Purpose: To discuss the relationship of Bovine Melanin-Associated Antigen (BMAA) to Sympathetic Ophthalmia (SO) and Vogt-Kayanagi-Harada (VKH) syndrome.Methods:BMAA was isolated and purified from bovine iris and cilia...Purpose: To discuss the relationship of Bovine Melanin-Associated Antigen (BMAA) to Sympathetic Ophthalmia (SO) and Vogt-Kayanagi-Harada (VKH) syndrome.Methods:BMAA was isolated and purified from bovine iris and ciliary body and MTT method was used to measure the peripheral lymphocyte reactions of SO and VKH syndrome patients to BMAA. 15 cases of SO were included in the SO group with 11 males and 4 females. The average age was 37.5, ranging from 23 to 56 years; 15 patients with VKH syndrome were included in the VHK group with 7 males and 8 females. Their average age was 37.2 years, ranging from 24 to 69 years; 20 normal individuals without ocular and systemic autoimmune diseases were included in the control group.These included 9 males and 11 females, aging from 14 to 28 with an average of 23.8 years.Results: The OD values of each group were as follows: SO controls 0.327+0.032; SO+ BMAA 0.490+0.758; SO+PHA 0.5310+0.918; VKH controls 0.328+0.503; VKH+BMAA 0.430 +0.530; VKH +PHA 0.328 +0.484;Normal controls 0.304 +0.267; Normal +BMAA 0.343+0.326; Normal+PHA 0.477+0.598. The average OD values of peripheral lymphocytes to BMAA stimulation in SO patients and normal controls were 0.490 and 0.343 respectively. The difference was statistically significant, P＜ 0.001; The average OD values of peripheral lymphocytes to BMAA stimulation in SO and VKH syndrome patients were 0.470 and 0.430 respectively. The difference was not statistically significant,P ＞ 0.05.Conclusions:BMAA effectively stimulate the proliferative reaction of peripheral lymphocytes in SO patients and BMAA may act as an antigen and contribute in the pathological process of SO.No difference in proliferative reaction of peripheral lymphocytes was found between SO and VKH syndrome patients after BMAA stimulations and BMAA may be involved in the development of both SO and VKH yndrome.展开更多
目的总结Vogt-K oyanagi -Harada(V K H )综合征患者的神经系统损害表现，探讨有诊断价值的辅助检查方法。方法对13 例患者的临床资料、脑脊液、影像学和电生理检查结果进行回顾性分析。结果11 例患者出现头痛，其中2 例为首发症状；10...目的总结Vogt-K oyanagi -Harada(V K H )综合征患者的神经系统损害表现，探讨有诊断价值的辅助检查方法。方法对13 例患者的临床资料、脑脊液、影像学和电生理检查结果进行回顾性分析。结果11 例患者出现头痛，其中2 例为首发症状；10 例脑脊液检查结果全部异常（100%）；除常规检查外，5 例行病毒系列检查中1 例EBV-Ig M 阳性；8 例寡克隆区带（OB）检查5 例阳性；5 例髓鞘碱性蛋白(M B P )检查，1 例增高；9 例免疫球蛋白(Ig G )检查，4例增高；IgG24h合成率升高1例头部MRI 示视神经异常信号；1例视觉诱发电位潜伏期延长。结论头痛为本病常见症状，但缺乏特异性；脑脊液、影像学检查尤其是免疫学和病毒学指标以及诱发电位检查是主要的诊断依据。展开更多
基金This research has been supported by Guangzhou Scientific Research Fund and Sun Yat-sen University Scientific Research Fund
文摘Purpose: To discuss the relationship of Bovine Melanin-Associated Antigen (BMAA) to Sympathetic Ophthalmia (SO) and Vogt-Kayanagi-Harada (VKH) syndrome.Methods:BMAA was isolated and purified from bovine iris and ciliary body and MTT method was used to measure the peripheral lymphocyte reactions of SO and VKH syndrome patients to BMAA. 15 cases of SO were included in the SO group with 11 males and 4 females. The average age was 37.5, ranging from 23 to 56 years; 15 patients with VKH syndrome were included in the VHK group with 7 males and 8 females. Their average age was 37.2 years, ranging from 24 to 69 years; 20 normal individuals without ocular and systemic autoimmune diseases were included in the control group.These included 9 males and 11 females, aging from 14 to 28 with an average of 23.8 years.Results: The OD values of each group were as follows: SO controls 0.327+0.032; SO+ BMAA 0.490+0.758; SO+PHA 0.5310+0.918; VKH controls 0.328+0.503; VKH+BMAA 0.430 +0.530; VKH +PHA 0.328 +0.484;Normal controls 0.304 +0.267; Normal +BMAA 0.343+0.326; Normal+PHA 0.477+0.598. The average OD values of peripheral lymphocytes to BMAA stimulation in SO patients and normal controls were 0.490 and 0.343 respectively. The difference was statistically significant, P＜ 0.001; The average OD values of peripheral lymphocytes to BMAA stimulation in SO and VKH syndrome patients were 0.470 and 0.430 respectively. The difference was not statistically significant,P ＞ 0.05.Conclusions:BMAA effectively stimulate the proliferative reaction of peripheral lymphocytes in SO patients and BMAA may act as an antigen and contribute in the pathological process of SO.No difference in proliferative reaction of peripheral lymphocytes was found between SO and VKH syndrome patients after BMAA stimulations and BMAA may be involved in the development of both SO and VKH yndrome.
文摘目的总结Vogt-K oyanagi -Harada(V K H )综合征患者的神经系统损害表现，探讨有诊断价值的辅助检查方法。方法对13 例患者的临床资料、脑脊液、影像学和电生理检查结果进行回顾性分析。结果11 例患者出现头痛，其中2 例为首发症状；10 例脑脊液检查结果全部异常（100%）；除常规检查外，5 例行病毒系列检查中1 例EBV-Ig M 阳性；8 例寡克隆区带（OB）检查5 例阳性；5 例髓鞘碱性蛋白(M B P )检查，1 例增高；9 例免疫球蛋白(Ig G )检查，4例增高；IgG24h合成率升高1例头部MRI 示视神经异常信号；1例视觉诱发电位潜伏期延长。结论头痛为本病常见症状，但缺乏特异性；脑脊液、影像学检查尤其是免疫学和病毒学指标以及诱发电位检查是主要的诊断依据。
