Identification of hub genes associated with Helicobacter pylori infection and type 2 diabetes mellitus:A pilot bioinformatics study

BACKGROUND Helicobacter pylori(H.pylori)infection is related to various extragastric diseases including type 2 diabetes mellitus(T2DM).However,the possible mechanisms connecting H.pylori infection and T2DM remain unknown.AIM To explore potential molecular connections between H.pylori infection and T2DM.METHODS We extracted gene expression arrays from three online datasets(GSE60427,GSE27411 and GSE115601).Differentially expressed genes(DEGs)commonly present in patients with H.pylori infection and T2DM were identified.Hub genes were validated using human gastric biopsy samples.Correlations between hub genes and immune cell infiltration,miRNAs,and transcription factors(TFs)were further analyzed.RESULTS A total of 67 DEGs were commonly presented in patients with H.pylori infection and T2DM.Five significantly upregulated hub genes,including TLR4,ITGAM,C5AR1,FCER1G,and FCGR2A,were finally identified,all of which are closely related to immune cell infiltration.The gene-miRNA analysis detected 13 miRNAs with at least two gene cross-links.TF-gene interaction networks showed that TLR4 was coregulated by 26 TFs,the largest number of TFs among the 5 hub genes.CONCLUSION We identified five hub genes that may have molecular connections between H.pylori infection and T2DM.This study provides new insights into the pathogenesis of H.pylori-induced onset of T2DM.

应用Minigene剪接变异体分析技术诊断PMM2基因非经典剪接位点新变异的致病性

目的:研究Minigene剪接变异体分析技术在诊断磷酸甘露糖变位酶2(PMM2)相关先天性糖基化障碍(PMM2-CDG)中的价值,探讨磷酸甘露糖变位酶2(PMM2)基因剪接位点新变异对其转录产物的影响。方法:通过对1例PMM2-CDG患儿进行高通量测序查找可能的遗传学病因,利用Minigene剪接变异体分析技术,研究PMM2基因新剪接位点变异的致病性。根据美国医学遗传学与基因组学学会(ACMG)指南,判断新变异的致病性。结果:遗传学分析发现患儿系PMM2基因母源性c.691G>A(p.Val231Met)变异和父源性c.447+5G>A变异复合杂合子。Minigene剪接变异体分析发现:变异c.447+5G>A导致PMM2基因转录产物形成r.348_447del转录本,为致病性PMM2基因变异。患儿的临床特征为皮肤巩膜黄染,血清总胆红素、非结合胆红素和总胆汁酸明显升高,白蛋白明显降低,甲胎蛋白、铁蛋白和促甲状腺素等升高,对症支持治疗效果欠佳。结论:Minigene剪接变异体分析可为PMM2-CDG确诊和家系遗传咨询提供新的分子标记物,扩展了PMM2基因变异谱,为该病的临床诊治提供新的参考依据。

Vanillylacetone attenuates cadmium chloride-induced hippocampal damage and memory loss through upregulation of nuclear factor erythroid 2-related factor 2 gene and protein expression

Memory loss and dementia are major public health concerns with a substantial economic burden.Oxidative stress has been shown to play a crucial role in the pathophysiology of hippocampal damage-induced memory impairment.To investigate whether the antioxidant and anti-inflammatory compound vanillyla cetone(zingerone) can protect against hippocampal damage and memory loss induced by cadmium chloride(CdCl_(2)) administration in rats,we explo red the potential involvement of the nuclear factor erythroid 2-related factor 2(Nrf2) signaling pathway,which is known to modulate oxidative stress and inflammation.Sixty healt hy male Wistar rats were divided into five groups:vehicle-treated(control),vanillylacetone,CdCl_(2),vanillylacetone+ CdCl_(2),vanillylacetone+ CdCl_(2)+ brusatol(a selective pharmacological N rf2inhibitor) groups.Vanillylacetone effectively attenuated CdCl_(2)-induced damage in the dental gyrus of the hippocampus and improved the memory function assessed by the Morris Water Maze test.Additionally,vanillylacetone markedly decreased the hippocampal tissue levels of inflammatory biomarkers(interleukin-6,tumor necrosis factor-α,intracellular cell adhesive molecules) and apoptosis biomarkers(Bax and cleaved caspase-3).The control and CdCl_(2)-treated groups treated with va nillylacetone showed reduced generation of reactive oxygen species,decreased malondialdehyde levels,and increased superoxide dismutase and glutathione activities,along with significant elevation of nuclear Nrf2 mRNA and protein expression in hippocampal tissue.All the protective effects of vanillylacetone we re substantially blocked by the co-administration of brusatol(a selective N rf2 inhibitor).Va nillylacetone mitigated hippocampal damage and memory loss induced by CdCl_(2),at least in part, by activating the nuclear transcription factor Nrf2.Additionally,vanillylacetone exerted its potent antioxidant and antiinflammatory actions.

To Analyze the Sensitivity of RT-PCR Assays Employing S Gene Target Failure with Whole Genome Sequencing Data during Third Wave by SARS-CoV-2 Omicron Variant

Introduction: Omicron is a highly divergent variant of concern (VOCs) of a severe acute respiratory syndrome SARS-CoV-2. It carries a high number of mutations in its spike protein hence;it is more transmissible in the community by immune evasion mechanisms. Due to mutation within S gene, most Omicron variants have reported S gene target failure (SGTF) with some commercially available PCR kits. Such diagnostic features can be used as markers to screen Omicron. However, Whole Genome Sequencing (WGS) is the only gold standard approach to confirm novel microorganisms at genetically level as similar mutations can also be found in other variants that are circulating at low frequencies worldwide. This Retrospective study is aimed to assess RT-PCR sensitivity in the detection of S gene target failure in comparison with whole genome sequencing to detect variants of Omicron. Methods: We have analysed retrospective data of SARS-CoV-2 positive RT-PCR samples for S gene target failure (SGTF) with TaqPath COVID-19 RT-PCR Combo Kit (ThermoFisher) and combined with sequencing technologies to study the emerged pattern of SARS-CoV-2 variants during third wave at the tertiary care centre, Surat. Results: From the first day of December 2021 till the end of February 2022, a total of 321,803 diagnostic RT-PCR tests for SARS-CoV-2 were performed, of which 20,566 positive cases were reported at our tertiary care centre with an average cumulative positivity of 6.39% over a period of three months. In the month of December 21 samples characterized by the SGTF (70/129) were suggestive of being infected by the Omicron variant and identified as Omicron (B.1.1.529 lineage) when sequence. In the month of January, we analysed a subset of samples (n = 618) with SGTF (24%) and without SGTF (76%) with Ct values Conclusions: During the COVID-19 pandemic, it took almost more than 15 days to diagnose infection and identify pathogen by sequencing technology. In contrast to that molecular assay provided quick identification with the help of SGTF phenomenon within 5 hours of duration. This strategy helps scientists and health policymakers for the quick isolation and identification of clusters. That ultimately results in a decreased transmission of pathogen among the community.

Analysis of SMOC2 gene variants in familial and nonfamilial primary open angle glaucoma Pakistani patients

AIM:To find out the association of secreted protein acidic and rich in cysteine(SPARC)-related modular calcium binding 2(SMOC2)gene variants rs2255680 and rs13208776 with genotypic and phenotypic characteristics in both familial and non-familial primary open angle glaucoma(POAG)patients.METHODS:A total of 212 POAG patients,comprising 124 familial and 88 non-familial,were enrolled.For genotyping the SMOC2 variant rs2255680,amplification refractory mutation system(ARMS)-polymerase chain reaction(PCR)method and PCR-restriction fragment length polymorphism(PCR-RFLP)were utilized for analyzing rs13208776 variant.RESULTS:The mean age of familial POAG patients was 50.92±9.12y,with 78 males and 46 females.The mean age of non-familial POAG patients was 53.14±13.44y,with 52 males and 36 females.The SMOC2 gene variant rs13208776 showed the significant association with POAG between familial and non-familial groups.The homozygous G/G variant was frequent among non-familial(60.2%)whereas the heterozygous G/A variant was more frequent in familial POAG patients(46%).There were significant differences in G/A variant between familial and non-familial glaucoma patients,and the risk was decreased to 0.53-fold in non-familial glaucoma patients[odds ratio(OR):0.53;95%confidence interval(CI):0.29-0.94;P=0.033]in codominant model.The risk was further reduced to 0.49-fold(95%CI:0.28-0.86;P=0.012)in dominant model for non-familial patients.No significant association of SMOC2 gene variant rs2255680 between familial and non-familial glaucoma patients was found in our population.The haplotype analysis showed the decreased risk for TA[OR:0.48(95%CI:0.29-0.79);P=0.004]and an increased risk for TG[OR=2.28(95%CI:1.22-4.25);P=0.01]haplotypes.CONCLUSION:Current findings show significant association of SMOC2 gene variant rs13208776 with POAG between familial and non-familial Pakistani patients.

AAV2-PDE6B restores retinal structure and function in the retinal degeneration 10 mouse model of retinitis pigmentosa by promoting phototransduction and inhibiting apoptosis

Retinitis pigmentosa is a group of inherited diseases that lead to retinal degeneration and photoreceptor cell death.However,there is no effective treatment for retinitis pigmentosa caused by PDE6B mutation.Adeno-associated virus(AAV)-mediated gene therapy is a promising strategy for treating retinitis pigmentosa.The aim of this study was to explore the molecular mechanisms by which AAV2-PDE6B rescues retinal function.To do this,we injected retinal degeneration 10(rd10)mice subretinally with AAV2-PDE6B and assessed the therapeutic effects on retinal function and structure using dark-and light-adapted electroretinogram,optical coherence tomography,and immunofluorescence.Data-independent acquisition-mass spectrometry-based proteomic analysis was conducted to investigate protein expression levels and pathway enrichment,and the results from this analysis were verified by real-time polymerase chain reaction and western blotting.AAV2-PDE6B injection significantly upregulated PDE6βexpression,preserved electroretinogram responses,and preserved outer nuclear layer thickness in rd10 mice.Differentially expressed proteins between wild-type and rd10 mice were closely related to visual perception,and treating rd10 mice with AAV2-PDE6B restored differentially expressed protein expression to levels similar to those seen in wild-type mice.Kyoto Encyclopedia of Genes and Genome analysis showed that the differentially expressed proteins whose expression was most significantly altered by AAV2-PDE6B injection were enriched in phototransduction pathways.Furthermore,the phototransductionrelated proteins Pde6α,Rom1,Rho,Aldh1a1,and Rbp1 exhibited opposite expression patterns in rd10 mice with or without AAV2-PDE6B treatment.Finally,Bax/Bcl-2,p-ERK/ERK,and p-c-Fos/c-Fos expression levels decreased in rd10 mice following AAV2-PDE6B treatment.Our data suggest that AAV2-PDE6B-mediated gene therapy promotes phototransduction and inhibits apoptosis by inhibiting the ERK signaling pathway and upregulating Bcl-2/Bax expression in retinitis pigmentosa.

Regulatory potential of soil available carbon,nitrogen,and functional genes on N_(2)O emissions in two upland plantation systems

Dynamic nitrification and denitrification processes are affected by changes in soil redox conditions,and they play a vital role in regulating soil N_(2)O emissions in rice-based cultivation.It is imperative to understand the influences of different upland crop planting systems on soil N_(2)O emissions.In this study,we focused on two representative rotation systems in Central China:rapeseed–rice(RR)and wheat–rice(WR).We examined the biotic and abiotic processes underlying the impacts of these upland plantings on soil N_(2)O emissions.The results revealed that during the rapeseed-cultivated seasons in the RR rotation system,the average N_(2)O emissions were 1.24±0.20 and 0.81±0.11 kg N ha^(–1)for the first and second seasons,respectively.These values were comparable to the N_(2)O emissions observed during the first and second wheat-cultivated seasons in the WR rotation system(0.98±0.25 and 0.70±0.04 kg N ha^(–1),respectively).This suggests that upland cultivation has minimal impacts on soil N_(2)O emissions in the two rotation systems.Strong positive correlations were found between N_(2)O fluxes and soil ammonium(NH_(4)^(+)),nitrate(NO_(3)^(–)),microbial biomass nitrogen(MBN),and the ratio of soil dissolved organic carbon(DOC)to NO_(3)^(–)in both RR and WR rotation systems.Moreover,the presence of the AOA-amoA and nirK genes were positively associated with soil N_(2)O fluxes in the RR and WR systems,respectively.This implies that these genes may have different potential roles in facilitating microbial N_(2)O production in various upland plantation models.By using a structural equation model,we found that soil moisture,mineral N,MBN,and the AOA-amoA gene accounted for over 50%of the effects on N_(2)O emissions in the RR rotation system.In the WR rotation system,soil moisture,mineral N,MBN,and the AOA-amoA and nirK genes had a combined impact of over 70%on N_(2)O emissions.These findings demonstrate the interactive effects of functional genes and soil factors,including soil physical characteristics,available carbon and nitrogen,and their ratio,on soil N_(2)O emissions during upland cultivation seasons under rice-upland rotations.

Pathogenesis of chronic enteropathy associated with the SLCO2A1 gene:Hypotheses and conundrums

Chronic enteropathy associated with the SLCO2A1 gene(CEAS)is a complex gastroenterological condition characterized by multiple ulcers in the small intestine with chronic bleeding and protein loss.This review explores the potential mechanisms underlying the pathogenesis of CEAS,focusing on the role of SLCO2A1-encoded prostaglandin transporter OATP2A1 and its impact on prostaglandin E2(PGE2)levels.Studies have suggested that elevated PGE2 levels contribute to mucosal damage,inflammation,and disruption of the intestinal barrier.The effects of PGE2 on macrophage activation and Maxi-Cl channel functionality,as well as its interaction with nonsteroidal anti-inflammatory drugs play crucial roles in the progression of CEAS.Understanding the balance between its protective and pro-inflammatory effects and the complex interactions within the gastrointestinal tract can shed light on potential therapeutic targets for CEAS and guide the development of novel,targeted therapies.

Transglutaminase 2 serves as a pathogenic hub gene of KRAS mutant colon cancer based on integrated analysis

BACKGROUND Colon cancer is acknowledged as one of the most common malignancies worldwide,ranking third in United States regarding incidence and mortality.Notably,approximately 40%of colon cancer cases harbor oncogenic KRAS mutations,resulting in the continuous activation of epidermal growth factor receptor signaling.AIM To investigate the key pathogenic genes in KRAS mutant colon cancer holds considerable importance.METHODS Weighted gene co-expression network analysis,in combination with additional bioinformatics analysis,were conducted to screen the key factors driving the progression of KRAS mutant colon cancer.Meanwhile,various in vitro experiments were also conducted to explore the biological function of transglutaminase 2(TGM2).RESULTS Integrated analysis demonstrated that TGM2 acted as an independent prognostic factor for progression-free survival.Immunohistochemical analysis on tissue microarrays revealed that TGM2 was associated with an elevated probability of perineural invasion in patients with KRAS mutant colon cancer.Additionally,biological roles of the key gene TGM2 was also assessed,suggesting that the downregulation of TGM2 attenuated the proliferation,invasion,and migration of the KRAS mutant colon cancer cell line.CONCLUSION This study underscores the potential significance of TGM2 in the progression of KRAS mutant colon cancer.This insight not only offers a theoretical foundation for therapeutic approaches but also highlights the need for additional clinical trials and fundamental research to support our preliminary findings.

Gene expression analysis of cytokines and MMPs in melatonin and rhBMP-2 enhanced bone remodeling

BACKGROUND In the medical and dental fields,there is a need for studies of new therapeutic approaches for the treatment of bone defects that cause extensive bone loss.Melatonin may be an important endogenous biological factor for bone remodeling,and growth factors may enhance the repair process.AIM To evaluate the gene expression of cytokines(IL-1β,IL-6,IL-10 and TNF-α),markers of osteoclastogenesis(RANK,RANKL and OPG)and MMPs(MMP-1,MMP-2,MMP-8 and MMP-13)from the treatment of melatonin associated with an osteogenic membrane and rhBMP-2 on the recovery of a bone injury.METHODS Sixty-four rats were used and divided into 9 experimental groups and were formed according to the treatment carried out in the region of the bone lesion,which varied between the combination of 1,10 and 100μmol/L of melatonin.Gene Expression analysis was performed using real time-PCR by reading the concentration of total RNA and reverse transcription.RESULTS There were differences between groups when compared with clot or scaffold control,and improvement with a higher concentration of melatonin or rhBMP-2.The combination melatonin(1μg)with 5μg of rhBMP-2,using the guided bone regeneration technique,demonstrated some effects,albeit mild,on bone repair of critical bone defects.CONCLUSION This indicates that the approach for administering these substances needs to be reassessed,with the goal of ensuring their direct application to the affected area.Therefore,future research must be carried out,seeking to produce materials with these ideal characteristics.

Identification of M2 macrophage-related genes for establishing a prognostic model in pancreatic cancer: FCGR3A as key gene

Background:Pancreatic ductal adenocarcinoma(PDAC)has a rich and complex tumor immune microenvironment(TIME).M2 macrophages are among the most extensively infiltrated immune cells in the TIME and are necessary for the growth and migration of cancers.However,the mechanisms and targets mediating M2 macrophage infiltration in pancreatic cancer remain elusive.Methods:The M2 macrophage infiltration score of patients was assessed using the xCell algorithm.Using weighted gene co-expression network analysis(WGCNA),module genes associated with M2 macrophages were identified,and a predictive model was designed.The variations in immunological cell patterns,cancer mutations,and enrichment pathways between the cohorts with the high-and low-risk were examined.Additionally,the expression of FCGR3A and RNASE2,as well as their association with M2 macrophages were evaluated using the HPA,TNMplot,and GEPIA2 databases and verified by tissue immunofluorescence staining.Moreover,in vitro cell experiments were conducted,where FCGR3A was knocked down in pancreatic cancer cells using siRNA to analyze its effects on M2 macrophage infiltration,tumor proliferation,and metastasis.Results:The prognosis of patients in high-risk and low-risk groups was successfully distinguished using a prognostic risk score model of M2 macrophage-related genes(p=0.024).Between the high-and low-risk cohorts,there have been notable variations in immune cell infiltration patterns,tumor mutations,and biological functions.The risk score was linked to the manifestation of prevalent immunological checkpoints,immunological scores,and stroma values(all p<0.05).In vitro experiments and tissue immunofluorescence staining revealed that FCGR3A can promote the infiltration or polarization of M2 macrophages and enhance tumor proliferation and migration.Conclusions:In this study,an M2 macrophage-related pancreatic cancer risk score model was established,and found that FCGR3A was correlated with tumor formation,metastasis,and M2 macrophage infiltration.

丛枝菌根真菌对褐土玉米氮素吸收和土壤N_(2)O排放的影响

探究不同氮肥水平下丛枝菌根(AM)真菌对褐土玉米土壤N_(2)O排放和氮转化功能基因的影响,为阐明AM真菌在褐土N_(2)O排放中的作用和效应提供理论依据。设置氮肥用量(NⅠ:105 mg/kg;NⅡ:210 mg/kg)、AM真菌(M0:不接种AM真菌;M1:接种根内根孢囊霉(Rhizophagus intraradices);M2:接种摩西斗管囊霉(Funneliformis mosseae);M3:接种Rhizophagus intraradices+Funneliformis mosseae等比例混合)双因素盆栽试验。测定植株地上部全氮含量、土壤铵态氮、硝态氮含量和N_(2)O排放量,采用实时荧光定量聚合酶链式反应(PCR)法分析土壤硝化功能基因(amoA-AOA和amoA-AOB)和反硝化功能基因(nirS、nirK和nosZ)的丰度。结果表明,两种施氮水平下,接种AM真菌均可显著降低土壤N_(2)O排放通量和累积排放量,不同AM真菌处理下N_(2)O累积排放量表现为:M0>M2>M1>M3。相同AM真菌处理的土壤N_(2)O排放通量和累积排放量在NⅡ施氮水平高于NⅠ施氮水平;相同AM真菌处理的玉米菌根侵染率在NⅡ施氮水平低于NⅠ施氮水平。与M0相比,NⅠ条件下M1、M2和M3处理土壤铵态氮含量分别降低24.5%、20.8%和45.3%,硝态氮含量分别降低19.7%、14.9%和30.2%,植株地上部全氮含量分别增加16.3%、35.2%和59.6%;与M0相比,NⅡ条件下M1、M2和M3处理土壤铵态氮含量分别降低20.9%、24.8%和40.0%,硝态氮含量分别降低36.3%、25.6%和45.2%,植株地上部全氮含量分别增加33.2%、43.9%和95.4%。两种施氮水平下,AM真菌可显著降低土壤硝化功能基因(amoA-AOA和amoA-AOB)丰度,增加反硝化功能基因(nirS、nirK和nosZ)丰度。AM真菌与N_(2)O排放通量呈极显著负相关。本盆栽试验条件下,接种AM真菌均可增强两种氮肥用量玉米植株氮素吸收能力,调节硝化、反硝化相关功能基因的丰度,减少土壤N_(2)O气体的排放,且两种AM真菌混合处理的N_(2)O减排效应强于单一AM真菌接种。

腺苷脱氨酶2缺乏症临床特征与基因型分析

目的总结3例腺苷脱氨酶2(ADA 2)缺乏症患儿的临床特征及基因型特点,提高对该病的认识。方法回顾性分析3例ADA2缺乏症患儿的临床特点并利用全外显子测序进行遗传学分析。利用试剂盒测定患儿血浆中ADA2酶的活性。总结该病的临床及基因型特征。结果本组3例患儿均存在ADA2基因变异,例1以反复发热、皮疹、惊厥为主要临床表现,合并脑卒中,伴炎症指标明显升高,ADA2基因存在复合杂合变异:c.139G>T和c.484T>C突变。例2以反复发热、皮疹为主要临床表现,病程中合并消化道穿孔、脑卒中,炎症指标明显升高。WES检测发现ADA2基因存在c.916C>T及c.1069G>A复合杂合突变。例3以反复发热、咳嗽为主要临床表现,合并心肌炎,伴免疫功能明显下降;WES检测发现患者ADA2基因存在c.849T>G纯合突变。血浆ADA2酶活性测定发现例1和2酶活性显著降低。结论ADA2缺乏症国内罕见,临床特征多变,掌握其临床特征及基因特点,有助于提高诊断水平。

鉴别猪圆环病毒2型和3型双重TaqMan MGB探针FQ-PCR检测方法研究

建立一种快速、特异鉴别检测猪圆环病毒2型(PCV2)和猪圆环病毒3型(PCV3)的双重TaqMan MGB探针FQ-PCR方法,本研究以PCV2的Rep蛋白和PCV3的Cap蛋白基因作为靶基因,各设计1对特异性引物和1条TaqMan MGB探针,经优化各反应条件和进行敏感性、特异性、重复性和干扰性试验,建立鉴别检测PCV2/PCV3的双重FQ-PCR方法。结果显示:该方法可特异性扩增PCV2、PCV3核酸,与猪伪狂犬病病毒(PRV)等8种病原及阴性对照无交叉反应,特异性较强;对PCV2和PCV3阳性质粒标准品的最低检出限均可达10 copies/μL,敏感性较高;PCV2/PCV3批内/批间重复试验变异系数(CV)值均在3%以下,表明方法稳定性、重复性较好;干扰性试验表明在两种病毒阳性质粒起始模板相差较大时该方法不会影响对其中任一病毒核酸的检出和准确定量。对42份临床疑似PCV感染样品检测结果与PCV2、PCV3基因测序结果符合率100%。本研究建立的双重FQ-PCR方法具有敏感性高达10 copies/μL、特异性强、在同一反应体系中能同时快速鉴别检测PCV2、PCV3等优点,可用于临床PCV2/PCV3感染的快速鉴别检测。

基于心功能及IGFBP7、sST2、CGRP、ET分析沙库巴曲缬沙坦在治疗冠心病合并慢性心力衰竭中的应用效果

目的 分析冠心病(CHD)合并慢性心力衰竭(CHF)患者应用沙库巴曲缬沙坦治疗的效果。方法 选择2020年1月至2023年1月邯郸市第四医院收治的86例CHD合并CHF患者,以随机数字表法将其分为对照组和试验组各43例。两组CHD治疗均应用硝酸酯类、他汀类及抗血小板药物,对照组CHF治疗应用坎地沙坦酯片、醛固酮受体拮抗剂及β受体阻滞剂,试验组治疗则将对照组中的坎地沙坦酯片替换为沙库巴曲缬沙坦钠片。比较两组疗效、不良反应、心功能指标[左室短轴缩短率(LVFS)、左室射血分数(LVEF)、6min步行距离(6 MWD)]、心室重构指标[Ⅲ型胶原前肽(PⅢP)、层粘蛋白(LN)、基质金属蛋白酶-9(MMP-9)]、心肌损伤和血管内皮功能相关指标[胰岛素样生长因子结合蛋白7(IGFBP7)、可溶性生长刺激表达基因2(sST2)、降钙素基因相关肽(CGRP)、内皮素(ET)]。结果与对照组比,试验组治疗3个月后的总有效率更高,差异有统计学意义(P<0.05)。两组治疗3个月后的LVFS、LVEF、6 MWD、IGFBP7、CGRP与治疗前比升高,且试验组与对照组比更高,差异有统计学意义(P<0.05);PⅢP、LN、MMP-9、sST2、ET降低,试验组与对照组比更低,差异有统计学意义(P<0.05)。两组不良反应总发生率对比差异无统计学意义(P>0.05)。结论 沙库巴曲缬沙坦可有效调节CHD合并CHF患者IGFBP7、sST2、CGRP、ET,改善血管内皮功能、心肌损伤、心室重构及心功能,进而可提高疗效,且具有良好的安全性。

2型糖尿病合并骨质疏松患者PTH及ER基因多态性分析

目的探讨2型糖尿病(type 2 diabetes mellitus,T2DM)合并骨质疏松(osteoporosis,OP)患者甲状旁腺素(parathyroid hormone,PTH)及雌激素受体(estrogen receptor,ER)基因多态性特点。方法选取2022年11月至2023年10月在昆明市第一人民医院内分泌科住院的来自昆明地区T2DM患者110例,根据骨密度结果将其分组为T2DM无OP组(n=68,T≥-1.0)、T2DM伴OP组(n=42,T≤-2.5),检测其PTH及ER基因型及等位基因频率,比较其与性别、身高、体重等临床指标间的差异。结果回归分析显示,T2DM合并OP与患者性别、体重相关(P<0.05),而PTH基因、ER基因多态性则无相关性(P>0.05)。结论性别和体重是T2DM患者骨质疏松发生的独立危险因素;PTH基因、ER基因多态性与昆明地区T2DM伴OP的遗传易感性无关。

PRRT2基因突变相关癫痫患儿临床及基因突变特点分析

目的分析富脯氨酸跨膜蛋白2(PRRT2)基因突变相关癫痫患儿临床及基因突变特点。方法回顾性分析2015年1月至2020年8月广西壮族自治区妇幼保健院收治的17例PRRT2基因突变相关癫痫患儿的临床资料,对其临床及基因突变特点进行总结分析。结果17例患儿中男9例,女8例,中位起病龄为5月龄。癫痫发作类型多样,10例起病初期有丛集性发作。诊断为良性婴儿癫痫(BIE)8例,良性家族性婴儿癫痫(BFIE)4例,婴儿痉挛症(IS)1例,BFIE+发作性运动诱发性运动障碍(PKD)1例。应用抗癫痫发作药物单药治疗后大部分患儿病情控制良好。截至末次随访,16例癫痫发作已缓解,1例仍有发作。4例出现认知发育落后。基因检测结果显示17例患儿中有8例为PRRT2基因整体缺失,9例为PRRT2基因点突变,均为移码突变,其中8例突变位点为c.640_641insC。7例为家族遗传性突变,2例为新生突变。结论PRRT2基因突变相关癫痫绝大多数在生后6个月内起病,病初以丛集性发作为特点。癫痫发作类型多样,表现轻重不一。抗癫痫发作药物单药治疗多能有效控制癫痫发作,少部分患儿有不同程度的认知发育落后。c.640_641insC可能是PRRT2基因的热点突变位点。

乳腺癌患者病理特征与Bcl-2、CXCL13、PAX8表达情况的关系分析

目的分析乳腺癌患者病理特征与B细胞淋巴瘤/白血病-2基因(Bcl-2)、趋化因子配体13(CXCL13)、配对盒基因8抗体(PAX8)表达情况的关系。方法收集2021年1月至2023年1月该院收治的160例乳腺癌患者临床资料。采用免疫组化法对其癌组织与癌旁组织Bcl-2、CXCL13、PAX8表达情况进行检测,并分析3项指标与患者病理特征的关系。结果与癌旁组织比较,癌组织Bcl-2、CXCL13、PAX8阳性率更高,差异有统计学意义(P<0.05)。与雌激素受体(ER)阴性、肿瘤最大径≥3 cm、孕激素受体(PR)阴性患者比较,ER阳性、肿瘤最大径<3 cm、PR阳性患者中Bcl-2高表达占比更高,差异有统计学意义(P<0.05);与无淋巴结转移、Ⅰ~Ⅱ期患者比较,淋巴结转移、Ⅲ~Ⅳ期患者中CXCL13高表达占比更高,差异有统计学意义(P<0.05);与Ⅰ~Ⅱ期、高/中分化、无淋巴结转移患者比较,Ⅲ~Ⅳ期、低分化、有淋巴结转移患者中PAX8高表达占比更高,差异有统计学意义(P<0.05)。ER、PR表达情况与Bcl-2表达情况呈正相关(P<0.05),肿瘤最大径与Bcl-2表达情况呈负相关(P<0.05);临床分期、淋巴结转移情况与CXCL13、PAX8表达情况呈正相关(P<0.05);分化程度与PAX8表达情况呈负相关(P<0.05)。结论乳腺癌患者Bcl-2、CXCL13、PAX8表达情况对疾病的发生和发展具有明显影响,有望成为评估乳腺癌患者病情严重程度的标志物。

LncRNA CASC2靶向调控miR-155-5p及其对HCC细胞恶性表型的影响

目的:长链非编码RNA(long non-coding RNA,lncRNA)癌易感性候选基因2(cancer susceptibility candidate gene 2,CASC2)在卵巢癌、肝细胞癌(hepatocellular carcinoma,HCC)等多种恶性肿瘤中发挥抑癌基因的作用,但其在HCC中的作用机制尚未完全清楚。本研究旨在分析CASC2在HCC组织中的表达及临床意义,并探讨CASC2对人肝癌细胞系Huh-7细胞恶性表型的影响及其可能的作用机制。方法:采用real-time RT-PCR检测HCC组织和HCC细胞系中CASC2和miR-155-5p的表达水平,分析CASC2的表达水平与HCC患者临床病理特征及预后的关系;采用双荧光素酶报告基因实验检测CASC2和miR-155-5p的靶向关系;将pcDNA3.1-CASC2重组质粒、pcDNA3.1空质粒、miR-155-5p模拟物(mimic)及miR-155-5p模拟物的阴性对照(miR-NC)分别或共同转染至Huh-7细胞中,并根据感染物的不同将细胞分为空白对照组、pcDNA3.1组、pcDNA3.1-CASC2组、pcDNA3.1-CASC2+miR-155-5p组、pcDNA3.1-CASC2+miR-NC组,再分别采用四甲基噻唑蓝(methylthiazolyl tetrazolium,MTT)法、Annexin V/碘化丙啶(propidium iodide,PI)双染色法、Transwell实验检测CASC2靶向调控miR-155-5p对Huh-7细胞增殖、凋亡、迁移及侵袭的影响。结果:CASC2在HCC组织和细胞系中均表达下调,miR-155-5p在HCC组织和细胞系中均表达水平升高,两者呈显著负相关(r=−0.388,P<0.05)。HCC患者中CASC2的表达与甲胎蛋白水平、肿瘤大小、TNM分期、分化程度、肝内转移及不良预后均密切相关(均P<0.05)。CSAC2靶向负调控miR-155-5p的表达,pcDNA3.1-CASC2组细胞增殖活力显著低于空白对照组和pcDNA3.1组(均P<0.05);与pcDNA3.1-CASC2组相比,pcDNA3.1-CASC2+miR-155-5p组细胞的增殖活力增强,差异具有统计学意义(P<0.05)。pcDNA3.1-CASC2组细胞凋亡率明显高于空白对照组和pcDNA3.1组(均P<0.05);与pcDNA3.1-CASC2组相比,pcDNA3.1-CASC2+miR-155-5p组细胞的凋亡率显著降低(P<0.05)。与空白对照组和pcDNA3.1组相比,pcDNA3.1-CASC2组迁移和侵袭细胞数量均显著降低(均P<0.05);与pcDNA3.1-CASC2组相比,pcDNA3.1-CASC2+miR-155-5p组迁移和侵袭的细胞数量均显著升高(均P<0.05)。结论:CASC2在HCC组织和细胞中呈低表达,与HCC预后密切相关,过表达CASC2可通过靶向调控miR-155-5p抑制肝癌细胞的增殖、迁移和侵袭,促进细胞凋亡。

密码子优化和信号肽对猪圆环病毒ORF2基因在毕赤酵母表达中的影响

为了在毕赤酵母中高效表达PCV2ORF2基因,对PCV2ORF2基因编码的187个氨基酸密码子进行毕赤酵母偏爱性优化,利用两步PCR全化学合成优化后的ORF2基因。将优化前后的PCV2ORF2基因片段插入毕赤酵母表达载体pPICZα-C,构建重组质粒pPICZα-C-ORF2和p PICZα-C-ORF2op。将线性化的重组质粒电转化毕赤酵母GS115,并筛选Zeocin抗性重组子。同时,也研究了信号肽对PCV2ORF2表达效率的影响。SDS-PAGE和Western-blot分析结果表明,只有经密码子优化且去除ORF2基因自身信号肽的重组子能够分泌表达PCV2 ORF2蛋白,目的蛋白分子质量约为30 ku,表达的重组ORF2蛋白可被PCV2阳性血清识别,具有生物学活性。