Objective： A major problem in the chemotherapy of colon caner may be due to those cells that are in residence in the Go phase where they are less vulnerable to conventional therapy. To overcome this phenomenon, we at...Objective： A major problem in the chemotherapy of colon caner may be due to those cells that are in residence in the Go phase where they are less vulnerable to conventional therapy. To overcome this phenomenon, we attempted to recruit the reentry .of these cells into the cell cycle via a signaling pathway that manipulates tumor growth. Methods： Epidermal growth factor （EGF） was used to stimulate colon cancer caco-2 cells. FACS analysis and proliferating cell nuclear antigen （PCNA） staining were used to estimate the cell cycle transition and cell proliferation activated by EGF, and a MTT assay was used to evaluate the synergistic effect of EGF and chemotherapy. Results： The percentage of caco-2 ceils in the G0/G1 phase was significantly reduced by nearly 20% and the percentages in the S and G2/M phases were increased by EGF. The combined use of EGF and 5-fluorouracil （5-FU） enhanced the caco-2 cell chemosensitivity to 5-FU, reaching a maximum of an approximately threefold greater sensitivity than to 5-FU alone as judged by the 50% inhibiting concentration （IC50）. Conclusion： Our study demonstrated that stimulation by EGF enhanced the chemosensitivity of caco-2 cells to 5-FU, which may be a novel therapeutic protocol in colon cancer.展开更多
Objective This study aimed to review the available literatures on control of latent tuberculosis （TB） infection and propose a new control strategy to shorten the course of TB chemotherapy. Data sources The data used...Objective This study aimed to review the available literatures on control of latent tuberculosis （TB） infection and propose a new control strategy to shorten the course of TB chemotherapy. Data sources The data used in this review were mainly obtained from articles listed in PubMed. The search terms were ＂therapy （treatment） of tuberculosis; ＂therapy （treatment） of latent TB infection; and ＂vaccine of TB.＂ Study selection Articles regarding treatment and vaccine of TB were selected and reviewed. Results The most crucial reason causing the prolonged course of TB chemotherapy is the dormant state of Mycobacterium tuberculosis （M. tuberculosis）. Nevertheless, there are, to date, no effective drugs that can directly kill the dormant cells of M. tuberculosis in clinical therapy. In accordance with the growth cycle of dormant M. tuberculosis in the body, the methods for controlling dormant M. tuberculosis include direct killing with drugs, prevention of dormant M. tuberculosis resuscitation with vaccines, and resuscitating dormant M. tuberculosis with preparations or drugs and then thoroughly killing these resuscitated M. tuberculosis by using anti-TB therapy. Conclusions The comprehensive analysis of the above three methods suggests that the drugs directly killing dormant cells are in clinical trials, TMC207 is the most beneficial for controlling TB. Because the side effect of vaccines is less and their action period is long, prevention of dormant cells resuscitation with vaccines is promising. The last control method makes it probable that when a huge number of active cells of M. tuberculosis have been killed and eradicated after 1-month short chemotherapy, only a strong short-term subsequent chemotherapy can completely kill and eradicate the remaining M. tuberculosis. This control strategy is expected to significantly shorten the course of TB chemotherapy and bring a new change and breakthrough in TB treatment.展开更多
基金supported by the Science and Technology Department of Hubei Province(JX2A08)
文摘Objective： A major problem in the chemotherapy of colon caner may be due to those cells that are in residence in the Go phase where they are less vulnerable to conventional therapy. To overcome this phenomenon, we attempted to recruit the reentry .of these cells into the cell cycle via a signaling pathway that manipulates tumor growth. Methods： Epidermal growth factor （EGF） was used to stimulate colon cancer caco-2 cells. FACS analysis and proliferating cell nuclear antigen （PCNA） staining were used to estimate the cell cycle transition and cell proliferation activated by EGF, and a MTT assay was used to evaluate the synergistic effect of EGF and chemotherapy. Results： The percentage of caco-2 ceils in the G0/G1 phase was significantly reduced by nearly 20% and the percentages in the S and G2/M phases were increased by EGF. The combined use of EGF and 5-fluorouracil （5-FU） enhanced the caco-2 cell chemosensitivity to 5-FU, reaching a maximum of an approximately threefold greater sensitivity than to 5-FU alone as judged by the 50% inhibiting concentration （IC50）. Conclusion： Our study demonstrated that stimulation by EGF enhanced the chemosensitivity of caco-2 cells to 5-FU, which may be a novel therapeutic protocol in colon cancer.
文摘Objective This study aimed to review the available literatures on control of latent tuberculosis （TB） infection and propose a new control strategy to shorten the course of TB chemotherapy. Data sources The data used in this review were mainly obtained from articles listed in PubMed. The search terms were ＂therapy （treatment） of tuberculosis; ＂therapy （treatment） of latent TB infection; and ＂vaccine of TB.＂ Study selection Articles regarding treatment and vaccine of TB were selected and reviewed. Results The most crucial reason causing the prolonged course of TB chemotherapy is the dormant state of Mycobacterium tuberculosis （M. tuberculosis）. Nevertheless, there are, to date, no effective drugs that can directly kill the dormant cells of M. tuberculosis in clinical therapy. In accordance with the growth cycle of dormant M. tuberculosis in the body, the methods for controlling dormant M. tuberculosis include direct killing with drugs, prevention of dormant M. tuberculosis resuscitation with vaccines, and resuscitating dormant M. tuberculosis with preparations or drugs and then thoroughly killing these resuscitated M. tuberculosis by using anti-TB therapy. Conclusions The comprehensive analysis of the above three methods suggests that the drugs directly killing dormant cells are in clinical trials, TMC207 is the most beneficial for controlling TB. Because the side effect of vaccines is less and their action period is long, prevention of dormant cells resuscitation with vaccines is promising. The last control method makes it probable that when a huge number of active cells of M. tuberculosis have been killed and eradicated after 1-month short chemotherapy, only a strong short-term subsequent chemotherapy can completely kill and eradicate the remaining M. tuberculosis. This control strategy is expected to significantly shorten the course of TB chemotherapy and bring a new change and breakthrough in TB treatment.
