( 0. 8%,1. 0%) respectively to prepare complex fresh-keeping agents,which were applied in the refrigeration of Dioscorea alata Lirm. sp. With rot index,weight loss rate,browning degree( BD),total soluble solids( TSS) content,malondialdehyde( MDA) content,polyphenol oxidase( PPO) activity and peroxidase( POD) activity,DPPH· scavenging rate as evaluation indexes,the preservation effect of the prepared freshkeeping agents on D. alata during the cold storage period was analyzed. The results showed that prochloraz added with nano-titanium dioxide significantly prolonged the storage life,reduced the rot rate and weight loss rate,and slowed the loss of pulp of D. alata. The preservation effect of complex fresh-keeping agent of prochloraz diluent( 475 ×) and nano-titanium dioxide( 1. 0%) was better than that of other treatments.展开更多
A new compound, named daphnolin （1）, was isolated from the stem barks of Daphne giraldii Nitsche. Its structure was elucidated as 8-hydroxy-7-O-β-D-glucopyranosyl-5-（2-oxo-2H-1-benzopyran-7-hydroxy-8-yloxy）-2H-1-...A new compound, named daphnolin （1）, was isolated from the stem barks of Daphne giraldii Nitsche. Its structure was elucidated as 8-hydroxy-7-O-β-D-glucopyranosyl-5-（2-oxo-2H-1-benzopyran-7-hydroxy-8-yloxy）-2H-1-benzopyran-2-one by means of NMR and MS analysis.展开更多
Three compounds were isolated from the tissue culture cells of Daphne giraldii cullus, their structures were identified as daphneolone （1）, S-（＋）-1-（4-hydroxy-3-methoxyphenyl）-3-hydroxy-5-phenyl-l-pentanone （2...Three compounds were isolated from the tissue culture cells of Daphne giraldii cullus, their structures were identified as daphneolone （1）, S-（＋）-1-（4-hydroxy-3-methoxyphenyl）-3-hydroxy-5-phenyl-l-pentanone （2）, S-（＋）-1-（4-methoxyphenyl）-3- hydroxy-5-phenyl-l-pentanone （3）, and among them, 2 was a new compound, 3 was a novel natural product.展开更多
A new coumarin glycoside was isolated from the ethanol extract of the barks of stem of Daphne giraldii Nitsche. Its structure was defined as daphnetin 8-O-β-D-glucopyranosyl-（1 → 6）-β-D-glucopyranoside on the bas...A new coumarin glycoside was isolated from the ethanol extract of the barks of stem of Daphne giraldii Nitsche. Its structure was defined as daphnetin 8-O-β-D-glucopyranosyl-（1 → 6）-β-D-glucopyranoside on the basis of spectral evidences.展开更多
Daphne plants,belonging to Thymelaeaceae,have been used as traditional Chinese medicine for thousands of years.Previous studies suggested that they exerted anti-tumor activities and reported a number of compounds with...Daphne plants,belonging to Thymelaeaceae,have been used as traditional Chinese medicine for thousands of years.Previous studies suggested that they exerted anti-tumor activities and reported a number of compounds with anti-tumor activities,isolated from the genus Daphne.However,the targets of these compounds have not been determined yet.In this study,network pharmacology was used to analyze 128 terpenoid compounds from the genus Daphne and 49 target proteins of liver cancer.The results suggested that the target with the most number of directed edges was 5yz0.The compound which docked with 5yz0 best is AR.DT.25(acutilobin D),and the compounds with total docking score greater than 10 were almost all Daphnane diterpenoids.The network pharmacology results predicted that the 5yz0 protein was one of the potential targets of the terpenoids from the genus Daphne and then suggested several new targets and compounds for further liver cancer research.展开更多
Plant of Daphne odora Thunb.has been used as medicinal plant since ancient times.In recent years,phytochemical studies have found that D.odora contains flavonoids,diflavonoids,coumarins,lignans,monoterpenes and other ...Plant of Daphne odora Thunb.has been used as medicinal plant since ancient times.In recent years,phytochemical studies have found that D.odora contains flavonoids,diflavonoids,coumarins,lignans,monoterpenes and other compounds,and shows extensive biological activities such as anti-inflammatory,immunomodulatory,neuroinhibition effects and so on.This article briefly reviews the recent research progress on the chemical constituents and pharmacological effects of D.odora at home and abroad.展开更多
One day Cupid, the little god of love, sat on thebank of a river, playing with his arrows. The arrowswere very tiny. Some had points of gold, and others hadpoints of lead. None of them looked as if they could domuch h...One day Cupid, the little god of love, sat on thebank of a river, playing with his arrows. The arrowswere very tiny. Some had points of gold, and others hadpoints of lead. None of them looked as if they could domuch harm. That day Apollo, the great sun-god, walked along thebank of the same river, when returning from his展开更多
Daphne acutiloba Rehd., belonging to Daphne genus of Thymelaeaceae is an evergreen shrub mainly distributed in the west of China. The roots, stem barks, leaves and fruits of this plant had been used as folk medicines ...Daphne acutiloba Rehd., belonging to Daphne genus of Thymelaeaceae is an evergreen shrub mainly distributed in the west of China. The roots, stem barks, leaves and fruits of this plant had been used as folk medicines for the treatment of rheumatoid arthriths, apoplesia and stomachache ache. Daphne diterpenes and tieranes diterpenes, guaiacane type sesquiterpene, lignans, diflavane and flavonoids, sterides, aliphatics and phenols were isolated from Daphne acutiloba Rehd. Researches demonstrated that the main chemical constituents of D. acutiloba Rehd. have multiple activities and good development prospect. This paper is presented as a review centering on the main chemical constituents and pharmacological effects of D. acutiloba Rehd. for the further research and development of this plant.展开更多
文摘A new daphnane diterpene was isolated from the root barks of Daphne tangutica Maxim. Its structure was elucidated as 1, 2α-dihydro-20-palimoyldaphnctoxin by the spectroscopic evidence including 2D-NMR.
文摘Background: Daphne Mucronata extract has a decreasing effect on the size of breast adenocarcinoma in rats. So in this study, the effect of Daphne Mucronata purified diterpene were investigated on co culture of human monocytes and two human leukemia cell lines (K562, CCRF-CEM). Materials and Methods: Each cell line mono-layer culture, in log phase growth, was treated with 10 to 160 μL of the extract (1 g/ml leave powder) and purified compound (0.94 nM). For a comparative study, Taxol (5 to 40 μM) was used in the presence and absence of LPS. Human monocytes were isolated by adhesion method. TNF-α in cultured media were measured by sensitive biotin-streptoavidin ELISA method. Results: Fifty percent of growth inhibition was shown by 160 μL (1:100 dilution, 0.5 g of the powdered leaves/ml) of the extract and 0.94 nM of the purified component, and there was more inhibition in K562 cells (P < 0.05). Four fold increases in growth inhibition was shown in co culture of isolated human monocytes and leukemia cell lines. There was a direct relationship between monocytes TNF-α secretion and growth inhibition degree. Conclusion: Daphne Mucronata extract and its purified diterpene through increasing monocytes TNF-α releasing, potentially inhibit Leukemia cell line.
基金supported by a Pharmaceutical Sciences Branch, Islamic Azad University (IAUPS) Grant
文摘Objective: To investigate the analgesic and anti-inflammatory property and possible involvement of opioid receptors of ethyl acetate extract from aerial parts of Daphne mucronata(D. mucronata) in mice by formalin test.Methods: Single doses of 2.5, 5.0 and 10.0 mg/kg of body weight of ethyl acetate extract of D. mucronata were intraperitoneally administered to the mice 30 min before analgesic test. The anti-nociceptive effect of preparations was evaluated based on the formalin in mice.Results: The results indicated that the extract(2.5, 5.0 and 10.0 mg/kg) increased the pain threshold of mice and induced analgesia in both phases of formalin test. Like morphine sulfate(5.0 mg/kg, i.p.), the extract also showed more effective analgesic effect on the late phase of formalin test. Pre-treatment of animals with naloxone(5.0 mg/kg i.p.)did not inhibit the effects of the extract.Conclusions: Our findings suggest that D. mucronata contains potential analgesic and anti-inflammatory compounds which support its traditional use. Moreover, it seems that the analgesic and anti-inflammatory effects of the extract is mediated by non-opioid mechanisms. Further pharmacological studies are required to determine whether the analgesic mechanisms are actually responsible for such properties.
基金Supported by Natural Science Foundation of Guangxi(2017GXNSFAA198082)Guangxi First-class Discipline Cultivation Project for Food Science and Engineering(GXYLXP1812)
文摘Prochloraz diluents( 450 ×,475 ×,500 ×) were added with different concentrations of nano-titanium dioxide( Ti O2)( 0. 8%,1. 0%) respectively to prepare complex fresh-keeping agents,which were applied in the refrigeration of Dioscorea alata Lirm. sp. With rot index,weight loss rate,browning degree( BD),total soluble solids( TSS) content,malondialdehyde( MDA) content,polyphenol oxidase( PPO) activity and peroxidase( POD) activity,DPPH· scavenging rate as evaluation indexes,the preservation effect of the prepared freshkeeping agents on D. alata during the cold storage period was analyzed. The results showed that prochloraz added with nano-titanium dioxide significantly prolonged the storage life,reduced the rot rate and weight loss rate,and slowed the loss of pulp of D. alata. The preservation effect of complex fresh-keeping agent of prochloraz diluent( 475 ×) and nano-titanium dioxide( 1. 0%) was better than that of other treatments.
文摘A new compound, named daphnolin （1）, was isolated from the stem barks of Daphne giraldii Nitsche. Its structure was elucidated as 8-hydroxy-7-O-β-D-glucopyranosyl-5-（2-oxo-2H-1-benzopyran-7-hydroxy-8-yloxy）-2H-1-benzopyran-2-one by means of NMR and MS analysis.
文摘Three compounds were isolated from the tissue culture cells of Daphne giraldii cullus, their structures were identified as daphneolone （1）, S-（＋）-1-（4-hydroxy-3-methoxyphenyl）-3-hydroxy-5-phenyl-l-pentanone （2）, S-（＋）-1-（4-methoxyphenyl）-3- hydroxy-5-phenyl-l-pentanone （3）, and among them, 2 was a new compound, 3 was a novel natural product.
文摘A new coumarin glycoside was isolated from the ethanol extract of the barks of stem of Daphne giraldii Nitsche. Its structure was defined as daphnetin 8-O-β-D-glucopyranosyl-（1 → 6）-β-D-glucopyranoside on the basis of spectral evidences.
文摘Daphne plants,belonging to Thymelaeaceae,have been used as traditional Chinese medicine for thousands of years.Previous studies suggested that they exerted anti-tumor activities and reported a number of compounds with anti-tumor activities,isolated from the genus Daphne.However,the targets of these compounds have not been determined yet.In this study,network pharmacology was used to analyze 128 terpenoid compounds from the genus Daphne and 49 target proteins of liver cancer.The results suggested that the target with the most number of directed edges was 5yz0.The compound which docked with 5yz0 best is AR.DT.25(acutilobin D),and the compounds with total docking score greater than 10 were almost all Daphnane diterpenoids.The network pharmacology results predicted that the 5yz0 protein was one of the potential targets of the terpenoids from the genus Daphne and then suggested several new targets and compounds for further liver cancer research.
文摘Plant of Daphne odora Thunb.has been used as medicinal plant since ancient times.In recent years,phytochemical studies have found that D.odora contains flavonoids,diflavonoids,coumarins,lignans,monoterpenes and other compounds,and shows extensive biological activities such as anti-inflammatory,immunomodulatory,neuroinhibition effects and so on.This article briefly reviews the recent research progress on the chemical constituents and pharmacological effects of D.odora at home and abroad.
文摘One day Cupid, the little god of love, sat on thebank of a river, playing with his arrows. The arrowswere very tiny. Some had points of gold, and others hadpoints of lead. None of them looked as if they could domuch harm. That day Apollo, the great sun-god, walked along thebank of the same river, when returning from his
文摘Daphne acutiloba Rehd., belonging to Daphne genus of Thymelaeaceae is an evergreen shrub mainly distributed in the west of China. The roots, stem barks, leaves and fruits of this plant had been used as folk medicines for the treatment of rheumatoid arthriths, apoplesia and stomachache ache. Daphne diterpenes and tieranes diterpenes, guaiacane type sesquiterpene, lignans, diflavane and flavonoids, sterides, aliphatics and phenols were isolated from Daphne acutiloba Rehd. Researches demonstrated that the main chemical constituents of D. acutiloba Rehd. have multiple activities and good development prospect. This paper is presented as a review centering on the main chemical constituents and pharmacological effects of D. acutiloba Rehd. for the further research and development of this plant.
