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AIRCRAFT DESIGN EXPERIENCE
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作者 S.Kikuhara 《Chinese Journal of Aeronautics》 SCIE EI CAS CSCD 1991年第2期87-98,共12页
Some of the author's experiences of aircraft design are described in the present report, and it is hoped that they would be of some interest to the aircraft designers.
关键词 AIRCRAFT design experience
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DADOS:A Cloud-based Data-driven Design Optimization System 被引量:2
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作者 Xueguan Song Shuo Wang +2 位作者 Yonggang Zhao Yin Liu Kunpeng Li 《Chinese Journal of Mechanical Engineering》 SCIE EI CAS CSCD 2023年第2期50-66,共17页
This paper presents a cloud-based data-driven design optimization system,named DADOS,to help engineers and researchers improve a design or product easily and efficiently.DADOS has nearly 30 key algorithms,including th... This paper presents a cloud-based data-driven design optimization system,named DADOS,to help engineers and researchers improve a design or product easily and efficiently.DADOS has nearly 30 key algorithms,including the design of experiments,surrogate models,model validation and selection,prediction,optimization,and sensitivity analysis.Moreover,it also includes an exclusive ensemble surrogate modeling technique,the extended hybrid adaptive function,which can make use of the advantages of each surrogate and eliminate the effort of selecting the appropriate individual surrogate.To improve ease of use,DADOS provides a user-friendly graphical user interface and employed flow-based programming so that users can conduct design optimization just by dragging,dropping,and connecting algorithm blocks into a workflow instead of writing massive code.In addition,DADOS allows users to visualize the results to gain more insights into the design problems,allows multi-person collaborating on a project at the same time,and supports multi-disciplinary optimization.This paper also details the architecture and the user interface of DADOS.Two examples were employed to demonstrate how to use DADOS to conduct data-driven design optimization.Since DADOS is a cloud-based system,anyone can access DADOS at www.dados.com.cn using their web browser without the need for installation or powerful hardware. 展开更多
关键词 DATA-DRIVEN OPTIMIZATION Cloud-based software design of experiments Surrogate model
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Design of Ecotourism Experience in Shennongjia
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作者 WU Qianni 《Journal of Landscape Research》 2021年第3期129-131,134,共4页
Ecotourism experience means that tourists obtain ecological enjoyment,ecological perception and ecological aesthetic taste through the senses and thinking activities in ecotourism activities.Shennongjia has excellent ... Ecotourism experience means that tourists obtain ecological enjoyment,ecological perception and ecological aesthetic taste through the senses and thinking activities in ecotourism activities.Shennongjia has excellent ecological environment,but lacks deep experience ecotourism products.Based on the principles of ecotourism experience design,the ecotourism experience theme,ecotourism experience product and ecotourism experience atmosphere of Shennongjia are designed. 展开更多
关键词 Ecotourism experience Shennongjia experience design
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A Brief Talk on Environmental Experience Graphic Design in the Construction of Characteristic Tourism From Signage Design
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作者 ZHONG Cheng 《Psychology Research》 2022年第6期340-346,共7页
With the continuous development of the national economy and the improvement of the design industry,the wayfinding system has gradually been accepted and valued by the public,and the research on the wayfinding system o... With the continuous development of the national economy and the improvement of the design industry,the wayfinding system has gradually been accepted and valued by the public,and the research on the wayfinding system of the public environment has become more and more in-depth.In recent years,the research on the wayfinding system has begun to develop into the integration of human culture and cultural implantation in the environment,forming a new visual system design concept,Environment Graphic Design.With the deepening of research,environmental graphic design has once again developed and deepened,studying the detailed experience of people in the environment and presenting it in the form of graphic design.That is,Experience Graphic Design.This article will discuss the application of environmental graphic experience design in the construction of characteristic tourism from the perspective of signage design. 展开更多
关键词 INFRASTRUCTURE characteristic tourism HOMOGENIZATION environmental experience graphic design
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Brand communication by brand experience design of APP for hotel
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作者 Zhe Huang Linlin He 《International English Education Research》 2015年第8期29-32,共4页
In the era of experience economy, customers do not always focus on the functional attributes of goods or services, but pay more attention to experiences. Therefore, firms have to manage to offer their customers a uniq... In the era of experience economy, customers do not always focus on the functional attributes of goods or services, but pay more attention to experiences. Therefore, firms have to manage to offer their customers a unique, memorable and valuable experience actively in the process brand establishment. With the development of mobile Internet and the use of application (APP), the brand communication among customers and providers has been strengthened. The research purpose is to help hotel marketers figure out how to implement brand communication with brand experience of APP for hotel. It is founded that sensory brand experience design and social brand experience all have positive influence on customer brand loyalty. 展开更多
关键词 brand communication brand experience design APP aesthetic value SELF-WORTH brand loyalty
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Process Characterization of the Transesterification of Rapeseed Oil to Biodiesel Using Design of Experiments and Infrared Spectroscopy
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作者 Tobias Drieschner Andreas Kandelbauer +1 位作者 Bernd Hitzmann Karsten Rebner 《Journal of Renewable Materials》 SCIE EI 2023年第4期1643-1660,共18页
For optimization of production processes and product quality,often knowledge of the factors influencing the process outcome is compulsory.Thus,process analytical technology(PAT)that allows deeper insight into the proc... For optimization of production processes and product quality,often knowledge of the factors influencing the process outcome is compulsory.Thus,process analytical technology(PAT)that allows deeper insight into the process and results in a mathematical description of the process behavior as a simple function based on the most important process factors can help to achieve higher production efficiency and quality.The present study aims at characterizing a well-known industrial process,the transesterification reaction of rapeseed oil with methanol to produce fatty acid methyl esters(FAME)for usage as biodiesel in a continuous micro reactor set-up.To this end,a design of experiment approach is applied,where the effects of two process factors,the molar ratio and the total flow rate of the reactants,are investigated.The optimized process target response is the FAME mass fraction in the purified nonpolar phase of the product as a measure of reaction yield.The quantification is performed using attenuated total reflection infrared spectroscopy in combination with partial least squares regression.The data retrieved during the conduction of the DoE experimental plan were used for statistical analysis.A non-linear model indicating a synergistic interaction between the studied factors describes the reactor behavior with a high coefficient of determination(R^(2))of 0.9608.Thus,we applied a PAT approach to generate further insight into this established industrial process. 展开更多
关键词 Process analytical technology TRANSESTERIFICATION design of experiment attenuated total reflection infrared spectroscopy partial least square regression
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Factorial Experimental Design to Study the Effects of Layers and Fiber Content on Concrete Flexural Behavior
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作者 Dumbiri H. Odia 《Open Journal of Civil Engineering》 CAS 2023年第1期83-102,共20页
Experimentation has come a long in helping researchers achieve breakthroughs in their different scientific areas and engineering happens to be one of those areas with the most impact from experimental advancement. The... Experimentation has come a long in helping researchers achieve breakthroughs in their different scientific areas and engineering happens to be one of those areas with the most impact from experimental advancement. The need for valid experimental results free from biases and confounding conclusions has prompted the development of new experimental techniques that takes consideration of all applicable factor and combinations in providing answers on a research topic, and the Factorial Experimental design credited to Sir Ronald Fisher is one technique yielding highly valid results. This paper uses the factorial design of experiments to research the flexural impact of polyvinyl acetate fiber and layered concrete in construction. The experiment considered two levels of fiber contents and two levels of layers, and prepared samples with all combinations of the variable factors. The samples were tested after 7 days from casting for flexural strength and an advance statistical analysis was performed on the flexural responses of the samples using R-program. The results from the analyses revealed the significance of the variables to the flexural strength of the samples, as well as their interactions. The experiment concluded that based on the number of layers and fiber content used for the experiment, casting concrete in layers does have a significant negative effect on the flexural strength of concrete, and the failure pattern of concrete members under flexural load in evidently influenced by the material composition of the concrete, and that it can be evidently influenced by casting the concrete in layers. 展开更多
关键词 Experimental design Concrete Flexural Strength Factorial design of Experiments Concrete Fibers Concrete Layers
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Preliminary Study on the Low Energy Ion Beam Mediated Parameters with Fractional Factorial Design Method 被引量:2
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作者 陈雪能 黄群策 赵帅鹏 《Agricultural Science & Technology》 CAS 2010年第8期137-139,共3页
[Objective] The aim was to explore the biological effect of low energy ion beam mediated parameters with fractional factorial design method. [Method] The twin-embryos seed of autotetraploid rice DER10-04-01 was taken ... [Objective] The aim was to explore the biological effect of low energy ion beam mediated parameters with fractional factorial design method. [Method] The twin-embryos seed of autotetraploid rice DER10-04-01 was taken as the receptor material,and the Elymus dahuricus Turcz. was used as materials to provide DNA to carry out the ion beam mediated experiment. And the fractional factorial design method was used to study the parameters of low enery N+ ion beam mediated foreign genes into rice. [Result] The implantation energy,dose,DNA concentrations and immersion time of DNA showed significant biological effects on the normal growth and development of DER10-04-01,in which the biological effects of implantation dose and DNA concentrations were relatively obvious. [Conclusion] The implantation energy,dose,DNA concentrations and immersion time of DNA were major factors showing important effects on the experimental result in ion beam mediated foreign genetic materials. 展开更多
关键词 Ion beam Factorial experiment design Rice with twin-embryos seedlings Elymus dahuricus Turcz.
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Device Robust-Design by Using Response Surface Methodology
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作者 谢晓锋 鲁勇 +1 位作者 张文俊 杨之廉 《Journal of Semiconductors》 EI CAS CSCD 北大核心 2002年第8期817-824,共8页
Device robust-design is inherently a multiple-objective optimization problem.Using design of experiments (DoE) combined with response surface methodology (RSM) can satisfy the great incentive to reduce the number of t... Device robust-design is inherently a multiple-objective optimization problem.Using design of experiments (DoE) combined with response surface methodology (RSM) can satisfy the great incentive to reduce the number of technology CAD(TCAD) simulations that need to be performed.However,the errors of RSM models might be large enough to diminish the validity of the results for some nonlinear problems.To find the feasible design space,a new method with objectives-oriented design in generations that takes the errors of RSM model into account is presented.After the augment design of experiments in promising space according to the results of RSM model in current generation,the feasible space will be emerging as the model errors deceasing.The results on FIBMOS examples show that the methodology is efficient. 展开更多
关键词 response surface methodology design of experiments device robust-design
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Optimizing Bucket Elevator Performance through a Blend of Discrete Element Method, Response Surface Methodology, and Firefly Algorithm Approaches
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作者 Pirapat Arunyanart Nithitorn Kongkaew Supattarachai Sudsawat 《Computers, Materials & Continua》 SCIE EI 2024年第8期3379-3403,共25页
This research introduces a novel approach to enhancing bucket elevator design and operation through the integration of discrete element method(DEM)simulation,design of experiments(DOE),and metaheuristic optimization a... This research introduces a novel approach to enhancing bucket elevator design and operation through the integration of discrete element method(DEM)simulation,design of experiments(DOE),and metaheuristic optimization algorithms.Specifically,the study employs the firefly algorithm(FA),a metaheuristic optimization technique,to optimize bucket elevator parameters for maximizing transport mass and mass flow rate discharge of granular materials under specified working conditions.The experimental methodology involves several key steps:screening experiments to identify significant factors affecting bucket elevator operation,central composite design(CCD)experiments to further explore these factors,and response surface methodology(RSM)to create predictive models for transport mass and mass flow rate discharge.The FA algorithm is then applied to optimize these models,and the results are validated through simulation and empirical experiments.The study validates the optimized parameters through simulation and empirical experiments,comparing results with DEM simulation.The outcomes demonstrate the effectiveness of the FA algorithm in identifying optimal bucket parameters,showcasing less than 10%and 15%deviation for transport mass and mass flow rate discharge,respectively,between predicted and actual values.Overall,this research provides insights into the critical factors influencing bucket elevator operation and offers a systematic methodology for optimizing bucket parameters,contributing to more efficient material handling in various industrial applications. 展开更多
关键词 Discrete element method(DEM) design of experiments(DOE) firefly algorithm(FA) response surface methodology(RSM)
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Cavitation Optimization for a Centrifugal Pump Impeller by Using Orthogonal Design of Experiment 被引量:6
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作者 PEI Ji YIN Tingyun +2 位作者 YUAN Shouqi WANG Wenjie WANG Jiabin 《Chinese Journal of Mechanical Engineering》 SCIE EI CAS CSCD 2017年第1期103-109,共7页
Cavitation is one of the most important performance of centrifugal pumps. However, the current optimization works of centrifugal pump are mostly focusing on hydraulic efficiency only, which may result in poor cavitati... Cavitation is one of the most important performance of centrifugal pumps. However, the current optimization works of centrifugal pump are mostly focusing on hydraulic efficiency only, which may result in poor cavitation performance. Therefore, it is necessary to find an appropriate solution to improve cavitation performance with acceptable efficiency. In this paper, to improve the cavitation performance of a centrifugal pump with a vaned diffuser, the influence of impeller geometric parameters on the cavitation of the pump is investigated using the orthogonal design of experiment (DOE) based on computational fluid dynamics. The impeller inlet diameter D1, inlet incidence angle Aft, and blade wrap angle ~0 are selected as the main impeller geometric parameters and the orthogonal experiment of L9(3"3) is performed. Three-dimensional steady simulations for cavitation are conducted by using constant gas mass fraction model with second-order upwind, and the predicated cavitation performance is validated by laboratory experiment. The optimization results are obtained by the range analysis method to improve cavitation performance without obvious decreasing the efficiency of the centrifugal pump. The internal flow of the pump is analyzed in order to identify the flow behavior that can affect cavitation performance. The results show that D1 has the greatest influence on the pump cavitation and the final optimized impeller provides better flow distribution at blade leading edge. The final optimized impeller accomplishes better cavitation and hydraulic performance and the NPSHR decreases by 0.63m compared with the original one. The presented work supplies a feasible route in engineering practice to optimize a centrifugal pump impeller for better cavitation performance. 展开更多
关键词 cavitation optimization computational fluid dynamics orthogonal design of experiment range analysis
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Study on solder joint reliability of ceramic ball grid array component based on design of experiment method 被引量:6
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作者 吴兆华 黄春跃 周德俭 《China Welding》 EI CAS 2006年第3期68-73,共6页
Four process parameters, pad diameter, stencil thickness, ball diameter and stand-off were chosen as four control factors. By using an L25 (5^6 ) orthogonal array the ceramic ball grid array ( CBGA ) solder joints... Four process parameters, pad diameter, stencil thickness, ball diameter and stand-off were chosen as four control factors. By using an L25 (5^6 ) orthogonal array the ceramic ball grid array ( CBGA ) solder joints which have 25 different combinations of process parameters were designed. The numerical models of all the 25 CBGA solder joints were developed using the Sugrace Evolver. Utilizing the sugrace coordinate exported from the 25 CBGA solder joints numerical models, the finite element analysis models were set up and the nonlinear finite element analysis of the CBGA solder joints under thermal cycles were pegrormed by ANSYS. The thermal fatigue life of CBGA solder joint was calculated using Coffin-Manson equation. Based on the calculated thermal fatigue life results, the range analysis and the variance analysis were pegrormed. The results show that the fatigue life of CBGA solder joint is affected by the pad diameter, the stencil thickness, the ball diameter and the stand-off in a descending order, the best combination of process parameters results in the longest fatigue life is 0.07 mm stand-off, 0.125 mm stencil thickness of, 0.85 mm ball diameter and 0. 89 mm pad diameter. With 95% confidence the pad diameter has a significant effect on the reliability of CBGA solder joints whereas the stand-off, the stencil thickness and the ball diameter have little effect on the reliability of CBGA solder joints. 展开更多
关键词 design of experiment ceramic ball grid array solder joint process parameters finite element analysis
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Modeling Approach and Analysis of the Structural Parameters of an Inductively Coupled Plasma Etcher Based on a Regression Orthogonal Design 被引量:3
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作者 程嘉 朱煜 季林红 《Plasma Science and Technology》 SCIE EI CAS CSCD 2012年第12期1059-1068,共10页
The geometry of an inductively coupled plasma (ICP) etcher is usually considered to be an important factor for determining both plasma and process uniformity over a large wafer. During the past few decades, these pa... The geometry of an inductively coupled plasma (ICP) etcher is usually considered to be an important factor for determining both plasma and process uniformity over a large wafer. During the past few decades, these parameters were determined by the "trial and error" method, resulting in wastes of time and funds. In this paper, a new approach of regression orthogonal design with plasma simulation experiments is proposed to investigate the sensitivity of the structural parameters on the uniformity of plasma characteristics. The tool for simulating plasma is CFD-ACE+, which is commercial multi-physical modeling software that has been proven to be accurate for plasma simulation. The simulated experimental results are analyzed to get a regression equation on three structural parameters. Through this equation, engineers can compute the uniformity of the electron number density rapidly without modeling by CFD-ACE+. An optimization performed at the end produces good results. 展开更多
关键词 fluid model inductively coupled plasma regression orthogonal structural parameters design of experiment
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Optimization of mechanical properties using D-optimal factorial design of experiment: Electromagnetic stir casting process of A357-SiC nanocomposite 被引量:2
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作者 Reza MOHAMMADI BADIZI Amir PARIZAD +1 位作者 Mohsen ASKARI-PAYKANI Hamid Reza SHAHVERDI 《Transactions of Nonferrous Metals Society of China》 SCIE EI CAS CSCD 2020年第5期1183-1194,共12页
Electromagnetic stir casting process of A357-Si C nanocomposite was discussed using the D-optimal design of experiment(DODOE) method. As the main objective, nine random experiments obtained by DX-7 software were perfo... Electromagnetic stir casting process of A357-Si C nanocomposite was discussed using the D-optimal design of experiment(DODOE) method. As the main objective, nine random experiments obtained by DX-7 software were performed. By this method, A357-Si C nanocomposites with 0.5, 1.0 and 1.5 wt.% Si C were fabricated at three different frequencies(10, 35 and 60 Hz) in the experimental stage. The microstructural evolution was characterized by scanning electron and optical microscopes, and the mechanical properties were investigated using hardness and roomtemperature uniaxial tensile tests. The results showed that the homogeneous distribution of Si C nanoparticles leads to the microstructure evolution from dendritic to non-dendritic form and a reduction of size by 73.9%. Additionally, based on DODOE, F-values of 44.80 and 179.64 were achieved for yield stress(YS) and ultimate tensile strength(UTS), respectively, implying that the model is significant and the variables(Si C fraction and stirring frequency) were appropriately selected. The optimum values of the Si C fraction and stirring frequency were found to be 1.5 wt.% and 60 Hz, respectively. In this case, YS and UTS for A357-Si C nanocomposites were obtained to be 120 and 188 MPa(57.7% and 57.9 % increase compared with those of the as-cast sample), respectively. 展开更多
关键词 A357 alloy electromagnetic stir casting Si C nanoparticles mechanical properties D-optimal design of experiments
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Design of experiment study on hardness variations in friction stir welding of AM60 Mg alloy 被引量:2
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作者 S.Richmire K.Hall M.Haghshenas 《Journal of Magnesium and Alloys》 SCIE EI CAS 2018年第3期215-228,共14页
Identification of process parameters,their effects and contributions to the outcomes of the system using experimental approach could be a daunting,time consuming,and costly course.Using proper statistical methods,i.e.... Identification of process parameters,their effects and contributions to the outcomes of the system using experimental approach could be a daunting,time consuming,and costly course.Using proper statistical methods,i.e.,Taguchi method,could significantly reduce the number of required experiments and statistical significance of the parameter can be identified.Friction stir welding is one of those welding techniques with many parameters which have different effects on the quality of the welds.In friction stir welding the tool rotational speed(RPM)and transverse speed(mm/min)influence the strength(i.e.,hardness distribution)of the stirred zone.In this study,these two factors are investigated to determine the effect they will have on the hardness in the stirred zone of the friction stir welds and how the two factors are related to one another for as-cast magnesium alloy AM60 with nominal chemical composition of Mg-(5.5-6.5)Al-(0.24-0.6)Mn-0.22Zn-0.1Si.Experimental data was taken at three different tool rotational speeds and three different transverse speeds.The data obtained was then analyzed using a 32 factorial design to find the contribution of these parameters.It was determined that both tool rotational speed and transverse speed possess significant effects on the stir zone hardness.Also,the interactions between the two factors were statistically assessed. 展开更多
关键词 AM60 Mg alloy design of experiment(DOE) Statistical method Friction stir welding
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Investigation of the optimum differential gear ratio for real driving cycles by experiment design and genetic algorithm 被引量:1
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作者 AHMED Aboud 赵长禄 张付军 《Journal of Beijing Institute of Technology》 EI CAS 2015年第1期65-73,共9页
Experiment statistical method and genetic algorithms based optimization method are used to obtain the optimum differential gear ratio for heavy truck that provides best fuel consumption when changing the working condi... Experiment statistical method and genetic algorithms based optimization method are used to obtain the optimum differential gear ratio for heavy truck that provides best fuel consumption when changing the working condition that affects its torque and speed range. The aim of the study is to obtain the optimum differential gear ratio with fast and accurate optimization calculation without affecting drivability characteristics of the vehicle according to certain driving cycles that represent the new working conditions of the truck. The study is carried on a mining dump truck YT3621 with 9 for- ward shift manual transmission. Two loading conditions, no load and 40 t, and four on road real driving cycles have been discussed. The truck powertrain is modeled using GT-drive, and DOE -post processing tool of the GT-suite is used for DOE analysis and genetic algorithm optimization. 展开更多
关键词 heavy trucks fuel consumption OPTIMIZATION design of experiment genetic algo-rithm differential gear ratio
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Computer modeling of high-pressure leaching process of nickel laterite by design of experiments and neural networks 被引量:1
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作者 Milovan Milivojevic Srecko Stopic +2 位作者 Bernd Friedrich Boban Stojanovic Dragoljub Drndarevic 《International Journal of Minerals,Metallurgy and Materials》 SCIE EI CAS CSCD 2012年第7期584-594,共11页
Due to the complex chemical composition of nickel ores, the requests for the decrease of production costs, and the increase of nickel extraction in the existing depletion of high-grade sulfide ores around the world, c... Due to the complex chemical composition of nickel ores, the requests for the decrease of production costs, and the increase of nickel extraction in the existing depletion of high-grade sulfide ores around the world, computer modeling of nickel ore leaching process be- came a need and a challenge. In this paper, the design of experiments (DOE) theory was used to determine the optimal experimental design plan matrix based on the D optimality criterion. In the high-pressure sulfuric acid leaching (HPSAL) process for nickel laterite in "Rudjinci" ore in Serbia, the temperature, the sulfuric acid to ore ratio, the stirring speed, and the leaching time as the predictor variables, and the degree of nickel extraction as the response have been considered. To model the process, the multiple linear regression (MLR) and response surface method (RSM), together with the two-level and four-factor full factorial central composite design (CCD) plan, were used. The proposed re- gression models have not been proven adequate. Therefore, the artificial neural network (ANN) approach with the same experimental plan was used in order to reduce operational costs, give a better modeling accuracy, and provide a more successful process optimization. The model is based on the multi-layer neural networks with the back-propagation (BP) learning algorithm and the bipolar sigmoid activation function. 展开更多
关键词 nickel laterite LEACHING computer simulation design of experiments (DOE) response surface method (RSM) neural networks
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Input signal design to estimate interwell connectivities in mature fields from the capacitance-resistance model 被引量:1
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作者 Gustavo A.Moreno Larry W.Lake 《Petroleum Science》 SCIE CAS CSCD 2014年第4期563-568,共6页
Interwell connectivities are fundamental parameters required to manage waterfloods in oil reservoirs. Data-driven models, such as the capacitance-resistance model (CRM), are fast tools to estimate these parameters f... Interwell connectivities are fundamental parameters required to manage waterfloods in oil reservoirs. Data-driven models, such as the capacitance-resistance model (CRM), are fast tools to estimate these parameters from time-correlations of input (injection rates) and output (production rates) signals. Noise and structure of the input time-series impose limits on the information that can be extracted from a given data-set. This work uses the CRM to study general prescriptions for the design of input signals that enhance the information content of injection/production data in the estimation of well-to-well interactions. Numerical schemes and general features of the optimal input signal strategy are derived for this problem. 展开更多
关键词 Intervell connectivity capacitance-resistance model mature field experiment design
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Optimization of DsbA Purification from Recombinant Escherichia coli Broth Using Box-Behnken Design Methodolog 被引量:1
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作者 LUO Man GUAN Yixin YAO Shanjing 《Chinese Journal of Chemical Engineering》 SCIE EI CAS CSCD 2013年第2期185-191,共7页
Disulfide bond formation protein A (DsbA) is one of the important helper proteins for folding in protein synthesis in vivo. In this study, purification of recombinant DsbA was investigated by examining four importan... Disulfide bond formation protein A (DsbA) is one of the important helper proteins for folding in protein synthesis in vivo. In this study, purification of recombinant DsbA was investigated by examining four important factors with Box-Behnken design method, a statistic-based design of experiments. The optimal operation conditions were obtained by adopting the effectiveness coefficient method on the multi-objective problem, which takes the protein recovery, purification efficiency and throughput of ion-exchange chromatography into account. After the optimization, protein recovery of 96.8% and purity higher than 95% DsbA was achieved, and the productivity was (377.9±1.7) mg soluble DsbA per liter broth. The purified protein was identified by peptide mass fingerprinting matching the record of gil2624856, a mutant of DsbA. The DsbA was preliminarily applied to the refolding of denatured lysozyme in vitro. 展开更多
关键词 disulfide bond formation protein A protein purification Box-Behnken experiment design response surface methodology multi-object programming
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Application of Monte-Carlo statistical experiments in design of ocean engineering - Estimating the parameters, models and probabilities 被引量:2
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作者 Liu Defu, Shi Jiangang and Zhou Zhigang Department of Ocean Engineering and Naval Architecture, Tianjin University. Tianjin, China Business & Project Division China Offshore Industrial Corporation (COIC).No. 10 Beixiaojie,Yuetan,BeijingsChina 《Acta Oceanologica Sinica》 SCIE CAS CSCD 1990年第4期587-597,共11页
Recently, some results have been acquired with the Monte- Carlo statistical experiments in the design of ocean en gineering. The results show that Monte-Carlo statistical experiments can be widely used in estimating t... Recently, some results have been acquired with the Monte- Carlo statistical experiments in the design of ocean en gineering. The results show that Monte-Carlo statistical experiments can be widely used in estimating the parameters of wave statistical distributions, checking the probability model of the long- term wave extreme value distribution under a typhoon condition and calculating the failure probability of the ocean platforms. 展开更多
关键词 Estimating the parameters Application of Monte-Carlo statistical experiments in design of ocean engineering
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