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Optimizing PID controller parameters for robust automatic voltage regulator system through indirect design approach-2
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作者 Mohd Zaidi Mohd Tumari Mohd Ashraf Ahmad +1 位作者 Mohd Riduwan Ghazali Mohd Helmi Suid 《Global Energy Interconnection》 EI CSCD 2024年第5期682-696,共15页
Automatic voltage regulators(AVR)are designed to manipulate a synchronous generator’s voltage level automatically.Proportional integral derivative(PID)controllers are typically used in AVR systems to regulate voltage... Automatic voltage regulators(AVR)are designed to manipulate a synchronous generator’s voltage level automatically.Proportional integral derivative(PID)controllers are typically used in AVR systems to regulate voltage.Although advanced PID tuning methods have been proposed,the actual voltage response differs from the theoretical predictions due to modeling errors and system uncertainties.This requires continuous fine tuning of the PID parameters.However,manual adjustment of these parameters can compromise the stability and robustness of the AVR system.This study focuses on the online self-tuning of PID controllers called indirect design approach-2(IDA-2)in AVR systems while preserving robustness.In particular,we indirectly tune the PID controller by shifting the frequency response.The new PID parameters depend on the frequency-shifting constant and the previously optimized PID parameters.Adjusting the frequency-shifting constant modifies all the PID parameters simultaneously,thereby improving the control performance and robustness.We evaluate the robustness of the proposed online PID tuning method by comparing the gain margins(GMs)and phase margins(PMs)with previously optimized PID parameters during parameter uncertainties.The proposed method is further evaluated in terms of disturbance rejection,measurement noise,and frequency response analysis during parameter uncertainty calculations against existing methods.Simulations show that the proposed method significantly improves the robustness of the controller in the AVR system.In summary,online self-tuning enables automated PID parameter adjustment in an AVR system,while maintaining stability and robustness. 展开更多
关键词 PID controller tuning AVR system Indirect design approach(IDA) Frequency-shifting Robust control
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Design approaches for active,semi-active and passive control systems based on analysis of characteristics of active control force 被引量:4
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作者 Ou Jinping Li Hui 《Earthquake Engineering and Engineering Vibration》 SCIE EI CSCD 2009年第4期493-506,共14页
In this paper, the characteristics of forces in active control systems connected to adjacent levels of a building are analyzed. The following characteristics are observed: (1) active control can provide significant... In this paper, the characteristics of forces in active control systems connected to adjacent levels of a building are analyzed. The following characteristics are observed: (1) active control can provide significantly superior supplemental damping to a building, but causes a small frequency shift; (2) the linear quadratic regulator (LQR)-based control force is composed of an elastic restoring force component and a damping force component, where the damping force is almost identical to the total control force, however, the elastic restoring force is very small; and (3) the active control forces prevent mction most of the time during the entire control process. These three characteristics imply that active control systems connected to adjacent levels of a building behave like passive damping devices with adjustable parameters, namely damping characteristics in an active control, which is the mechanism used by semi-active control devices to reach similar performance as active control systems. Two indices are defined to quantify the damping characteristics of control forces in active control systems. These two indices can also be used to quantify the capacity of semi-active control to achieve the perfonrlance of active control. Based on the above observations, two principles are founded for optimization of parameters of semi-active control devices and passive dampers. The first is that the maximum output force of a semi-active or passive device to be designed is identical to an active device, called "design principle". The other is the response equivalent principle, which states that the response of a building with semi-active or passive devices is the same as with active devices when the same maximum output force is applied. The design procedure for semi-active control devices and passive dampers is described in detail. Finally, numerical simulations of two benchmark problems is conducted to demonstrate the damping characteristics of active control and investigate the capacity of semi-active control to achieve the same performance as active control. 展开更多
关键词 active control: semi-active control smart control DAMPING design approach
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Development and in-vitro evaluation of an optimized carvedilol transdermal therapeutic system using experimental design approach
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作者 Namrata Vora S.Lin P.L.Madan 《Asian Journal of Pharmaceutical Sciences》 SCIE CAS 2013年第1期28-38,共11页
The effect of formulation variables on in-vitro release and permeation properties of carvedilol from transdermal patch was studied by varying one factor at a time as preliminary study.Based on these results,design of ... The effect of formulation variables on in-vitro release and permeation properties of carvedilol from transdermal patch was studied by varying one factor at a time as preliminary study.Based on these results,design of experiments technique was applied followed by regression analysis and response surface methodology to optimize formulation variables.Central Composite IV model design was used with four formulation variables:drug loading,matrix thickness,adhesive layer thickness,and propylene glycol concentration.Nineteen formulations were prepared according to the design;and the effect of formulation variables was studied on in-vitro release and permeation profiles of these formulations.In all cases,the permeation profiles paralleled in-vitro release profiles.The drug released at 7 h and 24 h was used as release response parameters while permeation flux obtained was employed as permeation response parameter.All four formulation variables were found to be significant for release properties and three of these exhibited significant effect on permeation profile of carvedilol across artificial membrane.Constrained optimization,using 47.9%of cumulative carvedilol released at 7 h and 99.8%at 24 h as well as 25.7 mg/cm2/h of permeation flux,was applied to obtain desired release and permeation profiles.Experimentally,carvedilol was observed to release from the optimized formulation with 51.4%drug release at 7 h and 98.5%at 24 h with an observed flux value of 27.4 mg/cm2/h across artificial membrane,which showed an excellent agreement with the predicted values.The results of this investigation show that the quadratic mathematical model developed could be used to further predict formulations with desirable release and permeation properties. 展开更多
关键词 TRANSDERMAL CARVEDILOL Controlled release Experimental design approach
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Constructing Optimal CAE Design Approach Model: Application of the Advanced TDS 被引量:1
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作者 Kakuro Amasaka 《通讯和计算机(中英文版)》 2012年第7期774-786,共13页
关键词 设计方法 TDS CAE 产品型号 应用 设计模式 设计时间 金属颗粒
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Generalized Odd-Even Mode Theory and Mode Synthesis Antenna Design Approach
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作者 Wen-Jun Lu 《Electromagnetic Science》 2024年第1期20-37,共18页
In this article,studies on the multimode excitation problem of waveguides and antennas,the balance/unbalance mech-anism and the balanced feeding techniques in dipole antenna systems are first briefly historically revi... In this article,studies on the multimode excitation problem of waveguides and antennas,the balance/unbalance mech-anism and the balanced feeding techniques in dipole antenna systems are first briefly historically reviewed.In this context,general-ized odd-even mode theory is advanced to quantitatively and approximately describe the mutual coupling effect between a feed line and an antenna.As is mathematically deduced and demonstrated,the modal parity mismatch between the feed line and the antenna should ultimately dominate the unbalance phenomenon in antenna systems.Thus,an elegant,closed-form formula is derived to ap-proximately calculate the“unbalance degree”of a straight dipole off-center fed by a symmetric twin-wire line.Design approaches for the simplest,linear,1-D multimode resonant antennas are introduced.Moreover,the“falling tone excitation”law gauged based on prototype dipoles is revealed and used to develop a mode synthesis design approach for microstrip patch antennas(MPAs)and 2-D sectorial electric dipole antennas.Design examples with distinctive radiation performance are presented and discussed.Finally,possible development trends of multimode resonant antennas are prospected. 展开更多
关键词 Generalized odd-even mode theory Modal parity mismatch Mode synthesis antenna design approach Multimode resonant antenna
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Experimental Design of Measuring Soil-Water Characteristic Curve of Unsaturated Soil Using Bayesian Approach
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作者 Shaolin Ding 《World Journal of Engineering and Technology》 2024年第4期996-1007,共12页
Soil-water characteristic curve (SWCC) is significant to estimate the site-specific unsaturated soil properties (such as unsaturated shear strength and coefficient of permeability) for geotechnical analyses involving ... Soil-water characteristic curve (SWCC) is significant to estimate the site-specific unsaturated soil properties (such as unsaturated shear strength and coefficient of permeability) for geotechnical analyses involving unsaturated soils. Determining SWCC can be achieved by fitting data points obtained according to the prescribed experimental scheme, which is specified by the number of measuring points and their corresponding values of the control variable. The number of measuring points is limited since direct measurement of SWCC is often costly and time-consuming. Based on the limited number of measuring points, the estimated SWCC is unavoidably associated with uncertainties, which depends on measurement data obtained from the prescribed experimental scheme. Therefore, it is essential to plan the experimental scheme so as to reduce the uncertainty in the estimated SWCC. This study presented a Bayesian approach, called OBEDO, for probabilistic experimental design optimization of measuring SWCC based on the prior knowledge and information of testing apparatus. The uncertainty in estimated SWCC is quantified and the optimal experimental scheme with the maximum expected utility is determined by Subset Simulation optimization (SSO) in candidate experimental scheme space. The proposed approach is illustrated using an experimental design example given prior knowledge and the information of testing apparatus and is verified based on a set of real loess SWCC data, which were used to generate random experimental schemes to mimic the arbitrary arrangement of measuring points during SWCC testing in practice. Results show that the arbitrary arrangement of measuring points of SWCC testing is hardly superior to the optimal scheme obtained from OBEDO in terms of the expected utility. The proposed OBEDO approach provides a rational tool to optimize the arrangement of measuring points of SWCC test so as to obtain SWCC measurement data with relatively high expected utility for uncertainty reduction. 展开更多
关键词 Bayesian approach Subset Simulation Optimization Probabilistic Experiment design SWCC Expected Utility
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Assessing the Applicability of Three Approaches to Design-Oriented Research
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作者 Jing Zhou Christopher Kueh Yi Lin 《Journal of Contemporary Educational Research》 2021年第8期36-40,共5页
The three main approaches in inquisitive research design are qualitative,quantitative,and mixed methods[1].However,recent developments in the research field have resulted in multiple other approaches,borrowing ideas f... The three main approaches in inquisitive research design are qualitative,quantitative,and mixed methods[1].However,recent developments in the research field have resulted in multiple other approaches,borrowing ideas from a broad range of fields.One such approach is the practice-led approach.This approach involves an efficient design process,novel qualitative interviewing methods,together with data mining procedures from quantitative data collection[2].This paper assesses the practice-led approach used in user experience(UX)design,together with three approaches:co-design,service design,and reflective practice. 展开更多
关键词 Practice-led approach design approaches User experience
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Fault reconstruction observer design for continuous-time systems with measurement disturbances via descriptor system approach 被引量:1
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作者 Qingxian Jia Yingchun Zhang +1 位作者 Chengliang Li Xueqin Chen 《Journal of Systems Engineering and Electronics》 SCIE EI CSCD 2014年第5期877-885,共9页
This paper addresses a problem of observer-based sensor fault reconstruction for continuous-time systems subject to sensor faults and measurement disturbances via a descriptor system approach. An augmented descriptor ... This paper addresses a problem of observer-based sensor fault reconstruction for continuous-time systems subject to sensor faults and measurement disturbances via a descriptor system approach. An augmented descriptor plant is first formulated, by assembling measurement disturbances and sensor faults into an auxiliary state vector. Then a novel descriptor state observer for the augmented plant is constructed such that simultaneous reconstruction of original system states, sensor faults and measurement disturbances are obtained readily. Sufficient conditions for the existence of the proposed observer are explicitly provided, and the application scope of the observer is further discussed. In addition, an extension of the proposed linear approach to a class of nonlinear systems with Lipschitz constraints is investigated. Finally, two numerical examples are simulated to illustrate the effectiveness of the proposed fault-reconstructing approaches. 展开更多
关键词 fault reconstruction descriptor system approach ob- server design measurement disturbance.
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A Trace-state Based Approach to Specification and Design of Parallel Programs
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作者 He Jifeng Oxford University Computing LaboratoryProgramming Research Group Parks Road, Oxford OXl 3QD, England 《计算机工程》 CAS CSCD 北大核心 1996年第S1期91-105,共15页
In this paper they deal with the issue of specification and design of parallel communicatingprocesses. A trace-state based model is introduced to describe the behaviour of concurrent programs. They presenta formal sys... In this paper they deal with the issue of specification and design of parallel communicatingprocesses. A trace-state based model is introduced to describe the behaviour of concurrent programs. They presenta formal system based on that model to achieve hierarchical and modular development and verification methods. Anumber of refinement rules are used to decompose the specification into smaller ones and calculate program fromthe 展开更多
关键词 COMM A Trace-state Based approach to Specification and design of Parallel Programs
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Product Family Design Methodology Using Object-Oriented Approach
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作者 WANG Ai-min, MENG Ming-chen, HUANG Jing-yuan (Department. of Precision Instruments and Mechanology, Tsinghua Univers ity, Beijing 100084, China) 《厦门大学学报(自然科学版)》 CAS CSCD 北大核心 2002年第S1期141-142,共2页
The serious competition environment of global marke t requests the enterprise to change traditional product development strategy and a dopt new theory in order to meet diverse customer needs while maintaining near m a... The serious competition environment of global marke t requests the enterprise to change traditional product development strategy and a dopt new theory in order to meet diverse customer needs while maintaining near m ass production efficiency, which is the main philosophy of mass customization. P roduct family design is research focus at present and also is the core technolog y of DFMC (design for mass customization). Firstly, this paper explores the fund amental issues of product family, such as concepts of modularity, commonality/di versity, product platform and product family architecture etc. We compare the te rminology between product family and object-oriented approach in the next step. Thirdly, this paper puts forwards one product family design methodology based o n product platform and under different phase of product life cycle constrains, f or example, functional, assembly and service etc. At the end section of this pap er, we applied, the object-oriented approach in above mentioned product family design methodology to realize the design process. In one word, this paper propos ed one product family design methodology based on object oriented approach and p roduct life cycle consideration, especially the conjointness of characteristic o f OOA and concepts of product family. The main property of OOA are encapsulation , inherence and polymorphism. Encapsulation can represent the module or building blocks of product family. Inherence can be extended to describe the modularity and commonality, and also be used to construct variant space. Alternative specif ic of product family architecture can be embodied with polymorphism. And fin aly, we give the future work contents. In order to derive the product platform a nd achieve modularity and commonality/diversity, interface management between bu ilding block is necessary. The question is how the OOA can be applied in interfa ce management to get our aim OOA is the basis of many information management sy stem, then the question is how to build one system to manage the information of product family and support mass customization The third question is how to deve lop one computer aided tool to facilitate the application of OOA for product fam ily design, even be used to category of design for mass customization. 展开更多
关键词 product family design product platform object- oriented approach (OOA) product life cycle
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Application of the Robust Approach and Finite Element Analysis in Door System Static Design
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作者 LI Chao ZHONG Zhi-hua ZHU Wu 《International Journal of Plant Engineering and Management》 2010年第2期111-116,共6页
The robust design approach based on Taguchi's design of experiment (DOE) coupled with the traditional finite element analysis (FEA) is applied to the static design of a sedan door. This method aims at shortening ... The robust design approach based on Taguchi's design of experiment (DOE) coupled with the traditional finite element analysis (FEA) is applied to the static design of a sedan door. This method aims at shortening the design period, reducing the calculation cost and improving the competitive competence. First, the pe^ormance re- quirements, the control factors and the corresponding levels are determined to design a DOE table. Second, based on the schemes from the DOE table, sensitive control factors are distinguished from the results of the FEA method. Finally, the optimum design is chosen after comparisons and analyses of these performance response graphs. Performance results of the optimum design indicate that the application of the robust approach and FEA can greatly im- prove the quality of the door system, satisfy the requirements of products, underline the design purpose and shorten the design period. 展开更多
关键词 robust approach Tagnchi's design door system DOE FEA
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Airfoil design optimization based on lattice Boltzmann method and adjoint approach
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作者 Xiaowei LI Liang FANG Yan PENG 《Applied Mathematics and Mechanics(English Edition)》 SCIE EI CSCD 2018年第6期891-904,共14页
We present a new aerodynamic design method based on the lattice Boltzmann method (LBM) and the adjoint approach. The flow field and the adjoint equation are numerically simulated by the GILBM (generalized form of i... We present a new aerodynamic design method based on the lattice Boltzmann method (LBM) and the adjoint approach. The flow field and the adjoint equation are numerically simulated by the GILBM (generalized form of interpolation supplemented LBM) on non-uniform meshes. The first-order approximation for the equilibrium dis- tribution function on the boundary is proposed to diminish the singularity of boundary conditions. Further, a new treatment of the solid boundary in the LBM is described par- ticularly for the airfoil optimization design problem. For a given objective function, the adjoint equation and its boundary conditions are derived analytically. The feasibility and accuracy of the new approach have been perfectly validated by the design optimization of NACA0012 airfoil. 展开更多
关键词 lattice Boltzmann method (LBM) adjoint approach boundary treatment aerodynamic design optimization
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An Innovative Soft Design Science Methodology for Improving Development of a Secure Information System in Tanzania Using Multi-Layered Approach 被引量:2
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作者 Maduhu Mshangi Edephonce Ngemera Nfuka Camilius Sanga 《Journal of Information Security》 2017年第3期141-165,共25页
This paper presents an innovative Soft Design Science Methodology for improving information systems security using multi-layered security approach. The study applied Soft Design Science Methodology to address the prob... This paper presents an innovative Soft Design Science Methodology for improving information systems security using multi-layered security approach. The study applied Soft Design Science Methodology to address the problematic situation on how information systems security can be improved. In addition, Soft Design Science Methodology was compounded with mixed research methodology. This holistic approach helped for research methodology triangulation. The study assessed security requirements and developed a framework for improving information systems security. The study carried out maturity level assessment to determine security status quo in the education sector in Tanzania. The study identified security requirements gap (IT security controls, IT security measures) using ISO/IEC 21827: Systems Security Engineering-Capability Maturity Model (SSE-CMM) with a rating scale of 0 - 5. The results of this study show that maturity level across security domain is 0.44 out of 5. The finding shows that the implementation of IT security controls and security measures for ensuring security goals are lacking or conducted in ad-hoc. Thus, for improving the security of information systems, organisations should implement security controls and security measures in each security domain (multi-layer security). This research provides a framework for enhancing information systems security during capturing, processing, storage and transmission of information. This research has several practical contributions. Firstly, it contributes to the body of knowledge of information systems security by providing a set of security requirements for ensuring information systems security. Secondly, it contributes empirical evidence on how information systems security can be improved. Thirdly, it contributes on the applicability of Soft Design Science Methodology on addressing the problematic situation in information systems security. The research findings can be used by decision makers and lawmakers to improve existing cyber security laws, and enact laws for data privacy and sharing of open data. 展开更多
关键词 SOFT design SCIENCE Information SYSTEMS Security design SCIENCE Research SOFT SYSTEMS METHODOLOGY Multi-Layered approach
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A Neural Network Approach for Designing 2-D FIR Filters with Arbitrary Magnitude Responses
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作者 Xiaohua Wang Yigang He 《通讯和计算机(中英文版)》 2006年第3期66-71,共6页
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Meditation on 2D and 3D Approach in Computer Aided Shape Design
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《Computer Aided Drafting,Design and Manufacturing》 2000年第2期45-49,共5页
关键词 Meditation on 2D and 3D approach in Computer Aided Shape design AEC CAD
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Design of Pastoral Cultural Landscape Oriented by the Concept of Ecology Villages 被引量:2
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作者 陈晓刚 刘萍 《Agricultural Science & Technology》 CAS 2017年第2期314-317,共4页
China is increasingly attaching importance to coordination and scientific development between humanist and economic constructions, especially for protection on rural pastoral cultural landscape. For eco-village theory... China is increasingly attaching importance to coordination and scientific development between humanist and economic constructions, especially for protection on rural pastoral cultural landscape. For eco-village theory, planning and construction of pastoral culture landscape is just a breakthrough. The research used modern landscaping technology and analyzed pastoral landscape culture, facilitating transformation of agricultural production pattern and ushering in a new trend in terms of retaining rural cultural landscapes with Chinese characteristics and inheriting and innovating rural culture. 展开更多
关键词 Ecological village Pastoral cultural landscape designing approach
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Influence of Waterside Buildings’ Layout on Wind Environment and the Relation with Design Based on a Case Study of the She Kou Residential District 被引量:1
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作者 Lei Yu Yongyi Ye Lu Zhang 《Journal of Architectural Environment & Structural Engineering Research》 2021年第3期20-30,共11页
It is important to improve residential thermal comfort in the high dense cities,in which wind environment is crucial.Waterside buildings take an advantage of micro-hydrological-climate in summer that should be used to... It is important to improve residential thermal comfort in the high dense cities,in which wind environment is crucial.Waterside buildings take an advantage of micro-hydrological-climate in summer that should be used to enhance residential thermal comfort especially in the subtropical region.In order to propose design approaches according to the outdoor thermal comfort of the waterside residential,a case study of Shenzhen She Kou res­idential district has been made.It focused on various factors that could have influence on wind environment for improving thermal comfort.Using wind velocity ratio(ΔRi)criterion,factors of building development volume,building direction and layout pattern,open space arrangement etc.have been broadly explored using FLUENT simulation.To planning parameters,the Floor Area Ratio(FAR)is significantly influence wind environment,the smaller FAR is better.To the vertical layout of the buildings,multi-storey layout and multi-storey&sub high-rise mixed layout would provide better wind environment.To the horizontal layout,the determinant is better than the peripheral.Other factors such as the buildings’direction towards the road,buildings’height,and open space setting,have influence on wind environment yet.In general,the more benefit of design layout for wind breezing,the better wind environment it could get. 展开更多
关键词 Buildings’layout Waterside Wind environment design approach
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内镜下经下颌角-颈静脉孔区手术入路的解剖学研究
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作者 崔润台 高永峰 +1 位作者 梁智星 刘军 《局解手术学杂志》 2025年第2期95-98,共4页
目的探讨内镜下经下颌角-颈静脉孔区手术入路的解剖学基础。方法收集湿性尸头标本5具,模拟内镜下经下颌角-颈静脉孔区手术入路对湿性尸头标本进行解剖,观察相关解剖结构,测量手术操作深度和角度。结果该手术入路形成了带蒂下颌角骨瓣,... 目的探讨内镜下经下颌角-颈静脉孔区手术入路的解剖学基础。方法收集湿性尸头标本5具,模拟内镜下经下颌角-颈静脉孔区手术入路对湿性尸头标本进行解剖,观察相关解剖结构,测量手术操作深度和角度。结果该手术入路形成了带蒂下颌角骨瓣,骨瓣长为(23.74±0.95)mm,宽为(18.95±0.56)mm。移位下颌角骨瓣后手术操作深度为(8.54±0.55)mm,复位下颌角骨瓣后手术操作深度为(24.94±0.90)mm,差异有统计学意义(P<0.05);移位下颌角骨瓣后手术操作角度为(69.60±3.30)°,复位下颌角骨瓣后手术操作角度为(26.20±2.20)°,差异有统计学意义(P<0.05)。结论内镜下经下颌角-颈静脉孔区手术入路形成带蒂下颌角骨瓣,可以增加手术操作角度、缩短操作深度,提高手术自由度,该手术入路值得进一步研究和在临床实践中探讨。 展开更多
关键词 下颌角 颈静脉孔 解剖学 内镜手术 手术入路设计
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DIRECT DIGITAL DESIGN AND SIMULATION OF MESHING IN WORM-GEAR DRIVE 被引量:10
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作者 XU Wujiao QIN Datong SHI Wankai 《Chinese Journal of Mechanical Engineering》 SCIE EI CAS CSCD 2006年第3期428-433,共6页
A direct digital design method (DDDM) of worm-gear drive is proposed. It is directly based on the simulation of manufacturing process and completely different from the conventional modeling method. The loaded tooth ... A direct digital design method (DDDM) of worm-gear drive is proposed. It is directly based on the simulation of manufacturing process and completely different from the conventional modeling method. The loaded tooth contact analysis (LTCA) method is analyzed, in which the advanced surface to surface searching technique is included. The influence of misalignment errors and contact deformations on contact zone and transmission error (TE) is discussed. Combined modification approach on worm tooth surface is presented. By means of DDDM and LTCA, it is very conven- ient to verify the effect of worm-gear drive's modification approach. The analysis results show that, the modification in profile direction reduces the sensitivity of worm-gear drive to misalignment errors and the modification in longitudinal direction decreases the TE. Thus the optimization design of worm-gear drive can be achieved prior to the actual manufacturing process. 展开更多
关键词 Direct digital design Loaded tooth contact analysis Combined modification approach Worm-gear drive
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Optimization of Fermentation Medium for Epothilones Production with Sequential Statistical Approach 被引量:6
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作者 MENG Fan-xin LI You-xin GUO Wei-liang LU Jia-hui DU Lin-na TENG Li-rong 《Chemical Research in Chinese Universities》 SCIE CAS CSCD 2010年第1期86-91,共6页
A sequential statistical approach was applied to optimizing the fermentation medium of epothilones(Epos) production by means of a mutant which was obtained by treating polyangium cellulosum ATCC 15384 with nitrite a... A sequential statistical approach was applied to optimizing the fermentation medium of epothilones(Epos) production by means of a mutant which was obtained by treating polyangium cellulosum ATCC 15384 with nitrite and ultraviolet. The effects of different carbon sources and nitrogen sources on the fermentation medium were tested, and the suitable ones were selected. Then a uniform design was employed to design the experiments. A linear model was developed for identifying the significant components in fermentation medium, while a third degree polynomial model was used for studying the relationship between the concentration of the components in fermentation medium and the yield of Epos(YEPs). A pattern search method was used for searching the optimum fermentation medium in the test space, which was as follows(g/L): potassium nitrate 8.00, soybean peptone 17.60, potassium hydrogen phos- phate 1.00, beef extraction 6.46, yeast extraction 1.00, calcium chloride 0.25, sodium chloride 1.00 and ferric chloride 0.02. The optimum fermentation medium was expected to result in a yield of Epos(YEPs) of 2.48 mg/L. The validation experiments with the optimum medium were performed in triplicate and the average yield of Epos was 2.45 mg/L which was 7.78 times higher than that of Epos prepared without optimization. 展开更多
关键词 Statistical approach EPOTHILONES Uniform design Optimization of fermentation medium
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