[ Objective] This paper aimed to understand drug resistance of Escherichia coli isolated from pets in Guangzhou. I Method ] Broth microdilution method was used to determine the minimal inhibitory concentration （MIC）...[ Objective] This paper aimed to understand drug resistance of Escherichia coli isolated from pets in Guangzhou. I Method ] Broth microdilution method was used to determine the minimal inhibitory concentration （MIC） of ceftiofur, enrofloxacin and other 11 kinds of antibiotics for antimicrobial agent susceptibility testing of 120 strains of clinical E. coli and the experimental data were processed by WHONET 5.4 software. Based on the results of drug resistance pattern analysis, it helped to analyze and study the resistance mechanism. EResultl Clinical E. coli isolated from pets showed a different drug resistance to the 13 kinds of veterinary clinical antibiotics, and the different sources of E. coli also showed a different drug resistance pattern.[Cenclmion] The study provided a theoretical basis for the clinical use of drugs and druq resistance surveillance.展开更多
Since the successful preparation of the microplates and the medium for field application,the resistance degree and its geographical distribution of chloroquine-resistant Plasmodium falciparum,the fluctuation of the re...Since the successful preparation of the microplates and the medium for field application,the resistance degree and its geographical distribution of chloroquine-resistant Plasmodium falciparum,the fluctuation of the resistance degree of P.falciparum to chloroquine,and the sensitivity of the parasite to commonly used antimalarial drugs were investigated between 1980 and 2003 by the in vitro microtest and the in vivo four-week test recommended by the World Health Organization(WHO).The results indicated that chloroquine-resistant falciparum malaria was present in all eight provinces/autonomous regions endemic for falciparum malaria in China,and the resistance was high and widely distributed in the Hainan and Yunnan provinces.When the use of chloroquine was stopped or administered in a decreased quanity,the drug resistance gradually decreased.In Hainan and Yunnan,P.falciparum was still highly resistant to chloroquine,amodiaquine and piperaquine,and sensitive to pyronaridine and artemisinin derivatives,but the sensitivity was gradually reduced.Based on these results,principles and therapeutic regimens for antimalarial drug use in China were formulated,the use of the antimalarials which had already developed resistance was stopped or reduced,and recommendations to use artemisinin derivatives or compound pyronaridine to promote a rational use of antimalarials and strengthen malaria control were made.The results showed that malaria incidence had declined,and endemic areas of falciparum malaria have been gradually reducing since the mid-1980s.展开更多
Objective According to the guidelines of WHO/IUATLD proposed a global drug resistance surveillance of tuberculosis project,to set up a system for surveillance of drug resistance of pulmonary tuberculosis.to obtain the...Objective According to the guidelines of WHO/IUATLD proposed a global drug resistance surveillance of tuberculosis project,to set up a system for surveillance of drug resistance of pulmonary tuberculosis.to obtain the result on initianl and acquired drug resistance rate of pulmonary tuberculosis in Guangdong province of China.Methods 40 centres were radomly selected,39 cases of the smear positive of pulmonary tuberculosis were intook continuously in each centre.all samples were isolated and cultured.the identification and in each centre.all samples were isolated and cultured.the identification and susceptibility test were done for the strians of culture positive.Results The frequency of initial drug resistance was 18%,acquired drug resistance was 33.7%.Conclusion The result proved that drug resistance rate is higher(18%)in Guangdong province.National TB Control Program should be strengthen.展开更多
基金supported by the Special Fund Project for the Institute Science and Technology Development and Demostration Extention of Henan Provincial Academy of Argricultural Sciences(Research of Zoonotic Small Animal Diseases)the Project for the 2012 Annual"University Students'Innovation Training Program",Southwest University for Nationalities(201210656017)the Special Fund Project for the Central University Basic Scientific Research Fund,Southwest University for Nationalities(11NZYTD02)
文摘[ Objective] This paper aimed to understand drug resistance of Escherichia coli isolated from pets in Guangzhou. I Method ] Broth microdilution method was used to determine the minimal inhibitory concentration （MIC） of ceftiofur, enrofloxacin and other 11 kinds of antibiotics for antimicrobial agent susceptibility testing of 120 strains of clinical E. coli and the experimental data were processed by WHONET 5.4 software. Based on the results of drug resistance pattern analysis, it helped to analyze and study the resistance mechanism. EResultl Clinical E. coli isolated from pets showed a different drug resistance to the 13 kinds of veterinary clinical antibiotics, and the different sources of E. coli also showed a different drug resistance pattern.[Cenclmion] The study provided a theoretical basis for the clinical use of drugs and druq resistance surveillance.
文摘Since the successful preparation of the microplates and the medium for field application,the resistance degree and its geographical distribution of chloroquine-resistant Plasmodium falciparum,the fluctuation of the resistance degree of P.falciparum to chloroquine,and the sensitivity of the parasite to commonly used antimalarial drugs were investigated between 1980 and 2003 by the in vitro microtest and the in vivo four-week test recommended by the World Health Organization(WHO).The results indicated that chloroquine-resistant falciparum malaria was present in all eight provinces/autonomous regions endemic for falciparum malaria in China,and the resistance was high and widely distributed in the Hainan and Yunnan provinces.When the use of chloroquine was stopped or administered in a decreased quanity,the drug resistance gradually decreased.In Hainan and Yunnan,P.falciparum was still highly resistant to chloroquine,amodiaquine and piperaquine,and sensitive to pyronaridine and artemisinin derivatives,but the sensitivity was gradually reduced.Based on these results,principles and therapeutic regimens for antimalarial drug use in China were formulated,the use of the antimalarials which had already developed resistance was stopped or reduced,and recommendations to use artemisinin derivatives or compound pyronaridine to promote a rational use of antimalarials and strengthen malaria control were made.The results showed that malaria incidence had declined,and endemic areas of falciparum malaria have been gradually reducing since the mid-1980s.
文摘Objective According to the guidelines of WHO/IUATLD proposed a global drug resistance surveillance of tuberculosis project,to set up a system for surveillance of drug resistance of pulmonary tuberculosis.to obtain the result on initianl and acquired drug resistance rate of pulmonary tuberculosis in Guangdong province of China.Methods 40 centres were radomly selected,39 cases of the smear positive of pulmonary tuberculosis were intook continuously in each centre.all samples were isolated and cultured.the identification and in each centre.all samples were isolated and cultured.the identification and susceptibility test were done for the strians of culture positive.Results The frequency of initial drug resistance was 18%,acquired drug resistance was 33.7%.Conclusion The result proved that drug resistance rate is higher(18%)in Guangdong province.National TB Control Program should be strengthen.
