背景与目的细胞视黄酸结合蛋白(cellular retinoic acid-binding protein II,C BPII)和表皮型脂肪酸结合蛋白(epidermal fa y acid-binding protein,E-FABP)作为维甲酸(retinoic acid,)的转运蛋白,通过信号传导通路,从正反两方面影响细...背景与目的细胞视黄酸结合蛋白(cellular retinoic acid-binding protein II,C BPII)和表皮型脂肪酸结合蛋白(epidermal fa y acid-binding protein,E-FABP)作为维甲酸(retinoic acid,)的转运蛋白,通过信号传导通路,从正反两方面影响细胞的增殖和凋亡。本研究旨在探讨CRABPII和E-FABP在非小细胞肺癌(non-small cell lungcancer,NSCLC)中的表达及意义。方法利用组织芯片技术和免疫组织化学SP法检测54例正常肺组织、287例NSCLC原发癌组织以及103例淋巴结转移癌组织中C BPII和E-FABP的表达。结果 C BPII在NSCLC原发癌组织中的表达与患者的性别、肿瘤的有无转移、TNM分期有关(P<0.05)。E-FABP在NSCLC原发癌组织中的阳性表达率分别高于正常肺组织和淋巴结转移癌组织(P<0.05)。在NSCLC原发癌组织中,E-FABP的表达与肿瘤的病理分级、有无转移有关(P<0.05)。在NSCLC中,E-FABP的阳性表达较CRABPII占优势(P<0.05),两种蛋白的差异性表达与肿瘤的大小、病理分级、有无转移、TNM分期有关(P<0.05),瘤体愈大,肿瘤发生转移,临床分期愈晚,E-FABP的表达愈占优势。Kaplan-Meier单因素生存分析显示:C BPII的表达、C BPII与E-FABP的差异性表达与NSCLC患者的预后有关(P<0.05)。结论 E-FABP在NSCLC中高表达,其表达的增强可能与NSCLC的发生和演进有关;C BPII可能在NSCLC的发展过程中起负向调节作用,C BPII阴性表达患者术后生存率更高,对NSCLC患者预后的评估有一定价值。展开更多
Objective: To study the correlation of serum E-FABP content with glucose lipid metabolism, endothelial injury and inflammatory response in children with metabolic syndrome. Methods:A total of 49 children with metaboli...Objective: To study the correlation of serum E-FABP content with glucose lipid metabolism, endothelial injury and inflammatory response in children with metabolic syndrome. Methods:A total of 49 children with metabolic syndrome who were treated in the hospital between January 2016 and February 2017 were collected as observation group, and 50 healthy children who received vaccination in the hospital over the same period were collected as normal control group. Serum levels of E-FABP as well as glucose lipid metabolism, endothelial injury and inflammatory response indexes of two groups of subjects were detected, and the Pearson test was used to evaluate the correlation of E-FABP content with glucose lipid metabolism, endothelial injury and inflammatory response. Results: Serum E-FABP content of observation group was higher than that of control group;glucose metabolism indexes FBG and HOMA-IR levels in peripheral blood were higher than those of normal control group;lipid metabolism indexes TC, LDL-C and ApB contents in peripheral blood of observation group were higher than those of normal control group while HDL-C and ApA1 contents were lower than those of normal control group;endothelial injury index ET-1 content in serum was higher than that of normal control group while NO content was lower than that of normal control group;inflammatory response indexes CRP, IL-1β, IL-6 and IL-17 contents in serum were higher than those of normal control group. Pearson test showed that serum E-FABP content in children with metabolic syndrome was directly correlated with glucose lipid metabolism, endothelial injury and inflammatory response indexes. Conclusion: Serum E-FABP content increases abnormally in children with metabolic syndrome and is directly correlated with the abnormal degree of glucose lipid metabolism, endothelial injury and inflammatory response.展开更多
文摘Objective: To study the correlation of serum E-FABP content with glucose lipid metabolism, endothelial injury and inflammatory response in children with metabolic syndrome. Methods:A total of 49 children with metabolic syndrome who were treated in the hospital between January 2016 and February 2017 were collected as observation group, and 50 healthy children who received vaccination in the hospital over the same period were collected as normal control group. Serum levels of E-FABP as well as glucose lipid metabolism, endothelial injury and inflammatory response indexes of two groups of subjects were detected, and the Pearson test was used to evaluate the correlation of E-FABP content with glucose lipid metabolism, endothelial injury and inflammatory response. Results: Serum E-FABP content of observation group was higher than that of control group;glucose metabolism indexes FBG and HOMA-IR levels in peripheral blood were higher than those of normal control group;lipid metabolism indexes TC, LDL-C and ApB contents in peripheral blood of observation group were higher than those of normal control group while HDL-C and ApA1 contents were lower than those of normal control group;endothelial injury index ET-1 content in serum was higher than that of normal control group while NO content was lower than that of normal control group;inflammatory response indexes CRP, IL-1β, IL-6 and IL-17 contents in serum were higher than those of normal control group. Pearson test showed that serum E-FABP content in children with metabolic syndrome was directly correlated with glucose lipid metabolism, endothelial injury and inflammatory response indexes. Conclusion: Serum E-FABP content increases abnormally in children with metabolic syndrome and is directly correlated with the abnormal degree of glucose lipid metabolism, endothelial injury and inflammatory response.
