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Adaptive Object Tracking Discriminate Model for Multi-Camera Panorama Surveillance in Airport Apron
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作者 Dequan Guo Qingshuai Yang +3 位作者 Yu-Dong Zhang Gexiang Zhang Ming Zhu Jianying Yuan 《Computer Modeling in Engineering & Sciences》 SCIE EI 2021年第10期191-205,共15页
Autonomous intelligence plays a significant role in aviation security.Since most aviation accidents occur in the take-off and landing stage,accurate tracking of moving object in airport apron will be a vital approach ... Autonomous intelligence plays a significant role in aviation security.Since most aviation accidents occur in the take-off and landing stage,accurate tracking of moving object in airport apron will be a vital approach to ensure the operation of the aircraft safely.In this study,an adaptive object tracking method based on a discriminant is proposed in multi-camera panorama surveillance of large-scale airport apron.Firstly,based on channels of color histogram,the pre-estimated object probability map is employed to reduce searching computation,and the optimization of the disturbance suppression options can make good resistance to similar areas around the object.Then the object score of probability map is obtained by the sliding window,and the candidate window with the highest probability map score is selected as the new object center.Thirdly,according to the new object location,the probability map is updated,the scale estimation function is adjusted to the size of real object.From qualitative and quantitative analysis,the comparison experiments are verified in representative video sequences,and our approach outperforms typical methods,such as distraction-aware online tracking,mean shift,variance ratio,and adaptive colour attributes. 展开更多
关键词 Autonomous intelligence discriminate model probability map scale adaptive tracking
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Multispectral Imaging in Combination with Multivariate Analysis Discriminates Selenite Induced Cataractous Lenses from Healthy Lenses of Sprague-Dawley Rats
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作者 Peter Osei-Wusu Adueming Moses Jojo Eghan +5 位作者 Benjamin Anderson Samuel Kyei Jerry Opoku-Ansah Charles L. Y. Amuah Samuel Sonko Sackey Paul Kingsley Buah-Bassuah 《Open Journal of Biophysics》 2017年第3期145-156,共12页
Cataracts are the leading cause of blindness worldwide. Current methods for discriminating cataractous lenses from healthy lenses of Sprague-Dawley rats during preclinical studies are based on either histopathological... Cataracts are the leading cause of blindness worldwide. Current methods for discriminating cataractous lenses from healthy lenses of Sprague-Dawley rats during preclinical studies are based on either histopathological or clinical assessments which are weakened by subjectivity. In this work, both cataractous and healthy lens tissues of Sprague-Dawley rats were studied using multispectral imaging technique in combination with multivariate analysis. Multispectral images were captured in transmission, reflection and scattering modes. In all, five spectral bands were found to be markers for discriminating cataractous lenses from healthy lenses;470 nm and 625 nm discriminated in reflection mode whereas 435 nm, 590 nm and 700 nm discriminated in transmission mode. With Fisher’s Linear discriminant analysis, the midpoints for classifying cataractous from healthy lenses were found to be 14.718 × 10&minus;14 and 3.2374 × 10&minus;14 for the two spectra bands in the reflection mode and the three spectral bands in the transmission mode respectively. Images in scattering mode did not show significant discrimination. These spectral bands in reflection and transmission modes may offer potential diagnostic markers for discriminating cataractous lenses from healthy lenses thereby promising multispectral imaging applications for characterizing cataractous and healthy lenses. 展开更多
关键词 MULTISPECTRAL Imaging Cataractous Lenses Principal Component ANALYSIS Fisher’s Linear DISCRIMINANT ANALYSIS
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Using Big Data to Discriminate Charged Price in the Car Insurance Industry: Evidence from United States
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作者 Doaa Salman Merna Nagy 《Proceedings of Business and Economic Studies》 2019年第6期1-6,共6页
The purpose of this paper is to examinethe gender-based discrimination in car insurance ratesand whether the reasons provided by the car insurancecompanies for the different rates are valid or not. Thepaper studies th... The purpose of this paper is to examinethe gender-based discrimination in car insurance ratesand whether the reasons provided by the car insurancecompanies for the different rates are valid or not. Thepaper studies the average annual premiums paid bymen and women across different age groups from 16years old to over 56 years old along with the percentagedifferences. Additionally, the concept of big data andhow it is utilized by businesses to apply personalizeprice discrimination is investigated. The researchdesign is conclusive and secondary data is used in bothqualitative and quantitative forms. Qualitative data iscollected from articles for the literature review andas for the quantitative data it is in the form of reportsand surveys. The data shows that at lower age groupswomen pay less than men for car insurance but as theage increase men start paying less. The paper reaches aconclusion that gender is not necessarily a crucial riskfactoras the regular factors such as driving record canprovided accurate risk determinants. 展开更多
关键词 Big Data PRICE DISCRIMINATION INSURANCE COMPANY
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Why Female College Graduates Are Discriminated in China?
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作者 王瑞祎 《神州》 2014年第15期266-266,共1页
The discrimination against female college graduates is a problem which cannot be ignored in Chinese labor market. This paper thus focuses on the analyses of why the discrimination exists in order to seek better soluti... The discrimination against female college graduates is a problem which cannot be ignored in Chinese labor market. This paper thus focuses on the analyses of why the discrimination exists in order to seek better solutions to the problem. 展开更多
关键词 DISCRIMINATION college FEMALE decided eventually GIVING UNTIL EMPLOYMENT themselves SPEAKING
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Underwater Pulse Waveform Recognition Based on Hash Aggregate Discriminant Network
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作者 WANG Fangchen ZHONG Guoqiang WANG Liang 《Journal of Ocean University of China》 SCIE CAS CSCD 2024年第3期654-660,共7页
Underwater pulse waveform recognition is an important method for underwater object detection.Most existing works focus on the application of traditional pattern recognition methods,which ignore the time-and space-vary... Underwater pulse waveform recognition is an important method for underwater object detection.Most existing works focus on the application of traditional pattern recognition methods,which ignore the time-and space-varying characteristics in sound propagation channels and cannot easily extract valuable waveform features.Sound propagation channels in seawater are time-and space-varying convolutional channels.In the extraction of the waveform features of underwater acoustic signals,the effect of high-accuracy underwater acoustic signal recognition is identified by eliminating the influence of time-and space-varying convolutional channels to the greatest extent possible.We propose a hash aggregate discriminative network(HADN),which combines hash learning and deep learning to minimize the time-and space-varying effects on convolutional channels and adaptively learns effective underwater waveform features to achieve high-accuracy underwater pulse waveform recognition.In the extraction of the hash features of acoustic signals,a discrete constraint between clusters within a hash feature class is introduced.This constraint can ensure that the influence of convolutional channels on hash features is minimized.In addition,we design a new loss function called aggregate discriminative loss(AD-loss).The use of AD-loss and softmax-loss can increase the discriminativeness of the learned hash features.Experimental results show that on pool and ocean datasets,which were collected in pools and oceans,respectively,by using acoustic collectors,the proposed HADN performs better than other comparative models in terms of accuracy and mAP. 展开更多
关键词 convolutional channel hash aggregate discriminative network aggregate discriminant loss waveform recognition
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LociScan,a tool for screening genetic marker combinations for plant variety discrimination
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作者 Yang Yang Hongli Tian +5 位作者 Hongmei Yi Zi Shi Lu Wang Yaming Fan Fengge Wang Jiuran Zhao 《The Crop Journal》 SCIE CSCD 2024年第2期583-593,共11页
To reduce the cost and increase the efficiency of plant genetic marker fingerprinting for variety discrimination,it is desirable to identify the optimal marker combinations.We describe a marker combination screening m... To reduce the cost and increase the efficiency of plant genetic marker fingerprinting for variety discrimination,it is desirable to identify the optimal marker combinations.We describe a marker combination screening model based on the genetic algorithm(GA)and implemented in a software tool,Loci Scan.Ratio-based variety discrimination power provided the largest optimization space among multiple fitness functions.Among GA parameters,an increase in population size and generation number enlarged optimization depth but also calculation workload.Exhaustive algorithm afforded the same optimization depth as GA but vastly increased calculation time.In comparison with two other software tools,Loci Scan accommodated missing data,reduced calculation time,and offered more fitness functions.In large datasets,the sample size of training data exerted the strongest influence on calculation time,whereas the marker size of training data showed no effect,and target marker number had limited effect on analysis speed. 展开更多
关键词 Plant variety discrimination Genetic marker combination Variety discrimination power Genetic algorithm
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Impact pressure of waves generated by landslides on bank slopes
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作者 CAO Ting WANG Pingyi +1 位作者 QIU Zhenfeng LIU Jie 《Journal of Mountain Science》 SCIE CSCD 2024年第3期918-931,共14页
Impulse waves that are generated by landslides in narrow reservoir areas threaten the stability of buildings and bank slopes.To discuss the action process and evolution law of the wave pressure on bank slopes,a three-... Impulse waves that are generated by landslides in narrow reservoir areas threaten the stability of buildings and bank slopes.To discuss the action process and evolution law of the wave pressure on bank slopes,a three-dimensional physical model test that considers impulse waves generated by landslides was performed,and factors including landslide width,thickness,slope angles of the sliding surface,and bank slope angle were considered.Based on wave forms on the bank slopes,wave pressure curve characteristics,and peak value,the action process of wave pressure could be divided into the following stages:maximum pulsating pressure stage,wave impact stage(when waves break),and stationary pulsation stage.It was found that wave breaking is dependent on the value of the surf similarity parameterξ.The distribution pattern of impact pressure decays linearly on both sides of the maximum impact pressure point,and the attenuation degree decreases when it attains 40%of the maximum value.Thus,it is proposed that the prediction formula for the maximum effective impact pressure of the bank slope be related to the reciprocal of wave steepness,relative water depth,and slope rate.The prediction formula provides strong theoretical support for early safety warning and for predicting the bank slope under impulse waves generated by landslides. 展开更多
关键词 Model test Impact pressure Action stage Breaking discrimination Distribution model
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The Analysis of the Correlation between SPT and CPT Based on CNN-GA and Liquefaction Discrimination Research
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作者 Ruihan Bai Feng Shen +2 位作者 Zihao Zhao Zhiping Zhang Qisi Yu 《Computer Modeling in Engineering & Sciences》 SCIE EI 2024年第2期1159-1182,共24页
The objective of this study is to investigate themethods for soil liquefaction discrimination. Typically, predicting soilliquefaction potential involves conducting the standard penetration test (SPT), which requires f... The objective of this study is to investigate themethods for soil liquefaction discrimination. Typically, predicting soilliquefaction potential involves conducting the standard penetration test (SPT), which requires field testing and canbe time-consuming and labor-intensive. In contrast, the cone penetration test (CPT) provides a more convenientmethod and offers detailed and continuous information about soil layers. In this study, the feature matrix based onCPT data is proposed to predict the standard penetration test blow count N. The featurematrix comprises the CPTcharacteristic parameters at specific depths, such as tip resistance qc, sleeve resistance f s, and depth H. To fuse thefeatures on the matrix, the convolutional neural network (CNN) is employed for feature extraction. Additionally,Genetic Algorithm (GA) is utilized to obtain the best combination of convolutional kernels and the number ofneurons. The study evaluated the robustness of the proposed model using multiple engineering field data sets.Results demonstrated that the proposed model outperformed conventional methods in predicting N values forvarious soil categories, including sandy silt, silty sand, and clayey silt. Finally, the proposed model was employedfor liquefaction discrimination. The liquefaction discrimination based on the predicted N values was comparedwith the measured N values, and the results showed that the discrimination results were in 75% agreement. Thestudy has important practical application value for foundation liquefaction engineering. Also, the novel methodadopted in this research provides new ideas and methods for research in related fields, which is of great academicsignificance. 展开更多
关键词 CNN liquefaction discrimination SPT CPT
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I-DCGAN and TOPSIS-IFP:A simulation generation model for radiographic flaw detection images in light alloy castings and an algorithm for quality evaluation of generated images
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作者 Ming-jun Hou Hao Dong +7 位作者 Xiao-yuan Ji Wen-bing Zou Xiang-sheng Xia Meng Li Ya-jun Yin Bao-hui Li Qiang Chen Jian-xin Zhou 《China Foundry》 SCIE EI CAS CSCD 2024年第3期239-247,共9页
The intelligent detection technology driven by X-ray images and deep learning represents the forefront of advanced techniques and development trends in flaw detection and automated evaluation of light alloy castings.H... The intelligent detection technology driven by X-ray images and deep learning represents the forefront of advanced techniques and development trends in flaw detection and automated evaluation of light alloy castings.However,the efficacy of deep learning models hinges upon a substantial abundance of flaw samples.The existing research on X-ray image augmentation for flaw detection suffers from shortcomings such as poor diversity of flaw samples and low reliability of quality evaluation.To this end,a novel approach was put forward,which involves the creation of the Interpolation-Deep Convolutional Generative Adversarial Network(I-DCGAN)for flaw detection image generation and a comprehensive evaluation algorithm named TOPSIS-IFP.I-DCGAN enables the generation of high-resolution,diverse simulated images with multiple appearances,achieving an improvement in sample diversity and quality while maintaining a relatively lower computational complexity.TOPSIS-IFP facilitates multi-dimensional quality evaluation,including aspects such as diversity,authenticity,image distribution difference,and image distortion degree.The results indicate that the X-ray radiographic images of magnesium and aluminum alloy castings achieve optimal performance when trained up to the 800th and 600th epochs,respectively.The TOPSIS-IFP value reaches 78.7%and 73.8%similarity to the ideal solution,respectively.Compared to single index evaluation,the TOPSIS-IFP algorithm achieves higher-quality simulated images at the optimal training epoch.This approach successfully mitigates the issue of unreliable quality associated with single index evaluation.The image generation and comprehensive quality evaluation method developed in this paper provides a novel approach for image augmentation in flaw recognition,holding significant importance for enhancing the robustness of subsequent flaw recognition networks. 展开更多
关键词 light alloy casting flaw detection image generator DISCRIMINATOR comprehensive evaluation index
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Bearing Fault Diagnosis Based on Deep Discriminative Adversarial Domain Adaptation Neural Networks
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作者 Jinxi Guo Kai Chen +5 位作者 Jiehui Liu Yuhao Ma Jie Wu Yaochun Wu Xiaofeng Xue Jianshen Li 《Computer Modeling in Engineering & Sciences》 SCIE EI 2024年第3期2619-2640,共22页
Intelligent diagnosis driven by big data for mechanical fault is an important means to ensure the safe operation ofequipment. In these methods, deep learning-based machinery fault diagnosis approaches have received in... Intelligent diagnosis driven by big data for mechanical fault is an important means to ensure the safe operation ofequipment. In these methods, deep learning-based machinery fault diagnosis approaches have received increasingattention and achieved some results. It might lead to insufficient performance for using transfer learning alone andcause misclassification of target samples for domain bias when building deep models to learn domain-invariantfeatures. To address the above problems, a deep discriminative adversarial domain adaptation neural networkfor the bearing fault diagnosis model is proposed (DDADAN). In this method, the raw vibration data are firstlyconverted into frequency domain data by Fast Fourier Transform, and an improved deep convolutional neuralnetwork with wide first-layer kernels is used as a feature extractor to extract deep fault features. Then, domaininvariant features are learned from the fault data with correlation alignment-based domain adversarial training.Furthermore, to enhance the discriminative property of features, discriminative feature learning is embeddedinto this network to make the features compact, as well as separable between classes within the class. Finally, theperformance and anti-noise capability of the proposedmethod are evaluated using two sets of bearing fault datasets.The results demonstrate that the proposed method is capable of handling domain offset caused by differentworkingconditions and maintaining more than 97.53% accuracy on various transfer tasks. Furthermore, the proposedmethod can achieve high diagnostic accuracy under varying noise levels. 展开更多
关键词 Fault diagnosis transfer learning domain adaptation discriminative feature learning correlation alignment
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A Dual Discriminator Method for Generalized Zero-Shot Learning
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作者 Tianshu Wei Jinjie Huang 《Computers, Materials & Continua》 SCIE EI 2024年第4期1599-1612,共14页
Zero-shot learning enables the recognition of new class samples by migrating models learned from semanticfeatures and existing sample features to things that have never been seen before. The problems of consistencyof ... Zero-shot learning enables the recognition of new class samples by migrating models learned from semanticfeatures and existing sample features to things that have never been seen before. The problems of consistencyof different types of features and domain shift problems are two of the critical issues in zero-shot learning. Toaddress both of these issues, this paper proposes a new modeling structure. The traditional approach mappedsemantic features and visual features into the same feature space;based on this, a dual discriminator approachis used in the proposed model. This dual discriminator approach can further enhance the consistency betweensemantic and visual features. At the same time, this approach can also align unseen class semantic features andtraining set samples, providing a portion of information about the unseen classes. In addition, a new feature fusionmethod is proposed in the model. This method is equivalent to adding perturbation to the seen class features,which can reduce the degree to which the classification results in the model are biased towards the seen classes.At the same time, this feature fusion method can provide part of the information of the unseen classes, improvingits classification accuracy in generalized zero-shot learning and reducing domain bias. The proposed method isvalidated and compared with othermethods on four datasets, and fromthe experimental results, it can be seen thatthe method proposed in this paper achieves promising results. 展开更多
关键词 Generalized zero-shot learning modality consistent DISCRIMINATOR domain shift problem feature fusion
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Artificial neural network-based method for discriminating Compton scattering events in high-purity germaniumγ-ray spectrometer
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作者 Chun-Di Fan Guo-Qiang Zeng +5 位作者 Hao-Wen Deng Lei Yan Jian Yang Chuan-Hao Hu Song Qing Yang Hou 《Nuclear Science and Techniques》 SCIE EI CAS CSCD 2024年第2期64-84,共21页
To detect radioactive substances with low activity levels,an anticoincidence detector and a high-purity germanium(HPGe)detector are typically used simultaneously to suppress Compton scattering background,thereby resul... To detect radioactive substances with low activity levels,an anticoincidence detector and a high-purity germanium(HPGe)detector are typically used simultaneously to suppress Compton scattering background,thereby resulting in an extremely low detection limit and improving the measurement accuracy.However,the complex and expensive hardware required does not facilitate the application or promotion of this method.Thus,a method is proposed in this study to discriminate the digital waveform of pulse signals output using an HPGe detector,whereby Compton scattering background is suppressed and a low minimum detectable activity(MDA)is achieved without using an expensive and complex anticoincidence detector and device.The electric-field-strength and energy-deposition distributions of the detector are simulated to determine the relationship between pulse shape and energy-deposition location,as well as the characteristics of energy-deposition distributions for fulland partial-energy deposition events.This relationship is used to develop a pulse-shape-discrimination algorithm based on an artificial neural network for pulse-feature identification.To accurately determine the relationship between the deposited energy of gamma(γ)rays in the detector and the deposition location,we extract four shape parameters from the pulse signals output by the detector.Machine learning is used to input the four shape parameters into the detector.Subsequently,the pulse signals are identified and classified to discriminate between partial-and full-energy deposition events.Some partial-energy deposition events are removed to suppress Compton scattering.The proposed method effectively decreases the MDA of an HPGeγ-energy dispersive spectrometer.Test results show that the Compton suppression factors for energy spectra obtained from measurements on ^(152)Eu,^(137)Cs,and ^(60)Co radioactive sources are 1.13(344 keV),1.11(662 keV),and 1.08(1332 keV),respectively,and that the corresponding MDAs are 1.4%,5.3%,and 21.6%lower,respectively. 展开更多
关键词 High-purity germaniumγ-ray spectrometer Pulse-shape discrimination Compton scattering Artificial neural network Minimum detectable activity
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High-Precision Doppler Frequency Estimation Based Positioning Using OTFS Modulations by Red and Blue Frequency Shift Discriminator
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作者 Shaojing Wang Xiaomei Tang +3 位作者 Jing Lei Chunjiang Ma Chao Wen Guangfu Sun 《China Communications》 SCIE CSCD 2024年第2期17-31,共15页
Orthogonal Time Frequency and Space(OTFS) modulation is expected to provide high-speed and ultra-reliable communications for emerging mobile applications, including low-orbit satellite communications. Using the Dopple... Orthogonal Time Frequency and Space(OTFS) modulation is expected to provide high-speed and ultra-reliable communications for emerging mobile applications, including low-orbit satellite communications. Using the Doppler frequency for positioning is a promising research direction on communication and navigation integration. To tackle the high Doppler frequency and low signal-to-noise ratio(SNR) in satellite communication, this paper proposes a Red and Blue Frequency Shift Discriminator(RBFSD) based on the pseudo-noise(PN) sequence.The paper derives that the cross-correlation function on the Doppler domain exhibits the characteristic of a Sinc function. Therefore, it applies modulation onto the Delay-Doppler domain using PN sequence and adjusts Doppler frequency estimation by red-shifting or blue-shifting. Simulation results show that the performance of Doppler frequency estimation is close to the Cramér-Rao Lower Bound when the SNR is greater than -15dB. The proposed algorithm is about 1/D times less complex than the existing PN pilot sequence algorithm, where D is the resolution of the fractional Doppler. 展开更多
关键词 channel estimation communication and navigation integration Orthogonal Time Frequency and Space pseudo-noise sequence red-blue frequency shift discriminator
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The Alzheimer’s Dementia Patients’ Observed Illness Course and Experience in Ghana and Care Lessons to Be Learnt: A Mental Health Professional’s Perspective
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作者 Albert M. E. Coleman 《Open Journal of Psychiatry》 2024年第2期91-106,共16页
Alzheimer’s disease (AD) and associated dementia patient numbers continue to increase globally with associated economic costs to healthcare systems. Of note is the increase in numbers in lower and middle-income count... Alzheimer’s disease (AD) and associated dementia patient numbers continue to increase globally with associated economic costs to healthcare systems. Of note is the increase in numbers in lower and middle-income countries (LMICs) including Sub-Saharan African (SSA) countries, which already face challenges with their health budgets from communicable and non-communicable diseases. Ghana, an SSA country, faces the problem of healthcare budgetary difficulties and the additional impact of AD as a consequence of increasing population strata of old aged persons (OAPs) due to the demographic transition effect. This article uses examples of known patients’ illness courses to give a perspective on the lived experience of patients with dementia (PWD) in Ghana, living amongst a populace with a culture of stigmatization of PWD, and a relatively fragile public mental health system (PMHS) for those with mental illness, including AD. The lived experience of AD patients is characterised by stigmatisation, discrimination, non-inclusiveness, diminished dignity and human rights abuses in the face of their mental disability, and eventually death. This article is an advocacy article giving voice to the voiceless and all persons suffering from AD and other dementias in Ghana, whilst pleading for a call to action from healthcare professionals and responsible state agencies. 展开更多
关键词 Alzheimer’s Dementia PATIENTS Ghana STIGMATIZATION Discrimination Human Rights DIGNITY
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The Influence of Price Discrimination from Airports on the Route Development Behavior of Airlines
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作者 Daniel Schnitzler 《Journal of Civil Engineering and Architecture》 2024年第1期17-29,共13页
Given the prominence and magnitude of airport incentive schemes,it is surprising that literature hitherto remains silent as to their effectiveness.In this paper,the relationship between airport incentive schemes and t... Given the prominence and magnitude of airport incentive schemes,it is surprising that literature hitherto remains silent as to their effectiveness.In this paper,the relationship between airport incentive schemes and the route development behavior of airlines is analyzed.Because of rare and often controversial findings in the extant literature regarding relevant influencing variables for attracting airlines at an airport,expert interviews are used as a complement to formulate testable hypotheses in this regard.A fixed effects regression model is used to test the hypotheses with a dataset that covers all seat capacity offered at the 22 largest German commercial airports in the week 46 from 2004 to 2011.It is found that incentives from primary choice,as well as secondary choice airports,have a significant influence on Low Cost Carriers.Furthermore,Low Cost Carriers,in general,do not leave any of both types of airports when the incentives cease.In the case of Network Carriers,no case is found where one joins a primary choice airport and receives an incentive.Insufficient data between Network Carriers and secondary choice airports in the time when incentives have ceased means that no statement can be given. 展开更多
关键词 AIRLINE rout development price discrimination
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Analysis of Serum Metabolic Profile by Ultra-performance Liquid Chromatography-mass Spectrometry for Biomarkers Discovery: Application in a Pilot Study to Discriminate Patients with Tuberculosis 被引量:6
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作者 Shuang Feng Yan-Qing Du +3 位作者 Li Zhang LeiZhang Ran-Ran Feng Shu-Ye Liu 《Chinese Medical Journal》 SCIE CAS CSCD 2015年第2期159-168,共10页
Background:Tuberculosis (TB) is a chronic wasting inflammatory disease characterized by multisystem involvement,which can cause metabolic derangements in afflicted patients.Metabolic signatures have been exploited ... Background:Tuberculosis (TB) is a chronic wasting inflammatory disease characterized by multisystem involvement,which can cause metabolic derangements in afflicted patients.Metabolic signatures have been exploited in the study of several diseases.However,the serum that is successfully used in TB diagnosis on the basis of metabolic profiling is not by much.Methods:Orthogonal partial least-squares discriminant analysis was capable of distinguishing TB patients from both healthy subjects and patients with conditions other than TB.Therefore,TB-specific metabolic profiling was established.Clusters of potential biomarkers for differentiating TB active from non-TB diseases were identified using Mann-Whitney U-test.Multiple logistic regression analysis of metabolites was calculated to determine the suitable biomarker group that allows the efficient differentiation of patients with TB active from the control subjects.Results:From among 271 participants,12 metabolites were found to contribute to the distinction between the TB active group and the control groups.These metabolites were mainly involved in the metabolic pathways of the following three biomolecules:Fatty acids,amino acids,and lipids.The receiver operating characteristic curves of3D,7D,and 11D-phytanic acid,behenic acid,and threoninyl-γ-glutamate exhibited excellent efficiency with area under the curve (AUC) values of 0.904 (95% confidence interval [CI]:0.863-0.944),0.93 (95% CI:0.893-0.966),and 0.964 (95% CI:0.941-0.988),respectively.The largest and smallest resulting AUCs were 0.964 and 0.720,indicating that these biomarkers may be involved in the disease mechanisms.The combination of lysophosphatidylcholine (18∶0),behenic acid,threoninyl-γ-glutamate,and presqualene diphosphate was used to represent the most suitable biomarker group for the differentiation of patients with TB active from the control subjects,with an AUC value of 0.991.Conclusion:The metabolic analysis results identified new serum biomarkers that can distinguish TB from non-TB diseases.The metabolomics-based analysis provides specific insights into the biology of TB and may offer new avenues for TB diagnosis. 展开更多
关键词 METABOLITES Orthogonal Partial Least-squares Discriminant Analysis SERUM TUBERCULOSIS Ultra-performance LiquidChromatography-mass Spectrometry
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Establishment of a Multi-color Genomic in situ Hybridization Technique to Simultaneously Discriminate the Three Interspecific Hybrid Genomes in Gossypium 被引量:4
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作者 Bing Guan Kai Wang Bao-Liang Zhou Wang-Zhen Guo Tian-Zhen Zhang 《Journal of Integrative Plant Biology》 SCIE CAS CSCD 2008年第3期345-351,共7页
To identify alien chromosomes in recipient progenies and to analyze genome components in polyploidy, a genomic in situ hybridization (GISH) technique that is suitable for cotton was developed using increased stringe... To identify alien chromosomes in recipient progenies and to analyze genome components in polyploidy, a genomic in situ hybridization (GISH) technique that is suitable for cotton was developed using increased stringency conditions. The increased stringency conditions were a combination of the four factors in the following optimized state: 100:1 ratio of blocking DNA to probe, 60% formamide wash solution, 43 ℃ temperature wash and a 13 min wash. Under these specific conditions using gDNA from Gossypium sturtianum (C1 C1 ) as a probe, strong hybridization signals were only observed on chromosomes from the C1 genome in somatic cells of the hybrid F1 (G. hirsutum x G. sturtianum) (AtDtC1). Therefore, GISH was able to discriminate parental chromosomes in the hybrid. Further, we developed a multi-color GISH to simultaneously discriminate the three genomes of the above hybrid. The results repeatedly displayed the three genomes, At, Dt, and C1, and each set of chromosomes with a unique color, making them easy to identify. The power of the multi-color GISH was proven by analysis of the hexaploid hybrid F1 (G. hirsutum x G. australe) (AtAtDtDtG2G2). We believe that the powerful multi-color GISH technique could be applied extensively to analyze the genome component in polyploidy and to identify alien chromosomes in the recipient progenies. 展开更多
关键词 alien chromosome cotton discrimination genome interspecific hybrid multi-color genomic in situ hybridization stringency
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Conceptual design of a Cs2LiLaBr6 scintillator‑based neutron total cross section spectrometer on the back‑n beam line at CSNS 被引量:2
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作者 Da‑Jun Zhao Song Feng +6 位作者 Pin‑Jing Cheng Rong Liu Wen Luo Hao‑Qiang Wang Jie‑Ming Xue Kun Zhu Bo Zheng 《Nuclear Science and Techniques》 SCIE EI CAS CSCD 2023年第1期23-32,共10页
To reduce the experimental uncertainty in the 235 U resonance energy region and improve the detection efficiency for neutron total cross section measurements compared with those obtained with the neutron total cross s... To reduce the experimental uncertainty in the 235 U resonance energy region and improve the detection efficiency for neutron total cross section measurements compared with those obtained with the neutron total cross section spectrometer(NTOX), a dedicated lithium-containing scintillation detector has been developed on the Back-n beam line at the China Spallation Neutron Source. The Fast Scintillator-based Neutron Total Cross Section(FAST) spectrometer has been designed based on a Cs2Li La Br6(CLLB) scintillator considering the γ-ray flash and neutron environment on the Back-n beam line. The response of the CLLB scintillator to neutrons and γ-rays was evaluated with different 6Li/7 Li abundance ratios using Geant4. The neutron-γdiscrimination performance of the CLLB has been simulated considering different scintillation parameters, physical designs,and light readout modes. A cubic 6Li-enriched( > 90%) CLLB scintillator, which has a thickness of 4-9 mm and side length of no less than 50 mm to cover the Φ 50 mm neutron beam at the spectrometer position, has been proposed coupling to a side readout SiPM array to construct the FAST spectrometer. The developed simulation techniques for neutron-γ discrimination performance could provide technical support for other neutron-induced reaction measurements on the Back-n beam line. 展开更多
关键词 Neutron total cross section CLLB scintillator GEANT4 Pulse shape discrimination(PSD)
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Methylome and transcriptome data integration reveals potential roles of DNA methylation and candidate biomarkers of cow Streptococcus uberis subclinical mastitis 被引量:2
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作者 Mengqi Wang Nathalie Bissonnette +6 位作者 Mario Laterriere Pier‑Luc Dudemaine David Gagne Jean‑Philippe Roy Xin Zhao Marc‑Andre Sirard Eveline M.Ibeagha‑Awemu 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2023年第2期593-613,共21页
Background:Mastitis caused by different pathogens including Streptococcus uberis(S.uberis)is responsible for huge economic losses to the dairy industry.In order to investigate the potential genetic and epigenetic regu... Background:Mastitis caused by different pathogens including Streptococcus uberis(S.uberis)is responsible for huge economic losses to the dairy industry.In order to investigate the potential genetic and epigenetic regulatory mecha‑nisms of subclinical mastitis due to S.uberis,the DNA methylome(whole genome DNA methylation sequencing)and transcriptome(RNA sequencing)of milk somatic cells from cows with naturally occurring S.uberis subclinical mastitis and healthy control cows(n=3/group)were studied.Results:Globally,the DNA methylation levels of CpG sites were low in the promoters and first exons but high in inner exons and introns.The DNA methylation levels at the promoter,first exon and first intron regions were nega‑tively correlated with the expression level of genes at a whole‑genome‑wide scale.In general,DNA methylation level was lower in S.uberis‑positive group(SUG)than in the control group(CTG).A total of 174,342 differentially methylated cytosines(DMCs)(FDR<0.05)were identified between SUG and CTG,including 132,237,7412 and 34,693 DMCs in the context of CpG,CHG and CHH(H=A or T or C),respectively.Besides,101,612 methylation haplotype blocks(MHBs)were identified,including 451 MHBs that were significantly different(dMHB)between the two groups.A total of 2130 differentially expressed(DE)genes(1378 with up‑regulated and 752 with down‑regulated expression)were found in SUG.Integration of methylome and transcriptome data with MethGET program revealed 1623 genes with signifi‑cant changes in their methylation levels and/or gene expression changes(MetGDE genes,MethGET P‑value<0.001).Functional enrichment of genes harboring≥15 DMCs,DE genes and MetGDE genes suggest significant involvement of DNA methylation changes in the regulation of the host immune response to S.uberis infection,especially cytokine activities.Furthermore,discriminant correlation analysis with DIABLO method identified 26 candidate biomarkers,including 6 DE genes,15 CpG‑DMCs and 5 dMHBs that discriminated between SUG and CTG.Conclusion:The integration of methylome and transcriptome of milk somatic cells suggests the possible involve‑ment of DNA methylation changes in the regulation of the host immune response to subclinical mastitis due to S.uberis.The presented genetic and epigenetic biomarkers could contribute to the design of management strategies of subclinical mastitis and breeding for mastitis resistance. 展开更多
关键词 Discriminant biomarkers Gene expression Genome‑wide DNA methylation pattern Immune processes and pathways Methylation haplotype block Milk somatic cell Streptococcus uberis Subclinical mastitis
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Egg recognition and nestling discrimination in the Crested Myna(Acridotheres cristatellus):Size matters 被引量:1
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作者 Jinmei Liu Fangfang Zhang +1 位作者 Yuran Liu Wei Liang 《Avian Research》 SCIE CSCD 2023年第3期492-498,共7页
Most studies exploring abilities of hosts to detect brood parasitism are based on detecting colour and/or pattern differences among parasitic and host eggs or nestlings,while only few were focused on size differences.... Most studies exploring abilities of hosts to detect brood parasitism are based on detecting colour and/or pattern differences among parasitic and host eggs or nestlings,while only few were focused on size differences.True recognition and recognition by discordancy are used to explain cognitive mechanisms of host egg recognition;however,only a few studies have found that hosts use recognition by discordancy.This study investigated:1)whether egg and nestling recognitions in the Crested Myna(Acridotheres cristatellus) are based on size cues;2)whether the egg cognitive mechanism is recognition by discordancy based on size cues;and 3) whether the longer the experiment time,the higher the egg recognition rate.Our results showed that the Crested Myna uses egg or nestling size as a recognition cue while the egg and nestling colour and patterning are not associated with egg or nestling rejection,thus the cognitive mechanism of egg recognition in the Crested Myna is recognition by discordancy based on egg size cues.Furthermore,there is a rejection delay in time of egg rejection behaviour of the Crested Myna.Therefore,we suggest that the periodicity of egg rejection experiments could be appropriately extended,especially for species with relatively low egg recognition ability. 展开更多
关键词 Discordancy recognition Egg rejection Nest sanitation behaviour Nestling discrimination Rejection delay
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