Synapses are essential units for the flow of information in the brain.Over the last 70 years,synapses have been widely studied in multiple animal models including worms,fruit flies,and rodents.In comparison,the study ...Synapses are essential units for the flow of information in the brain.Over the last 70 years,synapses have been widely studied in multiple animal models including worms,fruit flies,and rodents.In comparison,the study of human synapses has evolved significantly slower,mainly because of technical limitations.However,three novel methods allowing the analysis of molecular,morphological,and functional properties of human synapses may expand our knowledge of the human brain.Here,we briefly describe these methods,and evaluate how the information provided by each unique approach may contribute to the functional and anatomical analysis of the synaptic component of human brain circuitries.In particular,using tissue from cryopreserved human brains,synaptic plasticity can be studied in isolated synaptosomes by fluorescence analysis of single-synapse long-term potentiation（FASS-LTP）,and subpopulations of synapses can be thoroughly assessed in the ribbons of brain tissue by array tomography（AT）.Currently,it is also possible to quantify synaptic density in the living human brain by positron emission tomography（PET）,using a novel synaptic radio-ligand.Overall,data provided by FASS-LTP,AT,and PET may significantly contribute to the global understanding of synaptic structure and function in both healthy and diseased human brains,thus directly impacting translational research.展开更多
The rise of tidal level in tidal reaches induced by sea-level rise has a large impact on flood control and water supply for the regions around the estuary. This paper focuses on the variations of tidal level response ...The rise of tidal level in tidal reaches induced by sea-level rise has a large impact on flood control and water supply for the regions around the estuary. This paper focuses on the variations of tidal level response along the tidal reaches in the Yangtze Estuary, as well as the impacts of upstream discharge on tidal level response, due to the sea-level rise of the East China Sea. Based on the Topex/Poseidon altimeter data obtained during the period 1993-2005, a stochastic dynamic analysis was performed and a forecast model was run to predict the sea-level rise of the East China Sea. Two- dimensional hydrodynamic numerical models downscaling from the East China Sea to estuarine areas were implemented to analyze the rise of tidal level along the tidal reaches. In response to the sea-level rise, the tidal wave characteristics change slightly in nearshore areas outside the estuaries, involving the tidal range and the duration of flood and ebb tide. The results show that the rise of tidal level in the tidal reaches due to the sea-level rise has upstream decreasing trends. The step between the stations of Zhangjiagang and Shiyiwei divides the tidal reaches into two parts, in which the tidal level response declines slightly. The rise of tidal level is 1-2.5 mm/a in the upper part, and 4-6 mm/a in the lower part. The stations of Jiangyin and Yanglin, as an example of the upper part and the lower part respectively, are extracted to analyze the impacts of upstream discharge on tidal level response to the sea-level rise. The relation between the rise of tidal level and the upstream discharge can be fitted well with a quadratic fimction in the upper part. However, the relation is too complicated to be fitted in the lower part because of the tide dominance. For comparison purposes, hourly tidal level observations at the stations of Xuliujing and Yanglin during the period 1993-2009 are adopted. In order to uniform the influence of upstream discharge on tidal level for a certain day each year, the hourly tidal level observations are corrected by the correlation between the increment of tidal level and the increment of daily mean upstream discharge. The rise of annual mean tidal level is evaluated. The resulting rise of tidal level at the stations of Xuliujing and Yanglin is 3.0 mm/a and 6.6 mm/a respectively, close to the rise of 5 mm/a according to the proposed relation between the rise of tidal level and the upstream discharge.展开更多
A bacterium strain B26 capable of producing(R)-α-hydroxyphenylacetic acid [(R)-HPA](yield,47.5%;enantiomeric excess,99.1%) from phenylglyoxylic acid(PGA) with high optical purity was isolated and identified as Bacill...A bacterium strain B26 capable of producing(R)-α-hydroxyphenylacetic acid [(R)-HPA](yield,47.5%;enantiomeric excess,99.1%) from phenylglyoxylic acid(PGA) with high optical purity was isolated and identified as Bacillus sp.B26 by 16S rDNA(ribosomal DNA) sequencing.Phylogenic analysis showed that the strain was most similar to Bacillus sp.enrichment culture clone SYW5(FJ601635.1) and Bacillus cereus strain FM-4(EU794727.1).Efforts were made to further improve HPA-production and PGA-tolerance by UV irradiation and UV-LiCl cooperative mutagenesis.Among viable mutants,B.sp.UV-38 and B.sp.ULi-11 exhibited better productivities than the wild type.Comparisons of HPA production and time course among wild strain and two mutants showed that B.sp.ULi-11 was more competent than B.sp.UV-38.HPA production was increased by 39.1% with B.sp.ULi-11(yield,65.4%) compared to that with B.sp.B26(yield,47.0%) when cultured in fermentation broth(pH 7.2) at 32℃ with an agitation speed of 180 r·min-1 and PGA final concentration of 15 mmol·L-1 for 25 h.展开更多
A new halogenated biindole and a new apo-carotenone have been isolated from the ethanolic extract of the green alga Chaetomorpha basiretorsa Sethcell. On the basis of chemical and spectroscopic methods including 2D NM...A new halogenated biindole and a new apo-carotenone have been isolated from the ethanolic extract of the green alga Chaetomorpha basiretorsa Sethcell. On the basis of chemical and spectroscopic methods including 2D NMR technique, their structures have been elucidated as 4,4′-dichloro-5,5′-dibromo-7,7′-dimethoxy-2,2′-bi-1H-indole and 1′S*,4′R*-8-(4′-hydroxy-2′,6′,6′- trimethylcyclohex-2-enyl)-6-methyloct-3E,5E,7E-trien-2-one, respectively.展开更多
AIM To investigate the antitumor effects and underlying mechanisms of(17 R,18 R)-2-(1-hexyloxyethyl)-2-devinyl chlorine E6 trisodium salt(YLG-1)-induced photodynamic therapy(PDT) on pancreatic cancer in vitro and in v...AIM To investigate the antitumor effects and underlying mechanisms of(17 R,18 R)-2-(1-hexyloxyethyl)-2-devinyl chlorine E6 trisodium salt(YLG-1)-induced photodynamic therapy(PDT) on pancreatic cancer in vitro and in vivo.METHODS YLG-1 is a novel photosensitizer extracted from spirulina. Its phototoxicity, cellular uptake and localization, as well as its effect on reactive oxygen species(ROS) production, apoptosis, and expression of apoptosis-associated proteins were detected in vitro. An in vivo imaging system(IVIS), the Lumina K imaging system, and mouse models of subcutaneous Panc-1-bearing tumors were exploited to evaluate the drug delivery pathway and pancreatic cancer growth in vivo.RESULTS YLG-1 was localized to the mitochondria, and the appropriate incubation time was 6 h. Under 650 nm light irradiation, YLG-1-PDT exerted a potent cytotoxic effect on pancreatic cancer cells in vitro, which could be abolished by the ROS scavenger N-acetyl-L-cysteine(NAC). The death mode caused by YLG-1-PDT was apoptosis, accompanied by upregulated Bax and cleaved Caspase-3 and decreased Bcl-2 expression. The results from the IVIS images suggested that the optimal administration route was intratumoral(IT) injection and that the best time to conduct YLG-1-PDT was 2 h post-IT injection. Consistent with the results in vitro, YLG-1-PDT showed great growth inhibition effects on pancreatic cancer cells in a mouse model.CONCLUSION YLG-1 is a potential photosensitizer for pancreatic cancer PDT via IT injection, the mechanisms of which are associated with inducing ROS and promoting apoptosis.展开更多
With the limited production and use of R245fa,environmentally friendly refrigerant has attracted the attention of researchers.Due to the similar thermal characteristics,R1233zd(E)is considered to be an ideal substitut...With the limited production and use of R245fa,environmentally friendly refrigerant has attracted the attention of researchers.Due to the similar thermal characteristics,R1233zd(E)is considered to be an ideal substitute for R245fa in heat pump systems.In this study,the performance and economic analysis of heat pump systems with R245fa and R1233zd(E)as refrigerants are carried out.The results show that the total cost of R1233zd(E)system is more than 10%higher than that of R245fa system under the same heating load.With the increase of condensation temperature,the heating capacity of both systems decreases,and with the increase of evaporation temperature,the heating capacity increases.The variation trend of coefficient of performance(COP)of the two systems is similar to that of heating capacity.Under the same operating conditions,the COP of R1233zd(E)system is 19.2%higher than that of R245fa system,and the volumetric heat capacity of R1233zd(E)is 9.0%–13.9%lower than that of R245fa.The economic analysis results show that the investment cost of R1233zd(E)system is low under the same heat load.展开更多
Started from 5-hydroxy-2-pentanone, (R)(E)-3,7-dimethyl-2-octene-1,8-dioic acid, callosobruchusic acid, was synthesized via five steps with D-(-)-camphor sultam as the chiral auxiliary. It was of good optical purity a...Started from 5-hydroxy-2-pentanone, (R)(E)-3,7-dimethyl-2-octene-1,8-dioic acid, callosobruchusic acid, was synthesized via five steps with D-(-)-camphor sultam as the chiral auxiliary. It was of good optical purity and yield.展开更多
基金supported by National Institutes of Health Grants R21-AG048506,P01-AG000538 and RO1-AG34667(to CWC)UC MEXUS-CONACYT Grant CN-16-170(to GAP and CWC)
文摘Synapses are essential units for the flow of information in the brain.Over the last 70 years,synapses have been widely studied in multiple animal models including worms,fruit flies,and rodents.In comparison,the study of human synapses has evolved significantly slower,mainly because of technical limitations.However,three novel methods allowing the analysis of molecular,morphological,and functional properties of human synapses may expand our knowledge of the human brain.Here,we briefly describe these methods,and evaluate how the information provided by each unique approach may contribute to the functional and anatomical analysis of the synaptic component of human brain circuitries.In particular,using tissue from cryopreserved human brains,synaptic plasticity can be studied in isolated synaptosomes by fluorescence analysis of single-synapse long-term potentiation（FASS-LTP）,and subpopulations of synapses can be thoroughly assessed in the ribbons of brain tissue by array tomography（AT）.Currently,it is also possible to quantify synaptic density in the living human brain by positron emission tomography（PET）,using a novel synaptic radio-ligand.Overall,data provided by FASS-LTP,AT,and PET may significantly contribute to the global understanding of synaptic structure and function in both healthy and diseased human brains,thus directly impacting translational research.
基金supported by the State Key Development Program of Basic Research of China (Grant No. 2010CB429001)the Special Fund of State Key Laboratory of Hydrology-Water Resources and Hydraulic Engineering (Grant No. 2009586812)the Priority Academic Program Development of Jiangsu Higher Education Institutions (Coastal Development Conservancy) (PAPD)
文摘The rise of tidal level in tidal reaches induced by sea-level rise has a large impact on flood control and water supply for the regions around the estuary. This paper focuses on the variations of tidal level response along the tidal reaches in the Yangtze Estuary, as well as the impacts of upstream discharge on tidal level response, due to the sea-level rise of the East China Sea. Based on the Topex/Poseidon altimeter data obtained during the period 1993-2005, a stochastic dynamic analysis was performed and a forecast model was run to predict the sea-level rise of the East China Sea. Two- dimensional hydrodynamic numerical models downscaling from the East China Sea to estuarine areas were implemented to analyze the rise of tidal level along the tidal reaches. In response to the sea-level rise, the tidal wave characteristics change slightly in nearshore areas outside the estuaries, involving the tidal range and the duration of flood and ebb tide. The results show that the rise of tidal level in the tidal reaches due to the sea-level rise has upstream decreasing trends. The step between the stations of Zhangjiagang and Shiyiwei divides the tidal reaches into two parts, in which the tidal level response declines slightly. The rise of tidal level is 1-2.5 mm/a in the upper part, and 4-6 mm/a in the lower part. The stations of Jiangyin and Yanglin, as an example of the upper part and the lower part respectively, are extracted to analyze the impacts of upstream discharge on tidal level response to the sea-level rise. The relation between the rise of tidal level and the upstream discharge can be fitted well with a quadratic fimction in the upper part. However, the relation is too complicated to be fitted in the lower part because of the tide dominance. For comparison purposes, hourly tidal level observations at the stations of Xuliujing and Yanglin during the period 1993-2009 are adopted. In order to uniform the influence of upstream discharge on tidal level for a certain day each year, the hourly tidal level observations are corrected by the correlation between the increment of tidal level and the increment of daily mean upstream discharge. The rise of annual mean tidal level is evaluated. The resulting rise of tidal level at the stations of Xuliujing and Yanglin is 3.0 mm/a and 6.6 mm/a respectively, close to the rise of 5 mm/a according to the proposed relation between the rise of tidal level and the upstream discharge.
基金Supported by the Specialized Research Fund for the Doctoral Program of Higher Education of China (20103514110002)the Science and Technology Key Project of Fujian Province (2009N0046)
文摘A bacterium strain B26 capable of producing(R)-α-hydroxyphenylacetic acid [(R)-HPA](yield,47.5%;enantiomeric excess,99.1%) from phenylglyoxylic acid(PGA) with high optical purity was isolated and identified as Bacillus sp.B26 by 16S rDNA(ribosomal DNA) sequencing.Phylogenic analysis showed that the strain was most similar to Bacillus sp.enrichment culture clone SYW5(FJ601635.1) and Bacillus cereus strain FM-4(EU794727.1).Efforts were made to further improve HPA-production and PGA-tolerance by UV irradiation and UV-LiCl cooperative mutagenesis.Among viable mutants,B.sp.UV-38 and B.sp.ULi-11 exhibited better productivities than the wild type.Comparisons of HPA production and time course among wild strain and two mutants showed that B.sp.ULi-11 was more competent than B.sp.UV-38.HPA production was increased by 39.1% with B.sp.ULi-11(yield,65.4%) compared to that with B.sp.B26(yield,47.0%) when cultured in fermentation broth(pH 7.2) at 32℃ with an agitation speed of 180 r·min-1 and PGA final concentration of 15 mmol·L-1 for 25 h.
基金supported by national 863 project(Grant No.2004AA625030,2001AA620503)NNSFC(Grant No.20432030)Key Innovative Project of the Academy(Grant No.KZCX3-SW-215).
文摘A new halogenated biindole and a new apo-carotenone have been isolated from the ethanolic extract of the green alga Chaetomorpha basiretorsa Sethcell. On the basis of chemical and spectroscopic methods including 2D NMR technique, their structures have been elucidated as 4,4′-dichloro-5,5′-dibromo-7,7′-dimethoxy-2,2′-bi-1H-indole and 1′S*,4′R*-8-(4′-hydroxy-2′,6′,6′- trimethylcyclohex-2-enyl)-6-methyloct-3E,5E,7E-trien-2-one, respectively.
基金Supported by National Natural Science Foundation of China,No.81472844
文摘AIM To investigate the antitumor effects and underlying mechanisms of(17 R,18 R)-2-(1-hexyloxyethyl)-2-devinyl chlorine E6 trisodium salt(YLG-1)-induced photodynamic therapy(PDT) on pancreatic cancer in vitro and in vivo.METHODS YLG-1 is a novel photosensitizer extracted from spirulina. Its phototoxicity, cellular uptake and localization, as well as its effect on reactive oxygen species(ROS) production, apoptosis, and expression of apoptosis-associated proteins were detected in vitro. An in vivo imaging system(IVIS), the Lumina K imaging system, and mouse models of subcutaneous Panc-1-bearing tumors were exploited to evaluate the drug delivery pathway and pancreatic cancer growth in vivo.RESULTS YLG-1 was localized to the mitochondria, and the appropriate incubation time was 6 h. Under 650 nm light irradiation, YLG-1-PDT exerted a potent cytotoxic effect on pancreatic cancer cells in vitro, which could be abolished by the ROS scavenger N-acetyl-L-cysteine(NAC). The death mode caused by YLG-1-PDT was apoptosis, accompanied by upregulated Bax and cleaved Caspase-3 and decreased Bcl-2 expression. The results from the IVIS images suggested that the optimal administration route was intratumoral(IT) injection and that the best time to conduct YLG-1-PDT was 2 h post-IT injection. Consistent with the results in vitro, YLG-1-PDT showed great growth inhibition effects on pancreatic cancer cells in a mouse model.CONCLUSION YLG-1 is a potential photosensitizer for pancreatic cancer PDT via IT injection, the mechanisms of which are associated with inducing ROS and promoting apoptosis.
基金supported by theKorea Institute of Energy Technology Evaluationand Planning (KETEP) grant funded by the Korean Government (MOTIE) (No. 20202020900060,The development and application of operational technology in smart farm utilizing waste heat fromparticulates reduced smokestack).
文摘With the limited production and use of R245fa,environmentally friendly refrigerant has attracted the attention of researchers.Due to the similar thermal characteristics,R1233zd(E)is considered to be an ideal substitute for R245fa in heat pump systems.In this study,the performance and economic analysis of heat pump systems with R245fa and R1233zd(E)as refrigerants are carried out.The results show that the total cost of R1233zd(E)system is more than 10%higher than that of R245fa system under the same heating load.With the increase of condensation temperature,the heating capacity of both systems decreases,and with the increase of evaporation temperature,the heating capacity increases.The variation trend of coefficient of performance(COP)of the two systems is similar to that of heating capacity.Under the same operating conditions,the COP of R1233zd(E)system is 19.2%higher than that of R245fa system,and the volumetric heat capacity of R1233zd(E)is 9.0%–13.9%lower than that of R245fa.The economic analysis results show that the investment cost of R1233zd(E)system is low under the same heat load.
基金Supported by the National Natural Science Foundation of China( No. 2 9872 0 12)
文摘Started from 5-hydroxy-2-pentanone, (R)(E)-3,7-dimethyl-2-octene-1,8-dioic acid, callosobruchusic acid, was synthesized via five steps with D-(-)-camphor sultam as the chiral auxiliary. It was of good optical purity and yield.
