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Isolation and Identification of Saccharomycetes in Wine Grape Region of Chateau Changyu Moser XV
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作者 王志恒 刘雅琴 +1 位作者 冯翠娥 王冲 《Agricultural Science & Technology》 CAS 2016年第12期2689-2691,2700,共4页
Objective] This study was conducted to further explore the diversity of Saccharomyces cerevisiae_from Chateau Changyu Moser XV and realize better de-velopment and utilization of Saccharomyces cerevisiae resources. [Me... Objective] This study was conducted to further explore the diversity of Saccharomyces cerevisiae_from Chateau Changyu Moser XV and realize better de-velopment and utilization of Saccharomyces cerevisiae resources. [Method] ln this study, the wine grape regions of Chateau Changyu Moser XV were taken as the research object. The Saccharomycetes_in the soil was isolated, screened and ob-served in the natural fermentation process of grape berry epidermis and the fruit. The 32 Saccharomycetes strains were preliminarily classified based on WL nutrient agar, and 26S rDNA D1/D2 sequence analysis was conducted. [Result] Total y, 4 kinds of Saccharomycetes were identified in this study, including Pichia kluyveri, Hanseniaspora uvarum, Saccharomyces cerevisiae_and Cryptococcus magnus. [Con-clusion] The main species of Saccharomycetes in the wine grape region of Chateau Changyu Moser XV were preliminarily determined, which provides theoretical basis and research basis for the brewing of wine with special characteristics. 展开更多
关键词 Chateau Changyu Moser XV wine grape lsolation and screening Molecular identification
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The Effect of Vineyard Cover Crop on Main Monomeric Phenols of Grape Berry and Wine in Vitis viniferal L. cv. Cabernet Sauvignon 被引量:13
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作者 XI Zhu-mei ZHANG Zhen-wen CHENG Yu-feng LI Hua 《Agricultural Sciences in China》 CAS CSCD 2010年第3期440-448,共9页
This study was conducted to determine the effect of cover crop inter-row in vineyard on main mono-phenol content of grape berry and wine. Three such cover crops, two perennial legumes (white clover and alfalfa) and ... This study was conducted to determine the effect of cover crop inter-row in vineyard on main mono-phenol content of grape berry and wine. Three such cover crops, two perennial legumes (white clover and alfalfa) and a perennial gramineous grass (tall fescue) were sown in vineyard. The main phenolic compounds of mature grape berry and wines vinified under the same conditions were extracted with ethyl acetate and diethyl ether and analyzed by high- performance liquid chromatography (HPLC) by comparing to soil tillage. A total of ten phenolic compounds were identified and quantified in the different grape berry and wines, including nonflavonoids (hydroxybenzoic and hydroxycinnamic acids) and flavonoids (flavanols and flavonols). The concentration of flavonoid compounds (409.43 to 538.63 mg kg^-1 and 56.16 to 81.30 mg L^-1) was higher than nonflavonoids (76.91 to 98.85 mg kg^-1 and 30.65 to 41.22 mg L^-1) for Cabernet Sauvignon grape and wine under different treatments, respectively. In the flavonoid phenolics, Catechin was the most abundant in the different grapes and wines, accounting for 74.94 to 79.70% and 48.60 to 50.62% of total nonanthocyanin phenolics quantified, respectively. Compared to soil tillage, the sward treatments showed a higher content of main mono-phenol and total nonanthocyanin phenolics in grapes and wines. There were significant differences between two cover crop treatments (tall fescue and white clover) and soil tillage for the content of benzoic acid, salicylic acid, caffeic acid, catechin, and total phenolics in the grape berry (P 〈 0.05 or P〈0.01). The wine from tall fescue cover crop had the highest gallic acid, caffeic acid and catechin. Cover crop system increased the total nonanthocyanin phenolics of grapes and wines in order of the four treatments: tall fescue, white clover, alfalfa, and soil tillage (control). Cover crop in vineyard increased total phenols of grape berry and wine, and thus improved the quality of wine evidently. 展开更多
关键词 VINEYARD cover crops inter-row Cabernet Sauvignon grape berry wine monomeric phenols
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Effects of the severity and timing of basal leaf removal on the amino acids profiles of Sauvignon Blanc grapes and wines 被引量:4
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作者 YUE Xiao-feng JU Yan-lun +3 位作者 TANG Zi-zhu ZHAO Ya-meng JIAO Xu-liang ZHANG Zhen-wen 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2019年第9期2052-2062,共11页
The effects of the severity and timing of leaf removal(LR)on the amino acids of Sauvignon Blanc grapes and wines were studied during the 2017 growing season.High-performance liquid chromatography(HPLC)was used to anal... The effects of the severity and timing of leaf removal(LR)on the amino acids of Sauvignon Blanc grapes and wines were studied during the 2017 growing season.High-performance liquid chromatography(HPLC)was used to analyze the amino acids profiles of grape berries and wines.The basal leaves were removed at three time points(40,56 and 72 days after flowering,named LR40,LR56 and LR72,respectively)at two severity levels(one at which the first,third,and fifth basal leaves of each shoot were removed(50%level);and another at which the first six basal leaves were removed(100%level)).The results showed that leaf removal had little impact on total soluble solids(°Brix),titratable acidity,pH or berry weight.The LR72-50%treated grapes had higher berry weight,titratable acidity and°Brix than those of the other treatments.The highest concentrations of total amino acids and of total amino acids except proline were detected in LR72-50%treated grapes(2 952.58 and 2 764.36 mg L^-1,respectively);the lowest were detected in LR72-100%treated grapes(2 172.82 and 2 038.71 mg L^-1,respectively).LR72-50%treatment significantly promoted the synthesis of aspartic acid,serine,arginine,alanine,aminobutyric acid and proline at both severity levels for grapes,the concentrations of all of these amino acids were increased relative to the control concentrations.The LR72-50%,LR40-100%and LR72-100%treated wines had higher total amino acids concentrations and higher concentrations of some individual amino acids,such as arginine,alanine and serine,than did the control wines.Of all the amino acids studied,glycine,tyrosine,cysteine,methionine and lysine were not significantly influenced by the timing or severity basal defoliation in grapes and wines.The present study reveals the effects of the timing and severity of leaf removal on the amino acids profiles of grapes and wines. 展开更多
关键词 amino acid grape LEAF REMOVAL Sauvignon Blanc wine
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Anthocyanin profi les and color properties of red wines made from Vitis davidii and Vitis vinifera grapes 被引量:7
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作者 Yanlun Ju La Yang +5 位作者 Xiaofeng Yue Yunkui Li Rui He Shenglin Deng Xin Yang Yulin Fang 《Food Science and Human Wellness》 SCIE 2021年第3期335-344,共10页
Spine grape(Vitis davidii Foex.)is an important wild grape species native to China.Fifteen red spine grape clones and three red Vitis vinifera grape varieties were used to evaluate the differences in the anthocyanin p... Spine grape(Vitis davidii Foex.)is an important wild grape species native to China.Fifteen red spine grape clones and three red Vitis vinifera grape varieties were used to evaluate the differences in the anthocyanin profiles and color properties of wines made from V.davidii and V.vinifera grapes.Among spine wines,‘Junzi#2’wine had the highest total phenolic and total anthocyanin,‘Xiangzhenzhu’wine had the highest total flavonoids,and‘Junzi#1’wine had the highest total tannin.The anthocyanin compositions of all of the spine wines were dominated by Mv-3,5-diglucoside.The total individual anthocyanin contents in spine wines,except‘Gaoshan#5’,‘Junzi#5055’,‘Junzi#5061’,and‘Junzi#5044’,were signifi cantly higher than in V.vinifera wines.Most of the spine wines had a stronger red intensity and a brighter chroma with a bluer hue than V.vinifera wines.Correlation analysis revealed that the color properties were closely related to the anthocyanin composition.These results suggest that wines made from spine grapes may be useful for the wine industry for their color properties and high individual anthocyanin contents. 展开更多
关键词 Spine grape Vitis vinifera L. wine analysis wine color ANTHOCYANIN
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Geographic Classification of Chinese Grape Wines by Near-Infrared Reflectance Spectroscopy 被引量:1
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作者 赵芳 赵育 +1 位作者 毛文华 战吉宬 《Journal of Donghua University(English Edition)》 EI CAS 2012年第1期40-45,共6页
Near-infrared reflectance spectroscopy (NIRS) was applied to classify grape wines of different geographical origins (Changli, Huailai, and Yantai, China). Near infrared (NIR) spectra were collected in transmission mod... Near-infrared reflectance spectroscopy (NIRS) was applied to classify grape wines of different geographical origins (Changli, Huailai, and Yantai, China). Near infrared (NIR) spectra were collected in transmission mode in the wavelength range of 800-2500 nm. Wines (n=90) were randomly split into two sets, calibration set (n=54) and validation set (n=36). Discriminant analysis models were developed using BP neural network and discriminant partial least-squares discriminant analysis (PLS-DA). The prediction performance of calibration models in different wavelength range was also investigated. BP neural network models and PLS-DA models correctly classified 100% of the wines in calibration set. When used to predict wines in validation set, BP neural network models correctly classified 100%, 81.8%, and 90.9% of the wines from Changli, Huailai, and Yantai respectively, and PLS-DA models correctly classified 100% of all samples. The results demonstrated that NIRS could be used to discriminate Chinese grape wines as a rapid and reliable method. 展开更多
关键词 near-infrared reflectance spectroscopy (NIRS) Chinese grape wines discriminant analysis models BP neural network PLS-DA
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Effects of Grape Balanced Nutrition Fertilizer on Yield and Quality of Wine Grapes 被引量:1
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作者 Jianhua LUO Haisen YU +6 位作者 Lin SHI Xin KE Yu WANG Jixian DONG Zhigang GUO Donglian LI Pengzhao GAO 《Agricultural Biotechnology》 CAS 2020年第4期81-86,91,共7页
In this study,the wine grapes balanced nutrient fertilizer(BNF)was designed according to the wine grape nutritional requirements in different growth stages,and objected to reduce fertilizer application rate of wine gr... In this study,the wine grapes balanced nutrient fertilizer(BNF)was designed according to the wine grape nutritional requirements in different growth stages,and objected to reduce fertilizer application rate of wine grape cultivation,improve fertilizer utilization rate,achieve stable yield and improve the quality of wine grapes.The experiment was carried out at the Great Wall Sanggan Winery,and the Aristocratic Winery.The experiment was designed with two treatments:Namely BNF treatment,and the traditional fertilization(CK).We investigated the effects of BNF on the quality of wine grapes by examining the plant traits of wine grapes in different experimental areas during the growing season,the fruit traits at maturity,and determined the contents of VC,organic acids,amino acids,flavonoids,soluble total sugars,soluble solids,tannins,total phenols and catechins.The results show that:①The application of grape BNF greatly reduced the application amount of nitrogen,phosphorus and potassium,while the vine had no fertilizer deficiency phenomenon;②It increased the single fruit weight,the ear weight and yield to varying degrees,and the yield increase range was between 12% and 41%;③The wine grapes with BNF had the following characteristics:tight infructescence,even color,small fruit less,no fruit dropped,strong fruit colloid feeling,better aroma and taste;④It greatly improved the quality of wine grapes,and increased the content of soluble solids,soluble total sugar,organic acids,VC,total phenols,flavonoids,amino acids and tannins in fruits;⑤It greatly increased the total catechin content of"Riesling"and"Syrah",and had no significant effects on the catechin content of"Merlot"and"Cabernet Sauvignon".In summary,the application of grape BNF has certain practical significance for guiding the production of local wine grapes,ensuring the yield while improving the quality,increasing the income and reducing the environmental pollution caused by excessive fertilization. 展开更多
关键词 wine grapes Balanced nutrition fertilizer QUALITY CATECHINS Nitrogen and phosphorus reduction
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Timing of Crop Removal Has Limited Effect on Merlot Grape and Wine Composition
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作者 Petra D. King Richard E. Smart Daniel J. McClellan 《Agricultural Sciences》 2015年第4期456-465,共10页
Cluster removal during the growing season is a widely utilized vineyard management practice aiming to balance crop load to the capacity of the vine to ripen the fruit. Research was undertaken over two growing seasons ... Cluster removal during the growing season is a widely utilized vineyard management practice aiming to balance crop load to the capacity of the vine to ripen the fruit. Research was undertaken over two growing seasons (2008-2009) in Hawke’s Bay, a cool climate region of New Zealand, to establish the influence of different times of crop removal on Merlot vine growth and fruit and wine composition. The test vineyard was high-yielding, to 23 t/ha, and vigorous. A commercial standard of apical cluster thinning to remove 20 clusters per vine from vines with ca 44 clusters in 2008 and ca 47 in 2009, was carried out on uniform vigour, 7yo grafted Merlot vines at nine times between prebloom and six weeks post veraison. The 2009 season was naturally higher yielding. Timing of crop removal had no significant effect on vine vegetative growth in terms of enhanced shoot growth as measured by cane weights at pruning, or canopy leaf density. Time of thinning also had no effect on overall grape yield, cluster weight, and berry weight. There were limited effects on fruit ripeness in one season (2009) at the veraison time of thinning only, with increased Brix and lower TA levels. Berry anthocyanin concentrations were enhanced by cluster thinning in 2008, and more so when undertaken at or soon after veraison. There was however no influence of removal timing on anthocyanin levels and total phenolics in the wines. Grape ripeness, must and wine composition tended to respond more from crop removal at veraison than the other times evaluated. Data suggest that vine response was modified by excessive leafiness and shading. 展开更多
关键词 CROP Removal TIMING MERLOT grape COMPOSITION wine
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Anthocyanin Profiles in Grape Berry Skins of Different Species ofWine Grapes in Shanxi,China
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作者 Wei Tan Mingxiu Xu +5 位作者 Siqi Xie Yan Zhang Shuai Wu Qinyan Zou Qifeng Zhao Qingliang Li 《Phyton-International Journal of Experimental Botany》 SCIE 2021年第2期553-570,共18页
To understand the anthocyanin characteristics of wine grape varieties,the anthocyanin composition and content of 31 wine grape varieties were analyzed to explore the use of anthocyanins as chemical fingerprints to dis... To understand the anthocyanin characteristics of wine grape varieties,the anthocyanin composition and content of 31 wine grape varieties were analyzed to explore the use of anthocyanins as chemical fingerprints to distinguish varieties.Results showed that a total of 21 anthocyanins were detected in the skins,including cyanidin,delphinidin,petunidin,peonidin and malvidin 3-monoglucosides(or 3,5-diglucosides)along with the corresponding acetyl and p-coumaroyl derivatives.The highest and lowest total amount of anthocyanins were detected in‘Ruby Cabernet’and‘Muscat Rouge’,respectively.In the 21 Vitis vinifera grapes,there were 3~11 monoglucoside anthocyanins detected,however,there were 4 to 9 monoglucoside anthocyanins and 1~7 diglucoside anthocyanins detected in the 10 other species of grapes.Except for‘Zhesexiang’‘Seibel Noir’,‘44-6-7-1’and‘Beibinghong’,the contents of diglucoside anthocyanins in the other six varieties accounted for more than 52%of the total anthocyanins.Except for‘Zhesexiang’,‘Muscat Rouge’and‘Beibinghong’,the content of methylated anthocyanins accounted for more than 75%of total anthocyanins.There were significant differences in the anthocyanin types and contents in the skins among V.vinifera and other grapes.The results of the principal component analysis and the cluster classification of 31 grape varieties(lines)were nearly consistent,which suggested that anthocyanins can be used as chemical fingerprints to distinguish wine grape varieties. 展开更多
关键词 ANTHOCYANIN cluster analysis principal component analysis wine grape
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Terroir Suitability Zoning for the Six Prevailing Wine Grape Varieties in Ningxia Hui Autonomous Region(with a Focus on the Helan Mountain East) of China
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作者 Hongying Li Xiaoyu Zhang +1 位作者 Jing Wang Yahong Zhang 《Journal of Agricultural Science and Technology(A)》 2019年第5期278-293,共16页
Terroir plays an important role in wine grape production and good wine making.Among all the aspects of terroir in a given region,climate,soil and variety are the most important ones.The goal of this study was to evalu... Terroir plays an important role in wine grape production and good wine making.Among all the aspects of terroir in a given region,climate,soil and variety are the most important ones.The goal of this study was to evaluate these three aspects of terroir in Ningxia Hui Autonomous Region of China,with a focus on the Helan Mountain East.Based on historical weather data during the period 1981 to 2016 from 22 meteorological stations in the research area,the climatic indices were derived,with which the growable zone for wine grape cultivation in the research area was identified.By synthesizing the climatic indices and soil type data,stepwise and optimization methods were used to divide the growable zone into optimal,suitable,near-suitable and unsuitable zones for the six prevailing wine grape varieties.In the analysis,the six prevailing wine grape varieties include two white wine grape varieties of“Chardonnay”and“Riesling”and four red wine grape varieties of“Cabernet Sauvignon”,“Merlot”,“Pinot Noir”and“Grenache Noir”.With the Geographic Information System(ArcGIS)software,the geographical distributions of these terroir suitability zonings were mapped in the research area according to a small grid simulation model.The results showed that the growable zone for wine grapes was mainly focused in most of the northern and central parts of Ningxia Hui Autonomous Region.This particular zone was suitable for the typical red wine varieties like late-maturing“Grenache Noir”,medium-to-late maturing“Cabernet Sauvignon”,medium-maturing“Merlot”and early-maturing“Pinot Noir”.In particular,the optimal zone for these four red wine varieties was centered in Xixia county of Yinchuan city,Pigeon Hill of Qingtongxia city and Baima Township of Zhongning County.For the two early-to-medium maturing white wine varieties of“Riesling”and“Chardonnay”,the optimal and suitable zones were in the cool area of the central arid zone and the Qingshui River tributary. 展开更多
关键词 wine grape TERROIR Helan Mountain East China
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Evaluation of Some New Wine Grape Cultivars under S^o Francisco Valley Conditions
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作者 Patricia Coelho de Souza Leao Bruna Thais Goncalves Nunes +2 位作者 Emille Mayara Carvalho de Souza Jessica Islane de Souza Rego Jose Henrique Bemardino do Nascimento 《Journal of Agricultural Science and Technology(B)》 2017年第5期320-325,共6页
This study aimed to evaluate the performance and characteristics of the grapes as subsidies to the recommendation of new wine grape cultivars for the S^o Francisco Valley. An experiment was carried out in Petrolina, P... This study aimed to evaluate the performance and characteristics of the grapes as subsidies to the recommendation of new wine grape cultivars for the S^o Francisco Valley. An experiment was carried out in Petrolina, Pernambuco state, during four seasons in 2014 and 2015 and the treatments were represented by 10 cultivars and three breeding selections: Grenache, Tempranillo, Merlot, Cabernet Sauvignon, Malbec, Petit Verdot and Syrah for red wines and Sauvignon Blanc, Chardonnay, Chenin Blanc and breeding selections 46, 14 and 47 for white wines. The experimental design was a randomized block with four replicates and two useful plants per plot. The following variables were evaluated: sprouting, bud fertility rate, Ravaz index, production, number of clusters, weight, length and width of cluster, weight and volume of 100 berries, soluble solids and titratable acidity. A better balance between production and vigour was obtained in Grenache, Merlot and Tempranillo cultivars, presenting values of 7.56, 6.50 and 5.93, respectively, for Ravaz index. Grenache, Chenin Blanc and Tempranillo were the most productive cultivars with average yields estimated at 10.4, 8.7 and 8.4 ton/ha/season. Selection 14 had longer, wider and less compact clusters, which can reduce clusters rottenness. The percentage of sprouting ranged from 38.13% in selection 14 to 77.6% in cv. Chenin Blanc. The average index of bud fertility was 0.79 cluster/bud, with no significant differences among cultivars. Greater potential for sugar accumulation was found in the selections 14, 46 and 47, while the titratable acidity ranged from 0.63% in cv. Merlot to 0.89% in selection 47. Grenache and Tempranillo have showed potential to obtain adequate yield and balanced vines, while Sauvignon Blanc, Chardonnay, Cabemet Sauvignon, Malbec and Petit Verdot presented low yield being necessary to select clones more productive and well adapted to the tropical conditions. Further information about the quality of grapes and wines should be added to these results to allow the recommendation of new cultivars or breeding selections of wine grapes for the Sao Francisco Valley. 展开更多
关键词 Tropical viticulture wine grape yield.
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The Characters and Effect of Potassium in the Aeolian Sand Soil on Growth and Quality of Wine-Grapes in Ningxia
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作者 ZHOU Tao, LIANG Jin-xiu and SHANG Hong-ying (Institute of Soil and Fertilizer, Ningxia Academy of Agriculture-Forestry Sciences, Yinchuan 750002, P. R. China) 《Agricultural Sciences in China》 CAS CSCD 2003年第12期1345-1350,共6页
The paper reports the study on validity, absorbing and using in potassium of wine-grapes grown on aeolian sandy soil in Ningxia. The result shows that the content of slow release potassium and available potassium is l... The paper reports the study on validity, absorbing and using in potassium of wine-grapes grown on aeolian sandy soil in Ningxia. The result shows that the content of slow release potassium and available potassium is low. The amount of available potassium and fixed potassium increases with raising amount of applied potash. Slow release potassium can be quickly replenished when available potassium is depleted, but slow release potassium is exhausted too, and the buffering capacity of supplying potassium is low. The content of potassium in grains and plants increases with the increasing amount of applied K in different treatments. The sequence of potassium recovery rate in different soil is as follows: sand-loam>fine-sand>coarse-sand. The capacity of supplying potassium in high-fertility soil is better than that in low-fertility soil, and rate of potassium recovery is high. Potassium nutrient affects growth and quality of wine-grapes. 展开更多
关键词 wine-grapes Aeolian sandy soil Potassium nutrition
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On the Status quo and Development Trends of Imported Grape Wine in China
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作者 Yingchen Wang 《International English Education Research》 2014年第4期89-91,共3页
At the present stage, The imported grape wine in China have hot market, imports increased year by year, high gross mar-gins, many brands, uneven quality, low market confidence, the price value of large deviations, imp... At the present stage, The imported grape wine in China have hot market, imports increased year by year, high gross mar-gins, many brands, uneven quality, low market confidence, the price value of large deviations, imperfect legislation, regulatory confusion and so on characters. The relevant departments and units should actively take a number of measures to improve the market of imported grape wine stably and healthy development. Believe in the future, its imports would continue to grow, the gross profit margin gradually would return to rational, the cost-effective products would be prevailing, the sales channels would be diversification, the enterprises in-volved would increase, and the industry competition would become increasingly fierce. 展开更多
关键词 Imported grape wine Gross profit margin Trust degree Continues to grow
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酿酒葡萄种质资源果实品质性状的分析与评价 被引量:3
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作者 牛锐敏 许泽华 +3 位作者 沈甜 黄小晶 徐美隆 陈卫平 《中外葡萄与葡萄酒》 北大核心 2024年第1期10-19,共10页
对40份酿酒葡萄种质资源的14个果实品质性状进行统计,并应用主成分分析和聚类分析方法进行综合评价,以筛选出适栽和特色品种。结果表明,14个品质性状的变异系数在8.45%~52.73%,其中花色苷含量变异系数最大,可溶性固形物含量变异系数最小... 对40份酿酒葡萄种质资源的14个果实品质性状进行统计,并应用主成分分析和聚类分析方法进行综合评价,以筛选出适栽和特色品种。结果表明,14个品质性状的变异系数在8.45%~52.73%,其中花色苷含量变异系数最大,可溶性固形物含量变异系数最小,花色苷含量、穗质量、种子数、粒质量4个性状在不同资源之间存在较大差异;结合主要性状分布范围分析,筛选出高花色苷种质‘罗马4号’和高糖高酸高酚种质‘小芒森’。主成分分析将白色酿酒葡萄的8个主要果实性状综合为3个主成分,累计贡献率达90%以上,‘小芒森’‘菲尔斯’‘雷司令’等种质综合表现较好;红色酿酒葡萄9个品质指标综合为4个主成分,累计贡献率达87%以上,‘小味儿多’‘马瑟兰’‘赤霞珠685’‘赤霞珠’等种质综合表现较好。通过聚类分析将40份酿酒葡萄种质资源分为5类,第Ⅰ类为小粒、糖酸酚类物质含量高的种质;第Ⅲ类为大穗、糖酸酚类物质含量居中的种质;第Ⅱ类糖酸酚类物质含量处于中等,但果穗、果粒显著小于第Ⅲ类且总酚、单宁含量较高,酸含量较低;第Ⅳ类为大粒、低糖低酸低酚类种质;第Ⅴ类为中粒、低糖高酸中酚类种质。 展开更多
关键词 酿酒葡萄 种质资源 品质性状 综合评价
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马瑟兰葡萄果实生长发育和品质变化的转录组分析
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作者 付广青 刘嘉凝 +6 位作者 杨毓贤 王博 任艳华 上官凌飞 房经贵 张军翔 王西成 《南方农业学报》 CAS CSCD 北大核心 2024年第8期2310-2319,共10页
【目的】探究酿酒葡萄品种马瑟兰果实生长发育过程中的品质变化,挖掘关键的生长发育及果实品质调控基因,为马瑟兰葡萄栽培措施的精准制定和果实品质提高提供理论依据。【方法】以贺兰山东麓马瑟兰葡萄为试验材料,采集花后20 d、花后45 ... 【目的】探究酿酒葡萄品种马瑟兰果实生长发育过程中的品质变化,挖掘关键的生长发育及果实品质调控基因,为马瑟兰葡萄栽培措施的精准制定和果实品质提高提供理论依据。【方法】以贺兰山东麓马瑟兰葡萄为试验材料,采集花后20 d、花后45 d、花后75 d及花后100 d不同发育阶段的葡萄果实,测量果粒横径和纵径,测定果实可溶性固形物、总糖、可滴定酸和总酚含量,并进行转录组测序分析。【结果】随着马瑟兰葡萄果实生长发育,果实色泽加深、果粒体积增大,果实可溶性固形物和总糖含量逐渐上升,可滴定酸含量逐渐下降,总酚含量在花后20 d至花后75 d期间逐渐下降而在花后75 d至花后100 d期间略有上升。从不同发育阶段葡萄果实间共筛选到33517个DEGs,花后45 d与花后20 d比较组、花后75 d与花后45 d比较组、花后100 d与花后75 d比较组共有的DEGs为3976个。GO功能注释分析结果表明,各比较组DEGs主要注释到细胞器、细胞膜、含蛋白复合物、细胞过程和代谢过程等功能条目。KEGG信号通路富集分析结果表明,氨基酸的生物合成、糖酵解/糖异生及类黄酮生物合成等是马瑟兰葡萄果实发育过程中的重要信号通路。从富集在类黄酮生物合成信号通路的DEGs中,发现查耳酮合成酶基因(CHS)和黄烷酮-3-羟化酶基因(F3H)均在花后45 d至75 d期间上调表达,F3H在花后75 d至花后100 d期间下调表达,表明CHS与F3H基因在马瑟兰葡萄果皮着色中起重要调控作用。【结论】马瑟兰葡萄生长发育过程中伴随着果实色泽加深、果粒体积增大和生化成分的动态变化。类黄酮生物合成信号通路在葡萄转色过程中起重要作用,CHS和F3H基因影响马瑟兰葡萄果实着色及品质形成。 展开更多
关键词 马瑟兰 酿酒葡萄 果实品质 转录组测序
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酿酒葡萄新品种豫酿紫玉的选育
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作者 王鹏 贺亮亮 +5 位作者 曹阳 刘三军 杨向科 左金淼 章鹏 李永洲 《果树学报》 CAS CSCD 北大核心 2024年第5期1013-1018,共6页
豫酿紫玉葡萄是以梅鹿辄(Vitis vinifera‘Merlot’♀)和LN-33(Vitis vinifera‘LN-33’♂)为亲本经人工杂交培育而成的中早熟酿酒葡萄新品种。果穗圆锥形,偶有副穗,果粒紧凑,平均单穗质量252.70 g;果粒中等,大小均衡,平均单粒质量1.53... 豫酿紫玉葡萄是以梅鹿辄(Vitis vinifera‘Merlot’♀)和LN-33(Vitis vinifera‘LN-33’♂)为亲本经人工杂交培育而成的中早熟酿酒葡萄新品种。果穗圆锥形,偶有副穗,果粒紧凑,平均单穗质量252.70 g;果粒中等,大小均衡,平均单粒质量1.53 g;果粒近圆形,紫黑色,着色均匀,果粉中等厚,果皮中等厚,有肉囊,汁多,每果种子1~2粒;果汁无色,压榨葡萄汁呈宝石红色。成熟果可溶性固形物含量(w,后同)为21.6%,可溶性糖含量为17.46%,总酸含量为0.78%,维生素C含量为9.63 mg·100 g-1,单宁含量为4680 mg·kg-1;果实酸甜适口,果肉具有李子果香味。在郑州地区栽培,浆果于7月上旬开始着色,7月下旬始熟,8月上旬充分成熟,果实发育期为82 d;萌芽率高,生长健壮。酿酒样品酒精度、干浸出物含量、可滴定酸含量、pH值、二氧化硫残留量均在酿造干型红葡萄酒的国标范围内;酿造的干型红葡萄酒风味正,颜色佳。该品种耐寒性强,耐干旱,抗病害,丰产性好。适合在河南葡萄生态适生区和与其相似的其他葡萄生态适宜区栽培。 展开更多
关键词 酿酒葡萄 新品种 豫酿紫玉
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UV-B照射时长对酿酒葡萄果实品质及类黄酮代谢产物的影响
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作者 沈甜 牛锐敏 +2 位作者 许泽华 黄小晶 陈卫平 《食品工业科技》 CAS 北大核心 2024年第17期73-82,共10页
为探明UV-B照射时长对赤霞珠酿酒葡萄果实品质及类黄酮物质的影响,试验设立UV-B每天分别照射2、4、6、8 h共4个梯度,以不进行UV-B照射为对照(CK),对不同UV-B照射时长葡萄果实品质及类黄酮物质进行检测分析。结果表明,短时间的UV-B照射... 为探明UV-B照射时长对赤霞珠酿酒葡萄果实品质及类黄酮物质的影响,试验设立UV-B每天分别照射2、4、6、8 h共4个梯度,以不进行UV-B照射为对照(CK),对不同UV-B照射时长葡萄果实品质及类黄酮物质进行检测分析。结果表明,短时间的UV-B照射会增加果实中可溶性糖含量,UV-B不同照射时长处理的总酚、单宁、总类黄酮、黄烷-3-醇和总花色苷含量均增加;UV-B不同照射时长对酿酒葡萄果皮中的类黄酮代谢产物影响显著,筛选出差异代谢物13个,其中5种物质显著上调、8种物质显著下调;在类黄酮和次生代谢产物合成通路上分别富集了7个差异代谢物,占代谢物总数的77.78%。利用主成分对果实品质和果皮类黄酮产物测定值进行综合评价,得分从高到低依次为UV-B照射2 h>UV-B照射8 h>UV-B照射4 h>UV-B照射6 h>CK。UV-B照射酿酒葡萄赤霞珠2 h时,果实品质和类黄酮代谢产物表现最佳。 展开更多
关键词 UV-B 酿酒葡萄 果实品质 类黄酮 代谢产物
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贺兰山东麓不同酿酒葡萄品种的酚类物质及其相关性分析
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作者 马军 单守明 +1 位作者 李光宗 刘成敏 《中外葡萄与葡萄酒》 北大核心 2024年第6期40-49,共10页
以贺兰山东麓优质产区的10个白色品种和14个红色品种酿酒葡萄为研究对象,在成熟期对果实总酚和单体酚含量进行测定,并进行了相关性评价。结果表明:限于果色类型时,各类型(白品种与红品种)葡萄群体内总酚、芪类和黄烷醇分类指标变异系数... 以贺兰山东麓优质产区的10个白色品种和14个红色品种酿酒葡萄为研究对象,在成熟期对果实总酚和单体酚含量进行测定,并进行了相关性评价。结果表明:限于果色类型时,各类型(白品种与红品种)葡萄群体内总酚、芪类和黄烷醇分类指标变异系数差值<10%,各品种属性影响较小。红色品种的总酚和单体酚含量明显高于白色品种,且黄酮醇、酚酸类含量是白色品种的3.33倍和1.81倍;白色品种差异主要体现在儿茶素、儿茶素没食子酸酯、表没食子儿茶素没食子酸酯、绿原酸、咖啡酸、阿魏酸、苯甲酸、山奈酚、芦丁、柚皮苷、桑色素、槲皮素、白藜芦醇(变异系数>36%);红色品种差异主要体现在儿茶素没食子酸酯、表没食子儿茶素没食子酸酯、没食子酸、绿原酸、咖啡酸、苯甲酸、山奈酚、芦丁、柚皮苷、桑色素、槲皮素、白藜芦醇(变异系数>36%)。相关性分析表明,白色品种和红色品种葡萄单体酚类与总酚相关性最高的化合物分别为芪类和黄烷醇类。 展开更多
关键词 酿酒葡萄 成熟期 酚类物质 多样性 相关性
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桑葚葡萄复合果酒发酵工艺响应面法优化
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作者 张瑶 唐凤仙 +3 位作者 单春会 赵馨馨 赵史敏 陈新军 《中国酿造》 CAS 北大核心 2024年第9期157-163,共7页
以桑葚为主要原料,复配葡萄发酵制备桑葚葡萄复合果酒,以感官评分和酒精度为评价指标,通过单因素试验及响应面试验优化其发酵工艺,并对其发酵前后的理化指标及抗氧化活性进行测定。结果表明,与桑葚混合发酵的最适葡萄品种为玫瑰香葡萄,... 以桑葚为主要原料,复配葡萄发酵制备桑葚葡萄复合果酒,以感官评分和酒精度为评价指标,通过单因素试验及响应面试验优化其发酵工艺,并对其发酵前后的理化指标及抗氧化活性进行测定。结果表明,与桑葚混合发酵的最适葡萄品种为玫瑰香葡萄,最佳发酵工艺条件为:桑葚汁与玫瑰香葡萄质量比9:1,发酵温度20℃,初始pH为4.4,初始糖度22°Bx。在此优化条件下,桑葚葡萄复合果酒的酒精度为7.86%vol,感官评分为87.3分。发酵60 h后,总糖、pH分别从219.60 g/L、4.40下降至60.70 g/L、4.26,DPPH和羟基自由基清除率分别提升了12.32%和24.03%,总酸、总酚和总黄酮含量分别上升了1.77 g/L、159.67 mg/L和34.46 mg/L。 展开更多
关键词 桑葚 葡萄 复合果酒 响应面法 工艺优化 抗氧化活性
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橡木制品对龙眼干白葡萄酒风味物质的影响
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作者 傅晓方 陈佳威 +6 位作者 王文祺 薛楚然 赵晓宁 郜成军 凌翰 郑紫淼 张莹辉 《食品研究与开发》 CAS 2024年第13期98-105,共8页
以沙城产区龙眼葡萄为原料制备干白葡萄酒,对比研究酵母接种前添加不同类型的橡木制品对发酵龙眼干白葡萄酒的理化指标、香气成分和感官品质的影响。结果表明,对照组及不同处理组间理化指标无显著性差异;添加奶油橡木片、Pure橡木片和... 以沙城产区龙眼葡萄为原料制备干白葡萄酒,对比研究酵母接种前添加不同类型的橡木制品对发酵龙眼干白葡萄酒的理化指标、香气成分和感官品质的影响。结果表明,对照组及不同处理组间理化指标无显著性差异;添加奶油橡木片、Pure橡木片和水果橡木片的发酵龙眼干白葡萄酒分别检测出52、44、42种香气物质,气味活力值>1的物质分别有7、3、6种。添加奶油橡木片发酵龙眼干白葡萄酒的感官评分(95.7)高于添加Pure橡木片发酵龙眼干白葡萄酒的感官评分(91.5)和添加水果橡木片发酵龙眼干白葡萄酒的感官评分(92.8)。结果显示,酵母接种前添加奶油橡木片发酵龙眼干白葡萄酒的品质明显优于添加Pure橡木片和水果橡木片发酵龙眼干白葡萄酒。 展开更多
关键词 橡木制品 龙眼葡萄 干白葡萄酒 香气成分 品质分析
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地方本科高校葡萄与葡萄酒工程专业服务乡村振兴路径探析——以楚雄师范学院为例
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作者 李雅善 曹建宏 +6 位作者 崔可栩 夏婷 张磊 王贵龙 徐成东 王艳君 王家逵 《酿酒科技》 2024年第1期140-144,共5页
实施乡村振兴战略是党的十九大作出的重大决策部署,是实现中华民族伟大复兴的必由之路。地方本科高校作为重要的人才、科技高地,在服务乡村振兴战略中具有重要的作用。为了解决地方本科高校服务乡村振兴战略实施路径问题,本文在分析当... 实施乡村振兴战略是党的十九大作出的重大决策部署,是实现中华民族伟大复兴的必由之路。地方本科高校作为重要的人才、科技高地,在服务乡村振兴战略中具有重要的作用。为了解决地方本科高校服务乡村振兴战略实施路径问题,本文在分析当前地方本科高校服务乡村振兴战略中存在的问题基础上,以楚雄师范学院葡萄与葡萄酒工程专业为例,对相关实践经验和特点进行了归纳总结,以期为同类院校服务乡村振兴提供路径借鉴。 展开更多
关键词 地方本科高校 葡萄与葡萄酒工程 酿酒葡萄 乡村振兴 路径
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