<i>Fasciola hepatica</i>and Associated Parasite, <i>Dicrocoelium dendriticum</i>in Slaughter Houses in Anyigba, Kogi State, Nigeria

Fasciola hepatica is a parasite of clinical and veterinary importance which causes fascioliasis that leads to reduction in milk and meat production. Bile samples were centrifuged at 1500 rpm for ten (10) minutes in a centrifuge machine and viewed microscopically to check for F. hepatica eggs. A total of 300 bile samples of cattle which included 155 males and 145 females were collected from the abattoir. Results were analyzed using chi-square (p > 0.05). The prevalence of F. gigantica and Dicrocoelium dentriticum is 33.0% (99) and 39.0% (117) respectively. Age prevalence of F. hepatica revealed that 0 - 2 years (33.7%, 29 cattle) were more infected than 2 - 4 years (32.7%, 70 cattle) while for D. dentriticum age 2 - 4 years were more infected than 0 - 2 years with prevalence of 40.2% (86) and 36.0% (31) respectively. No significant difference (P > 0.05) existed in prevalence in ages of the cattle. Out of the 300 bile samples examined, 22.3% (67 cattle) were co-infected with F. hepatica and D. dendriticum. Males were more co-infected than females having a prevalence of 24.5% (38 cattle) and 20.0% (29 cattle) respectively. Based on the age, samples of age 0 - 2 years were more co-infected than those of age 2 - 4 years with a prevalence of 23.3% (20 cattle) and 22.0% (47 cattle) respectively. The findings of this present study revealed that efforts to alleviate problems of animal health and productivity are yet to make any significant impact as this poses threat on human health. Investigation on the pattern of infections in cattle slaughtered should be strengthened.

我国牛羊双腔类吸虫的继续研究:Ⅲ.三种双腔吸虫后蚴在异常昆虫宿主蚂蚁体内的发育

内蒙科尔沁草原的中华双腔吸虫(Dicrocoelium chinensis Tang et Tang,1978)其第二中间宿主是黑玉蚂蚁(Formica gagates),而山东滨州地区的矛形双腔吸虫(D.lanceatum Rud.,1803)和枝双腔吸虫(D.dendriticum Rud.,1819)的蚂蚁宿主是中华蚂蚁(Formica sinica)。作者用中华双腔吸虫的黏球感染山东的中华蚂蚁,同时用矛形双腔和枝双腔的黏球分别感染内蒙的黑玉蚂蚁。本文报道此试验中不同蚂蚁种类和各虫种后蚴间所产生的不同反应情况,及矛形双腔和枝双腔在后蚴各发育期的形态差异。

矛形双腔吸虫及枝双腔吸虫尾蚴粘液腺的组织化学观察

本文报道应用组织化学方法观察了矛形双腔吸虫［Ｄｉｃｒｏｃｏｅｌｉｕｍｌａｎｃｅａｔｕｍ（Ｒｕｄｏｌｐｈｉ，１８０３）］及枝双腔吸虫［Ｄｉｃｒｏｃｏｅｌｉｕｍｄｅｎｄｒｉｔｉｃｕｍ（Ｒｕｄｏｌｐｈｉ，１８１９）］尾蚴粘液腺的结构及其组织化学成分。结果表明，两种双腔吸虫尾蚴粘液腺细胞内含有粘蛋白、碱性蛋白质及多糖物质，但枝双腔吸虫的含量分别比矛形双腔吸虫更丰富。而且矛形双腔吸虫尾蚴粘液腺物质由微细稀疏的颗粒组成，如同网状分布；枝双腔吸虫尾蚴粘液腺物质由粗大密集的颗粒组成。

枝双腔吸虫的形态学鉴定及分子进化研究

双腔吸虫病为一种人兽共患性寄生虫疾病,据报道有3种双腔吸虫可以感染羊。为了解齐齐哈尔地区绵羊感染双腔吸虫的种类,本研究对齐齐哈尔地区绵羊胆管分离的吸虫根据虫体形态学初步鉴定为枝双腔吸虫;PCR扩增吸虫的r DNA ITS序列,测序后经NCBI数据库比对分析,结果显示本研究分离的吸虫与枝双腔吸虫同源性达到99.8%;基于ITS-2序列构建系统进化树,分析结果显示本研究分离的吸虫与不同宿主来源的枝双腔吸虫聚集在同一个分支,因此鉴定本研究分离的吸虫为枝双腔吸虫。本研究对齐齐哈尔地区绵羊感染双腔吸虫种类有了初步了解,并为双腔吸虫的流行病学调查和分子鉴别诊断提供科学的参考依据。

绵羊源矛形双腔吸虫形态学观察和PCR鉴定

为鉴定新疆和静县绵羊感染的双腔吸虫种类,分析其遗传进化特征,采用形态学观察和PCR法相结合的方法,对和静县绵羊肝胆管内分离出来的双腔吸虫进行了种类鉴定和序列分析。结果显示,经形态学观察,初步鉴定绵羊感染的吸虫为矛形双腔吸虫;基于吸虫的ITS序列,采用PCR法扩增该虫的DNA样本,测序所得序列与山西省周至县山羊源矛形双腔吸虫序列的同源性为100%,鉴定为矛形双腔吸虫。