Background:Recurrent miscarriage(RM)affects an estimated 1-3%of couples attempting to conceive,and its molecular components stay ineffectively caught on.This study aims to explore potential therapeutic targets for RM ...Background:Recurrent miscarriage(RM)affects an estimated 1-3%of couples attempting to conceive,and its molecular components stay ineffectively caught on.This study aims to explore potential therapeutic targets for RM by examining gene expression patterns and biological pathways in both mouse and human RM models.Meanwhile,explore relevant traditional Chinese medicine(TCM)components targeting potential therapeutic targets.Methods:We utilized the GSE211251 mouse and the GSE26787 human datasets,employing gene set enrichment analysis and gene metaphysics analysis to examine differentially expressed genes and enriched pathways.Single-cell RNA analysis uncovered cellular heterogeneity and arranged pharmacology-mapped potential drug-target intelligence.We employed molecular docking strategies to assess the affinity of TCM components for key proteins.Results:In the mouse model,genes such as Ly6f1 and Gpr26 were upregulated,while Stc5a and Galca exhibited downregulation.Gene set enrichment analysis identified key pathways,including the tumor necrosis factor-mediated signaling pathway.In human samples,Gene Ontology analysis highlighted processes such as apoptosis and cell adhesion.Single-cell RNA analysis revealed distinct cellular populations between normal and RM samples.Systems pharmacology identified C-X-C motif chemokine receptor 4(CXCR4)and endothelin 1(EDN1)as potential key targets,and molecular docking confirmed that stearic acid from TCM appears to regulate these proteins.Conclusion:This study presents a comprehensive analysis of the genetic and cellular underpinnings of RM,identifying CXCR4 and EDN1 as promising therapeutic targets.Stearic acid from TCM could provide targeted treatment by modulating these key proteins,paving the way for new RM treatment strategies.展开更多
文章主要针对基于ECS(Edns-Client-Subnet)机制的智能EDNS(Extension Mechanisms for DNS)进行了相关研究,对智能EDNS的技术原理、报文形式、主要用途以及实际应用进行了介绍,通过对比分析智能EDNS机制与传统DNS的优势,结合实际应用中CD...文章主要针对基于ECS(Edns-Client-Subnet)机制的智能EDNS(Extension Mechanisms for DNS)进行了相关研究,对智能EDNS的技术原理、报文形式、主要用途以及实际应用进行了介绍,通过对比分析智能EDNS机制与传统DNS的优势,结合实际应用中CDN调度存在的不足,提出了改进版的基于ECS机制的CDN调度优化方案,并就实现过程中的关键要点进行阐述。展开更多
目的探讨参芎注射液联合辛伐他汀对EDN血液流变学、肾功能的影响。方法将2006年01月至2011年10月笔者收治94例EDN患者了随机分为治疗组48例,对照组46例,两组均采用控制饮食、降血糖常规治疗。治疗组在此基础上予参芎注射液200mL静脉滴注...目的探讨参芎注射液联合辛伐他汀对EDN血液流变学、肾功能的影响。方法将2006年01月至2011年10月笔者收治94例EDN患者了随机分为治疗组48例,对照组46例,两组均采用控制饮食、降血糖常规治疗。治疗组在此基础上予参芎注射液200mL静脉滴注2周,间隔2周后再静脉滴注2周,予辛伐他汀20mg po qn疗程6周。结果治疗组血液流变学各项指标显著下降(P<0.01),血肌酐,尿素氮,尿白蛋白排泄率显著下降(P<0.01),且明显优于对照组(P<0.01/P<0.05)。结论参芎注射液联合辛伐他汀能显著减轻EDN的肾损害,改善肾功能及血液流变学指标。展开更多
基金support from the Ningxia Hui Autonomous Region Key Research and Development Program(Project No.2021BEG03041).
文摘Background:Recurrent miscarriage(RM)affects an estimated 1-3%of couples attempting to conceive,and its molecular components stay ineffectively caught on.This study aims to explore potential therapeutic targets for RM by examining gene expression patterns and biological pathways in both mouse and human RM models.Meanwhile,explore relevant traditional Chinese medicine(TCM)components targeting potential therapeutic targets.Methods:We utilized the GSE211251 mouse and the GSE26787 human datasets,employing gene set enrichment analysis and gene metaphysics analysis to examine differentially expressed genes and enriched pathways.Single-cell RNA analysis uncovered cellular heterogeneity and arranged pharmacology-mapped potential drug-target intelligence.We employed molecular docking strategies to assess the affinity of TCM components for key proteins.Results:In the mouse model,genes such as Ly6f1 and Gpr26 were upregulated,while Stc5a and Galca exhibited downregulation.Gene set enrichment analysis identified key pathways,including the tumor necrosis factor-mediated signaling pathway.In human samples,Gene Ontology analysis highlighted processes such as apoptosis and cell adhesion.Single-cell RNA analysis revealed distinct cellular populations between normal and RM samples.Systems pharmacology identified C-X-C motif chemokine receptor 4(CXCR4)and endothelin 1(EDN1)as potential key targets,and molecular docking confirmed that stearic acid from TCM appears to regulate these proteins.Conclusion:This study presents a comprehensive analysis of the genetic and cellular underpinnings of RM,identifying CXCR4 and EDN1 as promising therapeutic targets.Stearic acid from TCM could provide targeted treatment by modulating these key proteins,paving the way for new RM treatment strategies.
文摘文章主要针对基于ECS(Edns-Client-Subnet)机制的智能EDNS(Extension Mechanisms for DNS)进行了相关研究,对智能EDNS的技术原理、报文形式、主要用途以及实际应用进行了介绍,通过对比分析智能EDNS机制与传统DNS的优势,结合实际应用中CDN调度存在的不足,提出了改进版的基于ECS机制的CDN调度优化方案,并就实现过程中的关键要点进行阐述。
文摘目的探讨参芎注射液联合辛伐他汀对EDN血液流变学、肾功能的影响。方法将2006年01月至2011年10月笔者收治94例EDN患者了随机分为治疗组48例,对照组46例,两组均采用控制饮食、降血糖常规治疗。治疗组在此基础上予参芎注射液200mL静脉滴注2周,间隔2周后再静脉滴注2周,予辛伐他汀20mg po qn疗程6周。结果治疗组血液流变学各项指标显著下降(P<0.01),血肌酐,尿素氮,尿白蛋白排泄率显著下降(P<0.01),且明显优于对照组(P<0.01/P<0.05)。结论参芎注射液联合辛伐他汀能显著减轻EDN的肾损害,改善肾功能及血液流变学指标。
