Hepatosteatosis is characterized by abnormal accumulation of triglycerides(TG),leading to prolonged and chronic inflammatory infiltration.To date,there is still a lack of effective and economical therapies for hepatos...Hepatosteatosis is characterized by abnormal accumulation of triglycerides(TG),leading to prolonged and chronic inflammatory infiltration.To date,there is still a lack of effective and economical therapies for hepatosteatosis.Oridonin(ORI)is a major bioactive component extracted from the traditional Chinese medicinal herb Rabdosia rubescens.In this paper,we showed that ORI exerted significant protective effects against hepatic steatosis,inflammation and fibrosis,which was dependent on LXRa signaling.It is reported that LXRa regulated lipid homeostasis between triglyceride(TG)and phosphatidylethanolamine(PE)by promoting ATGL and EPT1 expression.Therefore,we implemented the lipidomic strategy and luciferase reporter assay to verify that ORI contributed to the homeostasis of lipids via the regulation of the ATGL gene associated with TG hydrolysis and the EPT1 gene related to PE synthesis in a LXRadependent manner,and the results showed the TG reduction and PE elevation.In detail,hepatic TG overload and lipotoxicity were reversed after ORI treatment by modulating the ATGL and EPT1 genes,respectively.Taken together,the data provide mechanistic insights to explain the bioactivity of ORI in attenuating TG accumulation and cytotoxicity and introduce exciting opportunities for developing novel natural activators of the LXRa-ATGL/EPT1 axis for pharmacologically treating hepatosteatosis and metabolic disorders.展开更多
为了解同一流域不同水生态区EPT〔蜉蝣目（Ephemeroptera）、襀翅目（Plecoptera）、毛翅目（Trichoptera）〕这一敏感类群的时空分布差异及其影响因素,于2009年枯水期（5月）和丰水期（8月）分别对太子河流域3个水生态区进行了野外调查...为了解同一流域不同水生态区EPT〔蜉蝣目（Ephemeroptera）、襀翅目（Plecoptera）、毛翅目（Trichoptera）〕这一敏感类群的时空分布差异及其影响因素,于2009年枯水期（5月）和丰水期（8月）分别对太子河流域3个水生态区进行了野外调查.结果表明：太子河流域上游区域的水生态Ⅰ区EPT物种数（64种）显著高于中下游区域的水生态Ⅱ区和Ⅲ区,其中,仅毛翅目物种数在丰水期显著高于枯水期.典范对应分析（canonical correspondence analysis,CCA）结果显示,水生态Ⅰ区影响EPT群落分布的环境要素为电导率和水温,而水生态Ⅱ区、Ⅲ区则为电导率、φ（细沙）、ρ（BOD5）、ρ（TP）和ρ（NH3-N）.枯水期影响EPT群落分布的因素为电导率、建设用地面积所占比例和ρ（BOD5）,丰水期为电导率、ρ（SS）、耕地面积所占比例和水温.偏线性回归（partial linear regression,PLR）分析显示,水生态Ⅱ区物种和环境要素的模型总解释率最高（均约为0.60）,其他2个区次之（水生态Ⅰ区、Ⅲ区总解释率分别为0.13～0.43、0.13～0.53）,丰水期模型的总解释率为0.33～0.78,而枯水期模型的总解释率为0.43～0.71.相对于单一环境要素模型,不同类型环境要素的联合模型对太子河流域EPT群落空间变异具有更好的解释效力.展开更多
基金supported by the National Natural Science Foundation of China(Grant No.:81973388)Marine Economy Development Project of Guangdong Province(Project No.:GDNRC[2021]52)the Key Research and Development Program of Guangdong Province(Program No.:2020B1111030005).
文摘Hepatosteatosis is characterized by abnormal accumulation of triglycerides(TG),leading to prolonged and chronic inflammatory infiltration.To date,there is still a lack of effective and economical therapies for hepatosteatosis.Oridonin(ORI)is a major bioactive component extracted from the traditional Chinese medicinal herb Rabdosia rubescens.In this paper,we showed that ORI exerted significant protective effects against hepatic steatosis,inflammation and fibrosis,which was dependent on LXRa signaling.It is reported that LXRa regulated lipid homeostasis between triglyceride(TG)and phosphatidylethanolamine(PE)by promoting ATGL and EPT1 expression.Therefore,we implemented the lipidomic strategy and luciferase reporter assay to verify that ORI contributed to the homeostasis of lipids via the regulation of the ATGL gene associated with TG hydrolysis and the EPT1 gene related to PE synthesis in a LXRadependent manner,and the results showed the TG reduction and PE elevation.In detail,hepatic TG overload and lipotoxicity were reversed after ORI treatment by modulating the ATGL and EPT1 genes,respectively.Taken together,the data provide mechanistic insights to explain the bioactivity of ORI in attenuating TG accumulation and cytotoxicity and introduce exciting opportunities for developing novel natural activators of the LXRa-ATGL/EPT1 axis for pharmacologically treating hepatosteatosis and metabolic disorders.
文摘为了解同一流域不同水生态区EPT〔蜉蝣目（Ephemeroptera）、襀翅目（Plecoptera）、毛翅目（Trichoptera）〕这一敏感类群的时空分布差异及其影响因素,于2009年枯水期（5月）和丰水期（8月）分别对太子河流域3个水生态区进行了野外调查.结果表明：太子河流域上游区域的水生态Ⅰ区EPT物种数（64种）显著高于中下游区域的水生态Ⅱ区和Ⅲ区,其中,仅毛翅目物种数在丰水期显著高于枯水期.典范对应分析（canonical correspondence analysis,CCA）结果显示,水生态Ⅰ区影响EPT群落分布的环境要素为电导率和水温,而水生态Ⅱ区、Ⅲ区则为电导率、φ（细沙）、ρ（BOD5）、ρ（TP）和ρ（NH3-N）.枯水期影响EPT群落分布的因素为电导率、建设用地面积所占比例和ρ（BOD5）,丰水期为电导率、ρ（SS）、耕地面积所占比例和水温.偏线性回归（partial linear regression,PLR）分析显示,水生态Ⅱ区物种和环境要素的模型总解释率最高（均约为0.60）,其他2个区次之（水生态Ⅰ区、Ⅲ区总解释率分别为0.13～0.43、0.13～0.53）,丰水期模型的总解释率为0.33～0.78,而枯水期模型的总解释率为0.43～0.71.相对于单一环境要素模型,不同类型环境要素的联合模型对太子河流域EPT群落空间变异具有更好的解释效力.
