Objective:To explore the effect of naringin on hippocampus estrogen receptor and neuronal cell apoptosis in a mouse model of menopausal depression.Methods:The menopausal depression model was replicated by bilateral ov...Objective:To explore the effect of naringin on hippocampus estrogen receptor and neuronal cell apoptosis in a mouse model of menopausal depression.Methods:The menopausal depression model was replicated by bilateral ovariectomy(OVX)combined with chronic unpredictable mild stimulation(CUMS),54 female Kunming mice were randomly divided into Blank group,Model group(OVX+CUMS group),Sham operation group(SHAM group),Estradiol group(E2 group 20.090 mg∙Kg-1∙d-1),Naringin group(100 mg∙Kg-1∙d-1),Naringin+ICI182780 group(100 mg∙Kg-1∙d-1+0.075 mg∙Kg-1∙d-1),each group of 9 mice were administered for 21 consecutive days.The depression-like behaviour changes of each group of mice were observed by tail suspension and forced swimming experiments;The morphological changes of hippocampal neurons were observed by HE staining;Immunohistochemical method was used to detect the expression of ERαand ERβpositive cells in the hippocampus of mice;The expression of ERβ,GluR2,CAMK栻,NMDAR1,Bad,Bcl-2 and Caspase3 proteins in the hippocampus of the mice was detected by western blot.Results:Compared with the Blank group,the immobility time of the mice in the OVX+CUMS group was significantly increased(P<0.01),mice hippocampal neurons with a sparsely organized cell arrangement,nuclear fixation,the expressions of ERβ,GluR2,and Bcl-2 were decreased(P<0.01,P<0.001),and the expressions of NMDAR1,CAMK栻,Bad,and Caspase3 were increased(P<0.05,P<0.01);Compared with the OVX+CUMS group,the immobility time of the mice in the SHAM group,E2 group and Naringin group was significantly reduced(P<0.01),mice hippocampal neuronal cells are relatively intact and tightly packed,the expressions of ERβ,GluR2 and Bcl-2 were increased(P<0.01,P<0.05),and the expressions of NMDAR1,CAMK栻,Bad and Caspase3 were decreased(P<0.05,P<0.01);Compared with the Naringin group,the quiescent time of the mice in the Naringin+ICI182780 group was increased(P<0.05),the smaller cell body of mouse hippocampal neurons,the expressions of ERβ,GluR2,and Bcl-2 were decreased(P<0.01,P>0.05,P<0.05),and the expressions of NMDAR1,CAMK栻,Bad,and Caspase3 were increased(P<0.01,P<0.05).Conclusions:Naringin may reduce neuronal cell excitotoxicity caused by glutamate receptor activation,reduce neuronal cell apoptosis and improve depression-like behavior by activating ERβreceptors in the hippocampus in a mouse model of menopause depression.展开更多
Despair is defined in the dictionary as the complete loss or absence of hope. Many if not most individuals feel a deep sense of despair, made more intense by the loneliness that is part of their lives. The power diffe...Despair is defined in the dictionary as the complete loss or absence of hope. Many if not most individuals feel a deep sense of despair, made more intense by the loneliness that is part of their lives. The power differentials between those who receive services and those who provide services often intensify this sense of despair and loneliness. It is in that relationship between the powerful and the powerless that loneliness and despair start to build, because there is no bridge between the people who serve and those being served. Those of us who provide services and supports to people in need have been taught to be objective and factual, but facts are only part of the story, the roadway so to speak. The foundations of the bridges between people are found in the emotional connections, the places where the stories of their lives take shape. In order to gain the whole story we must listen to the feelings, where the thematic images behind the words present the story of this person’s life.展开更多
The commentary on A Clean, well-lighted Place intends to release general introduction about Hemingway's short story, especially his writing styles through a few short conversations between the young and middle-age...The commentary on A Clean, well-lighted Place intends to release general introduction about Hemingway's short story, especially his writing styles through a few short conversations between the young and middle-aged waiters to evince the value of life in simple words by Hemingway.展开更多
This thesis is to analyze Hemingway's 'A Clean,Well-lighted Place'-a story to which the author applies his famous 'iceberg theory'.Hemingway relates his stories to an iceberg with only one-eighth a...This thesis is to analyze Hemingway's 'A Clean,Well-lighted Place'-a story to which the author applies his famous 'iceberg theory'.Hemingway relates his stories to an iceberg with only one-eighth actually showing and the other seven-eighths under water.This thesis is to show what is beneath the surface and the hidden meaning of the story.展开更多
基金Research Project of Wuxi Municipal Health Commission (No.M202206)。
文摘Objective:To explore the effect of naringin on hippocampus estrogen receptor and neuronal cell apoptosis in a mouse model of menopausal depression.Methods:The menopausal depression model was replicated by bilateral ovariectomy(OVX)combined with chronic unpredictable mild stimulation(CUMS),54 female Kunming mice were randomly divided into Blank group,Model group(OVX+CUMS group),Sham operation group(SHAM group),Estradiol group(E2 group 20.090 mg∙Kg-1∙d-1),Naringin group(100 mg∙Kg-1∙d-1),Naringin+ICI182780 group(100 mg∙Kg-1∙d-1+0.075 mg∙Kg-1∙d-1),each group of 9 mice were administered for 21 consecutive days.The depression-like behaviour changes of each group of mice were observed by tail suspension and forced swimming experiments;The morphological changes of hippocampal neurons were observed by HE staining;Immunohistochemical method was used to detect the expression of ERαand ERβpositive cells in the hippocampus of mice;The expression of ERβ,GluR2,CAMK栻,NMDAR1,Bad,Bcl-2 and Caspase3 proteins in the hippocampus of the mice was detected by western blot.Results:Compared with the Blank group,the immobility time of the mice in the OVX+CUMS group was significantly increased(P<0.01),mice hippocampal neurons with a sparsely organized cell arrangement,nuclear fixation,the expressions of ERβ,GluR2,and Bcl-2 were decreased(P<0.01,P<0.001),and the expressions of NMDAR1,CAMK栻,Bad,and Caspase3 were increased(P<0.05,P<0.01);Compared with the OVX+CUMS group,the immobility time of the mice in the SHAM group,E2 group and Naringin group was significantly reduced(P<0.01),mice hippocampal neuronal cells are relatively intact and tightly packed,the expressions of ERβ,GluR2 and Bcl-2 were increased(P<0.01,P<0.05),and the expressions of NMDAR1,CAMK栻,Bad and Caspase3 were decreased(P<0.05,P<0.01);Compared with the Naringin group,the quiescent time of the mice in the Naringin+ICI182780 group was increased(P<0.05),the smaller cell body of mouse hippocampal neurons,the expressions of ERβ,GluR2,and Bcl-2 were decreased(P<0.01,P>0.05,P<0.05),and the expressions of NMDAR1,CAMK栻,Bad,and Caspase3 were increased(P<0.01,P<0.05).Conclusions:Naringin may reduce neuronal cell excitotoxicity caused by glutamate receptor activation,reduce neuronal cell apoptosis and improve depression-like behavior by activating ERβreceptors in the hippocampus in a mouse model of menopause depression.
文摘Despair is defined in the dictionary as the complete loss or absence of hope. Many if not most individuals feel a deep sense of despair, made more intense by the loneliness that is part of their lives. The power differentials between those who receive services and those who provide services often intensify this sense of despair and loneliness. It is in that relationship between the powerful and the powerless that loneliness and despair start to build, because there is no bridge between the people who serve and those being served. Those of us who provide services and supports to people in need have been taught to be objective and factual, but facts are only part of the story, the roadway so to speak. The foundations of the bridges between people are found in the emotional connections, the places where the stories of their lives take shape. In order to gain the whole story we must listen to the feelings, where the thematic images behind the words present the story of this person’s life.
文摘The commentary on A Clean, well-lighted Place intends to release general introduction about Hemingway's short story, especially his writing styles through a few short conversations between the young and middle-aged waiters to evince the value of life in simple words by Hemingway.
文摘This thesis is to analyze Hemingway's 'A Clean,Well-lighted Place'-a story to which the author applies his famous 'iceberg theory'.Hemingway relates his stories to an iceberg with only one-eighth actually showing and the other seven-eighths under water.This thesis is to show what is beneath the surface and the hidden meaning of the story.
