A total of 44 HSV isolates from the patients was detected and typed by using HSV typespecific DNA probes labeled with Digoxigenin and 32 P respectively. 25 isolates were identified to be HSV-1, 19 were HSV-2. Compar...A total of 44 HSV isolates from the patients was detected and typed by using HSV typespecific DNA probes labeled with Digoxigenin and 32 P respectively. 25 isolates were identified to be HSV-1, 19 were HSV-2. Comparison of the typing results showed that the sensitivity and specificity of the Digoxigenin-labeled probe were equal to those of the 32 P-labeled Probe, and the typing rate of HSV isolates was 100%. The Dig-labeled probes are nonradioactive and the labeling technigue is simple, the Dig-labeled probes can be stored and reused, so they are more valuable for clinical application.展开更多
A new rapid technique for intrahepatic and extrahepatic HBV DNA detection by using digoxigenin labelled probe with in situ hybridization was developed.This technique has the advantage of being non-radioactive and a qu...A new rapid technique for intrahepatic and extrahepatic HBV DNA detection by using digoxigenin labelled probe with in situ hybridization was developed.This technique has the advantage of being non-radioactive and a quick procedure yielding stable results and showing a clear background.展开更多
文摘A total of 44 HSV isolates from the patients was detected and typed by using HSV typespecific DNA probes labeled with Digoxigenin and 32 P respectively. 25 isolates were identified to be HSV-1, 19 were HSV-2. Comparison of the typing results showed that the sensitivity and specificity of the Digoxigenin-labeled probe were equal to those of the 32 P-labeled Probe, and the typing rate of HSV isolates was 100%. The Dig-labeled probes are nonradioactive and the labeling technigue is simple, the Dig-labeled probes can be stored and reused, so they are more valuable for clinical application.
文摘A new rapid technique for intrahepatic and extrahepatic HBV DNA detection by using digoxigenin labelled probe with in situ hybridization was developed.This technique has the advantage of being non-radioactive and a quick procedure yielding stable results and showing a clear background.
