[Objectives] To optimize the extraction process of total flavonoids from Sanguisorbae Radix and carbonized Sanguisorba root,compare quality of different batches of Sanguisorbae Radix,study the effects of processing on...[Objectives] To optimize the extraction process of total flavonoids from Sanguisorbae Radix and carbonized Sanguisorba root,compare quality of different batches of Sanguisorbae Radix,study the effects of processing on the content of flavonoids,and provide scientific basis for reasonable utilization of Sanguisorbae Radix. [Methods] Test samples were prepared by heating,refluxing,and extraction,the extraction process was optimized by orthogonal experiment design,color was developed by NaNO_2-Al( NO_3)3-NaOH,and total flavonoids were measured by UV method at the wavelength of 510 nm. [Results] The linear relationship of rutin was excellent in the concentration range of 0. 1248 mg/mL-0. 5712 mg/mL,R^2= 0. 9997; the average recovery was 99. 67% and the RSD was 0. 70%. The optimum extraction conditions were as follows: the volume fraction of ethanol was 50%,the extraction temperature was 90℃,the extraction time was 90 min,and the solid-to-liquid ratio was 1∶ 20( g/mL). [Conclusions] After optimization of the extraction process,the extraction rate of total flavonoids in samples of Sanguisorbae Radix was significantly increased; there was certain difference in the content of total flavonoids between different batches of Sanguisorbae Radix and processed products; the total flavonoids significantly declines in carbonized sanguisorba root,and the influence of processing on its curative effect was to be further studied.展开更多
Objective:To investigate whether Radix Sanguisorbae(RS,Diyu)could restore intestinal barrier function following sepsis using a cecal ligation and puncture(CLP)-induced septic rat model and lipopolysaccharide(LPS)-chal...Objective:To investigate whether Radix Sanguisorbae(RS,Diyu)could restore intestinal barrier function following sepsis using a cecal ligation and puncture(CLP)-induced septic rat model and lipopolysaccharide(LPS)-challenged IEC-6 cell model,respectively.Methods:Totally 224 rats were divided into4 groups including a control,sham,CLP and RS group according to a random number table.The rats in the control group were administrated with Ringer's lactate solution(30 mL/kg)with additional dopamine[10μg/(kg·min)]and given intramuscular injections of cefuroxime sodium(10 mg/kg)12 h following CLP.The rats in the RS group were administrated with RS(10 mg/kg)through tail vein 1 h before CLP and treated with RS(10 mg/kg)12 h following CLP.The rats in the sham group were only performed abdominal surgery without CLP.The rats in the CLP group were performed with CLP without any treatment.The other steps were same as control group.The effects of RS on intestinal barrier function,mesenteric microvessels barrier function,multi-organ function indicators,inflammatory response and 72 h survival window following sepsis were observed.In vitro,the effects of RS on LPS-challenged IEC-6 cell viability,the expressions of zona occludens-1(ZO-1)and ferroptosis index were evaluated by cell counting kit-8,immunofluorescence and Western blot analysis.Bioinformatic tools were applied to investigate the pharmacological network of RS in sepsis to predict the active compounds and potential protein targets and pathways.Results:The sepsis caused severe intestinal barrier dysfunction,multi-organ injury,lipid peroxidation accumulation,and ferroptosis in vivo.RS treatment significantly prolonged the survival time to 56 h and increased 72-h survival rate to 7/16(43.75%).RS also improved intestinal barrier function and relieved intestinal inflammation.Moreover,RS significantly decreased lipid peroxidation and inhibited ferroptosis(P<0.05 or P<0.01).Administration of RS significantly worked better than Ringer's solution used alone.Using network pharmacology prediction,we found that ferroptosis and hypoxia inducible factor-1α(HIF-1α)signaling pathways might be involved in RS effects on sepsis.Subsequent Western blot,ferrous iron measurements,and FerroOrange fluorescence of ferrous iron verified the network pharmacology predictions.Conclusion:RS improved the intestinal barrier function and alleviated intestinal injury by inhibiting ferroptosis,which was related in part to HIF-1α/heme oxygenase-1/Fe^(2+)axis.展开更多
Objective To study the chemical constituents in the effective fractions of charred Sanguisorbae Radix. Methods The compounds were isolated and purified by column chromatography and their structures were identified on ...Objective To study the chemical constituents in the effective fractions of charred Sanguisorbae Radix. Methods The compounds were isolated and purified by column chromatography and their structures were identified on the basis of physicochemical properties and spectral analysis. Results Five compounds were isolated and identified as 3β-hydroxy-28-norurs-17,19,21-trien (1), 3β-hydroxy-28-norurs-12,17-dien (2), 3β,19α-dihydroxyurs-13(18)- en-28-oic acid (3), 3β-[(α-L-arabin-opyranosyl) oxy]-28-norurs-12,17-dien (4), and pomolic acid (5). Conclusion Compounds 1, 3, and 4 are novel compounds belong to triterpenoids and triterpenoid saponins, named as sanguisorbigenins Z, Y1 , and Y2 , respectively.展开更多
Objective To explore the protective effects of tannins in Sanguisorba Radix （TSR） on myelosuppression mice induced by cyclophosphamide （CTX）. Methods TSR was ig given at the dose of 20 mg/kg for 10 d after ip admi...Objective To explore the protective effects of tannins in Sanguisorba Radix （TSR） on myelosuppression mice induced by cyclophosphamide （CTX）. Methods TSR was ig given at the dose of 20 mg/kg for 10 d after ip administration of CTX （200 mg/kg）. Results TSR could significantly increase the numbers of white blood ceils, red blood cells, and platelets of myelosuppression in mice. And it could accelerate bone marrow haemopoietic stem/progenitor cells （HSPCs） in myelosuppression mice and enhance cell proliferation by promoting cell cycles from G0/G1 phase to access into S and G2/M phases, then the reduced number of HSPCs induced by CTX was reversed. Moreover, TSR could increase the mRNA and protein expression levels of O（6）-methylguanine- DNA methyltransferase （MGMT） in HSPCs of myelosuppression mice. Concision TSR has a protective function against CTX-induced myelosuppression. The mechanism might be related to protecting hematopoietic stem cells of bone marrow, stimulating hematopoiesis recovery, as well as preventing the apoptosis of hematopoietic stem cells induced by CTX.展开更多
基金Supported by National Science and Technology Project of the Ministry of Science and Technology in the 13th Five-Year Plan Period(2015BAC05B02)Key Technology R&D Program of Sichuan Province,China(2015SZ0034)Innovating Research Program of Postgraduates of Southwest Minzu University in2016(CX2016SZ038)
文摘[Objectives] To optimize the extraction process of total flavonoids from Sanguisorbae Radix and carbonized Sanguisorba root,compare quality of different batches of Sanguisorbae Radix,study the effects of processing on the content of flavonoids,and provide scientific basis for reasonable utilization of Sanguisorbae Radix. [Methods] Test samples were prepared by heating,refluxing,and extraction,the extraction process was optimized by orthogonal experiment design,color was developed by NaNO_2-Al( NO_3)3-NaOH,and total flavonoids were measured by UV method at the wavelength of 510 nm. [Results] The linear relationship of rutin was excellent in the concentration range of 0. 1248 mg/mL-0. 5712 mg/mL,R^2= 0. 9997; the average recovery was 99. 67% and the RSD was 0. 70%. The optimum extraction conditions were as follows: the volume fraction of ethanol was 50%,the extraction temperature was 90℃,the extraction time was 90 min,and the solid-to-liquid ratio was 1∶ 20( g/mL). [Conclusions] After optimization of the extraction process,the extraction rate of total flavonoids in samples of Sanguisorbae Radix was significantly increased; there was certain difference in the content of total flavonoids between different batches of Sanguisorbae Radix and processed products; the total flavonoids significantly declines in carbonized sanguisorba root,and the influence of processing on its curative effect was to be further studied.
文摘Objective:To investigate whether Radix Sanguisorbae(RS,Diyu)could restore intestinal barrier function following sepsis using a cecal ligation and puncture(CLP)-induced septic rat model and lipopolysaccharide(LPS)-challenged IEC-6 cell model,respectively.Methods:Totally 224 rats were divided into4 groups including a control,sham,CLP and RS group according to a random number table.The rats in the control group were administrated with Ringer's lactate solution(30 mL/kg)with additional dopamine[10μg/(kg·min)]and given intramuscular injections of cefuroxime sodium(10 mg/kg)12 h following CLP.The rats in the RS group were administrated with RS(10 mg/kg)through tail vein 1 h before CLP and treated with RS(10 mg/kg)12 h following CLP.The rats in the sham group were only performed abdominal surgery without CLP.The rats in the CLP group were performed with CLP without any treatment.The other steps were same as control group.The effects of RS on intestinal barrier function,mesenteric microvessels barrier function,multi-organ function indicators,inflammatory response and 72 h survival window following sepsis were observed.In vitro,the effects of RS on LPS-challenged IEC-6 cell viability,the expressions of zona occludens-1(ZO-1)and ferroptosis index were evaluated by cell counting kit-8,immunofluorescence and Western blot analysis.Bioinformatic tools were applied to investigate the pharmacological network of RS in sepsis to predict the active compounds and potential protein targets and pathways.Results:The sepsis caused severe intestinal barrier dysfunction,multi-organ injury,lipid peroxidation accumulation,and ferroptosis in vivo.RS treatment significantly prolonged the survival time to 56 h and increased 72-h survival rate to 7/16(43.75%).RS also improved intestinal barrier function and relieved intestinal inflammation.Moreover,RS significantly decreased lipid peroxidation and inhibited ferroptosis(P<0.05 or P<0.01).Administration of RS significantly worked better than Ringer's solution used alone.Using network pharmacology prediction,we found that ferroptosis and hypoxia inducible factor-1α(HIF-1α)signaling pathways might be involved in RS effects on sepsis.Subsequent Western blot,ferrous iron measurements,and FerroOrange fluorescence of ferrous iron verified the network pharmacology predictions.Conclusion:RS improved the intestinal barrier function and alleviated intestinal injury by inhibiting ferroptosis,which was related in part to HIF-1α/heme oxygenase-1/Fe^(2+)axis.
基金National Natural Science Foundation of China-Study on the Mechanism of Diyutan Processing (30772786)
文摘Objective To study the chemical constituents in the effective fractions of charred Sanguisorbae Radix. Methods The compounds were isolated and purified by column chromatography and their structures were identified on the basis of physicochemical properties and spectral analysis. Results Five compounds were isolated and identified as 3β-hydroxy-28-norurs-17,19,21-trien (1), 3β-hydroxy-28-norurs-12,17-dien (2), 3β,19α-dihydroxyurs-13(18)- en-28-oic acid (3), 3β-[(α-L-arabin-opyranosyl) oxy]-28-norurs-12,17-dien (4), and pomolic acid (5). Conclusion Compounds 1, 3, and 4 are novel compounds belong to triterpenoids and triterpenoid saponins, named as sanguisorbigenins Z, Y1 , and Y2 , respectively.
基金Natural Science Fundation of China(No.81373976)Major and special project of National science and technology(No.2013ZX09103002-013)
文摘Objective To explore the protective effects of tannins in Sanguisorba Radix （TSR） on myelosuppression mice induced by cyclophosphamide （CTX）. Methods TSR was ig given at the dose of 20 mg/kg for 10 d after ip administration of CTX （200 mg/kg）. Results TSR could significantly increase the numbers of white blood ceils, red blood cells, and platelets of myelosuppression in mice. And it could accelerate bone marrow haemopoietic stem/progenitor cells （HSPCs） in myelosuppression mice and enhance cell proliferation by promoting cell cycles from G0/G1 phase to access into S and G2/M phases, then the reduced number of HSPCs induced by CTX was reversed. Moreover, TSR could increase the mRNA and protein expression levels of O（6）-methylguanine- DNA methyltransferase （MGMT） in HSPCs of myelosuppression mice. Concision TSR has a protective function against CTX-induced myelosuppression. The mechanism might be related to protecting hematopoietic stem cells of bone marrow, stimulating hematopoiesis recovery, as well as preventing the apoptosis of hematopoietic stem cells induced by CTX.
