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New Progress regarding the Use of Lactic Acid Bacteria as Live Delivery Vectors, Treatment of Diseases and Induction of Immune Responses in Different Host Species Focusing on <i>Lactobacillus</i>Species 被引量:5
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作者 Seria Masole Shonyela Guan Wang +2 位作者 Wentao Yang Guilian Yang Chunfeng Wang 《World Journal of Vaccines》 2017年第4期43-75,共33页
Lactobacillus species are non-spore-forming, gram-positive bacteria and lactic acid producing bacteria (LAPB) that naturally inhabit the human and animal gastrointestinal and mouth organs. The aim of this review was t... Lactobacillus species are non-spore-forming, gram-positive bacteria and lactic acid producing bacteria (LAPB) that naturally inhabit the human and animal gastrointestinal and mouth organs. The aim of this review was to evaluate the new progress regarding the use of Lactobacillus species as live delivery vectors, prevention, and treatment of pathogenic and metabolic diseases. Lactobacillus strains of probiotics have been extensively studied and have confirmed that they can absolutely improve performance as live delivery vectors, a treatment option of various diseases such as: Hemorrhagic cecal coccidiosis in young poultry, hypertension, avian flu, obesity, diabetes, Derzsy’s disease or parvovirus infection, human immunodeficiency virus infections, irritable bowel syndrome, gastrointestinal disorders, Fungal infections, vaginal eubiosis, fish and shellfish species diseases. We give you an idea about that Lactobacillus species have been proficient in preventing and treating both disorders in animal models and some are used for clinical trials. We present the most current studies on the use of Lactobacillus strains that had an impact on an effective immune response to a specific antigen because a variety of antigens have been expressed. Therefore Lactobacillus strains can be considered as good candidates because of its potential for diseases treatment and vaccine development as heterologous protein secretion to date. 展开更多
关键词 LACTOBACILLI Probiotics Lactic Acid LIVE delivery vectorS TREATMENT Immune Responses
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Insights on drug and gene delivery systems in liver fibrosis
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作者 Kunj Vyas Mayur M Patel 《Asian Journal of Pharmaceutical Sciences》 SCIE CAS 2023年第2期4-27,共24页
Complications of the liver are amongst the world’s worst diseases.Liver fibrosis is the first stage of liver problems,while cirrhosis is the last stage,which can lead to death.The creation of effective anti-fibrotic ... Complications of the liver are amongst the world’s worst diseases.Liver fibrosis is the first stage of liver problems,while cirrhosis is the last stage,which can lead to death.The creation of effective anti-fibrotic drug delivery methods appears critical due to the liver’s metabolic capacity for drugs and the presence of insurmountable physiological impediments in the way of targeting.Recent breakthroughs in anti-fibrotic agents have substantially assisted in fibrosis;nevertheless,the working mechanism of anti-fibrotic medications is not fully understood,and there is a need to design delivery systems that are well-understood and can aid in cirrhosis.Nanotechnology-based delivery systems are regarded to be effective but they have not been adequately researched for liver delivery.As a result,the capability of nanoparticles in hepatic delivery was explored.Another approach is targeted drug delivery,which can considerably improve efficacy if delivery systems are designed to target hepatic stellate cells(HSCs).We have addressed numerous delivery strategies that target HSCs,which can eventually aid in fibrosis.Recently genetics have proved to be useful,and methods for delivering genetic material to the target place have also been investigated where different techniques are depicted.To summarize,this review paper sheds light on themost recent breakthroughs in drug and gene-based nano and targeted delivery systems that have lately shown useful for the treatment of liver fibrosis and cirrhosis. 展开更多
关键词 Liver fibrosis Gene delivery system Nano drug delivery system CIRRHOSIS Targeted delivery system vector
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CRISPR/Cas9 systems:Delivery technologies and biomedical applications
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作者 Yimin Du Yanfei Liu +2 位作者 Jiaxin Hu Xingxing Peng Zhenbao Liu 《Asian Journal of Pharmaceutical Sciences》 SCIE CAS 2023年第6期1-31,共31页
The emergence of the clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated protein 9(Cas9)genome-editing system has brought about a significant revolution in the realm of managing human d... The emergence of the clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated protein 9(Cas9)genome-editing system has brought about a significant revolution in the realm of managing human diseases,establishing animal models,and so on.To fully harness the potential of this potent gene-editing tool,ensuring efficient and secure delivery to the target site is paramount.Consequently,developing effective delivery methods for the CRISPR/Cas9 system has become a critical area of research.In this review,we present a comprehensive outline of delivery strategies and discuss their biomedical applications in the CRISPR/Cas9 system.We also provide an indepth analysis of physical,viral vector,and non-viral vector delivery strategies,including plasmid-,mRNA-and protein-based approach.In addition,we illustrate the biomedical applications of the CRISPR/Cas9 system.This review highlights the key factors affecting the delivery process and the current challenges facing the CRISPR/Cas9 system,while also delineating future directions and prospects that could inspire innovative delivery strategies.This review aims to provide new insights and ideas for advancing CRISPR/Cas9-based delivery strategies and to facilitate breakthroughs in biomedical research and therapeutic applications. 展开更多
关键词 CRISPR/Cas9 Physical delivery Viral vector Non-viral vector Biomedical applications
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Synthesis and Properties of Chitosan-PEI Graft Copolymers as Vectors for Nucleic Acid Delivery
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作者 Wing-Fu Lai Marie Chia-Mi Lin 《材料科学与工程(中英文版)》 2010年第12期34-40,共7页
关键词 DNA载体 接枝共聚物 壳聚糖 核酸 体外细胞毒性 传递 性能 合成
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基因治疗递送系统的研究新进展:遗传性视网膜疾病治疗的曙光
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作者 翟杨 苏子璇 +1 位作者 王兴华 姜发纲 《华中科技大学学报(医学版)》 CAS CSCD 北大核心 2024年第3期414-419,共6页
遗传性视网膜疾病是多种先天性视网膜神经退行性疾病的总称,临床上以夜盲、进行性视野缩小、视力下降甚至失明为特点,具有多种遗传形式。由于其病变的根源在于基因突变,通过基因治疗,即利用外源性核苷酸替换或沉默基因缺陷细胞内的致病... 遗传性视网膜疾病是多种先天性视网膜神经退行性疾病的总称,临床上以夜盲、进行性视野缩小、视力下降甚至失明为特点,具有多种遗传形式。由于其病变的根源在于基因突变,通过基因治疗,即利用外源性核苷酸替换或沉默基因缺陷细胞内的致病基因,使细胞表达正确的蛋白质,恢复细胞的功能,就有可能治愈疾病。同时,眼睛具有免疫“豁免”特性,是实现基因治疗的理想器官。为了完成遗传物质的修正,治疗性核苷酸需要进入细胞内发挥作用,携带健康基因的载体递送系统是实现这一过程的有利工具。该文重点总结了包括病毒载体和非病毒载体在内的遗传性视网膜疾病基因治疗递送系统的研究进展及面临的问题。 展开更多
关键词 遗传性视网膜疾病 视网膜基因治疗 基因递送系统 病毒载体 非病毒载体
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细菌外膜囊泡应用于肿瘤治疗
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作者 王云锋 庄婉茹 +2 位作者 马宪彬 聂伟东 谢海燕 《生物化学与生物物理进展》 SCIE CAS CSCD 北大核心 2024年第2期309-327,共19页
细菌外膜囊泡(outer membrane vesicles,OMVs)是由革兰氏阴性菌分泌的纳米囊泡,主要由细菌外膜和周质成分组成,因此表面富集的病原体相关分子模式(PAMPs)使OMVs能激起强烈的免疫反应。在抗肿瘤研究中,OMVs主要被用于抗肿瘤药物的递送,... 细菌外膜囊泡(outer membrane vesicles,OMVs)是由革兰氏阴性菌分泌的纳米囊泡,主要由细菌外膜和周质成分组成,因此表面富集的病原体相关分子模式(PAMPs)使OMVs能激起强烈的免疫反应。在抗肿瘤研究中,OMVs主要被用于抗肿瘤药物的递送,不仅能增加药物的肿瘤富集还能激活免疫反应协同杀伤肿瘤;同时,OMVs也用于开发肿瘤疫苗的佐剂,可显著提高免疫响应的能力。本综述主要概括了OMVs的生物发生机理、OMVs对宿主免疫系统的影响及其在肿瘤治疗中的研究进展。 展开更多
关键词 细菌外膜囊泡 肿瘤治疗 递送载体 肿瘤疫苗
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纳米粒子在骨组织工程化基因修饰治疗中的应用
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作者 李光照 裴锡波 王剑 《中国组织工程研究》 CAS 北大核心 2025年第22期4771-4783,共13页
背景:传统的骨组织工程技术治疗临界骨缺损存在成骨效率低、安全性差等问题。而以非病毒纳米粒子为基因载体构建的基因强化型骨组织工程移植物,具有更高的成骨效率和安全性,引起了国内外学者的广泛关注和研究。目的:对当前国内外有关纳... 背景:传统的骨组织工程技术治疗临界骨缺损存在成骨效率低、安全性差等问题。而以非病毒纳米粒子为基因载体构建的基因强化型骨组织工程移植物,具有更高的成骨效率和安全性,引起了国内外学者的广泛关注和研究。目的:对当前国内外有关纳米粒子在组织工程成骨基因治疗研究中取得的新技术、新方法以及面临的挑战等进行综述,旨在为纳米粒子介导的骨组织工程基因治疗研究提供参考。方法:第一作者在Pub Med、Web of Science和中国知网数据库上进行文献检索,并以“Bone defect repair,Bone tissue engineering,Gene delivery,Nanoparticles,Non-viral gene vector,Sustained release technology,Sequential release,Targeted delivery”作为英文检索词,以“骨缺损修复,骨组织工程,基因递送,纳米粒子,非病毒基因载体,缓释技术,序贯释放,靶向递送”作为中文检索词,最终纳入84篇文献进行总结。结果与结论:(1)在骨缺损愈合的各个生理阶段进行针对性的基因递送可以显著增强骨修复效果。在早期炎症阶段,通过纳米粒子递送抗炎基因来调节炎症反应,可以为后续骨愈合奠定基础;在血管新生期,向局部递送促血管化基因有助于形成高度组织化、可灌注的血管系统,加快骨愈合速度;随着血管化的进行,骨骼的神经再支配也开始发生,此时递送促神经再生的功能性基因有利于促进神经化骨再生;在成骨阶段,通过构建纳米粒子-成骨基因复合物,可以直接提升支架及体内新骨形成的效率。(2)各种有机、无机纳米颗粒、金属有机框架和外泌体等非病毒纳米载体,在骨组织工程基因治疗中具有巨大的潜力,这些纳米基因载体各有其独特的优势和不足,因此在实际应用时,需要根据基因转染效率、生物安全性和成骨特性等因素选择最合适的类型。(3)为了全面提升递送基因的效果,目前主要通过对纳米载体进行各种功能设计来增强基因转染效率,包括增强缓释性和多基因递送序贯性等时间调控能力、增强对骨组织和成骨相关细胞的空间靶向能力、增强跨膜运输效率和细胞核靶向能力等全过程调控手段。(4)未来要进一步推动纳米粒子介导的骨组织工程基因治疗在临床上的应用,还需要克服诸多技术挑战,包括提高有机纳米基因载体的基因转染效率、降低无机纳米载体的生物安全性风险、优化新型纳米载体的生产工艺以及促进其它生理过程与成骨交互作用等,这些问题也是未来骨组织工程基因治疗的研究热点和潮流。 展开更多
关键词 骨缺损修复 骨组织工程 基因递送 纳米粒子 非病毒基因载体 缓释技术 序贯释放 靶向性递送
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考虑运输损耗和运输成本的多状态配送网络可靠性评估
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作者 徐秀珍 张媛媛 +2 位作者 何灿 岳名扬 牛义锋 《科学技术与工程》 北大核心 2024年第12期4834-4842,共9页
在考虑运输损耗和运输成本约束情形下,提出多状态配送网络可靠性评估方法。通过引入损耗因子刻画运输损耗,进而根据需求流量和运输损耗建立流分配策略;基于此分配策略、运输边容量约束以及运输成本约束,提出计算配送网络可靠性的极小容... 在考虑运输损耗和运输成本约束情形下,提出多状态配送网络可靠性评估方法。通过引入损耗因子刻画运输损耗,进而根据需求流量和运输损耗建立流分配策略;基于此分配策略、运输边容量约束以及运输成本约束,提出计算配送网络可靠性的极小容量向量方法。通过实例分析验证了算法的有效性,并从可靠性视角探讨了关键运输边的识别方法,研究成果可为管理者在配送网络运维方面提供决策指导。 展开更多
关键词 可靠性 多状态配送网络 运输损耗 运输成本 极小容量向量
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MCM-48纳米颗粒作为小核酸载体的性能研究
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作者 叶凌涛 王羽 +4 位作者 李育霖 杨迪 刘佳滢 魏芳桐 孙艳 《黑龙江八一农垦大学学报》 2024年第5期112-118,136,共8页
合成制备了MCM-48型的介孔纳米颗粒,对其进行了系统的物化表征,表明其是具有高比表面积、高度有序结构和三维特征孔道特点的纳米颗粒。对MCM-48纳米颗粒进行氨基化修饰,得到N-MCM-48纳米颗粒,研究了其作为小核酸载体的性能。结果显示:N-... 合成制备了MCM-48型的介孔纳米颗粒,对其进行了系统的物化表征,表明其是具有高比表面积、高度有序结构和三维特征孔道特点的纳米颗粒。对MCM-48纳米颗粒进行氨基化修饰,得到N-MCM-48纳米颗粒,研究了其作为小核酸载体的性能。结果显示:N-MCM-48纳米颗粒具有良好的吸附和释放小核酸的能力、细胞摄入能力和生物相容性,并能够负载siRNA进入HepG2细胞并诱导肿瘤细胞凋亡。 展开更多
关键词 MCM-48 介孔纳米颗粒 小核酸载体
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Design, mechanism, delivery and therapeutics of canonical and Dicer-substrate siRNA 被引量:2
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作者 Maria Abdul Ghafoor Raja Haliza Katas Muhammad Wahab Amjad 《Asian Journal of Pharmaceutical Sciences》 SCIE CAS 2019年第5期497-510,共14页
Upon the discovery of RNA interference(RNAi),canonical small interfering RNA(si RNA) has been recognized to trigger sequence-specific gene silencing. Despite the benefits of si RNAs as potential new drugs,there are ob... Upon the discovery of RNA interference(RNAi),canonical small interfering RNA(si RNA) has been recognized to trigger sequence-specific gene silencing. Despite the benefits of si RNAs as potential new drugs,there are obstacles still to be overcome,including off-target effects and immune stimulation. More recently,Dicer substrate si RNA(Dsi RNA) has been introduced as an alternative to si RNA. Similarly,it also is proving to be potent and target-specific,while rendering less immune stimulation. Dsi RNA is 25–30 nucleotides in length,and is further cleaved and processed by the Dicer enzyme. As with si RNA,it is crucial to design and develop a stable,safe,and efficient system for the delivery of Dsi RNA into the cytoplasm of targeted cells. Several polymeric nanoparticle systems have been well established to load Dsi RNA for in vitro and in vivo delivery,thereby overcoming a major hurdle in the therapeutic uses of Dsi RNA. The present review focuses on a comparison of si RNA and Dsi RNA on the basis of their design,mechanism,in vitro and in vivo delivery,and therapeutics. 展开更多
关键词 RNA interference Drug delivery system Polymeric nanoparticles GENE SILENCING GENE carrier NON-VIRAL vector
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A RGD-Containing Oligopeptide (K)_(16)GRGBSPC: A Novel Vector for Integrin-Mediated Targeted Gene Belivery
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作者 潘海涛 郑启新 +3 位作者 郭晓东 刘勇 李长文 宋玉林 《Journal of Huazhong University of Science and Technology(Medical Sciences)》 SCIE CAS 2006年第5期513-516,共4页
A 23 amino acid, bifunctional integrin-targeted synthetic oligopeptide was evaluated for ex vivo gene delivery to rabbit bone marrow stromal cells (BMSCs). Synthesis of the peptide (K)16GRGDSPC was performed on a ... A 23 amino acid, bifunctional integrin-targeted synthetic oligopeptide was evaluated for ex vivo gene delivery to rabbit bone marrow stromal cells (BMSCs). Synthesis of the peptide (K)16GRGDSPC was performed on a solid-phase batch peptide synthesizer. BMSCs were transfected with plasmid DNA coding for luciferase by (K)j6GRGDSPC and the transfection efficiency was assayed. The influences of chloroquine and polyethyleneimine on the transfection efficiency were also examined. The target specificity of (K)16GRGDSPC to mediate exogenous gene into BMSCs was analyzed using cell attachment test and gene delivery inhibition test. The results showed that the transfection efficiency of the oligopeptide vector was lower than that of Lipofectamine. But in the presence of endosomal buffer chloroquine or endosomal disrupting agent polyethyleneimine, the transfection efficiency of the vector was greatly enhanced. In addition, RGD-containing peptides inhibited BMSCs' attachment to the 96-well plates pretreated with fibronectin or vitronecfin and significantly decreased the transfection efficiency of the oligopeptide vector. These studies demonstrated that oligopeptide (K)16GRGDSPC was an ideal novel targeted non-viral gene delivery vector, which was easy to be synthesized, high efficient and low cytotoxicity. The vector could effectively deliver exogenous gene into rat BMSCs. 展开更多
关键词 targeted non-viral vector RGD-containing peptide gene delivery
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Using Bacterial Vectors for Probable Vaccines: From Molecular Mechanism to Cancer Therapy
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作者 Parnia Jabbari 《World Journal of Vaccines》 2020年第2期33-42,共10页
Anti-cancer therapies over the few decades, faced with many challenges. And bacterial vaccine vectors have shown a potential to be replaced as the cutting-edge technology for such aspects. Bacterial vaccine vectors wi... Anti-cancer therapies over the few decades, faced with many challenges. And bacterial vaccine vectors have shown a potential to be replaced as the cutting-edge technology for such aspects. Bacterial vaccine vectors with a suitable DNA can be a potential option for cancer treatment as a carrier for tumoricidal agents or bacterially directed Enzyme Prodrug treatment. Throughout this study, it is planned to have a review of the use of bacteria as vehicles by different ways for cancer treatment, detailing the systems of function and achievements at preclinical and clinical levels. 展开更多
关键词 Bacterial vector Vaccine delivery System Cancer Vaccine DNA Vaccine TUMOR
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Vectors for gene therapy:A place for DNA transposon
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作者 Benoit Chenais 《Open Journal of Genetics》 2013年第2期1-11,共11页
Gene therapy offers important perspectives in current and future medicine but suffers from imperfect vectors for the delivery of the therapeutic gene. Most preclinical and clinical trials have been based on the use of... Gene therapy offers important perspectives in current and future medicine but suffers from imperfect vectors for the delivery of the therapeutic gene. Most preclinical and clinical trials have been based on the use of viral vectors, which have evident advantages but also some serious disadvantages. In the past decade the use of DNA transposon-based systems for gene delivery has emerged as a non-viral alternative. DNA transposon vector engineering remains largely in a preclinical phase but some interesting results have been obtained. This mini-review aims to provide the current state of the art on DNA transposon vectors used in a gene therapy perspective. 展开更多
关键词 Transposable Element Gene delivery Non-Viral vectors PIGGYBAC Sleeping Beauty
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The potential of gene therapies for spinal cord injury repair:a systematic review and meta-analysis of pre-clinical studies 被引量:1
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作者 Catriona J.Cunningham Mindaugas Viskontas +5 位作者 Krzysztof Janowicz Yasmin Sani Malin EHåkansson Anastasia Heidari Wenlong Huang Xuenong Bo 《Neural Regeneration Research》 SCIE CAS CSCD 2023年第2期299-305,共7页
Currently,there is no cure for traumatic spinal co rd injury but one therapeutic approach showing promise is gene therapy.In this systematic review and meta-analysis,we aim to assess the efficacy of gene therapies in ... Currently,there is no cure for traumatic spinal co rd injury but one therapeutic approach showing promise is gene therapy.In this systematic review and meta-analysis,we aim to assess the efficacy of gene therapies in pre-clinical models of spinal cord injury and the risk of bias.In this metaanalysis,registe red at PROSPERO(Registration ID:CRD42020185008),we identified relevant controlled in vivo studies published in English by searching the PubMed,Web of Science,and Embase databases.No restrictions of the year of publication were applied and the last literature search was conducted on August 3,2020.We then conducted a random-effects meta-analysis using the restricted maximum likelihood estimator.A total of 71 studies met our inclusion crite ria and were included in the systematic review.Our results showed that overall,gene therapies were associated with improvements in locomotor score(standardized mean difference[SMD]:2.07,95%confidence interval[CI]:1.68-2.47,Tau^(2)=2.13,I^(2)=83.6%)and axonal regrowth(SMD:2.78,95%CI:1.92-3.65,Tau^(2)=4.13,I^(2)=85.5%).There was significant asymmetry in the funnel plots of both outcome measures indicating the presence of publication bias.We used a modified CAMARADES(Collaborative Approach to M eta-Analysis and Review of Animal Data in Experimental Studies)checklist to assess the risk of bias,finding that the median score was 4(IQR:3-5).In particula r,reports of allocation concealment and sample size calculations were lacking.In conclusion,gene therapies are showing promise as therapies for spinal co rd injury repair,but there is no consensus on which gene or genes should be targeted. 展开更多
关键词 animal models gene delivery META-ANALYSIS regenerative medicine spinal cord injury systematic review viral vectors
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遗传性听力障碍的基因治疗研究进展
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作者 令娜娜 郭玉芬 徐百成 《中国听力语言康复科学杂志》 2023年第4期394-398,共5页
听力障碍是常见的感觉障碍性疾病之一,目前用于治疗遗传性听力障碍的临床选择有限,主要是通过配戴助听器或植入人工耳蜗等补偿或重建听力,并不能恢复自然听力。从病理学来说,恢复生理听觉是听力障碍治疗的必然目标,因此基因治疗才是听... 听力障碍是常见的感觉障碍性疾病之一,目前用于治疗遗传性听力障碍的临床选择有限,主要是通过配戴助听器或植入人工耳蜗等补偿或重建听力,并不能恢复自然听力。从病理学来说,恢复生理听觉是听力障碍治疗的必然目标,因此基因治疗才是听力障碍康复的最终方法。目前遗传性听力障碍的基因治疗还处在动物实验阶段,如何强化该基础研究并促进其向临床转化,是未来研究的重点。本文对遗传性听力障碍基因治疗的现状、发展前景和挑战等进行文献复习和发展展望。 展开更多
关键词 遗传性听力障碍 基因治疗 靶细胞 基因载体 载体导入途径 基因置换 基因沉默 基因编辑
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CRISPR/Cas9基因编辑非病毒递送系统
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作者 盛劲菡 郑琪臻 汪铭 《高等学校化学学报》 SCIE EI CAS CSCD 北大核心 2023年第3期156-166,共11页
规律间隔成簇短回文重复序列及相关蛋白9(CRISPR/Cas9)系统的基因编辑技术为哺乳细胞基因组的精准修饰与编辑研究提供了高效、快捷的工具,但其化学生物学应用依然面临着CRISPR基因编辑工具Cas9蛋白和gRNA的细胞及活体递送等问题.近年来... 规律间隔成簇短回文重复序列及相关蛋白9(CRISPR/Cas9)系统的基因编辑技术为哺乳细胞基因组的精准修饰与编辑研究提供了高效、快捷的工具,但其化学生物学应用依然面临着CRISPR基因编辑工具Cas9蛋白和gRNA的细胞及活体递送等问题.近年来,研究人员通过开发多种非病毒递送载体,实现了编码CRISPR/Cas9基因编辑工具的DNA和信使RNA(mRNA)以及Cas9/gRNA核糖核蛋白(RNP)复合物的递送,并应用于靶基因的化学修饰与编辑调控.本文主要概述了近期CRISPR/Cas9基因编辑递送的研究进展,并对其化学生物学应用前景进行了展望. 展开更多
关键词 规律间隔成簇短回文重复序列及相关蛋白9(CRISPR/Cas9) 基因编辑 药物递送 非病毒载体 纳米颗粒
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基于纳豆激酶特性的功能递送载体研究进展
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作者 解明浩 徐献兵 +2 位作者 王震宇 程述震 杜明 《食品科学》 EI CAS CSCD 北大核心 2023年第17期153-161,共9页
纳豆激酶是一种具有溶栓功能的丝氨酸蛋白酶,与临床溶栓药物(尿激酶和链激酶)相比,其具有价格低、效率高、可口服、无毒副作用等优点,是一种理想的预防和治疗血栓疾病的天然活性物质。基于纳豆激酶天然生物酶学性质,目前市场产品存在活... 纳豆激酶是一种具有溶栓功能的丝氨酸蛋白酶,与临床溶栓药物(尿激酶和链激酶)相比,其具有价格低、效率高、可口服、无毒副作用等优点,是一种理想的预防和治疗血栓疾病的天然活性物质。基于纳豆激酶天然生物酶学性质,目前市场产品存在活性和生物利用度低等问题。现有纳豆激酶产品经口腔-胃-肠消化吸收系统后,进入血液发挥溶栓功能,而消化吸收系统环境(低pH值等)和因子(胃蛋白酶、胰蛋白酶等)会破坏纳豆激酶空间结构,导致其溶栓功能活性降低或丧失。利用脂质、多糖、蛋白质等天然生物材料构建纳豆激酶功能递送载体,可有效规避消化系统中的不利因素,增强纳豆激酶稳定性,提高其在机体内的生物活性和利用度。本文基于纳豆激酶的结构和功能特性,综述了纳豆激酶功能递送载体的研究进展,以期为高效、稳定型纳豆激酶溶栓产品的开发提供理论参考和技术支持。 展开更多
关键词 纳豆激酶 包埋 功能递送载体 抗血栓
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将目标物质靶向至心脏的研究进展
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作者 宋紫微 陈鹏莉(综述) +1 位作者 张曼玉 李丽丽(审校) 《微循环学杂志》 2023年第2期90-94,共5页
当前心血管疾病的发病率和死亡率仍位居全球前列。随着分子生物学和细胞心脏病学知识的不断更新,基因治疗已在临床研究中有了长足的进步。然而对于如何将目的细胞或基因成功运送到心脏并发挥作用,仍需克服各种障碍,如解剖、血流机械力... 当前心血管疾病的发病率和死亡率仍位居全球前列。随着分子生物学和细胞心脏病学知识的不断更新,基因治疗已在临床研究中有了长足的进步。然而对于如何将目的细胞或基因成功运送到心脏并发挥作用,仍需克服各种障碍,如解剖、血流机械力、内皮屏障、细胞屏障以及免疫应答等。病毒和非病毒载体,一些细胞及细胞外囊泡、生物材料、外科手术等方法已被广泛运用于各种临床前实验当中。 展开更多
关键词 基因治疗 生物载体 病毒载体 传递障碍
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纳米生物技术基因治疗载体研究进展 被引量:7
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作者 张小伟 田聆 魏于全 《生物医学工程学杂志》 EI CAS CSCD 北大核心 2005年第3期610-613,共4页
基因治疗已在治疗多种人类重大疾病如遗传病、肿瘤等方面显示出良好的应用前景,但也面临着巨大的挑战,其中之一就是需要安全、高效、靶向的载体系统。纳米生物材料,如脂质体、聚丙交酯-乙交酯(PLGA),聚乳酸(PLA)等,由于具有良好的生物... 基因治疗已在治疗多种人类重大疾病如遗传病、肿瘤等方面显示出良好的应用前景,但也面临着巨大的挑战,其中之一就是需要安全、高效、靶向的载体系统。纳米生物材料,如脂质体、聚丙交酯-乙交酯(PLGA),聚乳酸(PLA)等,由于具有良好的生物安全性、可方便有效地实现基因靶向性及高效表达和缓释,成为制备高效、靶向的基因治疗载体系统的良好介质,日益在基因治疗载体系统中受到广泛重视。本文综述了目前在基因治疗领域中常用的纳米载体的生物学特性,以及它们的最新研究进展。 展开更多
关键词 纳米生物技术 基因治疗 载体 生物材料 纳米粒 壳聚糖
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应用PAMAM dendrimers作为DNA运送载体的体外研究 被引量:5
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作者 郭晨莹 王恒 +1 位作者 蔺亚晖 蔡启良 《生物化学与生物物理进展》 SCIE CAS CSCD 北大核心 2004年第9期804-811,共8页
StarburstTMPAMAMdendrimers分子是一类新型的高分枝、辐射状对称的树状高分子,在生理条件下其表面具有高密度的正电荷,可以通过静电相互作用与核酸形成复合物后,介导遗传物质进入细胞.研究了G3,G3 5,G5,G7,G7 5,G9各代dendrimers分子与... StarburstTMPAMAMdendrimers分子是一类新型的高分枝、辐射状对称的树状高分子,在生理条件下其表面具有高密度的正电荷,可以通过静电相互作用与核酸形成复合物后,介导遗传物质进入细胞.研究了G3,G3 5,G5,G7,G7 5,G9各代dendrimers分子与DNA结合后介导其转染细胞的能力,并初步评价这种复合物转染对细胞活力的影响.实验证实,全代的PAMAMdendrmers皆可与DNA结合,并可在体外培养的细胞中介导高效的DNA转染.PAMAMdendrimer/DNA复合物很稳定,在较大的pH值变化范围内(pH 2~ 10 )不解离.PAMAMdendrimers可保护与之复合的DNA分子免受限制性内切酶的降解.在一定的电荷比范围内,高代数的dendrimers分子与DNA形成的复合物对培养细胞的转染效率高于低代数dendrimer分子,复合物所介导的转染效率在不同的细胞系之间也有差异.在有效作用浓度范围内(≤ 1 3× 10 -1g/L),PAMAMdendrimers/DNA复合物对被转染细胞无毒性.但是,未与DNA复合的dendrimers分子在较低浓度时则表现出毒性,表明StarburstTM PAMAMdendrimers分子可作为新型的低毒非病毒DNA载体,用于介导DNA对体外培养细胞的转染.这些前期观察。 展开更多
关键词 DNA转运 Starburst^TM PAMAM DENDRIMERS 转染 非病毒载体
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