目的:建立大鼠血浆中刺五加苷 E 和刺五加苷 B 的 RP-HPLC 分析方法,并对这2种成分在大鼠体内过程特性进行分析研究。方法:用乙腈沉淀生物样品中的蛋白质,同时又将生物样品中的刺五加苷 B 和苷 E 提取出来,然后用固相萃取法分离提取物,...目的:建立大鼠血浆中刺五加苷 E 和刺五加苷 B 的 RP-HPLC 分析方法,并对这2种成分在大鼠体内过程特性进行分析研究。方法:用乙腈沉淀生物样品中的蛋白质,同时又将生物样品中的刺五加苷 B 和苷 E 提取出来,然后用固相萃取法分离提取物,用60%甲醇将刺五加苷 B 和苷 E 从固相萃取小柱洗脱下来,用高效液相色谱法提取出来测定,色谱柱为 KromasilODS(4.6mm×250mm,5pan),柱温25℃,水-乙腈梯度流动相(0→15min,90:10→80:20;15→25min,80:20→50:50),流速0.8mL·min^(-1),一次进样,分别在220nm和206nm 波长下检测刺五加苷 E 和苷 B。结果:Wister 大鼠一次股静脉给药后血药浓度时间曲线呈三室模型,刺五加苷 E 和苷 B 的消除半衰期 t_(1/2)分别为4.66和2.49h。在主要组织中的分布特点是:刺五加苷 E 在血液、肾脏、心脏、肝脏和脾脏中都有分布,刺五加苷 B 在血液、肾脏、心脏、肝脏中都有分布,在脾脏中没有分布,刺五加苷 E 和苷 B 主要由肝、肾代谢、排泄。结论:本法可用于刺五加苷 E 和刺五加苷 B 体内过程的研究。样品直接用固相萃取小柱处理,可消除内源性成分干扰。展开更多
The effects of radial and poloidal E×B drifts in the scrape-off layer （SOL） of a limiter tokamak are studied with a one-dimensional fluid code. The transport equations are solved in the poloidal direction with ...The effects of radial and poloidal E×B drifts in the scrape-off layer （SOL） of a limiter tokamak are studied with a one-dimensional fluid code. The transport equations are solved in the poloidal direction with the radial influxes as the source terms. The simulation results show that in the high recycling regime, the effect of the radial E×B drift on plasma density tends to be stronger than that of the poloidal E × B drift. In the sheath-limited regime, the effects of the radial E × B drift and poloidal E × B drift on plasma density are almost equally important. Considering the influence on the electron temperature, the poloidal E × B drift tends to be more important than the radial E × B drift in both the high recycling regime and sheath-limited regime. For the normal By, the poloidal E × B drift tends to raise the pressure at the low field side while the radial E × B drift favours the high field side. The simulation results also show that the E × B drift influences the asymmetries on the parameter distributions at the high field side and low field side, and the distributions are much more symmetric with the field reversal.展开更多
文摘目的:建立大鼠血浆中刺五加苷 E 和刺五加苷 B 的 RP-HPLC 分析方法,并对这2种成分在大鼠体内过程特性进行分析研究。方法:用乙腈沉淀生物样品中的蛋白质,同时又将生物样品中的刺五加苷 B 和苷 E 提取出来,然后用固相萃取法分离提取物,用60%甲醇将刺五加苷 B 和苷 E 从固相萃取小柱洗脱下来,用高效液相色谱法提取出来测定,色谱柱为 KromasilODS(4.6mm×250mm,5pan),柱温25℃,水-乙腈梯度流动相(0→15min,90:10→80:20;15→25min,80:20→50:50),流速0.8mL·min^(-1),一次进样,分别在220nm和206nm 波长下检测刺五加苷 E 和苷 B。结果:Wister 大鼠一次股静脉给药后血药浓度时间曲线呈三室模型,刺五加苷 E 和苷 B 的消除半衰期 t_(1/2)分别为4.66和2.49h。在主要组织中的分布特点是:刺五加苷 E 在血液、肾脏、心脏、肝脏和脾脏中都有分布,刺五加苷 B 在血液、肾脏、心脏、肝脏中都有分布,在脾脏中没有分布,刺五加苷 E 和苷 B 主要由肝、肾代谢、排泄。结论:本法可用于刺五加苷 E 和刺五加苷 B 体内过程的研究。样品直接用固相萃取小柱处理,可消除内源性成分干扰。
基金supported by National Natural Science Foundation of China (No. 10135020)
文摘The effects of radial and poloidal E×B drifts in the scrape-off layer （SOL） of a limiter tokamak are studied with a one-dimensional fluid code. The transport equations are solved in the poloidal direction with the radial influxes as the source terms. The simulation results show that in the high recycling regime, the effect of the radial E×B drift on plasma density tends to be stronger than that of the poloidal E × B drift. In the sheath-limited regime, the effects of the radial E × B drift and poloidal E × B drift on plasma density are almost equally important. Considering the influence on the electron temperature, the poloidal E × B drift tends to be more important than the radial E × B drift in both the high recycling regime and sheath-limited regime. For the normal By, the poloidal E × B drift tends to raise the pressure at the low field side while the radial E × B drift favours the high field side. The simulation results also show that the E × B drift influences the asymmetries on the parameter distributions at the high field side and low field side, and the distributions are much more symmetric with the field reversal.
