Objective:To isolate and characterize the bioactive secondary metabolites from Aspergillus ochraceus(A.ochraceus)MP2 fungi.Methods:The anti bacterial activity of marine sponge derived fungi A.ochraceus MP2 was thoroug...Objective:To isolate and characterize the bioactive secondary metabolites from Aspergillus ochraceus(A.ochraceus)MP2 fungi.Methods:The anti bacterial activity of marine sponge derived fungi A.ochraceus MP2 was thoroughly investigated against antagonistic human pathogens.The optimum inhibitory concentration of the fungi in the elite solvent was also determined.The promising extracts that showed good antimicrobial activity were subjected to further analytical separation to get individual distinct metabolites and the eluants were further identified by GC MS instrumental analysis.The molecular characterization of the elite fungal strains were done by isolating their genomic DNA and amplify the internal transcribed spacer(US)region of 5.8s rRNA using specific ITS primer.The novelty of the strain was proved by homology search tools and elite sequences was submitted to GENBANK.Results:Three bioactive compounds were characterized to reveal their identity,chemical formula and structure.The first elutant was identified asα-Campholene aldehyde with chemical fonnula C_(10)H_(16)O and molecular weight 152 Da.The second elutant was identified as Lucenin-2 and chemical fonnula C_(27)H_(30)O_(16)and molecular weight 610Da.The third elutant was identified as 6-Ethyloct-3-yl-2-ethylhexyl ester with Chemical fonnula C_(26)H_(42)O_4 with molecular weight 418 Da.Conclusions:The isolated compounds showed significant antimicrobial activity against potential human pathogens.Microbial secondary metabolites represent a large source of compounds endowed with ingenious structures and potent biological activities.展开更多
This review explores the natural products of seagrass that are to be exploited for their bioactive potential.Beside from portraying the presence of a wide array of secondary compounds such as phenols,flavonoids,sterol...This review explores the natural products of seagrass that are to be exploited for their bioactive potential.Beside from portraying the presence of a wide array of secondary compounds such as phenols,flavonoids,sterols and lipids from different seagrass species,the focus is on novel natural products projecting towards their biological applications.Though there are a significant number of reports on the abundance of secondary metabolites from seagrass and their bioactive derivatives,only a small number of reports explore their functional and defensive characteristics.Efforts have been made to collate the available information on seagrass natural products and clarify their function and metabolic pathway’s.It is emphasized that metabolic profiling of seagrass should be extensively progressed to obtain a deeper knowledge about the specific roles of each natural product.The investigation of seagrass natural products for their bioactive potential would most likely result in the detection of surprising and unexpected novel chemical structures and clinical leads that may be useful to mankind.展开更多
Three new amide derivatives(alteralkaloids A-C,1-3)and three known alkaloids(4-6)were afforded after phytochemical investigation of fungus Alternaria brassicicola.The structures of these compounds were confirmed by NM...Three new amide derivatives(alteralkaloids A-C,1-3)and three known alkaloids(4-6)were afforded after phytochemical investigation of fungus Alternaria brassicicola.The structures of these compounds were confirmed by NMR spectroscopic and HRESIMS data.Furthermore,the absolute configuration of 1 was determined using the single-crystal X-ray diffraction analysis.Compounds 1-3 belong to a class of amide derivatives that have not been found in nature before,sharing the same characteristic signals of the butyl moiety and amide group.These isolated compounds mentioned above were tested for the cytotoxic activity.展开更多
Objective: To study secondary metabolites from endophytic fungus Colletotruchum sp. HK-08 originated from the leaves of Nerium indicum.Methods: The compounds were isolated by various column chromatographic techniques,...Objective: To study secondary metabolites from endophytic fungus Colletotruchum sp. HK-08 originated from the leaves of Nerium indicum.Methods: The compounds were isolated by various column chromatographic techniques, and their structures were elucidated by spectroscopic techniques [high resolution electrospray ionization mass spectroscopy(HRESIMS), one-dimensional(1D) and two-dimensional(2D) nuclear magnetic resonance spectroscopy(NMR)], as well as comparison with literature data. The Ellman method was used to determine the acetylcholinesterase(ACh E) inhibitory activity.Results: Four indole derivatives were identified from Colletotruchum sp. HK-08, including 6’-hydroxymonaspiloindole(1), 2-(2-oxoindolin-3-yl)ethyl 2-(4-hydroxyphenyl) acetate(2), 2-(2-oxoindolin-3-yl)ethyl 2-(2-hydroxyphenyl)acetate(3), and monaspiloindole(4). Compound 4 presented weak ACh E inhibitory activity with IC50value of(69.30 ± 6.27) μmol/L [tacrine as the positive control, with IC50value of(0.61 ± 0.07) μmol/L].Conclusion: Compounds 1–3 were new compounds, and compound 4 had weak ACh E inhibitory activity.展开更多
A new polyoxygenated dihydropyrano[2,3-c]pyrrole-4,5-dione derivative, pyranonigrin F （1）, together with a related known compound, pyranonigrin A （2）, were isolated and identified from Penicillium brocae MA-231, a...A new polyoxygenated dihydropyrano[2,3-c]pyrrole-4,5-dione derivative, pyranonigrin F （1）, together with a related known compound, pyranonigrin A （2）, were isolated and identified from Penicillium brocae MA-231, an endophytic fungus obtained from the fresh tissue of the marine mangrove plant Avicennia marina. The structures of these metabolites were determined based on comprehensive spectral interpretation and the absolute configuration of compound 1 was established by X-ray crystallographic analysis. Compounds 1 and 2 showed potent activity against a broad spectrum of human-, aqua-, and plant-pathogens.展开更多
文摘Objective:To isolate and characterize the bioactive secondary metabolites from Aspergillus ochraceus(A.ochraceus)MP2 fungi.Methods:The anti bacterial activity of marine sponge derived fungi A.ochraceus MP2 was thoroughly investigated against antagonistic human pathogens.The optimum inhibitory concentration of the fungi in the elite solvent was also determined.The promising extracts that showed good antimicrobial activity were subjected to further analytical separation to get individual distinct metabolites and the eluants were further identified by GC MS instrumental analysis.The molecular characterization of the elite fungal strains were done by isolating their genomic DNA and amplify the internal transcribed spacer(US)region of 5.8s rRNA using specific ITS primer.The novelty of the strain was proved by homology search tools and elite sequences was submitted to GENBANK.Results:Three bioactive compounds were characterized to reveal their identity,chemical formula and structure.The first elutant was identified asα-Campholene aldehyde with chemical fonnula C_(10)H_(16)O and molecular weight 152 Da.The second elutant was identified as Lucenin-2 and chemical fonnula C_(27)H_(30)O_(16)and molecular weight 610Da.The third elutant was identified as 6-Ethyloct-3-yl-2-ethylhexyl ester with Chemical fonnula C_(26)H_(42)O_4 with molecular weight 418 Da.Conclusions:The isolated compounds showed significant antimicrobial activity against potential human pathogens.Microbial secondary metabolites represent a large source of compounds endowed with ingenious structures and potent biological activities.
文摘This review explores the natural products of seagrass that are to be exploited for their bioactive potential.Beside from portraying the presence of a wide array of secondary compounds such as phenols,flavonoids,sterols and lipids from different seagrass species,the focus is on novel natural products projecting towards their biological applications.Though there are a significant number of reports on the abundance of secondary metabolites from seagrass and their bioactive derivatives,only a small number of reports explore their functional and defensive characteristics.Efforts have been made to collate the available information on seagrass natural products and clarify their function and metabolic pathway’s.It is emphasized that metabolic profiling of seagrass should be extensively progressed to obtain a deeper knowledge about the specific roles of each natural product.The investigation of seagrass natural products for their bioactive potential would most likely result in the detection of surprising and unexpected novel chemical structures and clinical leads that may be useful to mankind.
基金the National Program for Support of Top-notch Young Professionals(No.0106514050)the National NSFC(Nos.82273811 and 82104043)+3 种基金the National Key R&D Program of China(No.2021YFA0910500)the National NSF for Distinguished Young Scholars(No.81725021)the Innovative Research Groups of the National NSFC(No.81721005)the Academic Frontier Youth Team of HUST(No.2017QYTD19).
文摘Three new amide derivatives(alteralkaloids A-C,1-3)and three known alkaloids(4-6)were afforded after phytochemical investigation of fungus Alternaria brassicicola.The structures of these compounds were confirmed by NMR spectroscopic and HRESIMS data.Furthermore,the absolute configuration of 1 was determined using the single-crystal X-ray diffraction analysis.Compounds 1-3 belong to a class of amide derivatives that have not been found in nature before,sharing the same characteristic signals of the butyl moiety and amide group.These isolated compounds mentioned above were tested for the cytotoxic activity.
基金supported by the Hainan Natural Science Foundation(No.220RC702).
文摘Objective: To study secondary metabolites from endophytic fungus Colletotruchum sp. HK-08 originated from the leaves of Nerium indicum.Methods: The compounds were isolated by various column chromatographic techniques, and their structures were elucidated by spectroscopic techniques [high resolution electrospray ionization mass spectroscopy(HRESIMS), one-dimensional(1D) and two-dimensional(2D) nuclear magnetic resonance spectroscopy(NMR)], as well as comparison with literature data. The Ellman method was used to determine the acetylcholinesterase(ACh E) inhibitory activity.Results: Four indole derivatives were identified from Colletotruchum sp. HK-08, including 6’-hydroxymonaspiloindole(1), 2-(2-oxoindolin-3-yl)ethyl 2-(4-hydroxyphenyl) acetate(2), 2-(2-oxoindolin-3-yl)ethyl 2-(2-hydroxyphenyl)acetate(3), and monaspiloindole(4). Compound 4 presented weak ACh E inhibitory activity with IC50value of(69.30 ± 6.27) μmol/L [tacrine as the positive control, with IC50value of(0.61 ± 0.07) μmol/L].Conclusion: Compounds 1–3 were new compounds, and compound 4 had weak ACh E inhibitory activity.
基金Financial support from the National Natural Science Foundation of China(No.31270403)from the Ministry of Science and Technology of China(No.2010CB833802) is gratefully acknowledged
文摘A new polyoxygenated dihydropyrano[2,3-c]pyrrole-4,5-dione derivative, pyranonigrin F （1）, together with a related known compound, pyranonigrin A （2）, were isolated and identified from Penicillium brocae MA-231, an endophytic fungus obtained from the fresh tissue of the marine mangrove plant Avicennia marina. The structures of these metabolites were determined based on comprehensive spectral interpretation and the absolute configuration of compound 1 was established by X-ray crystallographic analysis. Compounds 1 and 2 showed potent activity against a broad spectrum of human-, aqua-, and plant-pathogens.
