In order to study the mechanism of estrogen and other events involved in the development of breast cancer,we observed the cell growth as well as the expression of p185 and epidermal growth factor(EGF) receptor in huma...In order to study the mechanism of estrogen and other events involved in the development of breast cancer,we observed the cell growth as well as the expression of p185 and epidermal growth factor(EGF) receptor in human breast cancer cell line SK-BR-3 in the presence of estradiol(E2) using flow cytometry. The results showed that E2 could act to expedite the proliferation of breast cancer cells and promote DNA synthesis , and that the percentage in S phase rose significantly (E2 group 26. 7?2. 5,control group 21. 2?2.1, P<0. 05). Under E2 action,p185 presented a reduced expression (E2 35?5. 6, control 61?13.1, P<0. 05) while the EGF receptor expression was greatly enhanced (E, 39?6. 9, control 21?5. 4,P<0. 05),suggesting that EGF may play a more important role in early development of breast cancer.展开更多
The epidermal growth factor （EGF） has been shown to promote the proliferation of various types of cells, to maintain the physiological function of the mucosa of the digestive tract, and to promote the healing of the...The epidermal growth factor （EGF） has been shown to promote the proliferation of various types of cells, to maintain the physiological function of the mucosa of the digestive tract, and to promote the healing of the gastric and duodenal ulcers. It has been expressed in many types of bacteria and yeasts. In this article, a bio-reactor was constructed, namely, the human EGF （hEGF） transgenic mini-tomato. On the basis of hEGF gene sequence, a tomato codon preference hEGF gene was chemically synthesized, and it was constructed into the plant expression vector pCAMBIA2300. The transgenic tomato plants containing gene hEGF were obtained through Agrobacterium-mediated transformation. The expression product was determined by radioimmunoassay （RIA） and showed a yield of 3.48± 1.01 ng/g fresh fruits. The intragastric gavage （ig） administration of the rhEGF-containing juice of the transgenic tomato （equivalent to 24 ng rhEGF per mouse a day） for 15 days could significantly protect mice against the alcohol-induced ulceration. The ulcer index, expressed as a degree of the stomach lesion, decreased from 42.20 ±18.13 to 16.25 ±9.57.展开更多
文摘In order to study the mechanism of estrogen and other events involved in the development of breast cancer,we observed the cell growth as well as the expression of p185 and epidermal growth factor(EGF) receptor in human breast cancer cell line SK-BR-3 in the presence of estradiol(E2) using flow cytometry. The results showed that E2 could act to expedite the proliferation of breast cancer cells and promote DNA synthesis , and that the percentage in S phase rose significantly (E2 group 26. 7?2. 5,control group 21. 2?2.1, P<0. 05). Under E2 action,p185 presented a reduced expression (E2 35?5. 6, control 61?13.1, P<0. 05) while the EGF receptor expression was greatly enhanced (E, 39?6. 9, control 21?5. 4,P<0. 05),suggesting that EGF may play a more important role in early development of breast cancer.
基金This work was supported by the Innovation Group Development Project of the Ministry of Education of China (No. IRT0435).
文摘The epidermal growth factor （EGF） has been shown to promote the proliferation of various types of cells, to maintain the physiological function of the mucosa of the digestive tract, and to promote the healing of the gastric and duodenal ulcers. It has been expressed in many types of bacteria and yeasts. In this article, a bio-reactor was constructed, namely, the human EGF （hEGF） transgenic mini-tomato. On the basis of hEGF gene sequence, a tomato codon preference hEGF gene was chemically synthesized, and it was constructed into the plant expression vector pCAMBIA2300. The transgenic tomato plants containing gene hEGF were obtained through Agrobacterium-mediated transformation. The expression product was determined by radioimmunoassay （RIA） and showed a yield of 3.48± 1.01 ng/g fresh fruits. The intragastric gavage （ig） administration of the rhEGF-containing juice of the transgenic tomato （equivalent to 24 ng rhEGF per mouse a day） for 15 days could significantly protect mice against the alcohol-induced ulceration. The ulcer index, expressed as a degree of the stomach lesion, decreased from 42.20 ±18.13 to 16.25 ±9.57.
