Atrazine(AT,2-chloro-4-ethylamino-6-isopropyl-amino-s-triazine)has been detected in ground water in several areas of the United States for many years,as well as in China,wherein the growth rate of its gross
A set of universal loop-mediated isothermal amplification (LAMP) primers targeting the flo gene was designed to detect Borrelia burgdorferi sensu lato (B. burgdorferi s.I.) in human samples. The sensitivity of LAM...A set of universal loop-mediated isothermal amplification (LAMP) primers targeting the flo gene was designed to detect Borrelia burgdorferi sensu lato (B. burgdorferi s.I.) in human samples. The sensitivity of LAMP was 20 copies/reaction, and the assay did not detect false positives among 11 other related bacteria. A positive LAMP result was obtained for 9 of the 24 confirmed cases and for 12 of 94 suspected cases. The positive rate of LAMP was the same as that of nested PCR. The LAMP is a useful diagnostic method that can be developed for rapid detection of B. burgdorferi s.I. in human sera. Combination of the LAMP and nested PCR was more sensitive for detecting B. burgdorferi s.I. in human serum samples.展开更多
Owing to the excellent stability,biocompatibility and photoluminescence property,graphene quantum dots(GQDs)are emerging as a kind of potential materials to be applied in a series of fields ranging from sensor to drug...Owing to the excellent stability,biocompatibility and photoluminescence property,graphene quantum dots(GQDs)are emerging as a kind of potential materials to be applied in a series of fields ranging from sensor to drug delivery.As the growing concern for human and environmental safety,selective detection of metal ions has been paid more and more attention.GQDs,as nanoparticles with superior optical properties,have been attracting growing attention in the field of metal ions detection.In this work,glutathione(GSH)functionalized boron doped graphene quantum dots(B-GQDs@GSH)were successfully synthesized with stable bright blue fluorescence and has been used for the detection of Fe^(3+).A good linear relationship between 1/(F_(0)-F)and 1/c with the concentration ranging from 0.70 to 53μmol/L was obtained with a detection limit of 5.5 nmol/L.The application of B-GQDs@GSH for Fe^(3+)detection in water samples was demonstrated and the quenching mechanism was further explored.Due to low cytotoxicity and favorable biocompatibility,B-GQDs@GSH were successfully applied for cell fluorescence imaging and intracellular determination of Fe^(3+).展开更多
Recently,self-supervised learning has shown great potential in Graph Neural Networks (GNNs) through contrastive learning,which aims to learn discriminative features for each node without label information. The key to ...Recently,self-supervised learning has shown great potential in Graph Neural Networks (GNNs) through contrastive learning,which aims to learn discriminative features for each node without label information. The key to graph contrastive learning is data augmentation. The anchor node regards its augmented samples as positive samples,and the rest of the samples are regarded as negative samples,some of which may be positive samples. We call these mislabeled samples as “false negative” samples,which will seriously affect the final learning effect. Since such semantically similar samples are ubiquitous in the graph,the problem of false negative samples is very significant. To address this issue,the paper proposes a novel model,False negative sample Detection for Graph Contrastive Learning (FD4GCL),which uses attribute and structure-aware to detect false negative samples. Experimental results on seven datasets show that FD4GCL outperforms the state-of-the-art baselines and even exceeds several supervised methods.展开更多
By means of polymerase chain reaction(PCR) technique,direct smear fluorescence microscopy and bacterial culture,the sputa and purulent secretion of 122 TB patients were examined to detect mycobacterium tuberculosis.
The thickness of two-dimensional(2D)nanomaterials shows a significant effect on their optical and electrical properties.Therefore,a rapid and automatic detection technology of 2D nanomaterials with desired layer-numbe...The thickness of two-dimensional(2D)nanomaterials shows a significant effect on their optical and electrical properties.Therefore,a rapid and automatic detection technology of 2D nanomaterials with desired layer-number is required to extend their practical application in optoelectronic devices.In this paper,an image recognition technology was proposed for rapid and reliable identification of thin-layer WS_(2) samples,which combining a layer-thickness identification criterion and a novel image segmentation algorithm.The criterion stemmed from optical contrast study of monochromatic illumination photographs,and the algorithm was based on Canny operator and edge connection iteration.This optical identification method can seek out thin-layer WS_(2) samples on complex surfaces,which provides a promising approach for automatic search of thin-layer nanomaterials.展开更多
基金supported by the National Natural Science Foundation of China(No.81030052,20907074)National Science & Technology Supporting Program(2012BAJ25B03-02)Tianjin Science & Technology Program(11ZCKFSF01100)
文摘Atrazine(AT,2-chloro-4-ethylamino-6-isopropyl-amino-s-triazine)has been detected in ground water in several areas of the United States for many years,as well as in China,wherein the growth rate of its gross
基金funded by the National Key Science and Technology Projects of China(2012ZX10004219 and 2013ZX10004001)
文摘A set of universal loop-mediated isothermal amplification (LAMP) primers targeting the flo gene was designed to detect Borrelia burgdorferi sensu lato (B. burgdorferi s.I.) in human samples. The sensitivity of LAMP was 20 copies/reaction, and the assay did not detect false positives among 11 other related bacteria. A positive LAMP result was obtained for 9 of the 24 confirmed cases and for 12 of 94 suspected cases. The positive rate of LAMP was the same as that of nested PCR. The LAMP is a useful diagnostic method that can be developed for rapid detection of B. burgdorferi s.I. in human sera. Combination of the LAMP and nested PCR was more sensitive for detecting B. burgdorferi s.I. in human serum samples.
基金financially supported by the Natural Science Foundation of Shanxi Province of China(201901D111210)Key Research Project of Science and Technology Plan in Jinzhong-Social Development Projects(Y213003)+1 种基金Special Project of Lvliang for Introduced High-level Science and Technology Talents(2021RC-2-1)Transverse Scientific Research Project of Shanxi Taiyuan Pharmaceutical Co.Ltd(2F022022006)
文摘Owing to the excellent stability,biocompatibility and photoluminescence property,graphene quantum dots(GQDs)are emerging as a kind of potential materials to be applied in a series of fields ranging from sensor to drug delivery.As the growing concern for human and environmental safety,selective detection of metal ions has been paid more and more attention.GQDs,as nanoparticles with superior optical properties,have been attracting growing attention in the field of metal ions detection.In this work,glutathione(GSH)functionalized boron doped graphene quantum dots(B-GQDs@GSH)were successfully synthesized with stable bright blue fluorescence and has been used for the detection of Fe^(3+).A good linear relationship between 1/(F_(0)-F)and 1/c with the concentration ranging from 0.70 to 53μmol/L was obtained with a detection limit of 5.5 nmol/L.The application of B-GQDs@GSH for Fe^(3+)detection in water samples was demonstrated and the quenching mechanism was further explored.Due to low cytotoxicity and favorable biocompatibility,B-GQDs@GSH were successfully applied for cell fluorescence imaging and intracellular determination of Fe^(3+).
基金supported by the National Key Research and Development Program of China(No.2021YFB3300503)Regional Innovation and Development Joint Fund of National Natural Science Foundation of China(No.U22A20167)National Natural Science Foundation of China(No.61872260).
文摘Recently,self-supervised learning has shown great potential in Graph Neural Networks (GNNs) through contrastive learning,which aims to learn discriminative features for each node without label information. The key to graph contrastive learning is data augmentation. The anchor node regards its augmented samples as positive samples,and the rest of the samples are regarded as negative samples,some of which may be positive samples. We call these mislabeled samples as “false negative” samples,which will seriously affect the final learning effect. Since such semantically similar samples are ubiquitous in the graph,the problem of false negative samples is very significant. To address this issue,the paper proposes a novel model,False negative sample Detection for Graph Contrastive Learning (FD4GCL),which uses attribute and structure-aware to detect false negative samples. Experimental results on seven datasets show that FD4GCL outperforms the state-of-the-art baselines and even exceeds several supervised methods.
文摘By means of polymerase chain reaction(PCR) technique,direct smear fluorescence microscopy and bacterial culture,the sputa and purulent secretion of 122 TB patients were examined to detect mycobacterium tuberculosis.
基金We acknowledge support from the National Natural Science Foundation of China(Nos.51527901,11890672,and 51705285).
文摘The thickness of two-dimensional(2D)nanomaterials shows a significant effect on their optical and electrical properties.Therefore,a rapid and automatic detection technology of 2D nanomaterials with desired layer-number is required to extend their practical application in optoelectronic devices.In this paper,an image recognition technology was proposed for rapid and reliable identification of thin-layer WS_(2) samples,which combining a layer-thickness identification criterion and a novel image segmentation algorithm.The criterion stemmed from optical contrast study of monochromatic illumination photographs,and the algorithm was based on Canny operator and edge connection iteration.This optical identification method can seek out thin-layer WS_(2) samples on complex surfaces,which provides a promising approach for automatic search of thin-layer nanomaterials.