Background:To study the effects of the main diterpene esters in Euphorbia factor L_(1),L_(2),L_(3),L_(7a),L_(7b)and L_(8)on the transcriptional activity and protein expression of liver X receptor(LXR).Methods:The effe...Background:To study the effects of the main diterpene esters in Euphorbia factor L_(1),L_(2),L_(3),L_(7a),L_(7b)and L_(8)on the transcriptional activity and protein expression of liver X receptor(LXR).Methods:The effect of the main diterpene ester components in Semen Euphorbiae on the viability of HEK293 cells were studied by MTT assay.The LXR-Luc plasmid vector was transfected into HEK293 cells and treated with Euphorbia factor L_(1),L_(2),L_(3),L_(7a),L_(7b)and L_(8)for 24 h.The effect of the main diterpene ester components of Semen Euphorbiae on LXR-Luc luciferase activity was investigated by dual luciferase reporter gene system,and the expression of LXRαprotein was detected by Western Blot.Results:Euphorbia factor L_(1),L_(2),L_(3),L_(7a),L_(7b)and L_(8)could significantly reduce the relative luciferase activity(RLU)of LXRα,and the expression level of LXRαprotein was significantly down-regulated.Conclusion:Euphorbia factor L_(1),L_(2),L_(3),L_(7a),L_(7b)and L_(8)can inhibit the expression of LXR protein level,which may be achieved by inhibiting the transcriptional activity of LXR.展开更多
A new abietane diterpene 12-hydroxy-7-oxo-5, 8, 11, 13-tetraene-18, 6-abietanolide, together with a new natural sterol stigmasta-7, 22-diene-3β, 5α 6α-triol have been isolated from the fruiting body of the fungus P...A new abietane diterpene 12-hydroxy-7-oxo-5, 8, 11, 13-tetraene-18, 6-abietanolide, together with a new natural sterol stigmasta-7, 22-diene-3β, 5α 6α-triol have been isolated from the fruiting body of the fungus Phellinus igniarius. Their structures were elucidated by spectroscopic methods including 2D NMR techniques.展开更多
Two novel diterpenes, Euphorprolitherin A (1) and Euphorprolitherin B (2), were isolated from the roots of Euphorbia prolifera. Their structures were elucidated on the basis of spectroscopic methods.
A new cembranoide deterpene was isolated from the soft coral Sinularia Tenella . The crystal and chemical structure of the title compound were determined by means of spectroscopic methods and X ray diffraction ana...A new cembranoide deterpene was isolated from the soft coral Sinularia Tenella . The crystal and chemical structure of the title compound were determined by means of spectroscopic methods and X ray diffraction analysis as ( 1R *,4R *,5S *,12S *,12R * ) 9 acetoxy cembr 8\%E\%,15(17) dien 16,4 olide. It shows a moderate cytotoxicity against P 388 and L 1210 cell lines.展开更多
Two new diterpenes, 15-ethyl-18-methyl pinifolate (1) and 18-hydroxy-labda-8(17), 13E-dien-15-acetate (2), were isolated from the needles of Pinus sylvestris. Their structures were elucidated by spectroscopic me...Two new diterpenes, 15-ethyl-18-methyl pinifolate (1) and 18-hydroxy-labda-8(17), 13E-dien-15-acetate (2), were isolated from the needles of Pinus sylvestris. Their structures were elucidated by spectroscopic methods, including 2D-NMR spectra. Compound 1 exhibited the significant cytotoxic activity against the human carcinoma cell lines Hela, SK-N-SH and BEL-7402 in vitro.展开更多
One new grayanane diterpene glucoside, rhodomoside A 1 was isolated from the roots of Rhododendron molle G. Don (Ericaccae). Its structure was elucidated on the basis of spectral analysis.
Three new diterpene alkaloids,tangutidines A-C(1-3),and four known alkaloids(4-7)were isolated from the whole plant of Aconitum tanguticum,from which amphoteric diterpene alkaloids(1-3)were obtained for the first time...Three new diterpene alkaloids,tangutidines A-C(1-3),and four known alkaloids(4-7)were isolated from the whole plant of Aconitum tanguticum,from which amphoteric diterpene alkaloids(1-3)were obtained for the first time.The structures of 1-3 were elucidated by detailed interpretation of spectroscopic data,including MS and NMR data.All of them were evaluated for their cytotoxic activities.展开更多
Chemical investigation on the ethanol extract from the whole plants of Delphinium chrysotrichum resulted in the isolation of two new diterpene alkaloids named delphatisine A (1) and delphatisine B (2), respectivel...Chemical investigation on the ethanol extract from the whole plants of Delphinium chrysotrichum resulted in the isolation of two new diterpene alkaloids named delphatisine A (1) and delphatisine B (2), respectively. The structures of the new compounds were deduced on the basis of their spectral data (IR, HREIMS, EIMS, 1 D, 2D-NMR). This is the first report on the isolation of diterpenoid alkaloids from the D. chrysotrichum.展开更多
A new daphnane diterpene was isolated from the root barks of Daphne tangutica Maxim. Its structure was elucidated as 1, 2α-dihydro-20-palimoyldaphnctoxin by the spectroscopic evidence including 2D-NMR.
A new isopimarane diterpene, isopimar-15-en-3 beta,8 beta,20-triol, was isolated from Nepeta prattii. Its structure was elucidated by spectral methods (EIMS, 1D and 2D NMR).
Euphorbia ebracteolata Hayata (E. ebracteolata) is a Chinese herbal medicine used for the treatment of tumor diseases. An ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry (UPLC-Q-...Euphorbia ebracteolata Hayata (E. ebracteolata) is a Chinese herbal medicine used for the treatment of tumor diseases. An ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS) based chemical profiling approach was established for the rapid separation and characterization on phloroglucinol derivatives and diterpenes in E. ebracteolata. Three phloroglucinol derivatives and nine diterpenes were identified by exact mass measurement and were further confirmed by Ms2 data. In addition, the chemical profiles of six compounds were acquired by reference standards. Furthermore, the fragmentation rules of phloroglucinol derivatives and diterpenes of E. ebracteolata were analyzed, and each chromatographic peak was classified.展开更多
Chemical investigation on the ethanol extract from the roots of Spiraea japonica var. acuta resulted in the isolation of two new diterpene alkaloids named spiratines A and B (1-2), respectively.
In this study, ent-Kaurane-3β,16β,17-trioi (1) and ent-kaurane-2a,16β,17-triol (2), were isolated from the leaves ofRubus corchorifolius L. f. Their structures were elucidated based on extensive spectroscopic a...In this study, ent-Kaurane-3β,16β,17-trioi (1) and ent-kaurane-2a,16β,17-triol (2), were isolated from the leaves ofRubus corchorifolius L. f. Their structures were elucidated based on extensive spectroscopic analysis. NMR experiments identified the two compounds and X-ray crystallographic analysis confirmed their crystal structures. The crystal of 1 belongs to monoclinic with space group P21 and a = 10.7641(3), b = 7.2789(3), c = 11.7862(4) A, β = 95.275(3)°, V = 919.55(6) A3, Z = 2, C20H34O4, Mr = 322.49 g/mol, Dc = 1.230 g/m3, F(000) = 376, 2 = 1.54178 A,μ = 0.661 mm-1, R = 0.0319, and wR = 0.0811 for 10889 observed reflections (I〉 2σ(I)). The crystal of 2 is classified as orthorhombic with space group P212121 and a = 10.7641(3), b = 12.72224(19), c = 21.3929(3) A, V= 1748.94(4) A3, Z= 4, C20H3404, Mr = 322.47, De = 1.225 Mg/m3 F(000) = 712, λ = 1.54184 A,μ = 0.625 mm-1, R = 0.0303 and wR = 0.0759 for 10470 observed reflections (1〉 2σ(I)). Meanwhile, the compound revealed low inhibitory activities toward HepG2, MCF-7, and SCG-7901 cells.展开更多
Five new diterpenes were isolated from Euphorbia sieboldiana and identified as atis-16-en-13(S)-hydroxy-3,14-dione(Ⅰ),atis-16-en- 14-oxo-13(S),3 β-diol(Ⅱ),atisane-3-oxo-16α,17-diol(Ⅳ),atisane-3β ,16α,17-triol(...Five new diterpenes were isolated from Euphorbia sieboldiana and identified as atis-16-en-13(S)-hydroxy-3,14-dione(Ⅰ),atis-16-en- 14-oxo-13(S),3 β-diol(Ⅱ),atisane-3-oxo-16α,17-diol(Ⅳ),atisane-3β ,16α,17-triol(Ⅴ)and atisane-3β-acetyl-16α,17-diol(Ⅵ)based on the chemical,spectral evidences and X-ray diffraction.展开更多
s: Two new diterpene alkaloids named swinanine C and D were isolated from Delphinium swinanense var leptopogon (H. -M. ) W. T. Wang. Their structures were elucidated by means of spectral methods , principally 1H-NHR, ...s: Two new diterpene alkaloids named swinanine C and D were isolated from Delphinium swinanense var leptopogon (H. -M. ) W. T. Wang. Their structures were elucidated by means of spectral methods , principally 1H-NHR, DEPT and 13CNMR techniques.展开更多
The antibacierial (staphylococcus aureus) activities of 23 diterpene quinones from the Salvia przewalskii Maxim, the artificial synthesis ones and that of the metabolism in pigs were examined in an effort to study the...The antibacierial (staphylococcus aureus) activities of 23 diterpene quinones from the Salvia przewalskii Maxim, the artificial synthesis ones and that of the metabolism in pigs were examined in an effort to study the quantitative structure activity relationship with the Free-Wilson method. With the application of the multipiy regression, the Free-Wilson pattern was established by the dummy (indicator) variable of the structural fragments or substituents. The results showed: (1) The diketone moiety played the fundamental role in antibacterial activities, ortho-quinones was higher active than para-quinones. (2) The single bond existed between the C15 and the C16 at D-ring led to higher activity. (3) The saturation at the A-ring led to higher activity. (4) The components adjacent to nitrogen substituents at C15 led to higher activity. (5)The hydroxylation or dehydrogenation at A-ring led to less activity. The research results can provide the theoretical basis for developing a new antibacterial drug from the Salvia diterpene quinones.展开更多
基金supported by National Natural Science Foundation of China(Grant No.82074021).
文摘Background:To study the effects of the main diterpene esters in Euphorbia factor L_(1),L_(2),L_(3),L_(7a),L_(7b)and L_(8)on the transcriptional activity and protein expression of liver X receptor(LXR).Methods:The effect of the main diterpene ester components in Semen Euphorbiae on the viability of HEK293 cells were studied by MTT assay.The LXR-Luc plasmid vector was transfected into HEK293 cells and treated with Euphorbia factor L_(1),L_(2),L_(3),L_(7a),L_(7b)and L_(8)for 24 h.The effect of the main diterpene ester components of Semen Euphorbiae on LXR-Luc luciferase activity was investigated by dual luciferase reporter gene system,and the expression of LXRαprotein was detected by Western Blot.Results:Euphorbia factor L_(1),L_(2),L_(3),L_(7a),L_(7b)and L_(8)could significantly reduce the relative luciferase activity(RLU)of LXRα,and the expression level of LXRαprotein was significantly down-regulated.Conclusion:Euphorbia factor L_(1),L_(2),L_(3),L_(7a),L_(7b)and L_(8)can inhibit the expression of LXR protein level,which may be achieved by inhibiting the transcriptional activity of LXR.
基金Financial support was provided by the Science Foundation for Postdoctoral Program of China(Grant No.2003034251)NCET,and the National Natural Science Foundation of China(NNSFC,Grant No.20432030 and 30400586).We thank Professor K.L.Chen for her help of the fungal material collection.
文摘A new abietane diterpene 12-hydroxy-7-oxo-5, 8, 11, 13-tetraene-18, 6-abietanolide, together with a new natural sterol stigmasta-7, 22-diene-3β, 5α 6α-triol have been isolated from the fruiting body of the fungus Phellinus igniarius. Their structures were elucidated by spectroscopic methods including 2D NMR techniques.
基金We wish to thank the Teaching and Research Award Program for Outstanding Young Teachers in Higher Education Institutions of the Ministry of Education and the Med-X Foundation from Fudan University.
文摘Two novel diterpenes, Euphorprolitherin A (1) and Euphorprolitherin B (2), were isolated from the roots of Euphorbia prolifera. Their structures were elucidated on the basis of spectroscopic methods.
文摘A new cembranoide deterpene was isolated from the soft coral Sinularia Tenella . The crystal and chemical structure of the title compound were determined by means of spectroscopic methods and X ray diffraction analysis as ( 1R *,4R *,5S *,12S *,12R * ) 9 acetoxy cembr 8\%E\%,15(17) dien 16,4 olide. It shows a moderate cytotoxicity against P 388 and L 1210 cell lines.
文摘Two new diterpenes, 15-ethyl-18-methyl pinifolate (1) and 18-hydroxy-labda-8(17), 13E-dien-15-acetate (2), were isolated from the needles of Pinus sylvestris. Their structures were elucidated by spectroscopic methods, including 2D-NMR spectra. Compound 1 exhibited the significant cytotoxic activity against the human carcinoma cell lines Hela, SK-N-SH and BEL-7402 in vitro.
基金the National Natural Science Foundation of China(Grant No.30170104)
文摘One new grayanane diterpene glucoside, rhodomoside A 1 was isolated from the roots of Rhododendron molle G. Don (Ericaccae). Its structure was elucidated on the basis of spectral analysis.
基金the Second Tibetan Plateau Scientific Expedition and Research(STEP)program(2019QZKK0502)the National Natural Science Foundation of China(No.81673329)Authors are grateful for Dr.Ming Zhang from northwest institute of plateau biology,Chinese Academy of Sciences,for the assistance for obtaining plant materials.
文摘Three new diterpene alkaloids,tangutidines A-C(1-3),and four known alkaloids(4-7)were isolated from the whole plant of Aconitum tanguticum,from which amphoteric diterpene alkaloids(1-3)were obtained for the first time.The structures of 1-3 were elucidated by detailed interpretation of spectroscopic data,including MS and NMR data.All of them were evaluated for their cytotoxic activities.
文摘Chemical investigation on the ethanol extract from the whole plants of Delphinium chrysotrichum resulted in the isolation of two new diterpene alkaloids named delphatisine A (1) and delphatisine B (2), respectively. The structures of the new compounds were deduced on the basis of their spectral data (IR, HREIMS, EIMS, 1 D, 2D-NMR). This is the first report on the isolation of diterpenoid alkaloids from the D. chrysotrichum.
文摘A new daphnane diterpene was isolated from the root barks of Daphne tangutica Maxim. Its structure was elucidated as 1, 2α-dihydro-20-palimoyldaphnctoxin by the spectroscopic evidence including 2D-NMR.
文摘A new isopimarane diterpene, isopimar-15-en-3 beta,8 beta,20-triol, was isolated from Nepeta prattii. Its structure was elucidated by spectral methods (EIMS, 1D and 2D NMR).
基金supported by National Science and Technology Support Program and Drug Safety Critical Technology (2006BAI14B00)funding subject-Common and Important Drug Safety Standards(2006BAI14B01)
文摘Euphorbia ebracteolata Hayata (E. ebracteolata) is a Chinese herbal medicine used for the treatment of tumor diseases. An ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS) based chemical profiling approach was established for the rapid separation and characterization on phloroglucinol derivatives and diterpenes in E. ebracteolata. Three phloroglucinol derivatives and nine diterpenes were identified by exact mass measurement and were further confirmed by Ms2 data. In addition, the chemical profiles of six compounds were acquired by reference standards. Furthermore, the fragmentation rules of phloroglucinol derivatives and diterpenes of E. ebracteolata were analyzed, and each chromatographic peak was classified.
基金financially supported by the National Foundation for Outstanding Young Scientists to X.J.Hao(No.39525025).
文摘Chemical investigation on the ethanol extract from the roots of Spiraea japonica var. acuta resulted in the isolation of two new diterpene alkaloids named spiratines A and B (1-2), respectively.
基金financially supported by the National Science and Technology Pillar Program during the Twelfth Five-year Plan Period(2012BAK17B00)the Doctoral Program of Higher Education,China(No.20114404120022)the Agricultural Science and Technology Planned Projects of Guangzhou,China(No.2012A020602038)
文摘In this study, ent-Kaurane-3β,16β,17-trioi (1) and ent-kaurane-2a,16β,17-triol (2), were isolated from the leaves ofRubus corchorifolius L. f. Their structures were elucidated based on extensive spectroscopic analysis. NMR experiments identified the two compounds and X-ray crystallographic analysis confirmed their crystal structures. The crystal of 1 belongs to monoclinic with space group P21 and a = 10.7641(3), b = 7.2789(3), c = 11.7862(4) A, β = 95.275(3)°, V = 919.55(6) A3, Z = 2, C20H34O4, Mr = 322.49 g/mol, Dc = 1.230 g/m3, F(000) = 376, 2 = 1.54178 A,μ = 0.661 mm-1, R = 0.0319, and wR = 0.0811 for 10889 observed reflections (I〉 2σ(I)). The crystal of 2 is classified as orthorhombic with space group P212121 and a = 10.7641(3), b = 12.72224(19), c = 21.3929(3) A, V= 1748.94(4) A3, Z= 4, C20H3404, Mr = 322.47, De = 1.225 Mg/m3 F(000) = 712, λ = 1.54184 A,μ = 0.625 mm-1, R = 0.0303 and wR = 0.0759 for 10470 observed reflections (1〉 2σ(I)). Meanwhile, the compound revealed low inhibitory activities toward HepG2, MCF-7, and SCG-7901 cells.
文摘Five new diterpenes were isolated from Euphorbia sieboldiana and identified as atis-16-en-13(S)-hydroxy-3,14-dione(Ⅰ),atis-16-en- 14-oxo-13(S),3 β-diol(Ⅱ),atisane-3-oxo-16α,17-diol(Ⅳ),atisane-3β ,16α,17-triol(Ⅴ)and atisane-3β-acetyl-16α,17-diol(Ⅵ)based on the chemical,spectral evidences and X-ray diffraction.
文摘s: Two new diterpene alkaloids named swinanine C and D were isolated from Delphinium swinanense var leptopogon (H. -M. ) W. T. Wang. Their structures were elucidated by means of spectral methods , principally 1H-NHR, DEPT and 13CNMR techniques.
文摘The antibacierial (staphylococcus aureus) activities of 23 diterpene quinones from the Salvia przewalskii Maxim, the artificial synthesis ones and that of the metabolism in pigs were examined in an effort to study the quantitative structure activity relationship with the Free-Wilson method. With the application of the multipiy regression, the Free-Wilson pattern was established by the dummy (indicator) variable of the structural fragments or substituents. The results showed: (1) The diketone moiety played the fundamental role in antibacterial activities, ortho-quinones was higher active than para-quinones. (2) The single bond existed between the C15 and the C16 at D-ring led to higher activity. (3) The saturation at the A-ring led to higher activity. (4) The components adjacent to nitrogen substituents at C15 led to higher activity. (5)The hydroxylation or dehydrogenation at A-ring led to less activity. The research results can provide the theoretical basis for developing a new antibacterial drug from the Salvia diterpene quinones.