The aim of the present study was to compare one-step method to EDC/NHS crosslinking （EDC/NHS group） and one-step simultaneous method to EDC/NHS crosslinking and heparin immobilization （EDC/NHS- Heparin group） in i...The aim of the present study was to compare one-step method to EDC/NHS crosslinking （EDC/NHS group） and one-step simultaneous method to EDC/NHS crosslinking and heparin immobilization （EDC/NHS- Heparin group） in improving physiochemical and biological properties of native collagen sponge （Control group）. Modified collagen sponge overcome the disadvantages of native collagen sponge. IR spectra suggest the change of the functional groups. DSC data indicate that the stability of caloric transformation in EDC/NHS group is slightly higher than that of EDC/NHS-Heparin group. The crosslinking degree, stability against enzymes, stability in morphologically and biomechanical properties of EDC/NHS-Heparin group are higher than those of EDC/NHS group, whereas, the water-binding capacity in EDC/NHS-Heparin group is lower than that of EDC/NHS group. HUVECs in EDC/NHS-Heparin group scaffold proliferate fast, migrate well and distribute uniformly. One-step simultaneous method gains the better effects in above aspects, heparinized collagen matrices increase in angiogenic potential and suit for defect repairing and tissue engineering.展开更多
The dispersion characteristics of a class of closed dielectric waveguides are investigated by themethod which combines the building-block approach of multimode network theory with the rigorous modematching procedure.S...The dispersion characteristics of a class of closed dielectric waveguides are investigated by themethod which combines the building-block approach of multimode network theory with the rigorous modematching procedure.Several numerical examples for difrerent guiding structures have been given by the ap-proach.The same structures are also analyzed with the finite element method and the EDC method forcomparison.The calculations show that the present approach yields as highly accurate results as the finite ele-ment method while almost retaining the simplicity of the EDC method,and justify the utility of the presentmethod.展开更多
基金Funded by the National Natural Science Foundation of China (10832012)the Natural Science Foudation of Tianjin city(08JCYBJC03400)
文摘The aim of the present study was to compare one-step method to EDC/NHS crosslinking （EDC/NHS group） and one-step simultaneous method to EDC/NHS crosslinking and heparin immobilization （EDC/NHS- Heparin group） in improving physiochemical and biological properties of native collagen sponge （Control group）. Modified collagen sponge overcome the disadvantages of native collagen sponge. IR spectra suggest the change of the functional groups. DSC data indicate that the stability of caloric transformation in EDC/NHS group is slightly higher than that of EDC/NHS-Heparin group. The crosslinking degree, stability against enzymes, stability in morphologically and biomechanical properties of EDC/NHS-Heparin group are higher than those of EDC/NHS group, whereas, the water-binding capacity in EDC/NHS-Heparin group is lower than that of EDC/NHS group. HUVECs in EDC/NHS-Heparin group scaffold proliferate fast, migrate well and distribute uniformly. One-step simultaneous method gains the better effects in above aspects, heparinized collagen matrices increase in angiogenic potential and suit for defect repairing and tissue engineering.
基金Supported by the National Nature Seience Foundation of China
文摘The dispersion characteristics of a class of closed dielectric waveguides are investigated by themethod which combines the building-block approach of multimode network theory with the rigorous modematching procedure.Several numerical examples for difrerent guiding structures have been given by the ap-proach.The same structures are also analyzed with the finite element method and the EDC method forcomparison.The calculations show that the present approach yields as highly accurate results as the finite ele-ment method while almost retaining the simplicity of the EDC method,and justify the utility of the presentmethod.
