A new coumarin glycoside from Daphne giraldii

A new coumarin glycoside was isolated from the ethanol extract of the barks of stem of Daphne giraldii Nitsche. Its structure was defined as daphnetin 8-O-β-D-glucopyranosyl-（1 → 6）-β-D-glucopyranoside on the basis of spectral evidences.

A new flavonoid glucoside from Cassia angustifolia

A new flavonoid glucoside with a known one was isolated from the leaves of Cassia angustifolia. Based on the spectral analysis, including MS, ^1H NMR, ^13C NMR, DEPT, ^1H-1HCOSY, HMQC, HMBC and NOSEY, their chemical structures were determined as kaempferol-3-O-[（6＇＇＇-O-trans-sinnapoyt）-β-D-glucopyranosyl （1 → 6）]-β-D-glucopyranoside 1 and apigenin-6,8-di-C-glycoside 2.

UPLC-Q/TOF法同时定性定量分析滇芹药材中主要化学成分

目的建立UPLC-Q/TOF法同时定性、定量分析滇芹中主要化学成分。方法采用超高效液相色谱串联飞行时间质谱法进行分析,色谱柱为Agilent Poroshell 120 Hilic,流动相为乙腈-0.1%甲酸水溶液,梯度洗脱,体积流量0.3 m L/min;定性、定量采用Q-TOF正负离子全扫描+IDA(信息关联采集)模式。结果利用已建立的筛查数据库将满足质量误差小于5×10-6、同位素分布正确且含有二级碎片的离子作为分析目标化合物,结合软件Formula Finder、Mass Calculators等功能、在线数据库(Human Metabolome Database、Pub Chem、Chemical Book等)及二级碎片裂解规律,共鉴定出23个化合物,滇芹首次发现4个化合物。确定阿魏酸为定量分析指标性成分,其定量、定性离子分别为178和149;阿魏酸检测质量浓度线性范围为1.14~1 140 ng/m L(r=1.000),检测限、定量限分别为0.87、2.91 ng/m L,精密度、稳定性、重复性试验的RSD<2%,加样回收率为98.13%~101.25%(RSD=1.37%,n=5)。结论该方法灵敏度高、重现性好,分析快速、准确、可靠,可用于同时定性、定量检测滇芹药材中主要化学成分;云南滇芹中阿魏酸含量要高于西藏滇芹。