[Objectives] To determine the optimal extraction technology of flavonoids in Dendranthema indicum,and analyze the antioxidant activity of total flavonoids in D. indicum. [Methods]D. indicum was used as raw material to...[Objectives] To determine the optimal extraction technology of flavonoids in Dendranthema indicum,and analyze the antioxidant activity of total flavonoids in D. indicum. [Methods]D. indicum was used as raw material to extract the total flavonoids by water extraction,alcohol extraction,freeze-thaw,microwave assisted extraction and ultrasonic extraction. [Results]Microwave assisted extraction had the highest extraction rate. Therefore,the microwave assisted extraction was selected as the main method,the ethanol aqueous solution was used as the solvent,and the single factor experiment was carried out with microwave power,ethanol volume fraction,solid-to-liquid ratio and microwave time as the variables. The optimal extraction process of flavonoids was microwave power 400 W,solid-to-liquid ratio 1 ∶ 30,extraction time60 s,ethanol volume fraction 70%,under this condition,the extraction yield was 10. 65 mg/g. The antioxidant activity of flavonoids in D. indicum was determined. The results showed that the highest scavenging rate of DPPH·free radicals was 47. 89%,and the highest scavenging rate of hydroxyl radicals was 98. 00%. [Conclusions]The flavonoids in D. indicum have excellent antioxidant activity. This study is expected to provide a basis for further development of D. indicum products.展开更多
The content of linatin,luteolin,quercetin and apigenin in Dendranthema indicum(L.) Des Moul.flower from eleven locations was analyzed by HPLC.The results show that linatin and luteolin contents in samples are higher w...The content of linatin,luteolin,quercetin and apigenin in Dendranthema indicum(L.) Des Moul.flower from eleven locations was analyzed by HPLC.The results show that linatin and luteolin contents in samples are higher with a range of 0.0-13.3 and 0.5-2.1 mg·g-1,respectively,and suitable for quantitative determination,while apigenin and quercetin contents are trace and suitable for qualitative determination.The content of flavonoids is obviously affected by locations.No linatin is detected in samples from Jiangxi and Nanyang of He’nan.The content of linatin and luteolin is higher in samples from Chuzhou of Anhui,Nanjing of Jiangsu and Inner Mongolia.D.indicum flower from Chuzhou of Anhui is superior for having all four flavonoids and higher content of linatin and luteolin.展开更多
Field cultivation experiments on white sesame(Sesamum indicum L.)seeds implanted with low-energy C ion showed that different dosages of C ion implantation pro-duce different biological effects.Sesame plants in 6 diffe...Field cultivation experiments on white sesame(Sesamum indicum L.)seeds implanted with low-energy C ion showed that different dosages of C ion implantation pro-duce different biological effects.Sesame plants in 6 different dosage groups with C ion density respectively at 1×10^(11),1×10^(12),1×10^(15),5×10^(15),1×10^(16),5×10^(16)ion/cm2 were superior to the control group in plant height,leaf number,stalk diameter and leaf size.Further,sesame plants in these groups flower and seed earlier than those in the control group,and single plant yield also increased.Of all the groups,the 5×10^(15)ion/cm2 dosage group yielded the best effect,whereas the 1×10^(17)/cm^(2)dosage group showed an evident inhibitory effect of ion implantation on the germination and growth of the sesame seeds.展开更多
基金Supported by Qing Lan Project of Universities in Jiangsu Province(2016)Brand Discipline Construction Project of Universities in Jiangsu Province(PPZY2015B179)+1 种基金Development Project for Biomass Green Fuel and Chemical Key Laboratory of Jiangsu Province(JSBGFC12010)Excellent Scientific and Technological Innovation Team of Universities in Jiangsu Province(2017-31)
文摘[Objectives] To determine the optimal extraction technology of flavonoids in Dendranthema indicum,and analyze the antioxidant activity of total flavonoids in D. indicum. [Methods]D. indicum was used as raw material to extract the total flavonoids by water extraction,alcohol extraction,freeze-thaw,microwave assisted extraction and ultrasonic extraction. [Results]Microwave assisted extraction had the highest extraction rate. Therefore,the microwave assisted extraction was selected as the main method,the ethanol aqueous solution was used as the solvent,and the single factor experiment was carried out with microwave power,ethanol volume fraction,solid-to-liquid ratio and microwave time as the variables. The optimal extraction process of flavonoids was microwave power 400 W,solid-to-liquid ratio 1 ∶ 30,extraction time60 s,ethanol volume fraction 70%,under this condition,the extraction yield was 10. 65 mg/g. The antioxidant activity of flavonoids in D. indicum was determined. The results showed that the highest scavenging rate of DPPH·free radicals was 47. 89%,and the highest scavenging rate of hydroxyl radicals was 98. 00%. [Conclusions]The flavonoids in D. indicum have excellent antioxidant activity. This study is expected to provide a basis for further development of D. indicum products.
文摘The content of linatin,luteolin,quercetin and apigenin in Dendranthema indicum(L.) Des Moul.flower from eleven locations was analyzed by HPLC.The results show that linatin and luteolin contents in samples are higher with a range of 0.0-13.3 and 0.5-2.1 mg·g-1,respectively,and suitable for quantitative determination,while apigenin and quercetin contents are trace and suitable for qualitative determination.The content of flavonoids is obviously affected by locations.No linatin is detected in samples from Jiangxi and Nanyang of He’nan.The content of linatin and luteolin is higher in samples from Chuzhou of Anhui,Nanjing of Jiangsu and Inner Mongolia.D.indicum flower from Chuzhou of Anhui is superior for having all four flavonoids and higher content of linatin and luteolin.
基金This work was supported by the Country Natural Sciences Fund Subsidizes Project(No.10435020)was the project of the Undergraduate Student Scientific Research Foundation Funds in Beijing Normal University.
文摘Field cultivation experiments on white sesame(Sesamum indicum L.)seeds implanted with low-energy C ion showed that different dosages of C ion implantation pro-duce different biological effects.Sesame plants in 6 different dosage groups with C ion density respectively at 1×10^(11),1×10^(12),1×10^(15),5×10^(15),1×10^(16),5×10^(16)ion/cm2 were superior to the control group in plant height,leaf number,stalk diameter and leaf size.Further,sesame plants in these groups flower and seed earlier than those in the control group,and single plant yield also increased.Of all the groups,the 5×10^(15)ion/cm2 dosage group yielded the best effect,whereas the 1×10^(17)/cm^(2)dosage group showed an evident inhibitory effect of ion implantation on the germination and growth of the sesame seeds.
