Dynamic Non-Invasive Detection of NADH Based on Blood Flow-Mediated Skin Fluorescence (FMSF) Method

Nicotinamide adenine dinucleotide (NADH/NAD+) is involved in important biochemical reactions in human metabolism, including participation in energy production by mitochondria. The changes in fluorescence intensity as a function of time in response to blocking and releasing of blood flow in a forearm are used as a measure of oxygen transport with blood to the tissue, which directly correlates with the skin microcirculation status. In this paper, a non-invasive dynamic monitoring system based on blood flow-mediated skin fluorescence (FMSF) technology is developed to monitor the NADH fluorescence intensity of skin tissue during the process of blocking reactive hyperemia. Simultaneously, laser speckle contrast imaging (LSCI) and laser Doppler flowmetry (LDF) were used to observe blood flow, blood oxygen saturation (SOt2) and relative amount of hemoglobin (rHb) during the measurement process, which helped to explore NADH dynamics relevant physiological changes. A variety of parameters have been derived to describe NADH fluorescence curve based on the FMSF device. The experimental results are conducive to understanding the NADH measurement and the physiological processes related to it, which help FMSF to be a great avenue for in vivo physiological, clinical and pharmacological research on mitochondrial metabolism.

Measurements Of Cerebral Blood Flow in Postasphyxiated Newborns by Color Doppler Imaging（CDI）

Cerebral blood flow of 10 asphyxiated term newborns was continuously measured during the first 7 days of life and compared with that of 10 normal term infants by CDI. Frequency spectrum and blood flow variables in the anterior, middle and posterior cerebral arteries were studied. The results showed evidently lower systolic amplitude in patients than that in normal subjects. End diastolic amplitude was zero in part of vessels, and values of bloed flow variables were all lower in day 1 of the life as compared with the control groups. Frequency spectrum recovered to normal patterns in 9 survived infants in day 2. but blood flow variables recovered to normal by day 7. Values of resistance index (RI) rose to 1 in some vessels of moderate hypoxic ischemic encephalopathy (HIE) infants and stayed at 1 in the severe HIE infants. It is concluded that low CBF plays a key role in brain damage of post-asphyxiated newborns and RI may be an important parameter in the evaluation prognosis.

Mucosal blood flow measurements using laser Doppler perfusion monitoring

Perfusion of individual tissues is a basic physiological process that is necessary to sustain oxygenation and nutrition at a cellular level. Ischemia, or the insuff iciency of perfusion, is a common mechanism for tissue death or degeneration, and at a lower threshold, a mechanism for the generation of sensory signalling including pain. It is of considerable interest to study perfusion of pe- ripheral abdominal tissues in a variety of circumstances. Microvascular disease of the abdominal organs has been implicated in the pathogenesis of a variety of disorders, including peptic ulcer disease, inflammatory bowel disease and chest pain. The basic principle of laser Doppler perfusion monitoring (LDPM) is to analyze changes in the spectrum of light reflected from tissues as a response to a beam of monochromatic laser light emitted. It reflects the total local microcirculatory blood perfusion, including perfusion in capillaries, arterioles, venules and shunts. During the last 20-25 years, numerous studies have been performed in different parts of the gastroin-testinal (GI) tract using LDPM. In recent years we have developed a multi-modal catheter device which includes a laser Doppler probe, with the intent primarily to investigate patients suffering from functional chest pain of presumed oesophageal origin. Preliminary studies show the feasibility of incorporating LDPM into such catheters for performing physiological studies in the GI tract. LDPM has emerged as a research and clinical tool in preference to other methods; but, it is important to be aware of its limitations and account for them when reporting results.

Measurement of Liver Size and Intrahepatic Blood Flow of Red-eared Turtles by Color Doppler Ultrasound

In the study, a total of 20 red-eared turtles （ half male and half female） were selected to conduct the measurement of liver size and intrahepatic blood flow of red-eared turtles by color Doppler ultrasound. The results showed that the fight hepatic lobe could be scanned through the right carotid anterior acoustic win- dow, and the left hepatic lobe could be scanned through the left carotid anterior acoustic window, but the vision would be obstructed by the air in trachea. The liver could also be scanned through the left femoral anterior acoustic window and the fight femoral anterior acoustic window when filling of bladder was good. The correla- tion regression analysis suggested that estimated values of liver showed no linear relationship with weight, the longest back curve and the widest back curve. Through the study, the normal indicators for ultrasound examination of red-eared turtle liver were established, in order to provide a reference for examination of turtle liver.

Predicting Endometrium Receptivity with Parameters of Spiral Artery Blood Flow

In order To evaluate whether the parameters of spiral artery blood flow, as measured by transvaginal color Doppler, may be used to assess endometrium receptivity prior to embryo transfer (ET), a retrospective study of 94 infertile women who had undergone ART treatments with different outcomes (pregnant or nonpregnant) was done. Subendometrial blood flow was evaluated. The resistance index (RI), systolic/diastolic ratio (S/D) and pulsatility index (PI) were significantly lower in those who achieved pregnancy as compared with those who did not: 0.62±0.04 vs 0.68±0.04 (P<0.001), 2.66±0.33 vs 3.19±0.39 (P<0.01) and 1.15±0.17 vs 1.34±0.22 (P<0.05), respectively. Furthermore, when RI>0.72, PI>1.6, and S/D>3.6, no pregnancy occurred. These data suggest that the parameters of spiral artery blood flow could be used as a new assay in predicting endometrial receptivity before ET.

Changes of Gastric Mucosal Blood Flow in the Healing Process of Peptic Ulcers

Changes of gastro-duodenal mucosal blood flow (GDMBF) in the healingprocess of 82 patients with gastric ulcer,130 with duodenal ulcer and 9 with active ul-cer induced by gastric polypectomy were studied by endoscopic laser Doppler flowme-try.In 108 normal subjects,the mucosal blood flow at the angle and antrum of the les-ser curvature and on the anterior wall of the duodenal bulb was the smallest (P【0.01).In patients with gastric ulcer,gastric mucosal blood flow (GMBF)significantly decrea-Sed at the active stage and increased at the healing stage (P【0.01 ).GDMBF significan-tly increased (P【0.01) at the active stage and returned to normal at the healing stage ofduodenal ulcer.GDMBF was unchanged in the healing process of the active ulcer inducedby gastric polypectomy.The mucosal blood flow at ulcer centre and margins significan-tly decreased at the active stage and markedly increased at the healing stage as compa-red with that of surrounding mucosa.We conclude that GMBF plays an importantrole in the developing and healing process of peptic ulcers.

OCULAR BLOOD FLOW EVALUATION (POBF AND GDI) IN INJURED AND HEALTHY FELLOW EYES

Purpose: To assess if injured eyes develop ocular blood flow disturbances that may contribute to development of traumatic glaucoma. Materials and Methods: 25 patients hospitalized for eye injury and elevated IOP were evaluated 24 months after the trauma. All injured and fellow eyes underwent IOP measure, visual field (HFAII, Central 30/2SITA), pulsatile ocular blood flow analysis (POBF, OBF Labs UK), CDI examination (PSV, EDV, and RI) of Ophthalmic Artery, Central Retinal Artery, Short Posterior Ciliary Arteries. Results: IOP was significantly higher in injured eyes (15.1 vs 13.0±2.7mmHg) (P= 0,01), but only 2 eyes (8.8%) were under medical treatment POBF values were significantly lower in injured eyes: 11,25 μl/s in traumatised eyes and 15,40 μl/s in fellow eyes (P = 0.002). Resistivity Index (RI) of all investigated retrobulbar vessels was very significantly higher in injured eyes than in fellow eyes (P= 0.0001). There is no significant correlation between IOP and ocular blood flow disturbances.

Effect of swimming training on neural microcirculation in rats with sciatic nerve compression A study based on laser Doppler flowmetry

Microcirculation of the peripheral nerve is necessary for neural growth and regeneration. However, technical limitations have limited studies in this area. The few studies conducted have concerned active exercise effects on microcirculation of the peripheral nerve. Using an animal experiment, this study evaluated the effect of swimming training on microcirculation of injured peripheral nerve by laser Doppler flowmetry. The results showed that the blood vessel at the distal end of the peripheral nerve was the main blood supply for the nerve, and the internal blood supply for the nerve had strong compensatory ability. Swimming training promoted the functional recovery of rats with sciatic nerve injury and the regeneration of myelin sheath and blood vessels, but had no impact on neural blood flow.

Voluntary Thigh Muscle Strength with Resection Stump-Dependent Blood Flow and Vasodilation in an Amputated Lower Leg with Total Surface Bearing Prosthesis during Dynamic Knee Extensor: A Case Trial

Background: The magnitude of the hyperemic response due to repeated thigh stump exercise on incremental contraction intensity might be useful information in localized exercise tolerance for devising cardiovascular physical therapy for amputees. The effect of exercise on amputated leg blood flow (LBF) may potentially be altered due to voluntary muscle contractions after loss of the lower leg compared with the healthy leg. Case Presentation: A 57-year-old male patient with Burger disease attempted 3 min unilateral repeat/dynamic knee extensor exercise at a target muscle contraction frequency (1 s thigh muscle contraction and 1 s relaxation, 90 repetitions) with each leg <right transtibial amputated leg (AL) using a total surface-bearing prosthesis (TSB) and left non-AL> at six different contraction intensities (rubber resistance belt). Simultaneous measurement of blood velocity/flow (Doppler ultrasound) in the femoral artery, blood pressure, leg vascular conductance (LVC), and peak muscle strength (PMS) were performed during the 3 min exercise period. The maximum voluntary contraction by one-legged isometric knee muscle contraction was 14.7 kg in non-AL and 7.9 kg in the AL with prosthesis. The relative PMS was defined as “PMS/maximum voluntary contraction × 100 (%)”. Pre-exercise LBF was lower in the AL (200 ± 25 ml/min) than the non-AL (275 ± 74 ml/min). Both the non-AL and AL showed good positive linear relationships between absolute-/relative-PMS and LBF or LVC during 30 s at steady-state before the end of the exercise period. Furthermore, there was also similarity seen in the increase rate in LBF and/or LVC for the incremental relative PMS compared with the absolute PMS. Conclusion: In this case, the muscle strength depended on blood flow increase/vasodilation was seen in this “AL” using a TSB prosthesis for repeated dynamic knee extensor exercise. The present amputee’s limb muscle strengthening with the resection stump closely related to the degree of hyperemia in the amputated limb.

Gastric Mucosal Blood Flow and Acid Secretion in Rats

The relationship between gastric mucosal blood flow (GMBF) and gastric acid secretionwere studied in rats by using secretory stimulant (pentagastrin)and inhibitor(cimetidine). GMBFwas measured by Laser Doppler flowmetry (LDF) and gastric mucosal pH determined by microglasspH electrode. GMBF increased and gastric mucosal pH decreased significantly after intravenous injec-tion of 6μg/kg of pentagastrin; nevertheless GMBF decreased and gastric mucosal pH increasedmarkedly after intravenous administration of 100mg/kg of cimetidine. This indicates that pentagastrincan increase GMBF and gastric acid secretion, and cimetidine can decrease GMBF and gastric acidsecretion in rats, proving the close relationship between GMBF and gastric acid secretion in rats.

经阴道彩色多普勒超声在子宫内膜病变患者中的应用价值

目的分析经阴道彩色多普勒超声在子宫内膜病变患者中的应用价值。方法选取2021年2月至2022年4月南昌大学第四附属医院收治的88例子宫内膜病变患者作为研究对象,所有患者均接受经阴道彩色多普勒超声及病理学检查,按照病理学检查结果将患者分为息肉组与癌变组,每组44例。比较两组二维超声子宫内膜情况、病灶血流动力学情况、经阴道彩色多普勒超声血流相关参数;以病理学检查结果为金标准,分析经阴道彩色多普勒超声对子宫内膜癌变的诊断效能。结果癌变组子宫内膜厚度厚于息肉组,内膜-肌层分界清晰、病灶回声均匀占比均低于息肉组,差异有统计学意义(P<0.05);癌变组血流丰富占比高于息肉组,差异有统计学意义(P<0.05);癌变组血流阻力指数(RI)、搏动指数(PI)均低于息肉组,时间平均峰值流速(TAP)慢于息肉组,差异有统计学意义(P<0.05);经阴道彩色多普勒超声对子宫内膜癌变的诊断灵敏度为86.36%,特异度为84.09%,准确度为85.23%;诊断息肉的灵敏度为84.09%,特异度为86.36%,准确度为85.23%。结论经阴道彩色多普勒超声对子宫内膜病变的诊断准确率较高,能通过血流动力学、RI、PI、TAP及影像学表现鉴别息肉或癌变。

二维斑点追踪技术在评估冠心病患者左心室收缩与舒张功能中的应用价值

目的探讨二维斑点追踪技术在评估老年冠心病患者左心室收缩与舒张功能中的应用价值。方法对60例老年冠心病患者(研究组)和60名健康老年人(对照组),应用彩色多普勒超声诊断仪进行左心室功能检查。比较两组血压、生化指标(三酰甘油、高密度脂蛋白胆固醇、低密度脂蛋白胆固醇、总胆固醇、糖化血红蛋白)及常规超声参数、二维斑点超声应变参数、血流频谱测量超声参数。结果研究组受试者收缩压、舒张压及三酰甘油、高密度脂蛋白胆固醇、低密度脂蛋白胆固醇、总胆固醇、糖化血红蛋白水平均高于对照组(P<0.01)。两组受试者收缩末期和舒张末期的左室内径、室间隔壁厚度、左室前/后/下壁厚度比较,差异无统计学意义(P>0.05)。研究组受试者左室舒张充盈末流速峰值/左室收缩末流速峰值低于对照组(P<0.05);两组受试者左室射血分数、左室舒张充盈末流速峰值、左室收缩末流速峰值比较,差异无统计学意义(P>0.05)。研究组受试者舒张期和收缩期的左心室前壁、后壁、下壁、室间隔的纵向、径向、周向峰值绝对值均低于对照组(P<0.01)。结论二维斑点追踪技术的抗干扰能力较强,二维斑点超声应变参数能有效反映冠心病患者左室收缩、舒张情况,具有较高的应用价值。

床旁经颅多普勒超声在神经重症脑血管疾病患者中的监测价值

目的分析神经重症脑血管疾病患者监测中床旁经颅多普勒超声应用效果。方法筛选郑州人民医院2021年5月至2023年5月86例重症脑血管疾病患者作为研究对象(高血压脑出血,术后35例,未手术者7例,脑动脉瘤破裂出血,术后32例,未手术者5例,其他脑血管疾病术后4例,未手术者3例),均采用经颅多普勒超声(TCD)监测,包含经枕窗探测基底动脉(BA)、经颞窗探测双侧大脑中动脉(MCA),另监测收缩期峰值血流动力学改变、舒张末期峰值血流速度、平均峰值血流速度、血管阻力指数、血管搏动指数、血流频谱形态等了解血流动力学改变,了解血流动力学改变,依照监测结果分三组,脑血管痉挛组(n=26)、供血不足组(n=37)、血流增快组(n=29),对各组监测结果予以分析。结果患者均完成TCD监测,在不同病因中,脑动脉瘤破裂出血与高血压脑出血患者供血不足发生率最高,其次是血流增快、血管痉挛,三组发生率对比差异有统计学意义(P<0.05)。结论在神经重症脑血管疾病患者中,床旁TCD监测能为临床治疗及预后评价提供有效支持。

基于彩色多普勒超声分析胎儿脐血流对FGR的诊断价值

目的:探讨彩色多普勒超声胎儿脐血流参数对FGR的诊断价值。方法:回顾性选取2019年12月~2021年12月收治的100例FGR孕妇(研究组)及100例正常孕妇(对照组),均接受超声检测。分析胎儿超声脐血流参数与FGR的关系,Logistic回归分析FGR发生的影响因素。ROC曲线分析胎儿脐血流参数对FGR的诊断价值。结果:Logistic分析显示年龄、羊水过少、低蛋白血症、RI,PI,S/D均是FGR发病的影响因素。ROC分析显示S/D、RI、PI对FGR诊断价值高。结论:FGR胎儿彩色多普勒RI,PI,S/D异常升高,具有良好诊断价值。

子宫动脉血流多普勒超声联合血清PIGF、Vaspin、ESM-1诊断HDP价值

目的:探讨妊娠高血压疾病(HDP)患者子宫动脉血流多普勒超声、血清促血管生成因子(PIGF)、丝氨酸蛋白酶抑制剂(Vaspin)、内皮细胞特异分子-1(ESM-1)水平及其诊断价值。方法:回顾性收集2020年6月-2021年12月本院接诊的HDPL患者86例为病例组,产前检查健康孕妇75例为对照组,检测两组血清PIGF、Vaspin、ESM-1水平及子宫动脉血流参数,分析在诊断HDP价值。结果:病例组血清PIGF(271.56±45.56 pg/ml)低于对照组(330.15±50.35 pg/ml),Vaspin(0.46±0.04 ng/ml)、ESM-1(1.38±0.51μg/L)高于对照组(0.39±0.08 ng/ml、1.01±0.07μg/L),多普勒超声子宫动脉血流阻力指数(RI)(0.79±0.14)、搏动指数(PI)(0.83±0.21)、收缩期峰值流速与舒张末期血流速度比值(S/D)(2.26±0.74)均高于对照组(0.51±0.20、0.44±0.19、1.41±0.35),且病例组妊娠期高血压、轻度子痫前期、重度子痫前期患者上述各指标均有差异(均P<0.05)。受试者工作特征曲线分析,血清PIGF、Vaspin、ESM-1、RI、PI、S/D及各项联合,诊断HDP的曲线下面积分别为0.811、0.780、0.691、0.944、0.860、0.813、0.999,截断值分别为291.56 pg/ml、0.43 ng/ml、1.12μg/L、0.58、0.53、1.87,联合检测的特异度(96.5%)、准确度(93.4%)最高(均P<0.05)。结论:HDP患者血清PIGF、Vaspin、ESM-1、子宫动脉血流参数异常改变,且对HDP有较好诊断价值,本次研究也为靶向药物治疗HDP提供了新思路。

子宫动脉多普勒超声血流成像相关参数与妊娠期高血压产妇妊娠结局的关系

目的 探讨子宫动脉多普勒超声血流成像相关参数与妊娠期高血压产妇妊娠结局的关系。方法 选取80例妊娠期高血压产妇纳入病例组,80例健康产妇纳入健康组,比较两组的子宫动脉多普勒超声血流成像相关参数。比较病例组不同妊娠结局产妇的相关参数,分析不良妊娠结局的危险因素。结果 病例组的子宫动脉RI、 PI、 S/D均高于健康组(P <0.05)。病例组发生不良妊娠结局产妇的子宫动脉RI、 PI、 S/D均高于未发生产妇(P <0.05)。Logistic回归分析显示,子宫动脉RI、 PI、 S/D均为不良妊娠结局发生的独立危险因素(OR>1,P <0.05)。结论 妊娠期高血压产妇的子宫动脉RI、 PI、 S/D显著高于正常产妇,且子宫动脉RI、 PI、 S/D与不良妊娠结局有关,临床中可将多普勒超声检查用于病情诊断及不良妊娠结局风险预测。

阴茎动脉Doppler超声分析

目的研究健康成年男性阴茎背动脉(DA)、海绵体动脉(CA)PI、RI及S/D的正常值。方法将257名健康成年男性按年龄分为5组,分别为<30岁组65名,30～39岁组83名,40～49岁组61名,50～59岁组38名,60岁以上组10名。用Logidop!2型数字式Doppler超声血流仪检查双侧阴茎背动脉、双侧海绵体动脉PI、RI及S/D值。结果阴茎疲软状态下,阴茎背动脉、海绵体动脉PI、RI和S/D值个体左右比较无差异,各年龄组也无差异。建议正常参考值分别为:(1)LDA:PI1.43～3.43,RI0.72～0.92,S/D2.68～10.56。(2)RDA:PI1.47～3.47,RI0.73～0.93,S/D3.27～10.09。(3)LCA:PI1.49～3.21,RI0.74～0.90,S/D3.17～9.55。(4)RCA:PI1.93～3.27,RI0.72～0.90,S/D3.22～9.42。结论作为一种筛选手段,Doppler超声血流检测手段,有一定的应用价值。

高频彩超结合E-flow显像对早产儿早期脑血流灌注特点的观察

目的:探讨早产儿出生早期脑组织血流灌注特点。方法:应用高频超声结合E-flow血流显像技术,对体质量≥1 500 g早产儿(早产儿A组)35例、出生体质量<1 500 g(早产儿B组)33例、足月新生儿35例进行研究,在生后72小时内检测大脑中动脉(Middle cerebral artery,MAC)、豆纹动脉(Lenticulostriate artery,LSA)、豆纹动脉终末段(The terminal artery of lenticulostriate artery,LSAt)的血流参数,包括收缩期峰值流速(Vs)、舒张末期速度(Vd)、平均血流速度(Vm)、收缩期峰值流速与舒张末期速度的比值(S/D)、阻力指数(RI)。采用t检验比较各组间的差别。结果:早产儿A组MCA的Vs、Vd、Vm测值低于足月新生儿(P<0.05),且早产儿B组MCA的Vs、Vm低于早产儿A组(P<0.05);S/D、RI三组间比较无统计学差异(P>0.05)。早产儿A组的LSA、LSAt的血流参数Vs、Vd、Vm、S/D及RI均比足月儿组明显减低(P<0.01,P<0.05),且早产儿B组除S/D外,其余测值均低于早产儿A组(P<0.01)。结论:高频超声结合E-flow显像技术对脑实质内细小血管的血流参数进行检测,可更敏感地反映早产儿早期脑实质血流灌注的基础状态,为临床判断早产儿脑损伤时血流动力学变化提供依据。

子宫动脉血流多普勒超声检查联合血清胎盘生长因子、D-二聚体与孕中期子痫前期妊娠结局及新生儿预后的相关研究

目的 探讨子宫动脉血流多普勒超声检查联合血清胎盘生长因子(PIGF)和D-二聚体(D-D)与孕中期子痫前期孕妇妊娠结局及新生儿预后的相关性。方法 将2019年1月至2022年8月江阴市中医院(南京中医药大学江阴附属医院)就诊的89例孕中期先兆子痫孕妇纳入本次回顾性研究,设为先兆子痫组,另选择同时间段226例孕中期正常孕妇纳入对照组。比较各组孕20~26周子宫动脉血流参数[包括收缩期与舒张期流速比(S/D)、搏动指数(PI)和子宫动脉阻力指数(RI)],血清PIGF和D-D水平,Pearson相关性分析分析PIGF、D-D与S/D、PI和RI的相关性,比较不同母体结局及新生儿预后及子宫动脉血流参数、血清PIGF、D-D水平。结果 先兆子痫组S/D、PI和RI为3.35±0.26、2.21±0.12、0.84±0.21,均明显高于对照组(2.89±0.30、0.82±0.09、0.53±0.19),差异均有统计学意义(P<0.05)。先兆子痫组的血清PIGF为(13.41±0.59)μg/L,显著低于对照组[(42.35±2.21)μg/L],血清D-D为(598.05±79.51)μg/L,显著高于对照组[(264.19±45.96)μg/L],差异均有统计学意义(P<0.05)。Pearson相关性分析显示,PIGF与S/D、PI和RI呈负相关(r=-0.481、-0.509、0.489,P<0.05),而D-D与S/D、PI和RI呈正相关(r=0.422、0.447、0.438,P<0.05)。先兆子痫组新生儿出生孕周为(33.53±1.95)周,明显短于对照组[(38.97±2.27)周],出生体重及1 min Apgar评分分别为(2 324.10±413.77)g、(7.51±1.91)分,均明显低于对照组[(3 114.43±541.72)g、(9.50±1.83)分],差异均有统计学意义(P<0.05)。母体并发症发生率和新生儿不良预后发生率为68.54%(61/89)、47.19%(42/89)。母体并发症组的S/D、PI及RI、血清D-D水平均显著高于非母体并发症组,而血清PIGF水平显著低于非母体并发症组,差异均有统计学意义(P<0.05);新生儿预后不良组的的S/D、PI及RI、血清D-D水平显著高于预后良好组,而血清PIGF水平显著低于预后良好组,差异均有统计学意义(P<0.05)。结论 先兆子痫患者孕中期子宫动脉血流参数异常,血清PLGF、D-D表达异常,且先兆子痫患者新生儿预后差,孕中期子宫动脉血流参数、血清PLGF、D-D与先兆子痫患者母婴结局密切相关。

多普勒超声血流参数及血清脑钠肽、胰岛素生长因子-1水平与子痫前期病情及预后的相关性分析

目的:探索多普勒超声血流参数及血清脑钠肽(BNP)、胰岛素生长因子-1(IGF-1)水平与子痫前期(PE)病情及预后的相关性。方法:回顾性分析2019年2月至2021年5月万宁市人民医院收集的162例孕妇临床资料,将其中80例正常妊娠孕妇纳入健康对照组,82例PE孕妇纳入观察组。均进行血清BNP、IGF-1以及超声检测,根据受试者工作特征(ROC)曲线下面积(AUC),分析血流阻力指数(RI)、血管指数(VI)、肾脏血流指数(FI)、IGF-1及BNP诊断效能。采用相关性分析血流参数、血清指标与预后相关性。结果:观察组BNP为(90.61±41.71)pg/ml高于健康对照组,差异有统计学意义(t=12.334,P<0.05),VI为(17.35±4.86)%、FI为(34.29±5.55)、IGF-1为(110.35±33.36)g/L,均低于健康对照组,差异有统计学意义(t=7.483、10.163、15.147,P<0.05)。观察组中轻度PE为48例,重度PE为34例,重度PE的BNP为(121.46±37.75)pg/ml高于轻度PE,VI为(13.94±0.24)%、FI为(30.47±3.23)、IGF-1为(89.86±24.17)g/L均低于轻度PE,经Spearman法分析,病情严重程度和预后与VI、FI、IGF-1呈负相关性,与BNP呈正相关性。ROC曲线分析显示,RI、VI、FI、IGF-1、BNP及5项联合诊断重度PE的AUC值分别为0.500、0.888、0.873、0.772、0.862、0.983。观察组中预后良好67例,预后不良15例,预后不良BNP为(87.56±16.35)pg/ml,高于预后良好,差异有统计学意义(t=15.804,P<0.05),VI为(18.60±4.70)%、FI为(33.40±3.56)、IGF-1为(112.35±35.22)g/L,均低于预后良好,差异有统计学意义(t=2.574、5.362、8.750,P<0.05)。结论:血清BNP、IGF-1水平以及多普勒超声血流参数与子痫前期病情、预后存在一定相关性,可为后续治疗、预后提供客观依据。