Objective: To study the stereoselective glucuronidation of carvedilol (CARV) by three Chinese liver microsomes. Methods: The metabolites of CARV were identified by a hydrolysis reaction with β-glucuronidase and HPLC-...Objective: To study the stereoselective glucuronidation of carvedilol (CARV) by three Chinese liver microsomes. Methods: The metabolites of CARV were identified by a hydrolysis reaction with β-glucuronidase and HPLC-MS/MS. The enzyme kinetics for CARV enantiomers glucuronidation was determined by a reversed phase-high pressure liquid chromatogra-phy (RP-HPLC) assay using (S)-propafenone as internal standard after precolumn derivatization with 2,3,4,6-tetra-O-acetyl-β-D-glucopyranosylisothiocyanate. Results: Two CARV glucuronides were found in three Chinese liver microsomes incubated with CARV. The non-linear regression analysis showed that the values of Km and Vmax for (S)-CARV and (R)-CARV enantiomers were (118±44) μmol/L, (2 500±833) pmol/(min·mg protein) and (24±7) μmol/L, (953±399) pmol/(min·mg protein), respectively. Conclusion: These results suggested that there was a significant (P<0.05) stereoselective glucuronidation of CARV enantiomers in three Chinese liver microsomes, which might partly explain the enantioselective pharmacokinetics of CARV.展开更多
CHINA is home to porcelain. There are thousands of art forms related to porcelain in the country. Among these art froms, carved porcelain is a very unique kind.
The carved lacquer craft,a gem of traditional Chinese crafts,boasts a history of over 2,000 years and features craftsmanship of a superior order.This study attempts to investigate the typical tasks and learning result...The carved lacquer craft,a gem of traditional Chinese crafts,boasts a history of over 2,000 years and features craftsmanship of a superior order.This study attempts to investigate the typical tasks and learning result evaluation of carved lacquer apprentices at the novice stage by the method of qualitative research with 11 carved lacquer craft inheritors of a studio of a carved lacquer master.It is discovered that the design of carved lacquer draft sketches,the design of carved lacquer patterns,and the making and use of carved lacquer tools are typical tasks of novice apprentices,while the modeling and aesthetics of works,carving skills,and the level of interaction with the carved lacquer community are important criteria for evaluating the development of the skills of novice apprentices.展开更多
At present, the protection of the legend of Ding Lan Carved Wood is declining and fails to give full play to its function of building filial culture. Combined with the examination of the contemporary inheritance and p...At present, the protection of the legend of Ding Lan Carved Wood is declining and fails to give full play to its function of building filial culture. Combined with the examination of the contemporary inheritance and protection of the legend of Ding Lan Carved Wood, we make full use of the existing resources of the legend of Ding Lan Carved Wood in urban areas and unite the cities of Xuzhou, Xingping, and Jiaozuo to put forward practical and favorable suggestions for development and inheritance, and jointly promote the exploration of the development and inheritance path of the filial culture of the legend of Ding Lan Carved Wood.展开更多
Students of the Faculty of Architecture in Sarajevo,through the course Architecture of the Old Age,were able to get acquainted with“architecture carved in rock”,and only a very limited number of examples-the archi&#...Students of the Faculty of Architecture in Sarajevo,through the course Architecture of the Old Age,were able to get acquainted with“architecture carved in rock”,and only a very limited number of examples-the architecture of ancient Egypt,ancient Greek architecture in Asia Minor(Lycia),and the architecture of ancient Persia.Many examples of this architectural expression remained unknown to them:the architecture of ancient and medieval India,the architecture of ancient and medieval China,the architecture of the ancient Middle East(the area of today’s Israel,Jordan,Saudi Arabia),vernacular architecture around the world from different historical periods.As a teacher at the Faculty of Architecture in Sarajevo,the author,after switching to the education of architecture students in accordance with the“Bologna process”(2003),presented“architecture carved in the rocks”through a series of subjects,through all levels of education(undergraduate,master’s and doctoral studies),within the framework of processing narrower topics of architecture:constructive systems in architecture,bioclimatic architecture,architecture as an energy system,architecture in context.The complex process of creating architecture and explaining its appearance was elaborated by the author(1988)in his doctoral dissertation.The topic“Architecture Carved in Rocks”was elaborated according to this model,which on the one hand confirmed its applicability,and on the other hand enabled students to look at the highly dispersed appearance of architecture in an extremely systematic way,without losing sight of its basic elements:environment,man,boundaries and perspectives.The aim of this work is to show(and through examples in Bosnia and Herzegovina)that natural caves,with more or less“refining”,are still used today,and that“carving architecture into rocks”is an all-time and planetary phenomenon.展开更多
基金the National Natural Science Foundation of China (No. 30225047)the Science and Technology Foundation of Zhejiang Province, China (No. 2005C13026)
文摘Objective: To study the stereoselective glucuronidation of carvedilol (CARV) by three Chinese liver microsomes. Methods: The metabolites of CARV were identified by a hydrolysis reaction with β-glucuronidase and HPLC-MS/MS. The enzyme kinetics for CARV enantiomers glucuronidation was determined by a reversed phase-high pressure liquid chromatogra-phy (RP-HPLC) assay using (S)-propafenone as internal standard after precolumn derivatization with 2,3,4,6-tetra-O-acetyl-β-D-glucopyranosylisothiocyanate. Results: Two CARV glucuronides were found in three Chinese liver microsomes incubated with CARV. The non-linear regression analysis showed that the values of Km and Vmax for (S)-CARV and (R)-CARV enantiomers were (118±44) μmol/L, (2 500±833) pmol/(min·mg protein) and (24±7) μmol/L, (953±399) pmol/(min·mg protein), respectively. Conclusion: These results suggested that there was a significant (P<0.05) stereoselective glucuronidation of CARV enantiomers in three Chinese liver microsomes, which might partly explain the enantioselective pharmacokinetics of CARV.
文摘CHINA is home to porcelain. There are thousands of art forms related to porcelain in the country. Among these art froms, carved porcelain is a very unique kind.
文摘The carved lacquer craft,a gem of traditional Chinese crafts,boasts a history of over 2,000 years and features craftsmanship of a superior order.This study attempts to investigate the typical tasks and learning result evaluation of carved lacquer apprentices at the novice stage by the method of qualitative research with 11 carved lacquer craft inheritors of a studio of a carved lacquer master.It is discovered that the design of carved lacquer draft sketches,the design of carved lacquer patterns,and the making and use of carved lacquer tools are typical tasks of novice apprentices,while the modeling and aesthetics of works,carving skills,and the level of interaction with the carved lacquer community are important criteria for evaluating the development of the skills of novice apprentices.
文摘At present, the protection of the legend of Ding Lan Carved Wood is declining and fails to give full play to its function of building filial culture. Combined with the examination of the contemporary inheritance and protection of the legend of Ding Lan Carved Wood, we make full use of the existing resources of the legend of Ding Lan Carved Wood in urban areas and unite the cities of Xuzhou, Xingping, and Jiaozuo to put forward practical and favorable suggestions for development and inheritance, and jointly promote the exploration of the development and inheritance path of the filial culture of the legend of Ding Lan Carved Wood.
文摘Students of the Faculty of Architecture in Sarajevo,through the course Architecture of the Old Age,were able to get acquainted with“architecture carved in rock”,and only a very limited number of examples-the architecture of ancient Egypt,ancient Greek architecture in Asia Minor(Lycia),and the architecture of ancient Persia.Many examples of this architectural expression remained unknown to them:the architecture of ancient and medieval India,the architecture of ancient and medieval China,the architecture of the ancient Middle East(the area of today’s Israel,Jordan,Saudi Arabia),vernacular architecture around the world from different historical periods.As a teacher at the Faculty of Architecture in Sarajevo,the author,after switching to the education of architecture students in accordance with the“Bologna process”(2003),presented“architecture carved in the rocks”through a series of subjects,through all levels of education(undergraduate,master’s and doctoral studies),within the framework of processing narrower topics of architecture:constructive systems in architecture,bioclimatic architecture,architecture as an energy system,architecture in context.The complex process of creating architecture and explaining its appearance was elaborated by the author(1988)in his doctoral dissertation.The topic“Architecture Carved in Rocks”was elaborated according to this model,which on the one hand confirmed its applicability,and on the other hand enabled students to look at the highly dispersed appearance of architecture in an extremely systematic way,without losing sight of its basic elements:environment,man,boundaries and perspectives.The aim of this work is to show(and through examples in Bosnia and Herzegovina)that natural caves,with more or less“refining”,are still used today,and that“carving architecture into rocks”is an all-time and planetary phenomenon.
