To further understand characters of alkaline phosphatase and provide reference for in-depth study of the mechanism of NFRKN-1 invading into knot nem-atode and its development and utilization, the effects of metal ions...To further understand characters of alkaline phosphatase and provide reference for in-depth study of the mechanism of NFRKN-1 invading into knot nem-atode and its development and utilization, the effects of metal ions, organic reagents and chemical modifier on activity of alkaline phosphatase from DFRKN-1 were analyzed in the paper. The results showed that Mg^2＋ , Ba^2＋ and K^＋ under certain concentrations activated enzyme significantly and Zn^2＋ could inhibit enzyme activ-ity. Methanol, ethanol and ethylene glycol inhibited enzyme activity in similar degrees. Histidine residue and Lysine residue are essential groups of alkaline pbos-phatase; sulflaydryl of cysteine was not an essential group, but disulfide bond played an important role in maintaining the active conformation of alkaline phospha-tase.展开更多
基金Supported by Key Laboratory Project of State Ethnic Affairs Commission (2010SY12)
文摘To further understand characters of alkaline phosphatase and provide reference for in-depth study of the mechanism of NFRKN-1 invading into knot nem-atode and its development and utilization, the effects of metal ions, organic reagents and chemical modifier on activity of alkaline phosphatase from DFRKN-1 were analyzed in the paper. The results showed that Mg^2＋ , Ba^2＋ and K^＋ under certain concentrations activated enzyme significantly and Zn^2＋ could inhibit enzyme activ-ity. Methanol, ethanol and ethylene glycol inhibited enzyme activity in similar degrees. Histidine residue and Lysine residue are essential groups of alkaline pbos-phatase; sulflaydryl of cysteine was not an essential group, but disulfide bond played an important role in maintaining the active conformation of alkaline phospha-tase.
