A severe leaf spot disease on patchouli,was observed in Guangdong,China.Early symptom appeared as circular or irregularly shaped and dark brown spots along the leaf margins and tips.As the disease progressed,the spots...A severe leaf spot disease on patchouli,was observed in Guangdong,China.Early symptom appeared as circular or irregularly shaped and dark brown spots along the leaf margins and tips.As the disease progressed,the spots enlarged and with masses black,sessile conidiomata were observed in lesions.The results of morphology,molecular biology and pathogenicity test indicate that the causal agent of leaf disease of patchouli is Diaporthe arecae.展开更多
Three new azaphilone compounds,isochromophilones X-XII(1-3),together with two known ones sclerotioramine(4)and isochromophilone VI(5)were isolated from the cultures of an endophytic fungus Diaporthe sp.The structures ...Three new azaphilone compounds,isochromophilones X-XII(1-3),together with two known ones sclerotioramine(4)and isochromophilone VI(5)were isolated from the cultures of an endophytic fungus Diaporthe sp.The structures were elucidated by extensive HRESIMS and NMR spectroscopic analyses.All compounds were tested for their cytotoxicities against five human cancer cell lines by MTT method,among which compound 1 showed moderate inhibitory effects on these cell lines.This was the first report of azaphilones isolated from Diaporthe sp.展开更多
t Six new species of Diaporthe,D.beilharziae on Indigofera australis,D.fraxini-angustifoliae on Fraxinus angustifolia subsp.oxycarpa,D.litchicola on Litchi chinensis,D.nothofagi on Nothofagus cunninghamii,D.pascoei on...t Six new species of Diaporthe,D.beilharziae on Indigofera australis,D.fraxini-angustifoliae on Fraxinus angustifolia subsp.oxycarpa,D.litchicola on Litchi chinensis,D.nothofagi on Nothofagus cunninghamii,D.pascoei on Persea americana and D.salicicola on Salix purpurea from Australia are described and illustrated based on morphological characteristics and molecular analyses.Three of the new species no longer produced sporulating structures in culture and two of these were morphologically described from voucher specimens.Phylogenetic relationships of the new species with other Diaporthe species are revealed by DNA sequence analyses based on the internal transcribed spacer(ITS)region,and partial regions of theβtubulin(BT)and translation elongation factor 1-alpha(TEF).展开更多
Diaporthe species are important pathogens of a wide range of hosts including many economically important crops.Diaporthe citri is generally accepted to cause melanose of Citrus fruits,leaves and shoots;stem-end rot of...Diaporthe species are important pathogens of a wide range of hosts including many economically important crops.Diaporthe citri is generally accepted to cause melanose of Citrus fruits,leaves and shoots;stem-end rot of fruits and gummosis of perennial branches.In this study,147 strains of Diaporthe were isolated from diseased leaves,twigs,fruits and branches of Citrus from Shaanxi,Zhejiang,Guangxi,Fujian,Guangdong,Jiangxi,Hunan,Chongqing and Shanghai and identified by morphology and molecular analysis of nuclear ribosomal internal transcribed spacer(ITS),partial sequences of translation elongation factor 1-α(EF 1-α),tubulin(TUB)and calmodulin(CAL)genes.All strains from Citrus clustered in three clades.Diaporthe citri was the predominant species and is compared with strains from Florida,USA,where this species was first collected and described.Two new taxa differing from D.citri are described as D.citriasiana and D.citrichinensis in this paper.Inoculation tests indicate that all three species are able to cause stem-end rot of fruits,while only D.citri could cause melanose on leaves.展开更多
基金Supported by Science and Technology Project of Guangdong Province in China(2017A020213003)the Opening Project Fund of Key Laboratory of Guangdong Institute of Traditional Chinese Medicine(GDTCMZJ201901)。
文摘A severe leaf spot disease on patchouli,was observed in Guangdong,China.Early symptom appeared as circular or irregularly shaped and dark brown spots along the leaf margins and tips.As the disease progressed,the spots enlarged and with masses black,sessile conidiomata were observed in lesions.The results of morphology,molecular biology and pathogenicity test indicate that the causal agent of leaf disease of patchouli is Diaporthe arecae.
基金This work was co-financed by National Natural Science Foundation of China(81121062,21132004,90813036,and 81172948),MOE(NCET-10-0477)Jiangsu Provincial Government(BK2009010).
文摘Three new azaphilone compounds,isochromophilones X-XII(1-3),together with two known ones sclerotioramine(4)and isochromophilone VI(5)were isolated from the cultures of an endophytic fungus Diaporthe sp.The structures were elucidated by extensive HRESIMS and NMR spectroscopic analyses.All compounds were tested for their cytotoxicities against five human cancer cell lines by MTT method,among which compound 1 showed moderate inhibitory effects on these cell lines.This was the first report of azaphilones isolated from Diaporthe sp.
文摘t Six new species of Diaporthe,D.beilharziae on Indigofera australis,D.fraxini-angustifoliae on Fraxinus angustifolia subsp.oxycarpa,D.litchicola on Litchi chinensis,D.nothofagi on Nothofagus cunninghamii,D.pascoei on Persea americana and D.salicicola on Salix purpurea from Australia are described and illustrated based on morphological characteristics and molecular analyses.Three of the new species no longer produced sporulating structures in culture and two of these were morphologically described from voucher specimens.Phylogenetic relationships of the new species with other Diaporthe species are revealed by DNA sequence analyses based on the internal transcribed spacer(ITS)region,and partial regions of theβtubulin(BT)and translation elongation factor 1-alpha(TEF).
基金supported by China Agriculture Research System(CARS-27)Research Programs for Nonprofit Technology and Application of Zhejiang Province,China(2009CB119203)。
文摘Diaporthe species are important pathogens of a wide range of hosts including many economically important crops.Diaporthe citri is generally accepted to cause melanose of Citrus fruits,leaves and shoots;stem-end rot of fruits and gummosis of perennial branches.In this study,147 strains of Diaporthe were isolated from diseased leaves,twigs,fruits and branches of Citrus from Shaanxi,Zhejiang,Guangxi,Fujian,Guangdong,Jiangxi,Hunan,Chongqing and Shanghai and identified by morphology and molecular analysis of nuclear ribosomal internal transcribed spacer(ITS),partial sequences of translation elongation factor 1-α(EF 1-α),tubulin(TUB)and calmodulin(CAL)genes.All strains from Citrus clustered in three clades.Diaporthe citri was the predominant species and is compared with strains from Florida,USA,where this species was first collected and described.Two new taxa differing from D.citri are described as D.citriasiana and D.citrichinensis in this paper.Inoculation tests indicate that all three species are able to cause stem-end rot of fruits,while only D.citri could cause melanose on leaves.
