[ Objective] In order to make out differences between drug resistance spectrum types of different animal sources of Salmonella. [ Meth- otis] Selenite cystine broth, Salmonella- Shigella genus agar medium, CHROMagar S...[ Objective] In order to make out differences between drug resistance spectrum types of different animal sources of Salmonella. [ Meth- otis] Selenite cystine broth, Salmonella- Shigella genus agar medium, CHROMagar Salmonella chromogenic culture, Reveal Salmonella detection kit and molecular biology methods were used for isolation and identification of Salmonella from different animal feces samples. Antibacterialantimi- crobial susceptibility tests of 17 kinds of drugs such as enrofloxacin, apramycin and florfenicol were done on isolated strains with broth microdilution method, and test data was analysed with WHOnet 5.4 software. [ Results ] 699 feces samples from different animals were separated, and 53 strains of Salmonella were achieved with separation rate of 7.58%, among which those of yak, poultry, and pet sources were respectively 4.33%, 9.06% and 8.37%. [ Condusions] Separation rates and drug resistance spectrum types of different animal origin Salmonella varied greatly, among which separation rates and drug resistance spectrum types of yak source Salmonella were the lowest, there was little difference between separation rates of poultry and dog source Salmonella, however there were large differences on drug resistance spectrum types.展开更多
基金funded by 12th Five-Year Technology Support Program-key pastoral areas production of ecological life protection technology integration and demonstration topics-comprehensive prevention and control technology integration,innovation and application of cattle and sheep key epidemic disease(2012BAD13B06)Veterinary Medicine Discipline Program of Southwest University for Nationalities(2011XWD-S0906)
文摘[ Objective] In order to make out differences between drug resistance spectrum types of different animal sources of Salmonella. [ Meth- otis] Selenite cystine broth, Salmonella- Shigella genus agar medium, CHROMagar Salmonella chromogenic culture, Reveal Salmonella detection kit and molecular biology methods were used for isolation and identification of Salmonella from different animal feces samples. Antibacterialantimi- crobial susceptibility tests of 17 kinds of drugs such as enrofloxacin, apramycin and florfenicol were done on isolated strains with broth microdilution method, and test data was analysed with WHOnet 5.4 software. [ Results ] 699 feces samples from different animals were separated, and 53 strains of Salmonella were achieved with separation rate of 7.58%, among which those of yak, poultry, and pet sources were respectively 4.33%, 9.06% and 8.37%. [ Condusions] Separation rates and drug resistance spectrum types of different animal origin Salmonella varied greatly, among which separation rates and drug resistance spectrum types of yak source Salmonella were the lowest, there was little difference between separation rates of poultry and dog source Salmonella, however there were large differences on drug resistance spectrum types.
