We collected the data on the Sendeng-4 chemical composition corresponding targets through the literature and from Drug Bank, SuperT arget, TTD(Therapeutic Targets Database) and other databases and the relevant signali...We collected the data on the Sendeng-4 chemical composition corresponding targets through the literature and from Drug Bank, SuperT arget, TTD(Therapeutic Targets Database) and other databases and the relevant signaling pathways from the KEGG(Kyoto Encyclopedia of Genes and Genomes) database and established models of the chemical composition-target network and chemical composition-target- disease network using Cytoscape software, the analysis indicated that the chemical composition had at least nine different types of targets that acted together to exert effects on the diseases, suggesting a "multi-component, multi-target" feature of the traditional Mongolian medicine. We also employed the rat model of rheumatoid arthritis induced by Collgen Type II to validate the key targets of the chemical components of Sendeng-4, and three of the key targets were validated through laboratory experiments, further confirming the anti-inflammatory effects of Sendeng-4. In all, this study predicted the active ingredients and targets of Sendeng-4, and explored its mechanism of action, which provided new strategies and methods for further research and development of Sendeng-4 and other traditional Mongolian medicines as well.展开更多
基金supported by the National Natural Science Foundation of China(No.81160550)Inner Mongolia Natural Science Foundation(No.2013JQ03)2010 Science and Technology Project of social development in Inner Mongolia
文摘We collected the data on the Sendeng-4 chemical composition corresponding targets through the literature and from Drug Bank, SuperT arget, TTD(Therapeutic Targets Database) and other databases and the relevant signaling pathways from the KEGG(Kyoto Encyclopedia of Genes and Genomes) database and established models of the chemical composition-target network and chemical composition-target- disease network using Cytoscape software, the analysis indicated that the chemical composition had at least nine different types of targets that acted together to exert effects on the diseases, suggesting a "multi-component, multi-target" feature of the traditional Mongolian medicine. We also employed the rat model of rheumatoid arthritis induced by Collgen Type II to validate the key targets of the chemical components of Sendeng-4, and three of the key targets were validated through laboratory experiments, further confirming the anti-inflammatory effects of Sendeng-4. In all, this study predicted the active ingredients and targets of Sendeng-4, and explored its mechanism of action, which provided new strategies and methods for further research and development of Sendeng-4 and other traditional Mongolian medicines as well.
