Effect of alprostadil combined with Diammonium glycyrrhizinate on renal interstitial fibrosis in SD rats

Objective:To observe effect of alprostadil combined with Diammonium glycyrrhizinate on renal interstitial fibrosis in SD rate.Methods:A total of 75 SD rate were randomly divided into A,B,C,D,E groups with 15 in each group.Rats in group A served as the control group received just only but tissue separation without modeling operation,while model of unilateral ureteral obstruction(UUO) was established in B,C,D,E groups.Rats in A,B group were given saline lavage placebo treatment,while rats in C,D,E groups were given dianunonium glycyrrhizinate and alprostadil injection.Five rats were sacrificed 1,2,3 weeks after modeling,serum creatinine level of femoral venous blood was determined.Transforming growth factor- β1(TCF- β1) and concentration of connective tissue growth factor(CTGF) were also detected by using ELISA.Line renal interstitial tissue was taken after HE staining,renal interstitial TGF- β1 and CTGF expression were detected by using immunohistochemical method.Results:Serum creatinine levels of B,C,D,E group at different time points in were significantly higher than that of group A(P<0.05);serum creatinine levels in group B were significantly higher than that of C,D,E group at each time point(P<0.05).Serum creatinine level of Croup E was significantly lower than C,D group after 2,3 weeks(P<0.05).Rate in A group at each time point showed no significant changes in TGF- β1 and CREA concentration in serum and kidney tissues(P>0.05);while serum and kidney tissue TGF- β1,concentration of CREA.expression of rats in B,C,D,E groups showed a gradual increasing trend over time.TCF- β1 and CREF of Group B in serum and kidney tissues at each time point were significantly higher than that of the other groups(P<0.05).TCF- β1 and CREF of Group E in serum and kidney tissues at each time point were significantly lower than that of B,C,D group at all time points in serum and kidney tissues(P<0.05).Conclusions:Alprostadil combined with diammonium glycyrrhizinate can significantly lower the expression of TGF- β1 and CTGF in serum and tissues of SD rat with renal interstitial fibrosis,thus inhibit rat renal interstitial fibrosis process.It has synergy protective effect.

Anti-inflammatory effect of Diammonium Glycyrrhizinate in a rat model of ulcerative colitis

AIM： To explore the anti-inflammatory mechanism of Diammonium Glycyrrhizinate in a rat model of ulcerative colitis induced by acetic acid. METHODS： Spragur-Dawley female rats were divided into four groups： Diammonium Glycyrrhizinate group, dexamethasone group, acetic acid control and normal control group. Colonic inflammation was evaluated by disease activity index, gross morphologic damage, histological injury and colonic myeloperoxidase activity. Immunohistochemistry was used to detect the expression of NF-κB, TNF-α and ICAM-1 in colonic mucosa. RESULTS： Compared to the acetic acid control, both Diammonium Glycyrrhizinate and dexamethasone showed a significant anti-inflammatory effect （P 〈 0.01）. The expression of NF-κB, TNF-α and ICAM-1 in colonic mucosa was significantly lower in the Diammonium Glycyrrhizinate group and dexamethasone group than in the acetic acid group. CONCLUSION： Diammonium Glycyrrhizinate could reduce inflammatory injury in a rat model of ulcerative colitis. This may occur via suppression of NF-κB, TNF-αand ICAM-1 in colonic mucosa.

Effect of Fertilizer Diammonium Phosphate on Liver,Kidney and Muscle 5-Nucleotidase Activity of Fresh Water Teleost Fish Clarias batrachus

The toxic effect of fertilizer Diammonium phosphate resulted in alterations of 5'-Nucleotidase activity of tissues liver, kidney and muscles offish C. batrachus at varying intervals and exposures. Alterations in 5'-Nuclcotidase activity of body organs gave an idea of the toxicity caused by the fertilizer. Thus the enzyme 5'-Nucleotidase can be used to monitor the pollution in aquatic ecosystem.

Modulating CsPbl_(3)crystallization by using diammonium agent for efficient solar cells

Cesium lead triiodide(CsPbI_(3))perovskite receives tremendous attention for photovoltaic applications,owing to its remarkable thermal stability and optoelectronic properties.However,realizing the CsPbI_(3)perovskite with high black-phase stability and optoelectronic properties remains a significant challenge,which largely affects the photovoltaic performance of perovskite solar cells(PSCs).Herein,aromatic ammonium agents are used to modulate the crystallization of the CsPbI_(3)perovskite to improve its black-phase stability and optoelectronic properties for efficient PSCs.Systemically experimental studies and comprehensively theoretical calculations are performed,which reveal that histammonium dihydrochloride(HACl_(2))could strongly couple with the perovskite during its crystallization,leading to faster nucleation and slower perovskite growth,and thus modulating the crystallization dynamics of the perovskites.Moreover,the residual diammonium cations(HA^(2+))distributed at the grain boundaries and on the surface of the perovskites can effectively passivate defects through electrostatic interactions,substantially suppressing trap-assisted nonradiative recombination,and prompting more matched perovskite surface energetics.Consequently,the photovoltaic performance of CsPbI_(3)PSCs is largely improved because of a combination of enhanced crystallinity and optoelectronic properties of the perovskites.This work offers a new avenue to prepare inorganic perovskites with high optoelectronic properties for photovoltaics.

2种常用肥料在马铃薯种植条件下肥力转化速率研究

[目的]研究2种肥料在土壤中的转化时间,从而判断养分供给时间和能力,为确定科学施肥时间提供数据支持。[方法]以马铃薯生产中常用的2种肥料为处理,分别是硫酸钾、磷酸二铵。并设置3个土壤含水量处理,分别为25%、35%、45%。设定取样时间,测定肥料剩余量并计算肥料的溶解量和溶解率。[结果]磷酸二铵和硫酸钾分别在施肥后第5天和第56天时溶解量超过或接近50%,可将其标定最佳养分吸收期。磷酸二铵施入21 d后,肥料不再转化,整体上随着土壤水分含量的提高,磷酸二铵溶解能力增强,养分供应能力变大,但持续供养能力变弱。试验周期内硫酸钾养分释放比较平均,在土壤含水量25%和35%条件下,施肥后28 d内为养分高效供应期,28 d后养分供应趋于稳定。在土壤含水量45%条件下,施肥后49 d内为养分高效供应期。整体上看,含水量45%更有利于硫酸钾高效供养时间的延长。[结论]可将磷酸二铵和硫酸钾的溶解能力作为肥料养分供应的指标,但只能应用于矿质元素类肥料,因有机肥料溶解后还涉及矿质化过程,对有机类肥料不具备指导意义。

甘草酸二铵减轻肺结核模型大鼠肺损伤

目的探讨甘草酸二铵(DG)对肺结核模型大鼠肺损伤的影响。方法构建肺结核大鼠模型,将造模成功大鼠随机分为模型组(model组)、甘草酸二铵低、中、高剂量组(L-DG、M-DG、H-DG组)、甘草酸二铵高剂量+过氧化物酶体增殖物激活受体γ(PPARγ)抑制剂组(H-DG+GW9662组),另取18只作为对照组(control组);检测肺组织结核分枝杆菌(Mtb)菌落数;HE染色检测肺组织病理学变化;TUNEL检测肺组织细胞凋亡;ELISA检测血清炎性因子水平;Western blot检测肺组织PPARγ、磷酸化p38丝裂原活化蛋白激酶(p-p38MAPK)/p38丝裂原活化蛋白激酶(p38MAPK)表达。结果模型组较对照组肺组织结构破坏严重,产生大量增生型结核结节,肺泡形态发生变化,炎性细胞浸润明显,甚至出现干酪样坏死现象,结核菌菌落数增多,细胞凋亡率、TNF-α、IL-6、IFN-γ、COX-2水平及p-p38MAPK/p38MAPK表达升高,PPARγ表达降低(P<0.05)。L-DG、M-DG、H-DG组较模型组肺组织结构、肺泡形态、炎性细胞浸润、干酪样坏死等现象均有所改善,结核菌菌落数减少,细胞凋亡率降低,TNF-α、IL-6、IFN-γ、COX-2水平及p-p38MAPK/p38MAPK表达降低,PPARγ表达升高,其中H-DG组变化最显著(P<0.05)。GW9662处理可部分逆转DG对肺结核大鼠的肺损伤改善作用。结论DG可改善肺结核大鼠的肺损伤,其作用机制可能与激活PPARγ/p38MAPK通路有关。

护肝布祖热颗粒治疗药物性肝损伤的疗效观察

目的探讨护肝布祖热颗粒治疗药物性肝损伤的疗效及安全性。方法选取我院61例抗精神病药致药物性肝损伤患者,随机分为观察组(30例)和对照组(31例)。对照组在常规抗精神病用药治疗的基础上服用甘草酸二铵胶囊,观察组在常规抗精神病用药治疗基础上服用护肝布祖热颗粒,比较两组患者治疗6周后的临床疗效、肝功能及不良反应发生情况。结果观察组治疗6周后,肝功能谷丙转氨酶(ALT)、谷草转氨酶(AST)降低,指标优于对照组(P<0.05)。治疗后两组中医症候积分均有明显改善(P<0.05),且两组患者均未见明显不良反应。结论观察组在降低抗精神病药所致药物性肝损伤患者肝功能ALT、AST指标上优于对照组,起到较好的保肝护肝的作用,可有效帮助患者改善恶心呕吐、口干口苦等临床症状,且用药安全,值得临床推广应用。

甘草酸二铵通过TLR4/NF-κB信号通路负调控LPS诱导的BV2小胶质细胞神经炎症反应

目的:评价甘草酸二铵(DG)对脂多糖(LPS)诱导的BV2小胶质细胞TLR4/NF-κB炎症信号通路的影响,探讨其改善神经炎症的潜在机制。方法:采用LPS诱导BV2小胶质细胞损伤模型,给予20、60μmol/L DG处理。MTS检测细胞活力;Griess检测细胞NO分泌水平;ELISA检测细胞上清中TNF-α、IL-6和IL-1β炎症因子水平。RT-qPCR检测细胞TNF-α、IL-6、IL-1β、TLR4和MyD88 mRNA水平;Western blot检测细胞TLR4、MyD88、NF-κB、p-NF-κB、IκB-α和p-IκB-α蛋白表达。结果:与对照组相比,LPS处理的BV2小胶质细胞活力明显降低(P<0.05),NO分泌显著增加(P<0.05),TNF-α、IL-6和IL-1β炎症因子水平明显升高(P<0.05),TNF-α、IL-6、IL-1β、TLR4和MyD88 mRNA水平显著升高(P<0.05),TLR4、MyD88、p-NF-κB和p-IκB-α蛋白表达明显升高(P<0.05);与LPS组相比,不同浓度DG干预的BV2小胶质细胞活力明显升高(P<0.05),NO分泌明显减少(P<0.05),TNF-α、IL-6和IL-1β炎症因子水平明显降低(P<0.05),TNF-α、IL-6、IL-1β、TLR4和MyD88 mRNA水平明显降低(P<0.05),TLR4、MyD88、p-NF-κB和p-IκB-α蛋白表达明显降低(P<0.05)。结论:DG可有效减轻LPS诱导的小胶质细胞神经炎症反应,可能与抑制TLR4/NF-κB信号通路有关。

Preparation, Crystal Structure and Properties of a New Crystal Form of Diammonium 5,5＇-bistetrazole-1,1＇-diolate

A new crystal form of diammonium 5,5＇-bistetrazole-1,1＇-diolate （1） was prepared by two novel methods and fully characterized by using IR, NMR spectroscopy, elementary analysis, single crystal X-ray crystallography and thermal gravity/differential thermal analysis （TG/DTA）. Crystalline 1 was found as monoclinic and space group of P21/c （14）. The TG/DTA analysis showed that the decomposition temperature of 1 was 287.8℃ with a mass loss of 91.2% in the range of 220-300℃ at a heating rate of 5℃/min. The sensitivities test towards impact, friction of 1 indicated that 1 has much lower sensitivities than those of RDX/HMX and is comparable to those of TNT, which suggested that 1 could be used as a good candidate of new insensitive energetic compound.

多烯磷脂酰胆碱联合甘草酸二铵治疗老年脂肪肝患者的效果及对炎症因子的影响

目的研究多烯磷脂酰胆碱联合甘草酸二铵治疗老年脂肪肝患者的效果及对炎症因子的影响。方法回顾性分析82例老年脂肪肝患者的临床资料,根据用药方案不同分为对照组(41例)和观察组(41例)。对照组采用多烯磷脂酰胆碱治疗,观察组采用多烯磷脂酰胆碱联合甘草酸二铵治疗。比较两组临床疗效及治疗前后血脂功能指标[总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)]、炎症因子[血清中肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)]、肝功能指标[谷草转氨酶(AST)、谷丙转氨酶(ALT)]。结果两组治疗后TC、TG、LDL-C均较治疗前下降,HDL-C均较治疗前上升,且观察组TC(4.30±0.65)mmol/L、TG(1.38±0.25)mmol/L、LDL-C(2.13±0.38)mmol/L均较对照组的(5.09±0.78)、(1.74±0.31)、(2.61±0.46)mmol/L低,HDL-C(1.48±0.18)mmol/L较对照组的(1.24±0.11)mmol/L高(P<0.05)。两组治疗后TNF-α、IL-6均较治疗前下降,且观察组TNF-α(21.44±4.23)ng/L、IL-6(10.36±2.38)ng/L均低于对照组的(29.79±3.34)、(18.02±2.69)ng/L(P<0.05)。两组治疗后ALT、AST均较治疗前下降,且观察组ALT(42.56±6.51)U/L、AST(38.86±4.69)U/L均低于对照组的(63.91±7.89)、(58.02±5.92)U/L(P<0.05)。观察组总有效率95.12%高于对照组的80.49%(P<0.05)。结论多烯磷脂酰胆碱联合甘草酸二铵治疗老年脂肪肝的疗效显著,可协同改善脂代谢和肝功能,减轻炎症反应,利于受损肝组织修复,促进疾病转归。

甘草酸二铵胶囊治疗老年肝功能不全患者的效果

目的探究用甘草酸二铵胶囊治疗老年肝功能不全患者的疗效。方法将104例老年肝功能不全患者按照随机数字表法分为对照组和观察组,每组52例。对照组给予常规治疗,观察组在对照组治疗的基础上口服甘草酸二铵胶囊。比较2组患者的临床疗效和不良反应发生率,比较2组患者治疗前后的肝功能[丙氨酸氨基转移酶(alanine aminotransferase,ALT)、天冬氨酸氨基转移酶(aspartate aminotransferase,AST)、总胆红素(total bilirubin,TBiL)]、炎症因子[C反应蛋白(C-reactive protein,CRP)、中性粒细胞计数(neutrophil count,NEUT)、白细胞计数(white blood cell count,WBC)]、应激反应[丙二醛(malondialdehyde,MDA)、超氧化物歧化酶(super oxide dismutase,SOD)、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)]的水平。结果观察组的治疗总有效率为96.15%,高于对照组的82.69%(P<0.05);治疗后,观察组的ALT、AST、TBiL、CRP、WBC和MDA水平均低于对照组,SOD及GSH-Px水平均高于对照组(P<0.05)。结论在常规保肝治疗的基础上联用甘草酸二铵胶囊治疗老年肝功能不全患者,可促进患者肝功能的恢复,抑制炎症与应激反应,有较好的保肝效果与安全性。

不同磷酸二铵对冬小麦产量·经济效益和土壤特性的影响

为研究不同磷酸二铵对冬小麦产量、经济效益及土壤特性的影响,以“济麦22”为供试小麦品种,选用5种不同种类的磷酸二铵进行施肥处理试验,试验周期为2年,磷酸二铵含P 2O 5占比为39%(T_(1)、T_(2)),46%(T_(3)、T_(4)),40%(T_(5)),空白对照不施肥(T_(0)),所有处理尿素(含N 45%)、硫酸钾(含K 2O 50%)分别等量施用。结果表明,小麦分蘖期分别为T_(2)、T_(1)地上干重最大;返青拔节期地上干重、分蘖数分别为T_(2)、T_(1)最大,全氮、全磷、全钾除T_(0)外,各处理间差异不显著;挑旗期地上干重均为T_(2)最大,全氮、全磷、全钾除T_(0)外,各处理间差异不显著;灌浆期地上干重分别为T_(2)、T_(3)最大,全氮、全磷、全钾除T_(0)外,各处理间差异不显著;收获期株高,秸秆和籽粒的全氮、全磷、全钾除T_(0)外,各处理间差异不显著;籽粒产量、经济系数、相对净收入、产投比T_(1)、T_(2)最大;籽粒吸磷量、秸秆吸磷量、地上部吸磷量、磷肥效、磷肥农学利用率为T_(1)、T_(2)最高;随着施磷时间增加,土壤中全氮含量不受影响,有效磷含量降低,速效钾含量不受影响,pH降低,表明磷肥会降低土壤pH。综上所述,P 2O 5占比为39%的磷酸二铵在德州地区冬小麦种植中表现较好。

60万t/a磷酸二铵装置气体系统操作控制的分析与优化

针对云南天安化工有限公司二期60万t/a磷酸二铵装置生产过程流化冷却含尘气体直接排放污染环境、产品温度达不到控制指标、烟囱排放颗粒物高及洗涤系统洗液中和度居高不下等问题,提出了操作控制的改进措施,并取得了显著的优化效果。

双环醇联合甘草酸二铵对乙肝患者肝功能及肝纤维化程度的影响

目的探讨双环醇联合甘草酸二铵对乙肝患者肝功能及肝纤维化程度的影响。方法使用随机数字表法将2020年9月至2023年9月赣州市南康区第一人民医院收治的82例乙肝患者分为对照组与观察组。对照组(41例)予以甘草酸二铵,观察组(41例)予以双环醇联合甘草酸二铵。比较两组的肝功能及肝纤维化水平。结果与治疗前相比,两组治疗6周后的谷草转氨酶(AST)、谷丙转氨酶(ALT)和总胆红素(TBIL)的水平均明显下降(P<0.05)。观察组治疗后的粘连蛋白(LN)、透明质酸(HA)和Ⅲ型前胶原(HPCⅢ)均较治疗前降低,且低于对照组(P<0.05)。观察组肝硬度值(LSM)低于对照组(P<0.05)。观察组肝纤维化程度较对照组更低(P<0.05)。结论对乙肝患者给予双环醇联合甘草酸二铵的保肝治疗,临床疗效确切,可改善患者肝功能,减轻肝纤维化程度。

60万t/a磷酸二铵装置水冷却系统技术改造

云南某公司二期的60万t/a磷酸二铵(DAP)冷却系统,设计使用风冷流化床,存在产品包裹温度高、包裹后产品外观质量波动较大、风冷系统尾气排放量大、后续尾气治理工艺路线选择及治理难度大等问题。针对问题,对产品冷却系统进行了有针对性的技术改造。采用水冷却器,使DAP产品的包裹温度得到了有效控制,使装置的尾气排放量降低,也使装置的能耗下降。通过装置的性能考核,验证了水冷却器技术的应用效果。

Effects of diammonium glycyrrhizinate on hepatic and intestinal UDP-Glucuronosyltransferases in rats: Implication in herb-drug interactions

Glycyrrhizin is a major bioactive component of liquorice, which exerts multiple biochemical and pharmacological activities and is frequently used in combination with other drugs in the clinic. Mycophenolate mofetil(MMF), an immunosuppressant widely used in transplant patients, is metabolized by UDP-glucuronyltransferases(UGTs). Although significant evidence supports that glycyrrhizin could interact with the cytochrome P450s(CYPs), few studies have addressed its effects on UGTs. The present study aimed at investigating the regulatory effects of diammonium glycyrrhizinate(GLN) on UGTs in vitro and in vivo. We found that long-term administration of GLN in rats induced overall metabolism of MMF, which might be due to the induction of UGT1A protein expression. Hepatic UGT1A activity and UGT1A mRNA and protein expression were significantly increased in GLN-treated rats. UGT1A expression levels were also increased in the intestine, contradicting with the observed decrease in intestinal UGT1A activities. This phenomenon may be attributed to different concentrations of glycyrrhetinic acid(GA) in liver and intestine and the inhibitory effects of GA on UGT1A activity. In conclusion, our study revealed that GLN had multiple effects on the expression and activities of UGT1A isoforms, providing a basis for a better understanding of interactions between GLN and other drugs.

恩替卡韦联合苦黄注射液及甘草酸二铵注射液治疗慢性肝炎的价值研究

目的分析恩替卡韦+苦黄注射液+甘草酸二铵注射液方案对慢性肝炎治疗的价值。方法方便选取2021年11月—2022年11月在滨海县人民医院接受治疗的97例慢性肝炎患者为研究对象,利用随机数表法分为两组。对照组(n=48)给予西药恩替卡韦治疗,观察组(n=49)在对照组的基础上加用苦黄注射液和甘草酸二胺注射液。对比两组患者肝功能、肝纤维化指标和临床治疗效果。结果治疗后,观察组总胆红素、门冬氨酸氨基转氨酶、丙氨酸氨基转氨酶水平均低于对照组,差异有统计学意义(P均<0.05)。观察组治疗总有效率为93.88%,高于对照组的79.17%,差异有统计学意义(χ^(2)=4.521,P=0.033)。观察组肝纤维化指标均优于对照组,差异有统计学意义(P均<0.05)。结论恩替卡韦联合苦黄注射液及甘草酸二铵注射液治疗慢性肝炎效果显著,可改善患者肝脏功能。

分光光度法在含聚合态磷磷酸二铵生产中的应用

离子色谱法或离子交换树脂法测定含聚合态磷磷酸二铵聚合率,存在检测设备昂贵或检测耗时较长的问题。采用国家标准《磷酸一铵、磷酸二铵的测定方法第2部分:磷含量》(GB/T 10209.2—2010)中的重量法测定含聚合态磷磷酸二铵中有效磷的含量,分光光度法测定正磷酸盐含量,然后计算聚合率,可在3.5 h左右获得测定结果。实验室测试及实际生产应用结果表明,采用分光光度法测定含聚合态磷磷酸二铵的聚合率,可以满足生产控制要求。

甘草酸二铵对颅脑损伤大鼠神经元活性、脑组织病理及YAP1蛋白的影响

目的探讨甘草酸二铵(DG)对颅脑损伤大鼠神经元活性、脑组织病理及YES关联蛋白1(YAP1)蛋白的研究。方法将44只SD大鼠随机分为4组,每组11只,健康组、外伤组(颅脑外伤模型)、依达组(颅脑外伤模型给予依达拉奉干预)、甘草组(颅脑外伤模型给予DG干预)。采用Zea Longa法(5分)检测各组大鼠神经功能;用酶联免疫法测定血清白介素-6(IL-6)、IL-1β和肿瘤坏死因子-α(TNF-α)水平;采用HE染色观察脑组织神经细胞形态学变化;TUNEL法检测各组大鼠海马组织神经细胞凋亡;免疫印迹检测海马组织YAP1、磷酸化YAP1(p-YAP1)、大肿瘤抑制基因1(Lats1)及磷酸化Lats1(p-Lats1)蛋白表达水平。结果与健康组相比,外伤组神经功能评分,IL-6、TNF-α、IL-1β,神经细胞凋亡、YAP1、p-YAP1、Lats1、p-Lats1均升高(P<0.05);与外伤组相比,依达组及甘草组以上指标均降低(P<0.05),依达组和甘草组比较,以上指标差异无统计学意义(P>0.05);与健康组相比,外伤组大鼠海马组织结构紊乱,神经元数目减少,细胞核深染,细胞膜部分消失,依达组和甘草组神经元排列比外伤组整齐有序,肿胀程度减轻,细胞核较清晰。结论甘草酸二铵对颅脑损伤的治疗具有一定积极作用,且与依达拉奉治疗相关相当,其机制可能与改善神经功能、调节炎症因子变化及相关蛋白表达相关。

探讨益肝灵联合甘草酸二铵治疗抗结核药物性肝损伤的疗效

目的 探讨益肝灵联合甘草酸二铵治疗抗结核药物性肝损伤(ATB-DILI)的疗效,并分析其对肝功能、炎性因子水平、免疫功能的影响。方法 选取2021年6月至2023年6月鹿邑县疾病预防控制中心收治的80例ATB-DILI患者为研究对象,随机分为单一组40例、联合组40例。单一组予以甘草酸二铵治疗,联合组予以益肝灵联合甘草酸二铵治疗。统计对比两组临床疗效、不良反应、临床症状积分及治疗前后肝功能[丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)、总胆红素(TBIL)、γ-谷氨酰转肽酶(γ-GT)]、炎性因子[白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)]、外周血T淋巴细胞亚群(CD3^(+)、CD4^(+)、CD8^(+))水平变化。结果 联合组治疗有效率高于单一组,差异有统计学意义(P <0.05);治疗后,联合组临床症状积分低于单一组,差异有统计学意义(P <0.05);治疗后,联合组血清ALT、AST、TBIL、γ-GT、IL-6、TNF-α、IL-1β水平低于单一组,差异均有统计学意义(P <0.05);治疗后,联合组CD3^(+)、CD4^(+)高于单一组,CD8^(+)低于单一组,差异均有统计学意义(P <0.05);联合组不良反应发生率与单一组比较差异无统计学意义(P> 0.05)。结论 益肝灵联合甘草酸二铵治疗ATB-DILI的疗效确切且具有一定安全性,可改善临床症状,促进免疫功能、肝功能恢复,抑制炎性反应。