Effects of Tremella Polysaccharide on Immune Function of Physically-Stressed Mice

An immunosuppressive animal model induced by physical stress that forced mice to swim in cold water(14±1℃)and the restorative effect of Tremella polysac charide(TP)on the suppressed immune function by stress were studied in mice.It was found that the spleen plaque forming cell(PFC)response to sheep red blood cells,delayed cuta- neous hypersensitivity(DCH)induced by dinitrochlorobenzene and the lymphocyte prolifer- ation stimulated by concanavalin A(Con A)were significantly decreased in stressed mice. In addition.the maximal decrease of PFC was reached in 9-12 days after stress.A- drenolectomy could not affect the decrease of PFC in stressed mice.TP(200.400mg/kg) ig for 8-14 days significantly restored the PFC.DCH and lymphocyte proliferation to nor- mal level in stressed mice.

异丙肌苷对小鼠免疫功能的增强作用

在正常小鼠和注射环磷酰胺所致的免疫功能抑制的小鼠,异丙肌苷)25,50,100mg/kg,ip)能显著促进溶血素生成;增加空斑形成细胞数;增强二硝基氯苯所致迟发型皮肤超敏反应。在16月龄老年小鼠,异丙肌苷(2.5mg/kg,ip)可使减少的空斑形成细胞数增加至接近3月龄小鼠的水平,加大剂量作用反减弱或使空斑形成细胞数显著减少。体外试验,异丙肌苷(5,10,20mg/L)可显著增强刀豆素A诱导的C57 BL/6J小鼠的淋巴细胞增殖反应,并使16月龄老年小鼠低下的刀豆素A诱导的淋巴细胞增殖反应明显恢复。