Sensory inputs stimulated by Zusanli（ST36） acupuncture in the abdomen are known to converge in the upper cervical cord. However, it is unclear whether these inputs are subsequently conveyed to the hypothalamic parav...Sensory inputs stimulated by Zusanli（ST36） acupuncture in the abdomen are known to converge in the upper cervical cord. However, it is unclear whether these inputs are subsequently conveyed to the hypothalamic paraventricular nucleus and what kind of afferent fibers are involved. We focused on the upper cervical cord, where afferent inputs converge, and detected c-fos expression in oxytocinergic neurons. We found that Zusanli acupuncture therapy effectively elevated intragastric pressure, but inhibited expression of c-fos in oxytocinergic neurons of the paraventricular nucleus in upper cervical cord injured rats. These Zusanli acupuncture effects remained even after complete dorsal cord transection. However, after complete transection of the spinal cord or dorsolateral funiculus, the effects were significantly attenuated and even disappeared. These findings suggest that the paraventricular nucleus is responsible for pooling and integrating signals from the Zusanli acupuncture and sensory information from the intragastric pressure variation, thereby contributing to the regulation of intragastric pressure. The upper cervical cord serves as the key link between ascending and descending pathways, which conveys afferent inputs to the paraventricular nucleus through the dorsolateral funiculus.展开更多
Aim: Repeated microinjections of non-opioid an-algesics into the midbrain periaqueductal gray matter and rostral ventro-medial medulla induce antinociception with development of tolerance. Antinociception following sy...Aim: Repeated microinjections of non-opioid an-algesics into the midbrain periaqueductal gray matter and rostral ventro-medial medulla induce antinociception with development of tolerance. Antinociception following systemic administra-tion of non-steroidal anti-inflammatory drugs (N SAIDs) also exhibit tolerance. Presently our aim was to investigate the development of tolerance to the antinociceptive effects of NSAIDs analgine, ketorolac, and xefocam microinjected into cen-tral nucleus of amygdala (Ce) in rats. Methods: Under anesthesia with thiopental a stainless steel guide cannula was stereotaxically implanted uni- laterally or bilaterally into the Ce using stereo-taxic atlas coordinates, and anchored to the cra- nium by dental cement. Five days after surgery, 3 μl of these NSAIDs were injected via the injec-tion cannula while the rat was gently restrained. Twenty min post microinjection, i.e. 10-min be-fore the peak of the drugs’ effect is normally rea- ched, animals were tested with tail flick (TF) and hot plate (HP) tests. On the 5th experimental day all animals received a Ce microinjection of mor-phine. Results: Daily microinjection of NSAIDs into the Ce uni- or bilaterally, produced antino-ciception with development of complete toler-ance over a 5-day period. Following the treat-ment period, morphine microinjection into the Ce failed to elicit antinociception, indicating cro- ss-tolerance to the antinociceptive effect of N SAIDs. In other words, the “non-opioid tolerant” rats showed cross-tolerance to morphine. Con-clusions: Our data confirmed the suggestion that NSAIDs interact with endogenous opioid systems, which likely play a key role in the development of tolerance to the antinociceptive effects of NSA IDs.展开更多
目的:观察对侧网状巨细胞核(NGC)对脊髓伤害性信息传递的调制作用。方法:以浅麻醉和麻痹大鼠为对象,利用计算机平均技术,记录强电流刺激腓肠神经诱发的脊髓背表面电位(CDP),观察对侧网状巨细胞核微量注射 L-谷氨酸钠,使神经细胞体兴奋后...目的:观察对侧网状巨细胞核(NGC)对脊髓伤害性信息传递的调制作用。方法:以浅麻醉和麻痹大鼠为对象,利用计算机平均技术,记录强电流刺激腓肠神经诱发的脊髓背表面电位(CDP),观察对侧网状巨细胞核微量注射 L-谷氨酸钠,使神经细胞体兴奋后对 CDP 的影响。对照组向对侧 NGC 内注射等量的人工脑脊液。CDP 变化用对照的百分数表示,统计学分析采用 t 检验,以 P<0.05为有显著性意义。结果:对侧 NGC 微量注射 L-谷氨酸钠后,CDP 中N_1波无显著性改变(P>0.05),P_1波及 N_3波分别减少到对照的70.68%±2.66%(P<0.01)和78.94%±7.91%(P<0.05),P_2波明显减小到对照的4.33%±3.1%(P<0.01),N_4波消失。结论:对侧 NGC 对细纤维诱发的 CDP 成分有抑制作用。展开更多
It has been shown in our previous work that the impulses from acupuncture points might inhibit the impulses from pain points through presynaptic inhibition in the spinal tract nucleus of trigeminal nerve. The stimulat...It has been shown in our previous work that the impulses from acupuncture points might inhibit the impulses from pain points through presynaptic inhibition in the spinal tract nucleus of trigeminal nerve. The stimulation of nucleus raphe magnus might significantly increase the effect of acupuncture analgesia in spinal tract nucleus of trigeminal nerve. It has also been shown that there are descending展开更多
以标题结合主题词检索方式从CNKI,Pubmed,Web of science等数据库筛查出近20年中药干预神经病理性疼痛(neuropathic pain,NP)作用机制的文献70多篇,进行归纳分析。发现多种中药对NP有良好的治疗作用,其机制主要表现为对NP下行抑制/易化...以标题结合主题词检索方式从CNKI,Pubmed,Web of science等数据库筛查出近20年中药干预神经病理性疼痛(neuropathic pain,NP)作用机制的文献70多篇,进行归纳分析。发现多种中药对NP有良好的治疗作用,其机制主要表现为对NP下行抑制/易化系统、离子通道、膜受体、神经炎症及胶质细胞活化、相关信号通路及调控原件、抗凋亡和氧化应激、抑制酶活性等方面的有效调节,且具有多靶点干预的特点,不仅能抑制神经冲动,亦能有效改善NP的神经源性炎症。目前,中医药对NP神经炎症的干预逐渐引起学者们的关注,但相关机制研究还不深入。展开更多
基金supported by the National Natural Science Foundation of China,No.81273677a grant from the Postgraduate Innovation Topics of Hunan University of Chinese Medicine in China in 2014,No.2014CX01
文摘Sensory inputs stimulated by Zusanli（ST36） acupuncture in the abdomen are known to converge in the upper cervical cord. However, it is unclear whether these inputs are subsequently conveyed to the hypothalamic paraventricular nucleus and what kind of afferent fibers are involved. We focused on the upper cervical cord, where afferent inputs converge, and detected c-fos expression in oxytocinergic neurons. We found that Zusanli acupuncture therapy effectively elevated intragastric pressure, but inhibited expression of c-fos in oxytocinergic neurons of the paraventricular nucleus in upper cervical cord injured rats. These Zusanli acupuncture effects remained even after complete dorsal cord transection. However, after complete transection of the spinal cord or dorsolateral funiculus, the effects were significantly attenuated and even disappeared. These findings suggest that the paraventricular nucleus is responsible for pooling and integrating signals from the Zusanli acupuncture and sensory information from the intragastric pressure variation, thereby contributing to the regulation of intragastric pressure. The upper cervical cord serves as the key link between ascending and descending pathways, which conveys afferent inputs to the paraventricular nucleus through the dorsolateral funiculus.
文摘Aim: Repeated microinjections of non-opioid an-algesics into the midbrain periaqueductal gray matter and rostral ventro-medial medulla induce antinociception with development of tolerance. Antinociception following systemic administra-tion of non-steroidal anti-inflammatory drugs (N SAIDs) also exhibit tolerance. Presently our aim was to investigate the development of tolerance to the antinociceptive effects of NSAIDs analgine, ketorolac, and xefocam microinjected into cen-tral nucleus of amygdala (Ce) in rats. Methods: Under anesthesia with thiopental a stainless steel guide cannula was stereotaxically implanted uni- laterally or bilaterally into the Ce using stereo-taxic atlas coordinates, and anchored to the cra- nium by dental cement. Five days after surgery, 3 μl of these NSAIDs were injected via the injec-tion cannula while the rat was gently restrained. Twenty min post microinjection, i.e. 10-min be-fore the peak of the drugs’ effect is normally rea- ched, animals were tested with tail flick (TF) and hot plate (HP) tests. On the 5th experimental day all animals received a Ce microinjection of mor-phine. Results: Daily microinjection of NSAIDs into the Ce uni- or bilaterally, produced antino-ciception with development of complete toler-ance over a 5-day period. Following the treat-ment period, morphine microinjection into the Ce failed to elicit antinociception, indicating cro- ss-tolerance to the antinociceptive effect of N SAIDs. In other words, the “non-opioid tolerant” rats showed cross-tolerance to morphine. Con-clusions: Our data confirmed the suggestion that NSAIDs interact with endogenous opioid systems, which likely play a key role in the development of tolerance to the antinociceptive effects of NSA IDs.
文摘目的:观察对侧网状巨细胞核(NGC)对脊髓伤害性信息传递的调制作用。方法:以浅麻醉和麻痹大鼠为对象,利用计算机平均技术,记录强电流刺激腓肠神经诱发的脊髓背表面电位(CDP),观察对侧网状巨细胞核微量注射 L-谷氨酸钠,使神经细胞体兴奋后对 CDP 的影响。对照组向对侧 NGC 内注射等量的人工脑脊液。CDP 变化用对照的百分数表示,统计学分析采用 t 检验,以 P<0.05为有显著性意义。结果:对侧 NGC 微量注射 L-谷氨酸钠后,CDP 中N_1波无显著性改变(P>0.05),P_1波及 N_3波分别减少到对照的70.68%±2.66%(P<0.01)和78.94%±7.91%(P<0.05),P_2波明显减小到对照的4.33%±3.1%(P<0.01),N_4波消失。结论:对侧 NGC 对细纤维诱发的 CDP 成分有抑制作用。
文摘It has been shown in our previous work that the impulses from acupuncture points might inhibit the impulses from pain points through presynaptic inhibition in the spinal tract nucleus of trigeminal nerve. The stimulation of nucleus raphe magnus might significantly increase the effect of acupuncture analgesia in spinal tract nucleus of trigeminal nerve. It has also been shown that there are descending
文摘以标题结合主题词检索方式从CNKI,Pubmed,Web of science等数据库筛查出近20年中药干预神经病理性疼痛(neuropathic pain,NP)作用机制的文献70多篇,进行归纳分析。发现多种中药对NP有良好的治疗作用,其机制主要表现为对NP下行抑制/易化系统、离子通道、膜受体、神经炎症及胶质细胞活化、相关信号通路及调控原件、抗凋亡和氧化应激、抑制酶活性等方面的有效调节,且具有多靶点干预的特点,不仅能抑制神经冲动,亦能有效改善NP的神经源性炎症。目前,中医药对NP神经炎症的干预逐渐引起学者们的关注,但相关机制研究还不深入。
