Objective： To investigate the expressions of caveolin-1, E-cadherin and β-catenin in gastric carcinoma, precancerous gastric and chronic non-atrophic gastritis tissues, and evaluate the correlation of these expressi...Objective： To investigate the expressions of caveolin-1, E-cadherin and β-catenin in gastric carcinoma, precancerous gastric and chronic non-atrophic gastritis tissues, and evaluate the correlation of these expressions with the development of gastric cancer. Methods： The expressions of caveolin-1, E-cadherin and β-catenin were detected by biotin-streptavidinperoxidase （SP） immunohistochemistry on 58 gastric cancer tissues, 40 precancerous gastric tissues and 42 chronic non-atrophic gastritis tissues. The correlation between the expressions of caveolin-1, E-cadherin and β-catenin, and the clinicopathologic parameters of gastric cancer was analyzed retrospectively. Results： The positive rates of caveolin-1 and E-cadherin expressions in gastric carcinoma were significantly lower than precancerous gastric and chronic non-atrophic gastritis tissues （P〈0.01）. An abnormal rate of β-catenin expression in gastric carcinoma was higher than precancerous gastric and chronic non-atrophic gastritis tissues （P〈0.01）. Moreover, low expressions of caveolin-1, E-cadherin and β-catenin correlated with tumor size, depth of invasion, lymph node metastasis and TNM stage （P〈0.05）. The positive rates of caveolin-1 and E-cadherin expressions decreased （P〈0.01）, while an abnormal rate of β-catenin expression increased inversely, with the degree of atypical hyperplasia （P〈0.01）. Caveolin-1 expression correlated positively with E-cadherin （r=0.41, P〈0.05）. Caveolin-1 （r=-0.36, P〈0.05） and E-cadherin （r=-0.45, P〈0.05） expressions negatively correlated with abnormal β-catenin expression. Conclusion： These results suggested that dysregulated expressions of caveolin-1, E-cadherin and β-catenin correlated with the development of gastric cancer and its biological behavior.展开更多
BACKGROUND:p130Cas(p130Crk-associated substance) is a junction protein that is important to the adhesion between cytoskeleton and extracellular matrix.Also the adhesion molecules E-cadherin andβ-catenin play importan...BACKGROUND:p130Cas(p130Crk-associated substance) is a junction protein that is important to the adhesion between cytoskeleton and extracellular matrix.Also the adhesion molecules E-cadherin andβ-catenin play important roles in the invasiveness of carcinoma.This study was undertaken to investigate the effects of p130Cas E-cadherin andβ-catenin on the invasion,metastasis and prognosis of hepatocellular carcinoma(HCC). METHODS:Immunohistochemistry was used to evaluate the expression of p130Cas,E-cadherin,andβ-catenin in 40 patients with HCC.All patients were followed up postoperatively,and the relationship between expression and clinicopathological prognostic parameters was analyzed RESULTS:The positive expression rates of p130Cas and E-cadherin in HCC tissue(n=40)were 62.50%and 55.00% but in normal liver tissue 10%,and 100%,respectively (P<0.05).The abnormal expression rate ofβ-catenin in HCC tissue was 70%,while in normal liver tissue it was 13.33%(P<0.05).The positive rate of p130Cas was correlated with lymph node invasion,pathological stage TNM stage,and a worse prognosis,but not with gender age,HBV infection,hepatic cirrhosis,alpha-fetoprotein (AFP)level before operation,and tumor diameter Similarly,the expression of E-cadherin andβ-catenin was correlated with lymph node invasion,pathological stage,TNM stage,and worse prognosis,but not with gender,age,HBV infection,hepatic cirrhosis,AFP level before operation,and tumor size.Correlations were found between p130Cas and abnormal E-cadherin/β-catenin expression(P<0.001 and<0.05,respectively).CONCLUSIONS:In HCC,there is a negative correlation between the positive expression of p130Cas and the normal expression of the adhesion molecules E-cadherin/β-catenin, and p130Cas plays important roles in the invasion, metastasis and prognosis of HCC.p130Cas may be involved in alterating the structure and function of E-cadherin/ β-catenin,by regulating tyrosine phosphorylation via the p130Cas-Src signal pathway.展开更多
Objective:1.To evaluate E-cadherin expression and its clinical significance in pancreatic adenocarcinoma; 2. To further clarify if deregulation of the E-cadherin/β-catenin complex might be the cause for cytoplasmic a...Objective:1.To evaluate E-cadherin expression and its clinical significance in pancreatic adenocarcinoma; 2. To further clarify if deregulation of the E-cadherin/β-catenin complex might be the cause for cytoplasmic accumulation of β-catenin. Methods:We investigated the expression pattern of E-cadherin and β-catenin on serial sections from 41 pancreatic cancers. Precipitation and Western blot analysis were performed in 12 tumors with anti-E-cadherin antibody. Results:Reduced or entirely negative expression was found in 65.9%(27 of 41) of the tumors studied; 24 of 41 tumors exhibited increased cytoplasmic immunoreactivity. Interestingly, we found a clear inverse correlation between membranous and cytoplasmic expression of E-cadherin in 43 tumors(P<0.0005).The prognosis was poor with reduced tumor E-cadherin expression (P<0.05) but not with other clinicopathological parameters (age, sex, tumor grading and TNM stage); Analysis of serial sections stained with anti-β-catenin antibody revealed a marked correlation between aberrant expression of E-cadherin and ectopic accumulation of β-catenin(P<0.01).Immunoprecipitation of lysates from pancreatic adenocarcinomas and normal pancreas revealed a clear reduced coprecipitation activity of E-cadherin and β-catenin in the carcinoma samples.Conclusions:The finding, that the loss of membranous E-cadherin correlates significantly with cytoplasmic β-catenin expression, which is a factor for poor prognosis, makes a relationship between E-cadherin dysfunction, deregulated β-catenin and activated Tcf transcription possible.展开更多
This study was aimed to investigate the expressions of E-cadherin, p120 ctn, β-catenin and NF-κB in ulcerative colitis（UC） tissues and the implications of their expressions in the pathogenesis of UC. The expressio...This study was aimed to investigate the expressions of E-cadherin, p120 ctn, β-catenin and NF-κB in ulcerative colitis（UC） tissues and the implications of their expressions in the pathogenesis of UC. The expressions of E-cadherin, p120 ctn, β-catenin and NF-κB were detected by immunohistochemistry, and those of p120 ctn and NF-κB by Western blotting in 23 cases of UC and 17 cases of normal colonic tissues. The relationship between the expression of E-cadherin or NF-κB and that of p120 ctn was analyzed by Spearman rank correlation analysis. The results showed that in UC and normal colonic groups, the abnormal expression rate of E-cadherin, p120 ctn, β-catenin, and NF-κB was 52.2% vs. 0（P〈0.05）, 73.9% vs. 23.5%（P〈0.05）, 65.2% vs. 17.6%（P〈0.05） and 78.4% vs. 23.5%（P〈0.05）, respectively. p120 ctn expression was positively correlated with E-cadherin expression（r=0.404, P〈0.05）, but negatively with nuclear NF-κB expression（r= – 0.347, P〈0.05）. Western blotting showed that as compared with the normal controls, the p120 ctn protein level was significantly decreased（P〈0.05）, whereas the NF-κB protein level was increased（P〈0.05） in UC tissues. It was concluded that in the colonic tissues of UC patients, the expressions of E-cadherin, p120 ctn and β-catenin are decreased, suggesting the mucosal barrier is impaired in UC. Moreover, NF-κB is increased and activated in the UC tissues, resulting in the inflammation in UC. p120 ctn may influence the UC development through modulating intercellular adhesion and inflammatory response.展开更多
Objective: To investigate the significance of abnormal E-cadherin and β-catenin expression in pancreatic intraepithelial neoplasia (PanIN) and pancreatic adenocarcinoma. Methods:Pancreatic samples of 156 cases were r...Objective: To investigate the significance of abnormal E-cadherin and β-catenin expression in pancreatic intraepithelial neoplasia (PanIN) and pancreatic adenocarcinoma. Methods:Pancreatic samples of 156 cases were retrospectively studied from surgery and autopsy in Changhai hospital from January 2001 to December 2003, from which tissue microarray blocks containing 129 PanIN-1A lesions, 104 PanIN-1B lesions, 22 PanIN-2 lesions, 11 PanIN-3 lesions, and 121 pancreatic ductal adenocarcinomas and corresponding paracancerous tissues were constructed. EnVision method of immunohistochemistry was used to detect the E-cadherin and β-catenin expression. The correlation between the abnormal E-cadherin and β-catenin expression and clinicopathological parameters was analysed. Results: The rate of E-cadherin abnormal expression was significant in ductal adenocarcinomas compared with the PanIN lesions and normal ducts(64.5%,32.3%,0%), moreover, the rate of E-cadherin abnormal expression was in relation to differentiation, lymph node metastasis and perineural invasion of pancreatic adenocarcinoma(P<0.05). There was remarkable increase in the E-cadherin cytoplasmic expression in PanIN lesions and ductal adenocarcinomas compared with normal ducts. The rate of β-catenin abnormal expression was found to be related with lymph node metastasis and perineural invasion of pancreatic adenocarcinoma(P<0.05). The expression of β-catenin cytoplasm and/or nucleus was significant in high-grade PanIN lesions and ductal adenocarcinomas compared with low grade PanIN lesions or normal ducts(P<0.05). There was a positive relationship between the E-cadherin and β-catenin expression in PanIN lesions and ductal adenocarcinomas (P<0.01, P<0.05). Conclusion: There is aberration in the expression of the E-cadherin and β-catenin in PanIN lesions and ductal adenocarcinomas, suggesting the E-cadherin and β-catenin changes is not only related with the biological action and prognosis, but also involved in pancreatic carcinogenesis.展开更多
基金supported by Zhejiang Provincial Natural Science Foundation (No. Y206750)Zhejiang Foundation for Development of Science and Technology, China (No. 2008C33066)Wenzhou Foundation for Development of Science and Technology,China (No. H20060026)
文摘Objective： To investigate the expressions of caveolin-1, E-cadherin and β-catenin in gastric carcinoma, precancerous gastric and chronic non-atrophic gastritis tissues, and evaluate the correlation of these expressions with the development of gastric cancer. Methods： The expressions of caveolin-1, E-cadherin and β-catenin were detected by biotin-streptavidinperoxidase （SP） immunohistochemistry on 58 gastric cancer tissues, 40 precancerous gastric tissues and 42 chronic non-atrophic gastritis tissues. The correlation between the expressions of caveolin-1, E-cadherin and β-catenin, and the clinicopathologic parameters of gastric cancer was analyzed retrospectively. Results： The positive rates of caveolin-1 and E-cadherin expressions in gastric carcinoma were significantly lower than precancerous gastric and chronic non-atrophic gastritis tissues （P〈0.01）. An abnormal rate of β-catenin expression in gastric carcinoma was higher than precancerous gastric and chronic non-atrophic gastritis tissues （P〈0.01）. Moreover, low expressions of caveolin-1, E-cadherin and β-catenin correlated with tumor size, depth of invasion, lymph node metastasis and TNM stage （P〈0.05）. The positive rates of caveolin-1 and E-cadherin expressions decreased （P〈0.01）, while an abnormal rate of β-catenin expression increased inversely, with the degree of atypical hyperplasia （P〈0.01）. Caveolin-1 expression correlated positively with E-cadherin （r=0.41, P〈0.05）. Caveolin-1 （r=-0.36, P〈0.05） and E-cadherin （r=-0.45, P〈0.05） expressions negatively correlated with abnormal β-catenin expression. Conclusion： These results suggested that dysregulated expressions of caveolin-1, E-cadherin and β-catenin correlated with the development of gastric cancer and its biological behavior.
文摘BACKGROUND:p130Cas(p130Crk-associated substance) is a junction protein that is important to the adhesion between cytoskeleton and extracellular matrix.Also the adhesion molecules E-cadherin andβ-catenin play important roles in the invasiveness of carcinoma.This study was undertaken to investigate the effects of p130Cas E-cadherin andβ-catenin on the invasion,metastasis and prognosis of hepatocellular carcinoma(HCC). METHODS:Immunohistochemistry was used to evaluate the expression of p130Cas,E-cadherin,andβ-catenin in 40 patients with HCC.All patients were followed up postoperatively,and the relationship between expression and clinicopathological prognostic parameters was analyzed RESULTS:The positive expression rates of p130Cas and E-cadherin in HCC tissue(n=40)were 62.50%and 55.00% but in normal liver tissue 10%,and 100%,respectively (P<0.05).The abnormal expression rate ofβ-catenin in HCC tissue was 70%,while in normal liver tissue it was 13.33%(P<0.05).The positive rate of p130Cas was correlated with lymph node invasion,pathological stage TNM stage,and a worse prognosis,but not with gender age,HBV infection,hepatic cirrhosis,alpha-fetoprotein (AFP)level before operation,and tumor diameter Similarly,the expression of E-cadherin andβ-catenin was correlated with lymph node invasion,pathological stage,TNM stage,and worse prognosis,but not with gender,age,HBV infection,hepatic cirrhosis,AFP level before operation,and tumor size.Correlations were found between p130Cas and abnormal E-cadherin/β-catenin expression(P<0.001 and<0.05,respectively).CONCLUSIONS:In HCC,there is a negative correlation between the positive expression of p130Cas and the normal expression of the adhesion molecules E-cadherin/β-catenin, and p130Cas plays important roles in the invasion, metastasis and prognosis of HCC.p130Cas may be involved in alterating the structure and function of E-cadherin/ β-catenin,by regulating tyrosine phosphorylation via the p130Cas-Src signal pathway.
文摘Objective:1.To evaluate E-cadherin expression and its clinical significance in pancreatic adenocarcinoma; 2. To further clarify if deregulation of the E-cadherin/β-catenin complex might be the cause for cytoplasmic accumulation of β-catenin. Methods:We investigated the expression pattern of E-cadherin and β-catenin on serial sections from 41 pancreatic cancers. Precipitation and Western blot analysis were performed in 12 tumors with anti-E-cadherin antibody. Results:Reduced or entirely negative expression was found in 65.9%(27 of 41) of the tumors studied; 24 of 41 tumors exhibited increased cytoplasmic immunoreactivity. Interestingly, we found a clear inverse correlation between membranous and cytoplasmic expression of E-cadherin in 43 tumors(P<0.0005).The prognosis was poor with reduced tumor E-cadherin expression (P<0.05) but not with other clinicopathological parameters (age, sex, tumor grading and TNM stage); Analysis of serial sections stained with anti-β-catenin antibody revealed a marked correlation between aberrant expression of E-cadherin and ectopic accumulation of β-catenin(P<0.01).Immunoprecipitation of lysates from pancreatic adenocarcinomas and normal pancreas revealed a clear reduced coprecipitation activity of E-cadherin and β-catenin in the carcinoma samples.Conclusions:The finding, that the loss of membranous E-cadherin correlates significantly with cytoplasmic β-catenin expression, which is a factor for poor prognosis, makes a relationship between E-cadherin dysfunction, deregulated β-catenin and activated Tcf transcription possible.
基金supported by a grant from the National Natural Science Foundation of China(No.81070009)
文摘This study was aimed to investigate the expressions of E-cadherin, p120 ctn, β-catenin and NF-κB in ulcerative colitis（UC） tissues and the implications of their expressions in the pathogenesis of UC. The expressions of E-cadherin, p120 ctn, β-catenin and NF-κB were detected by immunohistochemistry, and those of p120 ctn and NF-κB by Western blotting in 23 cases of UC and 17 cases of normal colonic tissues. The relationship between the expression of E-cadherin or NF-κB and that of p120 ctn was analyzed by Spearman rank correlation analysis. The results showed that in UC and normal colonic groups, the abnormal expression rate of E-cadherin, p120 ctn, β-catenin, and NF-κB was 52.2% vs. 0（P〈0.05）, 73.9% vs. 23.5%（P〈0.05）, 65.2% vs. 17.6%（P〈0.05） and 78.4% vs. 23.5%（P〈0.05）, respectively. p120 ctn expression was positively correlated with E-cadherin expression（r=0.404, P〈0.05）, but negatively with nuclear NF-κB expression（r= – 0.347, P〈0.05）. Western blotting showed that as compared with the normal controls, the p120 ctn protein level was significantly decreased（P〈0.05）, whereas the NF-κB protein level was increased（P〈0.05） in UC tissues. It was concluded that in the colonic tissues of UC patients, the expressions of E-cadherin, p120 ctn and β-catenin are decreased, suggesting the mucosal barrier is impaired in UC. Moreover, NF-κB is increased and activated in the UC tissues, resulting in the inflammation in UC. p120 ctn may influence the UC development through modulating intercellular adhesion and inflammatory response.
基金Acknowledgment This work was supported by grants from the National Nature Science Foundation of China （No.31071091 No.30971570） and Department of Education key project of Hunan Province, China （09A035 and 07B029）.
基金Supported by Changhai Hospital Subject ConstructProgram.
文摘Objective: To investigate the significance of abnormal E-cadherin and β-catenin expression in pancreatic intraepithelial neoplasia (PanIN) and pancreatic adenocarcinoma. Methods:Pancreatic samples of 156 cases were retrospectively studied from surgery and autopsy in Changhai hospital from January 2001 to December 2003, from which tissue microarray blocks containing 129 PanIN-1A lesions, 104 PanIN-1B lesions, 22 PanIN-2 lesions, 11 PanIN-3 lesions, and 121 pancreatic ductal adenocarcinomas and corresponding paracancerous tissues were constructed. EnVision method of immunohistochemistry was used to detect the E-cadherin and β-catenin expression. The correlation between the abnormal E-cadherin and β-catenin expression and clinicopathological parameters was analysed. Results: The rate of E-cadherin abnormal expression was significant in ductal adenocarcinomas compared with the PanIN lesions and normal ducts(64.5%,32.3%,0%), moreover, the rate of E-cadherin abnormal expression was in relation to differentiation, lymph node metastasis and perineural invasion of pancreatic adenocarcinoma(P<0.05). There was remarkable increase in the E-cadherin cytoplasmic expression in PanIN lesions and ductal adenocarcinomas compared with normal ducts. The rate of β-catenin abnormal expression was found to be related with lymph node metastasis and perineural invasion of pancreatic adenocarcinoma(P<0.05). The expression of β-catenin cytoplasm and/or nucleus was significant in high-grade PanIN lesions and ductal adenocarcinomas compared with low grade PanIN lesions or normal ducts(P<0.05). There was a positive relationship between the E-cadherin and β-catenin expression in PanIN lesions and ductal adenocarcinomas (P<0.01, P<0.05). Conclusion: There is aberration in the expression of the E-cadherin and β-catenin in PanIN lesions and ductal adenocarcinomas, suggesting the E-cadherin and β-catenin changes is not only related with the biological action and prognosis, but also involved in pancreatic carcinogenesis.
