B链氨端去－肽（B1）羧端去五肽（B26—30）猪胰岛素的初步晶体学研究

应用悬滴气相扩散法在含６％氯化纳和１２．５％丙酮的柠檬酸缓冲体系中，获得可供Ｘ射线结构分析用的Ｂ链氨端去一肽（Ｂ１）羧端去五肽（Ｂ２６—３０）猪胰岛素（ＤｅｓＢ１－ＤＰＩ）单晶体。晶体属四方晶系，空间群为Ｐ４１２２或Ｐ４３２２，晶胞参数为：ａ＝ｂ＝３６．０Ａ，ｃ＝１２０．０Ａ，ａ＝β＝γ＝９０°。单位晶胞中每个结晶学不对称单位含有２个ＤｅｓＢ１—ＤＰＩ分子。

Preliminary Crystallographic Studies of DesB1- 2-Despentapeptide (B260-30) Insulin

In order to explore the specific structure of the insulin analogues and the motion way of B-chain C-terminal peptide, Liang considered the amphipathic surface of insulin molecule to be the binding interaction surface with its receptor and the conformational changes at B-chain C-terminus of insulin during the binding