三七〔Panax nologinseng (Burk.) F. H. Chen〕为我国传统的名贵中药。近年来,几本学者小菅卓夫等对三七进行分离止血活性成分的研究,并分得止血活性最强的单体,命名为dencichine。又参照Betty的方法合成了该化合物,两者的理化性质及...三七〔Panax nologinseng (Burk.) F. H. Chen〕为我国传统的名贵中药。近年来,几本学者小菅卓夫等对三七进行分离止血活性成分的研究,并分得止血活性最强的单体,命名为dencichine。又参照Betty的方法合成了该化合物,两者的理化性质及生物试验结果完全一致。本文重点叙述denciehine和它的光学异构体的止血、增加血小板数和神经毒等作用。我所已于1984年合成了三七止血有效成分dencichine。展开更多
The enantioseparation of dencichine and its D-isomer was achieved on a novel chiral stationary phase via coating N-（2-hydroxyl-3-octoxyl） propyl-S-benzyl-（L）-cysteine on YWG-C18 phase by ligand exchange chromatogr...The enantioseparation of dencichine and its D-isomer was achieved on a novel chiral stationary phase via coating N-（2-hydroxyl-3-octoxyl） propyl-S-benzyl-（L）-cysteine on YWG-C18 phase by ligand exchange chromatography.展开更多
In order to quantitate dencichine in biological samples, a selective and sensitive method for the determination of dencichine in rat plasma based on high-performance liquid chromatography-tandem mass spectrometry (HPL...In order to quantitate dencichine in biological samples, a selective and sensitive method for the determination of dencichine in rat plasma based on high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) was developed and validated. (l)-2-amino-3-(carboxymethylthio)propionic acid was used as the internal standard (I.S.). After a protein precipitation extraction with acetonitrile, dencichine and the I.S. were chromatographed on an Xterra MS-C18 column. The mobile phase was consisted of 20mmol/L ammonium acetate solution-acetonitrile (35:65, V/V) at a flow rate of 0.2 mL/min. The detection was performed on a triple quadrupole mass via electrospray ionization (ESI) source in the positive mode. The intra- and inter-day precision (relative standard deviation, R.S.D.) values of dencichine were below 6.7%. The extraction recoveries were up 85%. The lower limit of quantification was 20 ng/ml, which was sensitive enough to detect the analyte. The HPLC-MS/MS method was successfully applied to the pharmacokinetic study after an intravenous administration of dencichine in Sprague-Dawley (SD) rat.展开更多
文摘三七〔Panax nologinseng (Burk.) F. H. Chen〕为我国传统的名贵中药。近年来,几本学者小菅卓夫等对三七进行分离止血活性成分的研究,并分得止血活性最强的单体,命名为dencichine。又参照Betty的方法合成了该化合物,两者的理化性质及生物试验结果完全一致。本文重点叙述denciehine和它的光学异构体的止血、增加血小板数和神经毒等作用。我所已于1984年合成了三七止血有效成分dencichine。
文摘The enantioseparation of dencichine and its D-isomer was achieved on a novel chiral stationary phase via coating N-（2-hydroxyl-3-octoxyl） propyl-S-benzyl-（L）-cysteine on YWG-C18 phase by ligand exchange chromatography.
文摘In order to quantitate dencichine in biological samples, a selective and sensitive method for the determination of dencichine in rat plasma based on high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) was developed and validated. (l)-2-amino-3-(carboxymethylthio)propionic acid was used as the internal standard (I.S.). After a protein precipitation extraction with acetonitrile, dencichine and the I.S. were chromatographed on an Xterra MS-C18 column. The mobile phase was consisted of 20mmol/L ammonium acetate solution-acetonitrile (35:65, V/V) at a flow rate of 0.2 mL/min. The detection was performed on a triple quadrupole mass via electrospray ionization (ESI) source in the positive mode. The intra- and inter-day precision (relative standard deviation, R.S.D.) values of dencichine were below 6.7%. The extraction recoveries were up 85%. The lower limit of quantification was 20 ng/ml, which was sensitive enough to detect the analyte. The HPLC-MS/MS method was successfully applied to the pharmacokinetic study after an intravenous administration of dencichine in Sprague-Dawley (SD) rat.
