[Objectives] This study was conducted to determine capsaicin and dihydrocapsaicin in capsicum products. [Methods]The sample was ultrasonically extracted with anhydrous ethanol as the extraction solvent for capsaicin a...[Objectives] This study was conducted to determine capsaicin and dihydrocapsaicin in capsicum products. [Methods]The sample was ultrasonically extracted with anhydrous ethanol as the extraction solvent for capsaicin and dihydrocapsaicin,followed by centrifugation. The extract was subjected to HPLC separation with methanol-water( 65∶ 35) as the mobile phase,and a fluorescence detector( Ex = 229 nm,Em = 320 nm) was used to detect capsaicinoids in the sample. [Results] Capsaicin and dihydrocapsaicin had a good linear relationship in the range of 1-200 mg/L( R_1~2= 0. 999 8,R22= 0. 999 6). The detection limits were both 0. 007 mg/kg; the quantification limits were both 0. 02 mg/kg; the precision was 0. 235% and 0. 754%,respectively; and the average recoveries were95. 94% and 95. 39%,respectively. [Conclusions]The method is simple,rapid,with good sensitivity and precision,and is suitable for detecting capsaicin substances in capsicum products.展开更多
文摘[Objectives] This study was conducted to determine capsaicin and dihydrocapsaicin in capsicum products. [Methods]The sample was ultrasonically extracted with anhydrous ethanol as the extraction solvent for capsaicin and dihydrocapsaicin,followed by centrifugation. The extract was subjected to HPLC separation with methanol-water( 65∶ 35) as the mobile phase,and a fluorescence detector( Ex = 229 nm,Em = 320 nm) was used to detect capsaicinoids in the sample. [Results] Capsaicin and dihydrocapsaicin had a good linear relationship in the range of 1-200 mg/L( R_1~2= 0. 999 8,R22= 0. 999 6). The detection limits were both 0. 007 mg/kg; the quantification limits were both 0. 02 mg/kg; the precision was 0. 235% and 0. 754%,respectively; and the average recoveries were95. 94% and 95. 39%,respectively. [Conclusions]The method is simple,rapid,with good sensitivity and precision,and is suitable for detecting capsaicin substances in capsicum products.