MiR-183-5p promotes the progression of non-small cell lung cancer through targeted regulation of FOXO1

Objective To investigate miR-183-5p targeting to forkhead box protein O1(FOXO1)and its corresponding effect on the proliferation,migration,invasion,and epithelial-mesenchymal transition(EMT)of non-small cell lung cancer(NSCLC)cells.Methods NSCLC tissues and adjacent normal tissues from 60 patients with NSCLC adenocarcinoma were obtained via pathological biopsy or intraoperative resection.Several cell lines were cultured in vitro,including the human normal lung epithelial cell line BEAS-2B and human NSCLC cell lines A549,SPCA-1,PC-9,and 95-D.miR-183-5p and FOXO1 mRNA expression in tissues and cells were detected by qRT-PCR;the corresponding correlations in NSCLC tissues were analyzed using the Pearson test,and the relationship between miR-183-5p expression and clinicopathological parameters was analyzed.The miR-183-5p-mediated regulation of FOXO1 was verified by bioinformatics prediction alongside double luciferase,RNA-binding protein immunoprecipitation(RIP)assay,and pull-down experiments.A549 cells were divided into control,anti-miR-NC,anti-miR-183-5p,miR-NC,miR-183-5p,miR-183-5p+pcDNA3.1,and miR-183-5p+pcDNA3.1-FOXO1 groups.Cell proliferation,invasion,migration,apoptosis,and cell cycle distribution were detected using an MTT assay,clone formation assay,Transwell assay,scratch test,and flow cytometry,respectively.The expression of EMT-related proteins in the cells was analyzed by western blotting.The effect of miR-185-3p silencing on the development of transplanted tumors was detected by analyzing tumor formation in nude mice.Results miR-183-5p expression was significantly higher in NSCLC tissues and cells than in adjacent normal tissues,whereas FOXO1 mRNA expression was significantly down-regulated.There was a significant negative correlation between miR-183-5p and FOXO1 mRNA in NSCLC tissues(P<0.05).Additionally,the expression of miR-183-5p was significantly correlated with tumor size,tumor differentiation,and tumor-node-metastasis stage in patients with NSCLC(P<0.05).miR-183-5p targeted and inhibited FOXO1 expression.Compared to the anti-miR-NC group,the cell proliferation,scratch healing rate,N-cadherin and vimentin protein expression,and the proportion of S phase cells were significantly lower in the anti-miR-183-5p group,whereas the protein expression of E-cadherin andα-catenin and the proportion of G0/G1 phase cells were significantly higher;additionally,the frequency of colony formation and invasion were significantly lower in the anti-miR-183-5p group(P<0.05).Compared to the miR-NC group,the cell proliferation,scratch healing rate,N-cadherin and vimentin protein expression,and the proportion of S phase cells in the miR-183-5p group were significantly higher,whereas the E-cadherin andα-catenin protein expression and the proportion of G0/G1 phase cells were significantly lower;furthermore,the frequency of colony formation and invasion were significantly higher in the miR-183-5p group(P<0.05).Compared with the miR-183-5p+pcDNA3.1 group,the OD value,scratch healing rate,N-cadherin and vimentin protein expression,and the proportion of S phase cells were significantly lower in the miR-183-5p+pcDNA3.1-FOXO1 group,whereas E-cadherin andα-catenin protein expression and the proportion of G0/G1 phase cells were significantly higher;additionally,the frequency of colony formation and invasion was significantly lower in the miR-183-5p+pcDNA3.1-FOXO1 group(P<0.05).Overall,silencing miR-185-3p inhibited the growth of transplanted tumors and promoted FOXO1 expression.Conclusion Overexpression of miR-183-5p can inhibit apoptosis and promote the proliferation,migration,invasion,and EMT,of NSCLC cells by down-regulating FOXO1 expression.

Individual Differences in Stress Responsiveness of the Hypothalamic-Pituitary-Adrenal Axis and Its Vasopressinergic Regulation in Old Monkeys

Stress adaptation is fundamental for health, and the hypothalamic-pituitary-adrenal axis (HPA) is one of its main mechanisms. Considerable data indicate that arginine vasopressin (AVP) related disturbances of stress adaptation can occur with aging. However, most studies of such kind have been performed on rodents, give contradictory results and fail to consider individual characteristics of the animals. The purpose of this study was to investigate individual HPA responsiveness to acute stress and its vasopressinergic regulation in old female rhesus monkeys that differ in their behavioral responses to stress. Animals with depression-like or anxiety-like behavior (DAB) responded with higher plasma levels of ACTH and AVP, lower levels of corticosteroids and higher cortisol/DHEAS molar ratios to restraint stress and to insulin-induced hypoglycemia compared with animals with healthy adaptive behavior. AVP and ACTH dynamics were closely correlated in most animals. AVP treatment produced differences in HPA responses similar to those produced by the stressors. The ACTH response to hypoglycemic stress in the DAB animal with highest HPA responsiveness was dramatically reduced by prior administration of a V1b receptor antagonist. These results suggest that the dysfunctions of HPA observed in old animals with DAB are caused by increased tone of the vasopressinergic system in regulation of HPA stress reactivity.

Physiological roles of mitogen-activated-protein-kinase-activated p38-regulated/activated protein kinase

Mitogen-activated protein kinases(MAPKs)are a family of proteins that constitute signaling pathways involved in processes that control gene expression,cell division, cell survival,apoptosis,metabolism,differentiation and motility.The MAPK pathways can be divided into conventional and atypical MAPK pathways.The first group converts a signal into a cellular response through a relay of three consecutive phosphorylation events exerted by MAPK kinase kinases,MAPK kinase,and MAPK.Atypical MAPK pathways are not organized into this three-tiered cascade.MAPK that belongs to both conventional and atypical MAPK pathways can phosphorylate both non-protein kinase substrates and other protein kinases.The latter are referred to as MAPK-activated protein kinases.This review focuses on one such MAPK-activated protein kinase,MAPK-activated protein kinase 5(MK5)or p38-regulated/activated protein kinase(PRAK).This protein is highly conserved throughout the animal kingdom and seems to be the target of both conventional and atypical MAPK pathways.Recent findings on the regulation of the activity and subcellular localization,bona fide interaction partners and physiological roles of MK5/PRAK are discussed.

Long noncoding RNA negative regulator of antiviral response contributes to pancreatic ductal adenocarcinoma progression via targeting miR-299-3p

BACKGROUND Pancreatic ductal cancer(PDAC)has high malignancy and poor prognosis.Long noncoding RNAs(lncRNAs)are associated with high levels of malignancy,including PDAC.However,the biological and clinical significance of negative regulator of antiviral response(NRAV)in PDAC is unclear.AIM To study the regulatory role of lncRNA NRAV in PDAC.METHODS GEPIA analyzed lncRNA NRAV and miRNA(miR-299-3p)expression levels in PDAC tissues and measured them in PDAC cells by quantitative measurements in real time.The specific role of NRAV and miR-299-3p in cell proliferation and transfer potential was evaluated by cell formation analysis,Cell Counting Kit-8 and Transwell analysis.The relationship between NRAV and miR-299-3p was studied by predictive bioinformatics,RNA immunoassay,and fluorescence enzyme analysis.In vivo experiments included transplantation of simulated tumor cells under naked mice.RESULTS The expression level of lncRNA NRAV was higher in both tumor tissues and cell lines of PDAC and was negatively associated with the clinical survival of PDAC patients.Functionally,overexpression of NRAV promoted cell proliferation and metastasis of PDAC cells,while knockdown of NRAV reversed these effects.Finally,NRAV was performed as a molecular sponge of miR-299-3p.Moreover,overexpression of miR-299-3p could reverse the promoting effects of NRAV on cell proliferation and metastasis of PDAC cells.CONCLUSION NRAV facilitates progression of PDAC as a molecular sponge of miR-299-3p and may be a potential molecular marker for diagnosis and treatment of PDAC.

Synthesis,Crystal Structure and Plant Growth Regulatory Activity of 1-(3-Amino-[1,2,4]triazol-1-yl)-3,3-dimethyl-butan-2-one

The title compound 1-（3-amino-[1,2,4]triazol-1-yl）-3,3-dimethyl-butan-2-one（3） was synthesized by Hofmann-alkylation reaction of 1-chloro-3,3-dimethyl-butan-2-one（1） and ~1H-[1,2,4]triazol-3-ylamine（2） with equal amount of K_2CO_3 as acid acceptor. The structure of compound 3 was characterized by ~1H NMR, 13 C NMR, HRMS and single-crystal X-ray diffraction. The compound crystallizes in the monoclinic system, space group P21/n with a = 5.7227（8）, b = 27.924（4）, c = 6.2282（7） ？, β = 101.892（11）°, V = 973.9（2） ？~3, Z = 4, T = 180.00（10） K, μ（MoKα） = 0.087 mm^（-1）, Dc = 1.243 g/cm^3, 3832 reflections measured（3.648≤θ≤26.022°）, 1916 unique reflections（Rint = 0.0359, Rsigma = 0.0572） used in all calculations. The final R = 0.0557（I 〉 2σ（I）） and w R = 0.1276（all data）. Bioassay showed that 3 displayed excellent activity as plant growth regulator with inducing lateral root formation and enhancing primary root elongation at 0.27 mmol/L（50 ppm） in soybeen（He Feng-50）. Good water solubility was found with 50 mg in 1 m L of water. Therefore, application of 3 in agriculture is more environmentally friendly due to cosolvent-free condition, and results in improved abiotic-stress tolerance by affecting the root growth. And furthermore, it can be used as a precursor to investigate the function of regulating plant root growth.

circ_0003204 regulates the osteogenic differentiation of human adipose-derived stem cells via miR-370-3p/HDAC4 axis

Human adipose-derived stem cells(hASCs)are a promising cell type for bone tissue regeneration.Circular RNAs(circRNAs)have been shown to play a critical role in regulating various cell differentiation and involve in mesenchymal stem cell osteogenesis.However,how circRNAs regulate hASCs in osteogenesis is still unclear.Herein,we found circ_0003204 was significantly downregulated during osteogenic differentiation of hASCs.Knockdown of circ_0003204 by si RNA or overexpression by lentivirus confirmed circ_0003204 could negatively regulate the osteogenic differentiation of hASCs.We performed dual-luciferase reporting assay and rescue experiments to verify circ_0003204 regulated osteogenic differentiation via sponging miR-370-3p.We predicted and confirmed that miR-370-3p had targets in the 3′-UTR of HDAC4 m RNA.The following rescue experiments indicated that circ_0003204 regulated the osteogenic differentiation of hASCs via miR-370-3p/HDAC4 axis.Subsequent in vivo experiments showed the silencing of circ_0003204 increased the bone formation and promoted the expression of osteogenic-related proteins in a mouse bone defect model,while overexpression of circ_0003204 inhibited bone defect repair.Our findings indicated that circ_0003204 might be a promising target to promote the efficacy of hASCs in repairing bone defects.

A Novel Cellular Autoaggregative Developmentally CRP Regulated Behaviour Generates Massively Chondrule-Like Formations over Surface of Old <i>Escherichia coli</i>K-12 Macrocolony Biofilms

How Escherichia coli bacteria develop a particular colonial, 3-D biofilm morphological pattern is still a poorly understood process. Recently, we reported a new E. coli K-12 morphotype exhibited by old macrocolonies described as volcano-like. The formative developmental process of this morphotype has been presented as a suitable experimental model for the study of 3D patterning in macrocolony biofilms. Here, we report the optical microscopy observations and genetic analysis that have unveiled the existence of a novel autoaggregative behaviour which generates massive lumpiness over the surface of the volcano-like macrocolonies. These lumpy formations are generated by the autoaggregation and strong interaction of tightly packed bacterial cells in structures with a chondrule-like appearance which give the colony’s surface its characteristic microscopic lumpy phenotype. Furthermore, they exhibit different levels of maturation from the edge to the center of the colony. Hence, its generation appears to follow a spatiotemporal program of development during the macrocolony’s morphogenesis. Interestingly, the agar’s hardness influences the morphology exhibited by these formations, with high agar concentration (1.5%, 15 g/L) suppressing its development. This new auto-aggregative E. coli’s behaviour does not require the activity of the biofilm master regulator CsgD, the adhesiveness of flagella, pili type 1, adhesin Ag43, β-1,6-N-acetyl-D-glucosamine polymer-PGA, cellulose or colanic acid, but it is under glucose repression and the control of cAMP receptor protein (CRP). The possible physiological role of these chondrule-like formations in the adaptability of the colony to different stressful environmental conditions is discussed.

长、短效促性腺激素释放激素激动剂在体外受精-胚胎移植中自身对照研究

目的比较促性腺激素释放激素激动剂(GnRH-a)减量长效长方案和短效长方案在体外受精-胚胎移植(IVF-ET)中的应用。方法 2011年1月至2012年5月在本院生殖中心进行重复长方案,第一周期长效长方案第二周期短效长方案者共64人,第一周期短效长方案第二周期长效长方案者共79人。回顾性分析143名患者的286个周期资料进行自身对照研究,按照方案分为长效长方案组(组A)和短效长方案组(组B)。结果患者平均年龄(32.0±4.0)岁,平均不育年限(4.7±3.2)年。两组启动剂量无显著差异,组B启动日卵泡刺激素(FSH)和雌二醇(E_2)水平均显著高于A组。组B促排卵时间明显缩短[(9.9±1.5)vs(11.8±1.6)天],促性腺激素(Gn)使用剂量明显减少[(2,093.2±615.7)Vs(2,610.8±770.2)IU]。人绒毛膜促性腺激素(HCG)日组B的E_2水平显著高于组A[(10,702.94±4,582.32)vs(9,621.04±4,330.15)pmol/L],孕酮(P)水平显著低于组A[(1.90±0.95)vs(2.54±1.27)nmol/L],差异均有统计学意义(P<0.05)。两组获卵数,正常受精率、移植胚胎数目及可用胚胎数目均无统计学差异。组A的卵胞浆内单精子注射(ICSI)周期成熟卵母细胞率显著高于组B[(82.2±18.7)vs(74.7±20.3)%]。每组均选取第二周期进行临床妊娠率和植入率的比较,组A和组B的临床妊娠率分别为42.3%和48.3%,胚胎植入率分别为22.3%和26.2%,差异均无统计学意义。两组自然流产率无统计学差异。结论单次注射的减量长效长方案可以获得与多次注射的短效长方案相似的妊娠结局。长效降调节对垂体的抑制程度更深,在促排卵过程中用药量更大,用药时间更长。

IVF-ET后黄体支持作用的评估

目的:研究IVF超排卵周期中,胚胎移植(ET)后是否需要黄体支持。方法:随机收集2001.12-2004.12时间本中心的237例23-39岁的不孕症妇女,其中60例自然排卵者为A组,177例采用GnRha长方案降调节超卵排,取卵后,因各种原因未进行移植者35例为B组;胚胎移植后4周B超确认宫内妊娠者随机分为C组(82例,采用短期黄体支持,自移植日起使用黄体酮80mg/d至移植后4周,即孕6周)和D组(60例,采用长期黄体支持,自移植日起使用黄体酮80mg/d至孕12周),比较A、B组妇女的黄体功能状况,C、D组的早期流产率。结果:B组月经来潮时间为取卵后11.5±0.5d,明显短于A组(排卵后13.9±1.4d),P<0.05,有统计学差异。B组黄体晚期(取卵后11d)孕酮水平下降明显,与黄体早期(取卵后2d)相比,比值为0.08±0.01,而A组黄体晚期的孕酮水平呈上升趋势,与黄体早期的比值为1.70±0.27,二组的比值差异非常显著(P<0.01)。C组早期流产率为7.3%,D组为6.7%,二者相比无统计学差异(P>0.05)。结论:①GnRha长方案降调节影响黄体功能。②移植后4周(孕6周)停用黄体酮并不增加流产率。

Ferroptosis mechanism and Alzheimer's disease

Regulated cell death is a genetically determined form of programmed cell death that commonly occurs during the development of living organisms.This process plays a crucial role in modulating homeostasis and is evolutionarily conserved across a diverse range of living organisms.Ferroptosis is a classic regulatory mode of cell death.Extensive studies of regulatory cell death in Alzheimer’s disease have yielded increasing evidence that fe rroptosis is closely related to the occurrence,development,and prognosis of Alzheimer’s disease.This review summarizes the molecular mechanisms of ferroptosis and recent research advances in the role of ferro ptosis in Alzheimer’s disease.Our findings are expected to serve as a theoretical and experimental foundation for clinical research and targeted therapy for Alzheimer’s disease.

不同剂量黄体酮在体外受精-胚胎移植中黄体支持的效果

目的:探讨不同剂量黄体支持对IVF-ET周期黄体期雌、孕激素水平及临床结局的影响。方法:回顾分析长方案超促排卵IVF-ET305周期,根据hCG日雌激素水平采取4种不同黄体支持方案;对照组53例,hCG日血清E2值≤8000pmol/L,胚胎移植日开始肌注黄体酮60mg/d到验孕日。实验组:A组43例,hCG日血清E2值≤4000pmol/L,在取卵第2天开始肌注黄体酮20mg/d联合2000IU hCG q3d×4次到验孕日;B组115例,hCG日E24000pmol/L～8000pmol/L,取卵后第2天开始肌注40mg/d黄体酮到验孕日;C组94例,hCG日血清E2值≥8000pmol/L,取卵后第2天开始肌注60mg/d黄体酮到验孕日。结果:4组患者年龄、不孕年限、不孕因素、Gn用药天数、用药量、移植胚胎数均无明显差异(P>0.05);4组hCG日雌激素水平、获卵数、ET日和种植窗期雌激素水平均有显著差异(P<0.05),但4组ET日及种植窗期的E2/P值无显著差异;4组的种植率和妊娠率分别为27.68%、32.18%、32.54%、29.33%和41.51%、46.51%、50.43%、42.55%,无统计学差异。结论:不同剂量黄体支持有助于维持黄体期雌、孕激素平衡,有利于减少黄体酮剂量而不影响妊娠结局。

GnRHa对性早熟女童下丘脑-垂体-性腺轴的调控作用

目的:探讨促性腺激素释放激素类似物(GnRHa)对特发性中枢性性早熟(ICPP)女童下丘脑-垂体-性腺轴(HPG轴)的调控作用。方法:选取2014年4月至2015年4月孝感市中心医院收治的符合纳入及排除标准的性早熟女童患儿84例,随机分为参照组与研究组(各42例),参照组给予西医药物常规处理,研究组给予GnRHa进行治疗,观察并分析两组患儿治疗前与治疗24个月时血清雌二醇(E_2)、促卵泡生成素(FSH)、黄体生成素(LH)的分泌水平、第二性征的变化以及临床疗效,并记录其不良反应。结果:治疗24个月时两组ICPP患儿的血清E_2、LH和FSH分泌水平的基值与峰值均分别明显低于治疗前,差异具有统计学意义(P<0.05),且治疗24个月时研究组的E_2、LH和FSH分泌水平的基值与峰值明显低于参照组,差异具有统计学意义(P<0.05)。治疗24个月时,参照组与研究组患儿的第二性征均被明显抑制,研究组患儿的月经撤退、乳房变小以及乳房TannerⅠ期均明显高于参照组,差异具有统计学意义(P<0.05),且研究组的总有效率为88.10%,显著大于参照组,差异具有统计学意义(P<0.05)。治疗过程中,研究组不良反应发生率为4.76%,明显小于参照组,差异具有统计学意义(P<0.05)。结论:GnRHa可通过抑制HPG轴而降低性激素(E_2、LH、FSH)的分泌水平,能够有效调节生长发育,抑制第二性征,对ICPP女童患儿有较好的临床治疗效果,且不良反应较小。

超低剂量长效达菲林降调节对体外受精-胚胎移植结局的影响

目的:探讨超低剂量长效促性腺激素释放激素激动剂(GnRH-a,达菲林)降调节对黄体期长方案体外受精-胚胎移植(IVF-ET)结局的影响。方法:回顾性分析2010年1月至2011年10月在温州医学院附属第一医院生殖医学中心行长方案治疗的1 287例年龄≤38岁患者,共1 287个治疗周期。所有患者在黄体中期注射GnRH-a≤0.6 mg,降调2周后开始促排卵。结果:1 287个周期患者,达菲林剂量≤0.6 mg(平均0.53 mg,约1/7全量),使用GnRH-a后2周,黄体生成素(LH)为(2.16±0.95)IU/L,雌二醇(E2)为(83.03±37.98)pmol/L。促排所需促性腺激素(Gn)总量为(1 710.03±463.42)IU,Gn天数为(10.70±1.73)d。绒毛膜促性腺激素(hCG)注射日LH为(2.75±2.26)IU/L,孕酮(P)为(3.57±2.89)nmol/L,E2为(9 833.21±5 685.94)pmol/L。平均获卵数为(12.24±5.85)个,优胚率为62.85%。154个周期因各种原因未移植,1 133个移植周期中,血hCG阳性率为60.90%,临床妊娠率为54.64%。结论:对于年龄≤38岁,卵巢功能尚正常的患者在黄体期长方案IVF-ET周期中,使用1/7剂量长效GnRH-a降调节能有效地使垂体达到去敏状态,减少Gn用量,而且获得了比较理想的实验室和临床结局。

长效和短效达菲林对垂体降调节的作用及对体外受精-胚胎移植结局的影响

目的探讨体外受精-胚胎移植(IVF-ET)中促性腺激素释放激素激动剂(GnRH-a)垂体降调节的方式及合适剂量。方法将行IVF-ET的152例患者采用黄体期长方案进行控制性超排卵,并根据使用达菲林的剂型及剂量分为4组。A组38例,于黄体中期采用长效达菲林1.1 mg皮下注射1次;B组37例,于黄体中期采用长效达菲林1.25 mg皮下注射1次;C组37例,于黄体中期采用短效达菲林0.05 mg.d-1,每日皮下注射直至注射绒膜促性腺激素(HCG)注射日;D组40例,于黄体中期采用短效达菲林0.1 mg.d-1,月经第3-7天减至0.05 mg.d-1,每日皮下注射直至HCG注射日。结果B组的降调节时间最短、用药量最大、促性腺激素用药时间最长,与其他3组比较差异均有统计学意义(均P<0.05)。B组HCG注射日雌二醇(E2)水平高于A组(P<0.05);C组HCG注射日孕酮(P)水平低于B组(P<0.05)。4组周期获卵数、受精率、优质胚胎数、胚胎种植率及临床妊娠率比较差异均无统计学意义(均P>0.05)。结论长效达菲林1.1 mg.d-1或短效达菲林0.05 mg.d-1的应用均能达到控制性超排卵的垂体降调节作用,并能减少促性腺激素用量及用药时间,且不影响IVF-ET结局。

增加垂体降调节时间和GnRH-a剂量改善IVF/ICSI-ET妊娠率和活产率

目的探讨延长促性腺激素释放激素激动剂(GnRH-a)降调节时间和增加GnRH-a剂量对IVF/ICSI-ET妊娠率和活婴分娩率的影响。方法回顾性分析4 941例采用短效GnRH-a长方案降调节的IVF/ICSI-ET周期临床资料。根据GnRH-a使用剂量及降调节时间不同分为A1(GnRH-a 0.05mg/d,<20d)、A2(GnRH-a 0.05mg/d,>20d)、B1(GnRH-a0.1mg/d,<20d)和B2组(GnRH-a 0.1mg/d,>20d)。比较4组患者的促性腺激素(Gn)剂量、种植率、妊娠率和活婴分娩率。结果 B2组降调节天数和种植率、妊娠率和活婴分娩率显著高于其他3组(P<0.05),而Gn剂量显著少于其他3组(P<0.05)。结论在使用短效GnRH-a长方案垂体降调节中,通过延长Gn启动前GnRH-a降调节时间、增加GnRH-a使用剂量可以改善IVF-ET妊娠率和活婴分娩率,且未显著增加Gn使用剂量。

运动与下丘脑-垂体-性腺轴(之二:运动对下丘脑-垂体-性腺轴的影响)

有关运动性疲劳产生的的机理及对它如何进行有效预防和消除的研究,一直是令运动医学工作者瞩目的前沿课题。运动性疲劳时出现的机体变化较为复杂,涉及物质代谢、神经、内分泌、免疫等各个方面。其中,神经递质、HPG轴与机体的物质代谢、运动能力及运动后的恢复密切相关。为此,从睾酮的生理作用和调节、神经递质、细胞因子和睾丸间质细胞的调控、反馈调节和运动对下丘脑-垂体-性腺轴的影响等层面,较系统地探讨运动与下丘脑-垂体-性腺轴之间的关系,为加快机体恢复,提高运动能力,以及科学训练提供理论依据。

SGK-1在缺氧诱导小鼠垂体瘤细胞AtT-20增殖、凋亡中的表达及作用探讨

目的观察血清和糖皮质激素调节蛋白激酶1(SGK-1)在缺氧诱导小鼠垂体瘤细胞AtT-20增殖、凋亡中的表达,并探讨其在这一过程中的作用。方法将AtT-20细胞随机分为观察组和对照组,观察组分别加入50、100、200μmol/L CoCl_2(模拟缺氧),对照组不处理,采用MTT法检测24、48、72、96 h时细胞增殖的A570值;将200μmol/L CoCl_2加入AtT-20细胞,培养0、24、48、72、96 h时采用流式细胞术测算细胞凋亡率,分别采用半定量PCR法和Western blot法检测SGK-1 mRNA及蛋白。将AtT-20细胞随机分为4组,A、B组转染干扰质粒SGK-1 siRNA,C、D组转染对照质粒si Con,转染24 h后A、C组加入200μmol/L CoCl_2,B、D组不处理,分别采用MTT法和流式细胞术检测24、48、72、96 h时细胞增殖的A570值及细胞凋亡率。结果缺氧处理72 h后,观察组随着CoCl_2浓度增加及作用时间延长,AtT-20细胞增殖的A570值逐渐降低,与对照组比较差异有统计学意义(P均<0.05)。与0 h时比较,处理24、48 h时细胞凋亡率无明显变化,处理72、96 h时细胞凋亡率增加(P均<0.05);与0 h时比较,处理24、48时AtT-20细胞SGK-1 mRNA及蛋白表达量均增加(P均<0.05),72 h后其表达量无显著变化(P均>0.05)。A组缺氧后各时点细胞增殖的A570值均低于其余各组,C组缺氧后72、96 h细胞增殖的A570值高于A组而低于B、C组(P均<0.05)。A组缺氧后各时点细胞凋亡率均高于其余各组,C组缺氧后72 h细胞凋亡率低于A组而高于B、C组(P均<0.05)。结论缺氧可抑制AtT-20细胞增殖、促进其凋亡,该过程中SGK-1表达增加可保护细胞免受缺氧导致的损伤。

运动与下丘脑-垂体-性腺轴(之一:下丘脑-垂体-性腺轴的调控)

有关运动性疲劳产生的的机理及对它如何进行有效预防和消除的研究,一直是令运动医学工作者瞩目的前沿课题。运动性疲劳时出现的机体变化较为复杂,涉及物质代谢、神经、内分泌、免疫等各个方面。其中,神经递质、HPG轴与机体的物质代谢、运动能力及运动后的恢复密切相关。为此,从睾酮的生理作用和调节、神经递质、细胞因子和睾丸间质细胞的调控、反馈调节和运动对下丘脑-垂体-性腺轴的影响等层面,较系统地探讨运动与下丘脑-垂体-性腺轴之间的关系,为加快机体恢复,提高运动能力,以及科学训练提供理论依据。

两种促性腺激素释放激素类似物对IVF-ET临床效果的比较

目的 比较国产亮丙瑞林与进口达菲林在IVF-ET改良长方案促排卵中的降调节效果以及对妊娠结局的影响。方法 回顾性分析2014年1~12月在吉林大学第二医院生殖医学中心接受IVF/ICSI-ET助孕治疗、应用改良长方案控制性促排卵635个周期的临床资料。根据所用药物的不同分为亮丙瑞林组（256个周期）和达菲林组（379个周期）,比较两组患者的一般资料、生殖激素水平、Gn用量和Gn天数、获卵数、受精率、种植率和临床妊娠率等。结果 两组患者均达到降调标准,降调第20天生殖激素水平与HCG日生殖激素水平比较无显著性差异（P〉0.05）;两组的Gn用量[（36.54±10.58）vs.（32.56±8.34）支]、Gn天数[（10.32±1.69）vs.（10.57±1.64）d]及获卵数[（10.35±5.72）vs.（10.56±5.35）枚]、受精率（70.58%vs.68.30%）、卵裂率（95.44%vs.95.79%）、优质胚胎率（66.37%vs.60.49%）、种植率（21.75%vs.22.55%）、临床妊娠率（35.78%vs.37.84%）等比较无显著性差异（P〉0.05）。达菲林组的新鲜胚胎移植周期取消率（70.71%）显著高于亮丙瑞林组（14.84%）（P〈0.05）。结论 在改良长方案促排卵时,国产亮丙瑞林与进口达菲林的降调节效果以及妊娠结局较为相似,并且国产亮丙瑞林更有利于减轻患者的经济负担,不失为IVF-ET降调节的又一个理想选择。