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犬2型腺病毒SY株E1区、E3区的克隆与序列分析
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作者 邱薇 夏咸柱 +4 位作者 范泉水 扈荣良 王雷 高玉伟 张守峰 《中国预防兽医学报》 CAS CSCD 北大核心 2004年第5期339-342,共4页
根据犬2型腺病毒Toronto A26/61株的全基因序列物理图谱,确定E1、E3区位置,从CAV-2SY株的细胞培养物中提取了CAV-2全基因组DNA,经电泳检测其完整性并用多个限制性内切酶鉴定后,用EcoRⅠ和Kpn Ⅰ分别进行酶切,回收含E1区和E3区的相应片段... 根据犬2型腺病毒Toronto A26/61株的全基因序列物理图谱,确定E1、E3区位置,从CAV-2SY株的细胞培养物中提取了CAV-2全基因组DNA,经电泳检测其完整性并用多个限制性内切酶鉴定后,用EcoRⅠ和Kpn Ⅰ分别进行酶切,回收含E1区和E3区的相应片段,分别克隆到质粒pGEM-3zf和pBluescriptⅡKs(+)中,得到含E1区的重组质粒pGE1和含E3区的重组质粒pBE3-2。对获得的两个片段进行了序列测定和分析,结果表明,犬2型腺病毒SY株E1、E3基因的核苷酸序列与Toronto株的相应序列完全相同,其同源性均为100%。 展开更多
关键词 犬2型腺病毒 e1、e3 克隆 序列分析
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Intelligent and Predictive Vehicular Networks
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作者 Schmidt Shilukobo Chintu Richard Anthony +1 位作者 Maryam Roshanaei Constantinos Ierotheou 《Intelligent Control and Automation》 2014年第2期60-71,共12页
Seeking shortest travel times through smart algorithms may not only optimize the travel times but also reduce carbon emissions, such as CO2, CO and Hydro-Carbons. It can also result in reduced driver frustrations and ... Seeking shortest travel times through smart algorithms may not only optimize the travel times but also reduce carbon emissions, such as CO2, CO and Hydro-Carbons. It can also result in reduced driver frustrations and can increase passenger expectations of consistent travel times, which in turn points to benefits in overall planning of day schedules. Fuel consumption savings are another benefit from the same. However, attempts to elect the shortest path as an assumption of quick travel times, often work counter to the very objective intended and come with the risk of creating a “Braess Paradox” which is about congestion resulting when several drivers attempt to elect the same shortest route. The situation that arises has been referred to as the price of anarchy! We propose algorithms that find multiple shortest paths between an origin and a destination. It must be appreciated that these will not yield the exact number of Kilometers travelled, but favourable weights in terms of travel times so that a reasonable allowable time difference between the multiple shortest paths is attained when the same Origin and Destinations are considered and favourable responsive routes are determined as variables of traffic levels and time of day. These routes are selected on the paradigm of route balancing, re-routing algorithms and traffic light intelligence all coming together to result in optimized consistent travel times whose benefits are evenly spread to all motorist, unlike the Entropy balanced k shortest paths (EBkSP) method which favours some motorists on the basis of urgency. This paper proposes a Fully Balanced Multiple-Candidate shortest path (FBMkP) by which we model in SUMO to overcome the computational overhead of assigning priority differently to each travelling vehicle using intelligence at intersections and other points on the vehicular network. The FBMkP opens up traffic by fully balancing the whole network so as to benefit every motorist. Whereas the EBkSP reserves some routes for cars on high priority, our algorithm distributes the benefits of smart routing to all vehicles on the network and serves the road side units such as induction loops and detectors from having to remember the urgency of each vehicle. Instead, detectors and induction loops simply have to poll the destination of the vehicle and not any urgency factor. The minimal data being processed significantly reduce computational times and the benefits all vehicles. The multiple-candidate shortest paths selected on the basis of current traffic status on each possible route increase the efficiency. Routes are fewer than vehicles so possessing weights of routes is smarter than processing individual vehicle weights. This is a multi-objective function project where improving one factor such as travel times improves many more cost, social and environmental factors. 展开更多
关键词 Simulation of Urban Mobility SUMO Duarouter Fully Balanced Multiple-Candidate Shortest Paths (FBMKP) e1 Induction Loop e3 Detector Re-Routing Braess PARADOX TRAFFIC CONTROL INTeLLIGeNT (TraCI) Partially Re-Routed Shortest Path Method TRAFFIC Light CONTROL FBMKP
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植物泛素化系统 被引量:1
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作者 杜秋丽 李莉 《济宁学院学报》 2011年第3期52-55,共4页
植物生长发育的许多过程都受到泛素-蛋白酶体途径的调控.泛素-蛋白酶体途径包括两个连续的过程:泛素化系统将底物蛋白泛素化;26s蛋白酶体降解泛素化的靶蛋白.其中泛素化系统对底物的泛素化是通过三种关键酶即泛素活化酶、泛素结合酶和... 植物生长发育的许多过程都受到泛素-蛋白酶体途径的调控.泛素-蛋白酶体途径包括两个连续的过程:泛素化系统将底物蛋白泛素化;26s蛋白酶体降解泛素化的靶蛋白.其中泛素化系统对底物的泛素化是通过三种关键酶即泛素活化酶、泛素结合酶和泛素连接酶的连接级联反应完成的.这三类酶通过泛素介导的蛋白质选择性降解参与植物光形态建成、配子体发育、花发育、种子发育、激素的信号转导、抗逆、抗虫等方面的调控. 展开更多
关键词 泛素蛋白酶体途径 泛素化系统 e1泛素活化酶 e2泛素结合酶 e3泛素连接酶
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